BGC0001125 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001125 (Cylindrospermum alatosporum CCALA 988)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	56,729	High	puwainaphycin F/minutissamide A	NRPS:Type I

BGC0001125 - Region 1 - NRPS,T1PKS

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Location: 1 - 56,729 nt. (total: 56,729 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 56,730
Label		BGC0001125

Tailoring functions

Ligases: ligase synthase

puwA: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

puwB: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

puwC: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

puwE: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

puwF: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

puwG: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

puwH: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001125.5	1.80	NRPS, PKS	puwainaphycin A, puwainaphycin B, puwainaphycin C, puwainaphycin D	Cylindrospermum alatosporum CCALA 988
BGC0001951.4	1.16	NRPS	puwainaphycin F, minutissamide A	Cylindrospermum alatosporum CCALA 994
BGC0001950.4	1.16	NRPS	puwainaphycin F, minutissamide A	Cylindrospermum moravicum CCALA 993
BGC0001758.4	0.98	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002135.2	0.97	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.95	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000342.4	0.92	NRPS	enniatin	Fusarium equiseti
BGC0001128.4	0.92	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.91	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.91	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001125.5	0.98	NRPS, PKS	puwainaphycin A, puwainaphycin B, puwainaphycin C, puwainaphycin D	Cylindrospermum alatosporum CCALA 988
BGC0001951.4	0.84	NRPS	puwainaphycin F, minutissamide A	Cylindrospermum alatosporum CCALA 994
BGC0001950.4	0.83	NRPS	puwainaphycin F, minutissamide A	Cylindrospermum moravicum CCALA 993
BGC0001758.4	0.78	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002135.2	0.78	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.77	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000342.4	0.76	NRPS	enniatin	Fusarium equiseti
BGC0001128.4	0.76	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.76	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 56729:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (Asn - D-Ala) + (Thr - X - Thr) + (mal - Val) + (Me-redmal) + (NMe-Asn - Pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

puwA: Asn - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (513..915): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code(s):: Asn DATKVGEVGK (100% 8Å match); OH-Asn or Asn DATKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1996..2401): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFMAQVVK
Nearest Stachelhaus code(s):: Pro DVQFMAQVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

puwB: mal

PKS_AT (540..838): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.4
inactive: 167.3
Methoxymalonyl-CoA: 67.5
Methylmalonyl-CoA: 58.3
Isobutyryl-CoA: 37.5
2-Methylbutyryl-CoA: 33.1
Propionyl-CoA: 32.2
Benzoyl-CoA: 26.2
Ethylmalonyl-CoA: 24.4
Acetyl-CoA: 22.0
trans-1,2-CPDA: 12.2
CHC-CoA: 11.8
3-Methylbutyryl-CoA: 5.3
fatty_acid: 0.0

puwC: NH2

CAL_domain (62..506): NH2

minowa: NH2

Prediction, score:

NH2: 202.8
fatty_acid: 8.7
AHBA: 7.9
Acetyl-CoA: 3.0
shikimic_acid: 0.0

puwE: mal - Val

Search NORINE for peptide: strict or relaxed

PKS_AT (570..866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.0
inactive: 178.9
Methoxymalonyl-CoA: 78.5
Methylmalonyl-CoA: 76.9
Propionyl-CoA: 60.1
2-Methylbutyryl-CoA: 51.1
Isobutyryl-CoA: 43.6
Acetyl-CoA: 36.7
Ethylmalonyl-CoA: 36.4
CHC-CoA: 25.6
Benzoyl-CoA: 24.8
fatty_acid: 20.2
trans-1,2-CPDA: 18.2
3-Methylbutyryl-CoA: 13.2

AMP-binding (2480..2877): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

puwF: Thr - X

Search NORINE for peptide: strict or relaxed

AMP-binding (540..948): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1613..2013): X

nrpys: Gln, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code(s):: Gln or Asn DATKVGEVGK (100% 8Å match); Asn DATKVGEVGK (88% 8Å match); OH-Asn or Asn DATKVGEVGK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

puwG: Thr - Asn - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (283..691): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1355..1785): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code(s):: Asn DATKVGEVGK (100% 8Å match); OH-Asn or Asn DATKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2442..2835): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match); Gly DLFNNALTYK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

puwH: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (478..886): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor