BGC0001102: malleilactone biosynthetic gene cluster from Burkholderia thailandensis E264; ATCC 700388
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Location: 2,543,245 - 2,580,940 nt. (total: 37,696 nt).
This entry is originally from NCBI GenBank CP000085.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001102
Short description malleilactone biosynthetic gene cluster from Burkholderia thailandensis E264; ATCC 700388
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "Putative gene functions: BurR: LuxR-type regulator, BurA: Gonyol-Synthetase (NRPS/PKS), BurB: methyltransferase, BurC: hydroxylase, BurD: aminotransferase, BurE: dehydrogenase, BurF: NRPS/PKS, BurG: reductoisomerase, BurH: FkbH-like protein, BurI: decarboxylase, BurJ: acyl-CoA ligase, BurK: unknown, BurL: acyltransferase, BurM: acyl-CoA ligase, BurN: efflux carrier protein"
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
CP000085.1
2543245 - 2580940
via Knock-out studies, Enzymatic assays, Heterologous expression, Gene expression correlated with compound production
Compounds
  • malleilactone
  • bis-malleicyprol
  • malleicyprol
Species Burkholderia thailandensis E264; ATCC 700388 [taxonomy]
References
Chemical products information
malleilactone [synonyms: burkholderic acid] Evidence:
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C18H26O4
Chemical database entries
PubCHEM
NPAtlas
bis-malleicyprol Evidence:
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C36H52O8
malleicyprol Evidence:
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C18H26O4
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • BTH_II2087
  • ABC34352.1
 2543245 - 2544522 (-) LuxR-type regulator
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    • BTH_II2088
    • ABC36203.1
    		 2545193 - 2553571 (+) thiotemplate mechanism natural product synthetase
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      • BTH_II2089
      • ABC34581.1
      		 2553671 - 2554189 (+) conserved hypothetical protein
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        • BTH_II2090
        • ABC35239.1
        		 2554346 - 2555740 (+) syringomycin synthesis regulator SyrP, putative
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          • BTH_II2091
          • ABC35627.1
          		 2555749 - 2557170 (+) adenosylmethionine-8-amino-7-oxononanoate aminotransferase, putative
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            • BTH_II2092
            • ABC35115.1
            		 2557164 - 2558576 (+) aldehyde dehydrogenase family protein
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              • BTH_II2093
              • ABC35796.1
              		 2558633 - 2570779 (+) polyketide synthase, putative
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                • BTH_II2094
                • ABC34926.1
                • ilvC-2
                		 2570785 - 2571846 (+) NAD+-dependent ketol-acid reductoisomerase
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                  • BTH_II2095
                  • ABC34723.1
                  		 2571743 - 2574466 (+) diaminopimelate decarboxylase, putative
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                    • BTH_II2096
                    • ABC34346.1
                    		 2574496 - 2576364 (+) long-chain-fatty-acid--CoA ligase, putative
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                      • BTH_II2097
                      • ABC34855.1
                      		 2576437 - 2576868 (+) lipoprotein, putative
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                        • BTH_II2098
                        • ABC35574.1
                        • fabD-2
                        		 2576869 - 2577768 (+) malonyl CoA-acyl carrier protein transacylase
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                          • BTH_II2099
                          • ABC35922.1
                          		 2577785 - 2579653 (+) AMP-binding domain protein
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                            • BTH_II2100
                            • ABC35961.1
                            		 2580041 - 2580940 (+) auxin efflux carrier, putative
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                              Biosynthesis information

                              Biosynthetic modules

                              Name
                              3
                              Type
                              nrps-type1
                              Genes
                              BTH_II2088
                              Substrates
                              propionic acid (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              adenylation
                              Name
                              1
                              Type
                              pks-trans-at
                              Genes
                              BTH_II2093
                              Substrates
                              Integrated Monomers
                              Domains
                              ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP), carrier (ACP)
                              Name
                              2
                              Type
                              pks-trans-at
                              Genes
                              BTH_II2093
                              Substrates
                              Integrated Monomers
                              Domains
                              ketosynthase, carrier (ACP)
                              Name
                              4
                              Type
                              pks-modular
                              Genes
                              BTH_II2088
                              Substrates
                              Integrated Monomers
                              Domains
                              acyltransferase, ketosynthase, acyltransferase, thioesterase, ketoreductase
                              Annotation changelog

                              Entry version: next

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2024-03-19
                              MIBiG v4 annotathon
                              • (ID: WM5BFBJLPUJPB7YQHVARW7DU)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 4

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-10-07
                              Update chemical activity to schema version 2.11
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 3

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-09-15
                              Corrected NRP module activity
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Merged burkholderic acid annotation
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Updated bioactivity data
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Updated NRP substrate specificities
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 2

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2019-10-16
                              Migrated from v1.4
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2019-10-16
                              Updated compound(s) information (NPAtlas curation)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 1

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2015-06-12
                              Submitted
                              • (ID: WBX64IC5EUFODOV2ECKGLTGC)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              Detailed domain annotation
                              Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
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