BGC0001101: leinamycin biosynthetic gene cluster from Streptomyces atroolivaceus
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Location: 1 - 135,638 nt. (total: 135,638 nt).
This entry is originally from NCBI GenBank AF484556.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001101
Short description leinamycin biosynthetic gene cluster from Streptomyces atroolivaceus
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
AF484556.1
via Knock-out studies, Enzymatic assays
Compounds
  • leinamycin
Species Streptomyces atroolivaceus [taxonomy]
References
Chemical products information
leinamycin Evidence:
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C22H26N2O6S3
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAN85479.1
 3489 - 4358 (+) putative antibiotic resistance protein
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    • AAN85480.1
    		 5108 - 6616 (+) putative FAD-dependent oxygenase
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      • AAN85481.1
      		 7431 - 11144 (+) subtilisin-like secreted protease
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        • AAN85482.1
        		 11024 - 11812 (+) hypothetical protein
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          • AAN85483.1
          		 11809 - 13014 (+) putative NADH dehydrogenase II
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            • AAN85484.1
            		 13011 - 13931 (+) RNA polymerase ECF-type sigma factor
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              • AAN85485.1
              		 14271 - 15254 (+) putative macrolide 2'-phosphotransferase
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                • AAN85486.1
                		 15681 - 16277 (-) putative tetR-family transcriptional regulator
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                  • AAN85487.1
                  		 16467 - 18083 (+) antibiotic efflux protein
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                    • AAN85488.1
                    		 18480 - 19382 (+) putative hydroxylase
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                      • AAN85489.1
                      		 19784 - 20377 (-) putative cyanamide hydratase
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                        • AAN85490.1
                        		 20662 - 21723 (+) histidinol-phosphate aminotransferase
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                          • AAN85491.1
                          		 21994 - 24318 (+) putative transcriptional regulator
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                            • AAN85492.1
                            		 24524 - 24742 (+) MbtH-like protein
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                              • AAN85493.1
                              		 24778 - 28095 (+) nonribosomal peptide synthetase
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                                • AAN85494.1
                                		 28128 - 29120 (+) putative regulatory protein
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                                  • AAN85495.1
                                  		 29117 - 30124 (+) putative regulatory protein
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                                    • AAN85496.1
                                    		 30172 - 31113 (+) hypothetical protein
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                                      • AAN85497.1
                                      		 31140 - 32165 (+) putative fatty acid desaturase
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                                        • AAN85498.1
                                        		 32199 - 33500 (+) diaminopimelase decarboxylase
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                                          • AAN85499.1
                                          		 33600 - 35984 (-) putative peptidase
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                                            • AAN85500.1
                                            		 36015 - 37313 (-) antibiotic transport protein
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                                              • AAN85501.1
                                              		 37317 - 40721 (-) nonribosomal peptide synthetase
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                                                • AAN85502.1
                                                		 40718 - 41548 (-) conserved hypothetical protein
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                                                  • AAN85503.1
                                                  		 41709 - 43358 (+) nonribosomal peptide synthetase
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                                                    • AAN85504.1
                                                    		 43412 - 44119 (+) thioesterase
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                                                      • AAN85505.1
                                                      		 44116 - 44946 (+) short-chain dehydrogenase/reductase
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                                                        • AAN85506.1
                                                        		 44970 - 47732 (+) nonribosomal peptide synthetase
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                                                          • AAN85507.1
                                                          		 47820 - 48407 (+) putative N-carbomoyl-sarcosine amidase
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                                                            • AAN85508.1
                                                            		 48545 - 49576 (+) hydrogenase expression/formation protein
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                                                              • AAN85509.1
                                                              		 49599 - 51974 (+) hydrogenase maturation protein
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                                                                • AAN85510.1
                                                                		 52006 - 53349 (+) serine hydroxymethyltransferase
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                                                                  • AAN85511.1
                                                                  		 53346 - 54062 (+) putative glutamine amidotransferase
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                                                                    • AAN85512.1
                                                                    		 54157 - 59394 (+) nonribosomal peptide synthetase
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                                                                      • AAN85513.1
                                                                      		 59420 - 60808 (+) nonribosomal peptide synthetase
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                                                                        • AAN85514.1
                                                                        • LnmA
                                                                        		 60948 - 62147 (+) cytochrome P450 hydroxylase
                                                                        • Tailoring
                                                                        • Knock-out
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                                                                        • AAN85515.1
                                                                        • LnmB
                                                                        		 62159 - 62395 (+) ferredoxin
                                                                        • Tailoring
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                                                                        • AAN85516.1
                                                                        • LnmC
                                                                        		 62682 - 63029 (+) hypothetical protein
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                                                                          • AAN85517.1
                                                                          • LnmD
                                                                          		 63116 - 64432 (+) putative hydrolase/decarboxylase
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                                                                            • AAN85518.1
                                                                            • LnmE
                                                                            		 64500 - 65423 (+) hypothetical protein
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                                                                              • AAN85519.1
                                                                              • LnmF
                                                                              		 65441 - 66238 (+) putative enoyl-CoA hydratase
                                                                              • Scaffold biosynthesis
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                                                                              • AAN85520.1
                                                                              • LnmG
                                                                              		 66268 - 68655 (+) trans-acting acyltransferase/oxidoreductase
                                                                              • Scaffold biosynthesis
                                                                              • Knock-out Activity assay
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                                                                              • AAN85521.1
                                                                              • LnmH
                                                                              		 68725 - 69549 (+) hypothetical protein
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                                                                                • AAN85522.1
                                                                                • LnmI
                                                                                		 69681 - 82994 (+) hybrid nonribosomal peptide synthetase / polyketide synthase
                                                                                • Scaffold biosynthesis
                                                                                • Knock-out Activity assay
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                                                                                • AAN85523.1
                                                                                • LnmJ
