BGC0001101: leinamycin biosynthetic gene cluster from Streptomyces atroolivaceus
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Location: 1 - 135,638 nt. (total: 135,638 nt).
This entry is originally from NCBI GenBank AF484556.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001101
Short description leinamycin biosynthetic gene cluster from Streptomyces atroolivaceus
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Macrolide)
Loci NCBI GenBank: AF484556.1
Compounds
  • leinamycin
Species Streptomyces atroolivaceus [taxonomy]
References
Chemical products information
leinamycin [synonyms: lnm]
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C22H26N2O6S3
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAN85479.1
 3489 - 4358 (+) putative antibiotic resistance protein
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  • AAN85480.1
 5108 - 6616 (+) putative FAD-dependent oxygenase
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  • AAN85481.1
 7431 - 11144 (+) subtilisin-like secreted protease
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  • AAN85482.1
 11024 - 11812 (+) hypothetical protein
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  • AAN85483.1
 11809 - 13014 (+) putative NADH dehydrogenase II
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  • AAN85484.1
 13011 - 13931 (+) RNA polymerase ECF-type sigma factor
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  • AAN85485.1
 14271 - 15254 (+) putative macrolide 2'-phosphotransferase
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  • AAN85486.1
 15681 - 16277 (-) putative tetR-family transcriptional regulator
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  • AAN85487.1
 16467 - 18083 (+) antibiotic efflux protein
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  • AAN85488.1
 18480 - 19382 (+) putative hydroxylase
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  • AAN85489.1
 19784 - 20377 (-) putative cyanamide hydratase
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  • AAN85490.1
 20662 - 21723 (+) histidinol-phosphate aminotransferase
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  • AAN85491.1
 21994 - 24318 (+) putative transcriptional regulator
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  • AAN85492.1
 24524 - 24742 (+) MbtH-like protein
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  • AAN85493.1
 24778 - 28095 (+) nonribosomal peptide synthetase
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  • AAN85494.1
 28128 - 29120 (+) putative regulatory protein
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  • AAN85495.1
 29117 - 30124 (+) putative regulatory protein
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  • AAN85496.1
 30172 - 31113 (+) hypothetical protein
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  • AAN85497.1
 31140 - 32165 (+) putative fatty acid desaturase
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  • AAN85498.1
 32199 - 33500 (+) diaminopimelase decarboxylase
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  • AAN85499.1
 33600 - 35984 (-) putative peptidase
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  • AAN85500.1
 36015 - 37313 (-) antibiotic transport protein
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  • AAN85501.1
 37317 - 40721 (-) nonribosomal peptide synthetase
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  • AAN85502.1
 40718 - 41548 (-) conserved hypothetical protein
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  • AAN85503.1
 41709 - 43358 (+) nonribosomal peptide synthetase
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  • AAN85504.1
 43412 - 44119 (+) thioesterase
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  • AAN85505.1
 44116 - 44946 (+) short-chain dehydrogenase/reductase
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  • AAN85506.1
 44970 - 47732 (+) nonribosomal peptide synthetase
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  • AAN85507.1
 47820 - 48407 (+) putative N-carbomoyl-sarcosine amidase
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  • AAN85508.1
 48545 - 49576 (+) hydrogenase expression/formation protein
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  • AAN85509.1
 49599 - 51974 (+) hydrogenase maturation protein
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  • AAN85510.1
 52006 - 53349 (+) serine hydroxymethyltransferase
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  • AAN85511.1
 53346 - 54062 (+) putative glutamine amidotransferase
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  • AAN85512.1
 54157 - 59394 (+) nonribosomal peptide synthetase
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  • AAN85513.1
 59420 - 60808 (+) nonribosomal peptide synthetase
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  • AAN85514.1
  • LnmA
 60948 - 62147 (+) cytochrome P450 hydroxylase
  • Tailoring (Hydroxylation)
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  • AAN85515.1
  • LnmB
 62159 - 62395 (+) ferredoxin
  • Tailoring (Hydroxylation)
Sequence-based prediction
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  • AAN85516.1
  • LnmC
 62682 - 63029 (+) hypothetical protein
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  • AAN85517.1
  • LnmD
 63116 - 64432 (+) putative hydrolase/decarboxylase
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  • AAN85518.1
  • LnmE
 64500 - 65423 (+) hypothetical protein
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  • AAN85519.1
  • LnmF
 65441 - 66238 (+) putative enoyl-CoA hydratase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AAN85520.1
  • LnmG
 66268 - 68655 (+) trans-acting acyltransferase/oxidoreductase
  • Scaffold biosynthesis
Activity assay
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  • AAN85521.1
  • LnmH
 68725 - 69549 (+) hypothetical protein
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  • AAN85522.1
  • LnmI
 69681 - 82994 (+) hybrid nonribosomal peptide synthetase / polyketide synthase
  • Scaffold biosynthesis
Activity assay
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  • AAN85523.1
  • LnmJ
 82991 - 105040 (+) polyketide synthase
  • Scaffold biosynthesis
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  • AAN85524.1
  • LnmK
 105037 - 105996 (+) acyltransferase/decarboxylase
  • Scaffold biosynthesis
Activity assay
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  • AAN85525.1
  • LnmL
 105993 - 106253 (+) acyl carrier protein type II
  • Scaffold biosynthesis
Activity assay
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  • AAN85526.1
  • LnmM
 106250 - 107500 (+) HMG-CoA synthase
  • Scaffold biosynthesis
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  • AAN85527.1
  • LnmN
 107557 - 108360 (+) thioesterase type II
  • Scaffold biosynthesis
Sequence-based prediction
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  • AAN85528.1
  • LnmO
 108395 - 109078 (+) putative transcriptional activator
  • Regulation
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  • AAN85529.1
  • LnmP
 109122 - 109370 (+) peptidyl carrier protein type II
  • Scaffold biosynthesis
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  • AAN85530.1
  • LnmQ
 109367 - 110917 (+) nonribosomal peptide synthetase, discrete adenylation domain
  • Scaffold biosynthesis
Activity assay
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  • AAN85531.1
  • LnmR
 110973 - 112700 (-) ABC transporter component, ATP hydrolase
  • Transport
Sequence-based prediction
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  • AAN85532.1
  • LnmS
 112697 - 113560 (-) ABC transporter component, membrane spanning protein
  • Transport
Sequence-based prediction
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  • AAN85533.1
  • LnmT
 113557 - 114522 (-) ABC transporter component, membrane spanning protein
  • Transport
Sequence-based prediction
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  • AAN85534.1
  • LnmU
 114519 - 116060 (-) ABC transporter component, periplasmic oligopeptide binding protein
  • Transport
Sequence-based prediction
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  • AAN85535.1
  • LnmV
 116132 - 116494 (-) hypothetical protein
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  • AAN85536.1
  • LnmW
 116491 - 118041 (-) 4-coumarate:CoA ligase
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  • AAN85537.1
  • LnmX
 118049 - 118780 (-) conserved hypothetical protein
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  • AAN85538.1
  • LnmY
 118815 - 120239 (-) antibiotic efflux protein
  • Transport
Sequence-based prediction
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  • AAN85539.1
  • LnmZ
 120436 - 121638 (-) cytochrome P450 hydroxylase
  • Tailoring (Hydroxylation)
Sequence-based prediction
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  • AAN85540.1
  • LnmZ_
 121757 - 122161 (+) hypothetical protein
  • Unknown
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  • AAN85541.1
 122182 - 122832 (-) conserved hypothetical protein
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  • AAN85542.1
 122846 - 123664 (-) thioesterase
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  • AAN85543.1
 123898 - 124935 (+) conserved hypothetical protein
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  • AAN85544.1
 124806 - 125516 (-) putative tetR-family transcriptional regulartory
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  • AAN85545.1
 125637 - 127256 (+) antibiotic resistance protein
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  • AAN85546.1
 127231 - 128199 (+) putative hydrolase/lactonase
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  • AAN85547.1
 128316 - 129971 (-) ABC transporter
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  • AAN85548.1
 130318 - 131727 (-) adenosylhomocysteinase
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  • AAN85549.1
 131705 - 132616 (-) 5,10-methylenetetrahydrofolate reductase
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Polyketide-specific information
Subclass Macrolide
Starter unit Malonyl-CoA
Cyclic? yes
Release type
  • Macrolactamization
Polyketide-synthases
Genes Properties Modules
LnmG
+
LnmI
+
LnmJ 		Synthase subclass: Modular type I/Trans-AT type I
Trans-acyltansferases: LnmG
Module 2
Genes: LnmI
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 3
Genes: LnmI, LnmJ
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP), Ketoreductase
KR-domain stereochemistry: Unknown
Module 4
Genes: LnmJ
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 5
Genes: LnmJ
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 6
Genes: LnmJ
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 7
Genes: LnmJ
Core domains: Ketosynthase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
NRP-specific information
Subclass N/A
Cyclic? yes
Release type
  • Macrolactamization
Thioesterase genes
  • LnmJ (Unknown)
NRP-synthases
Gene Modules
LnmQ
Module 0
Specificity: D-alanine
Evidence for specificity:
  • Activity assay
LnmP
Module 0
LnmI
Module 1
Specificity: cysteine
Evidence for specificity:
  • Activity assay
Condensation domain type: DCL
Modification domains: Oxidation
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: HPZ6LL7T2WJIC44FKRYGWFQ6, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Merged cross-CDS PKS modules
  • Corrected gene identifiers
  • Changed amino acid substrates to lower case
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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