Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001101.1:
Cluster 1NRP / Polyketide1135638

BGC0001101, leinamycin biosynthetic gene cluster from Streptomyces atroolivaceus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: leinamycin
PubChem ID: 9849230
SMILES string: Copy to clipboard
Molecular formula: C22H26N2O6S3
Average molecular mass: 510.638 Da
Molecular activity: Antibacterial, Cytotoxic, Inhibitor
Molecular target: DNA

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other (cyclic)
Thioesterase type:
None
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
LnmQ (AAN85530.1)
Module 0
A specificity: D-alanine
Evidence for specificity: Activity assay
C domain subtype: N/A
LnmP (AAN85529.1)
Module 0
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: N/A
LnmI (AAN85522.1)
Module 1
A specificity: Cysteine
Evidence for specificity: Activity assay
C domain subtype: DCL
Scaffold-modifying domain: Oxidation
Polyketide subclass:
Macrolide (cyclic)
Polyketide synthase subclass:
Modular type I / Trans-AT type I
Starter unit:
Malonyl-CoA
AAN85520
Thioesterase type:
Type I
Release / cyclization type:
Macrolactamization

Modular polyketide synthases:
LnmI (AAN85522.1)
Module 2
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 3
Scaffold-modifying domain: None

LnmJ (AAN85523.1)
Module 3
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 4
Scaffold-modifying domain: None
Module 5
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module 6
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 7
Scaffold-modifying domain: None

Gene cluster description

leinamycin (BGC0001101). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank AF484556, positions 60948-122161. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Guohui Pan (The Scripps Research Institute)

Literature references

1. Cheng YQ et al. (2002) Identification and localization of the gene cluster encoding biosynthesis of the antitumor macrolactam leinamycin in Streptomyces atroolivaceus S-140. J Bacteriol 184(24):7013-24.
2. Cheng YQ et al. (2003) Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis. Proc Natl Acad Sci U S A 100(6):3149-54. doi:
3. Tang GL et al. (2004) Leinamycin biosynthesis revealing unprecedented architectural complexity for a hybrid polyketide synthase and nonribosomal peptide synthetase. Chem Biol 11(1):33-45. doi: 10.1016/j.chembiol.2003.12.014.
4. Tang GL et al. (2007) Chain initiation in the leinamycin-producing hybrid nonribosomal peptide/polyketide synthetase from Streptomyces atroolivaceus S-140. Discrete, monofunctional adenylation enzyme and peptidyl carrier protein that directly load D-alanine. J Biol Chem 282(28):20273-82. doi: 10.1074/jbc.M702814200. Epub 2007
5. Huang Y et al. (2011) Characterization of the lnmKLM genes unveiling key intermediates for beta-alkylation in leinamycin biosynthesis. Org Lett 13(3):498-501. doi: 10.1021/ol102838y. Epub 2010 Dec 30.