                                                                                		 82991 - 105040 (+) polyketide synthase
                                                                                • Scaffold biosynthesis
                                                                                • Knock-out
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                                                                                • AAN85524.1
                                                                                • LnmK
                                                                                		 105037 - 105996 (+) acyltransferase/decarboxylase
                                                                                • Scaffold biosynthesis
                                                                                • Knock-out Activity assay
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                                                                                • AAN85525.1
                                                                                • LnmL
                                                                                		 105993 - 106253 (+) acyl carrier protein type II
                                                                                • Scaffold biosynthesis
                                                                                • Knock-out Activity assay
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                                                                                • AAN85526.1
                                                                                • LnmM
                                                                                		 106250 - 107500 (+) HMG-CoA synthase
                                                                                • Scaffold biosynthesis
                                                                                • Knock-out
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                                                                                • AAN85527.1
                                                                                • LnmN
                                                                                		 107557 - 108360 (+) thioesterase type II
                                                                                • Scaffold biosynthesis
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                                                                                • AAN85528.1
                                                                                • LnmO
                                                                                		 108395 - 109078 (+) putative transcriptional activator
                                                                                • Regulation
                                                                                • Knock-out
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                                                                                • AAN85529.1
                                                                                • LnmP
                                                                                		 109122 - 109370 (+) peptidyl carrier protein type II
                                                                                • Scaffold biosynthesis
                                                                                • Knock-out Activity assay
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                                                                                • AAN85530.1
                                                                                • LnmQ
                                                                                		 109367 - 110917 (+) nonribosomal peptide synthetase, discrete adenylation domain
                                                                                • Scaffold biosynthesis
                                                                                • Knock-out Activity assay
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                                                                                • AAN85531.1
                                                                                • LnmR
                                                                                		 110973 - 112700 (-) ABC transporter component, ATP hydrolase
                                                                                • Transport
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                                                                                • AAN85532.1
                                                                                • LnmS
                                                                                		 112697 - 113560 (-) ABC transporter component, membrane spanning protein
                                                                                • Transport
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                                                                                • AAN85533.1
                                                                                • LnmT
                                                                                		 113557 - 114522 (-) ABC transporter component, membrane spanning protein
                                                                                • Transport
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                                                                                • AAN85534.1
                                                                                • LnmU
                                                                                		 114519 - 116060 (-) ABC transporter component, periplasmic oligopeptide binding protein
                                                                                • Transport
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                                                                                • AAN85535.1
                                                                                • LnmV
                                                                                		 116132 - 116494 (-) hypothetical protein
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                                                                                  • AAN85536.1
                                                                                  • LnmW
                                                                                  		 116491 - 118041 (-) 4-coumarate:CoA ligase
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                                                                                    • AAN85537.1
                                                                                    • LnmX
                                                                                    		 118049 - 118780 (-) conserved hypothetical protein
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                                                                                      • AAN85538.1
                                                                                      • LnmY
                                                                                      		 118815 - 120239 (-) antibiotic efflux protein
                                                                                      • Transport
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                                                                                      • AAN85539.1
                                                                                      • LnmZ
                                                                                      		 120436 - 121638 (-) cytochrome P450 hydroxylase
                                                                                      • Tailoring
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                                                                                      • AAN85540.1
                                                                                      • LnmZ_
                                                                                      		 121757 - 122161 (+) hypothetical protein
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                                                                                        • AAN85541.1
                                                                                        		 122182 - 122832 (-) conserved hypothetical protein
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                                                                                          • AAN85542.1
                                                                                          		 122846 - 123664 (-) thioesterase
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                                                                                            • AAN85543.1
                                                                                            		 123898 - 124935 (+) conserved hypothetical protein
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                                                                                              • AAN85544.1
                                                                                              		 124806 - 125516 (-) putative tetR-family transcriptional regulartory
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                                                                                                • AAN85545.1
                                                                                                		 125637 - 127256 (+) antibiotic resistance protein
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                                                                                                  • AAN85546.1
                                                                                                  		 127231 - 128199 (+) putative hydrolase/lactonase
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                                                                                                    • AAN85547.1
                                                                                                    		 128316 - 129971 (-) ABC transporter
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                                                                                                      • AAN85548.1
                                                                                                      		 130318 - 131727 (-) adenosylhomocysteinase
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                                                                                                        • AAN85549.1
                                                                                                        		 131705 - 132616 (-) 5,10-methylenetetrahydrofolate reductase
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                                                                                                          Biosynthesis information

                                                                                                          Biosynthetic modules

                                                                                                          Name
                                                                                                          0
                                                                                                          Type
                                                                                                          nrps-type1
                                                                                                          Genes
                                                                                                          LnmQ LnmP
                                                                                                          Substrates
                                                                                                          D-alanine (evidence: Activity assay)
                                                                                                          Integrated Monomers
                                                                                                          Domains
                                                                                                          adenylation
                                                                                                          Name
                                                                                                          1
                                                                                                          Type
                                                                                                          nrps-type1
                                                                                                          Genes
                                                                                                          LnmI
                                                                                                          Substrates
                                                                                                          cysteine (evidence: Activity assay)
                                                                                                          Integrated Monomers
                                                                                                          Domains
                                                                                                          condensation (DCL), adenylation, oxidase
                                                                                                          Name
                                                                                                          2
                                                                                                          Type
                                                                                                          pks-trans-at
                                                                                                          Genes
                                                                                                          LnmI
                                                                                                          Substrates
                                                                                                          Integrated Monomers
                                                                                                          Domains
                                                                                                          ketosynthase, ketoreductase, carrier (ACP)
                                                                                                          Name
                                                                                                          3
                                                                                                          Type
                                                                                                          pks-trans-at
                                                                                                          Genes
                                                                                                          LnmI LnmJ
                                                                                                          Substrates
                                                                                                          Integrated Monomers
                                                                                                          Domains
                                                                                                          ketosynthase, dehydratase, ketoreductase, carrier (ACP)
                                                                                                          Name
                                                                                                          4
                                                                                                          Type
                                                                                                          pks-trans-at
                                                                                                          Genes
                                                                                                          LnmJ
                                                                                                          Substrates
                                                                                                          Integrated Monomers
                                                                                                          Domains
                                                                                                          ketosynthase, carrier (ACP)
                                                                                                          Name
                                                                                                          5
                                                                                                          Type
                                                                                                          pks-trans-at
                                                                                                          Genes
                                                                                                          LnmJ
                                                                                                          Substrates
                                                                                                          Integrated Monomers
                                                                                                          Domains
                                                                                                          ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP), carrier (ACP)
                                                                                                          Name
                                                                                                          6
                                                                                                          Type
                                                                                                          pks-trans-at
                                                                                                          Genes
                                                                                                          LnmJ
                                                                                                          Substrates
                                                                                                          Integrated Monomers
                                                                                                          Domains
                                                                                                          ketosynthase, dehydratase, ketoreductase, carrier (ACP)
                                                                                                          Name
                                                                                                          7
                                                                                                          Type
                                                                                                          pks-trans-at
                                                                                                          Genes
                                                                                                          LnmJ
                                                                                                          Substrates
                                                                                                          Integrated Monomers
                                                                                                          Domains
                                                                                                          ketosynthase, carrier (ACP), carrier (ACP)
                                                                                                          Annotation changelog

                                                                                                          Entry version: 4

                                                                                                          Date
                                                                                                          Changes
                                                                                                          Submitters
                                                                                                          Reviewers
                                                                                                          2022-10-07
                                                                                                          Update chemical activity to schema version 2.11
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                                                          Entry version: 3

                                                                                                          Date
                                                                                                          Changes
                                                                                                          Submitters
                                                                                                          Reviewers
                                                                                                          2022-09-15
                                                                                                          Corrected NRP module activity
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          2022-09-15
                                                                                                          Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          2022-09-15
                                                                                                          Merged cross-CDS PKS modules
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          2022-09-15
                                                                                                          Corrected gene identifiers
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          2022-09-15
                                                                                                          Changed amino acid substrates to lower case
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                                                          Entry version: 2

                                                                                                          Date
                                                                                                          Changes
                                                                                                          Submitters
                                                                                                          Reviewers
                                                                                                          2019-10-16
                                                                                                          Migrated from v1.4
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                                                          Entry version: 1

                                                                                                          Date
                                                                                                          Changes
                                                                                                          Submitters
                                                                                                          Reviewers
                                                                                                          2015-06-12
                                                                                                          Submitted
                                                                                                          • (ID: HPZ6LL7T2WJIC44FKRYGWFQ6)
                                                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                          Detailed domain annotation
                                                                                                          Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
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