BGC0001143 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001143 (Aspergillus niger ATCC 1015)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	67,810	High	azanigerone A/azanigerone B/azanigerone C/azanigerone D/azanigerone E/azanigerone F	PKS

BGC0001143 - Region 1 - T1PKS

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Location: 1 - 67,810 nt. (total: 67,810 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 67,811
Label		BGC0001143

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxygenase

ASPNIDRAFT_132654: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

ASPNIDRAFT_189194: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

ASPNIDRAFT_43447: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ASPNIDRAFT_43449: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Lyases: dehydratase

ASPNIDRAFT_212676: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

ASPNIDRAFT_188806: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ASPNIDRAFT_188817: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ASPNIDRAFT_56946: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001143.4	0.99	PKS	azanigerone A, azanigerone B, azanigerone C, azanigerone D, azanigerone E, azanigerone F	Aspergillus niger ATCC 1015
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001268.4	0.65	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000037.4	0.64	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001143.4	0.99	PKS	azanigerone A, azanigerone B, azanigerone C, azanigerone D, azanigerone E, azanigerone F	Aspergillus niger ATCC 1015
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001268.4	0.65	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000037.4	0.64	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 67810:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (Me-redmal)
Non-colinear ordering:: ASPNIDRAFT_56946 + ASPNIDRAFT_188817

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ASPNIDRAFT_188806: X

AMP-binding (21..441): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AAPTRGVCIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ASPNIDRAFT_56946: pk

PKS_AT (900..1196): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Ethylmalonyl-CoA: 54.2%; inactive: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 60.7
Methylmalonyl-CoA: 49.7
Malonyl-CoA: 49.2
inactive: 44.2
trans-1,2-CPDA: 22.5
Propionyl-CoA: 22.4
Ethylmalonyl-CoA: 22.2
CHC-CoA: 21.8
Isobutyryl-CoA: 17.0
2-Methylbutyryl-CoA: 16.5
Benzoyl-CoA: 14.2
3-Methylbutyryl-CoA: 13.6
Acetyl-CoA: 11.0
fatty_acid: 0.0

ASPNIDRAFT_188817: mal

PKS_AT (554..876): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 90.9
Methylmalonyl-CoA: 66.1
Methoxymalonyl-CoA: 65.2
Isobutyryl-CoA: 29.8
Benzoyl-CoA: 22.3
inactive: 22.1
trans-1,2-CPDA: 17.0
fatty_acid: 16.3
CHC-CoA: 16.2
Propionyl-CoA: 15.7
2-Methylbutyryl-CoA: 14.7
Ethylmalonyl-CoA: 5.9
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor