LOCUS       BGC0001146             12498 bp    DNA     linear   BCT 06-OCT-2014
DEFINITION  Streptomyces sp. NRRL WC-3908 thiazole synthase (ctmI),
            dehydrogenase (ctmB), precursor peptide (ctmA), cyclodehydratase
            (ctmD), dehydratase (ctmE), dehydratase (ctmF), and LuxR regulatory
            protein (luxR) genes, complete cds.
ACCESSION   BGC0001146
VERSION     BGC0001146
KEYWORDS    .
SOURCE      Streptomyces sp. NRRL WC-3908
  ORGANISM  Streptomyces sp. NRRL WC-3908
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 23:27:35
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12498
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..1899
                     /gene="ctmI"
     CDS             1..1899
                     /codon_start=1
                     /gene="ctmI"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Heterocycloanthracin_C_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Thiopeptide_F_RRE"
                     /gene_kind="biosynthetic"
                     /product="thiazole synthase"
                     /protein_id="AID54691.1"
                     /sec_met_domain="Thiopeptide_F_RRE (E-value: 3.5e-30,
                     bitscore: 92.9, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Heterocycloanthracin_C_RRE (E-value:
                     2.4e-15, bitscore: 45.2, seeds: 5, tool:
                     rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPKLKPDVLYVPTADGVHLFGAGANLELRGGAIYQWVDRLAPHL
                     NGTVTLDNLLRGLPDDKRAVVAALLERLEDGGCLTDAEADEPHGLTDRELATYAPEIAF
                     VEYYRGSAARRFETYRNSSVVVLGAGPAFTALIASALHSGVRRLRAVCTPEFPTDEDRL
                     TELTGGSERRDPDQELIRTVLSGDSDAELAAALEGADAVLHVSSAPVTDRAVRLHRLCE
                     RADRLLVQGIVLDDVAWLGPSGAAWASAWQRLAAPEPSRPSAFLTGPTAAVVAGHLGLA
                     CFTALTGARDDGPPTVTRIDLETLRTSTHPFLPHPSAVDAPQESAEEFGQRVERLRSAE
                     PLLPADFSARAAACFDRYTGVLRALEEGDFTQLPLNVTEAVRPEGTGERVFGWGTGFAE
                     ARQRAALRGLARYAVTAPDPRRFADDGSVRGWDLARKEVTTVPAGDVFTSGPGVTAALD
                     WTGAVTEAVAQQCAWLAVEDVRAGAAEPVLLDVAGASLDEAGRRHLDMLRPAGGEVTAA
                     DIGTALGVPVLAWWQGARPVGVTCGPGAVTDGLELVLLDRQSELTGESAYAPQPVPGLG
                     APPRGTDGAAVPADADLPALVEALASRGRSPVVVPLDHDPAVHEVLPNVVRVVLAG"
     source          1..12498
                     /db_xref="taxon:1509516"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. NRRL WC-3908"
                     /strain="NRRL WC-3908"
     protocluster    1..12498
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3900:9533]"
                     /cutoff="20000"
                     /detection_rule="((YcaO or TIGR03604) and ((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or PF07366 or PF06968 or PF04055) or
                     thiostrepton)"
                     /neighbourhood="10000"
                     /product="thiopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3901..9533
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((YcaO or TIGR03604) and ((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or PF07366 or PF06968 or PF04055) or
                     thiostrepton)"
                     /neighbourhood="10000"
                     /product="thiopeptide"
                     /protocluster_number="1"
     protocluster    1..12498
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1895:5853]"
                     /cutoff="20000"
                     /detection_rule="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /neighbourhood="10000"
                     /product="LAP"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      1896..5853
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /neighbourhood="10000"
                     /product="LAP"
                     /protocluster_number="2"
     cand_cluster    1..12498
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /detection_rules="((YcaO or TIGR03604) and ((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or PF07366 or PF06968 or PF04055) or
                     thiostrepton)"
                     /kind="chemical_hybrid"
                     /product="LAP"
                     /product="thiopeptide"
                     /protoclusters="2"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..12498
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001146"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12498
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="LAP"
                     /product="thiopeptide"
                     /region_number="1"
                     /rules="((((goadsporin_like or PF00881 or TIGR03605) and
                     (YcaO or TIGR03604)) or TIGR03882 or TIGR03603 or
                     TIGR03602) and not cyanobactin_synth)"
                     /rules="((YcaO or TIGR03604) and ((thio_amide and (PF06968
                     or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N
                     or PF07366 or PF06968 or PF04055) or thiostrepton)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1896..3479
                     /gene="ctmB"
     CDS             1896..3479
                     /codon_start=1
                     /gene="ctmB"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     PF00881"
                     /gene_kind="biosynthetic"
                     /product="dehydrogenase"
                     /protein_id="AID54692.1"
                     /sec_met_domain="PF00881 (E-value: 1.8e-12, bitscore: 36.5,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGTATDEAGALGLRFWYDTFEGVRELFAEGHGSEGGPDDPLPSK
                     TYRGLPRHVLAAPPRRVGDARWSFPGFRARHPEGPPRRPWDGSTLSALLHHTYGLGRME
                     LGPQAAWPYHRLVASARCFYPVELYVWLDGDTGDVPAGCYHYDPLHHALTALRPGGVPE
                     ELRTAVACEGRDAAALVLLSAYFRKTAFRYRDYAYRLCAQESGMVAGNALLVAGALGLD
                     ARVHHRFLDEPVNRALGLDGTEETTFAVLEIGARDGVAAAPARDLTPLVTEHVRTNRED
                     AARWTRLTTLDAGSRLTGEARFAPVAPGPFHQPADGPAEPFADDERPPGSVELGDALRR
                     RDSGGRMFQPDRAALPQQQLADLLRYALDPVPSDHGGTPLRPLLDCHVLALNTTGRTAG
                     LYRLDPSPALVPVPGRDGRPTVAMLCDRTPSVNTARANAVVFLTVDRYEGARRFGDRGY
                     RVLHHEGGIVAQRLSVLAAAEGLAARITNGYDDRVVRELLGCGDAHVPIFAIVLGRRQA
                     TSQYEPPLVW"
     gene            3625..3807
                     /gene="ctmA"
     CDS             3625..3807
                     /codon_start=1
                     /gene="ctmA"
                     /gene_functions="biosynthetic-additional (thiopeptides)
                     predicted thiopeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="precursor peptide"
                     /protein_id="AID54693.1"
                     /transl_table=11
                     /translation="MDANLVLDLDDLSVDQLDILPTAPGSTLESINVGHAMVEIGASNC
                     TSKGSPASCCSCCCC"
     CDS_motif       3625..3750
                     /prepeptide="leader"
                     /note="peptide class: thiopeptide"
                     /note="predicted sequence:
                     MDANLVLDLDDLSVDQLDILPTAPGSTLESINVGHAMVEIGA"
                     /locus_tag="ctmA_thiopeptide"
                     /aSTool="thiopeptides"
                     /tool="antismash"
     CDS_motif       3751..3807
                     /prepeptide="core"
                     /locus_tag="ctmA_thiopeptide"
                     /peptide="thiopeptide"
                     /predicted_class="Type III"
                     /score="23.60"
                     /molecular_weight="1744.0"
                     /monoisotopic_mass="1742.5"
                     /core_sequence="SNCTSKGSPASCCSCCCC"
                     /aSTool="thiopeptides"
                     /tool="antismash"
                     /leader_sequence="MDANLVLDLDDLSVDQLDILPTAPGSTLESINVGHAMVEIG
                     A"
                     /leader_location="[3624:3750](+)"
                     /alternative_weights="1762.0"
                     /alternative_weights="1780.1"
                     /alternative_weights="1798.1"
                     /alternative_weights="1816.1"
                     /alternative_weights="1834.1"
                     /alternative_weights="1852.1"
     gene            3901..5853
                     /gene="ctmD"
     CDS             3901..5853
                     /codon_start=1
                     /gene="ctmD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     YcaO"
                     /gene_kind="biosynthetic"
                     /product="cyclodehydratase"
                     /protein_id="AID54694.1"
                     /sec_met_domain="TIGR03604 (E-value: 1.3e-110, bitscore:
                     358.6, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 7.3e-78, bitscore: 251.4,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQQGNVADRPAGLAVRGDGLLAQAVRAAVSRRHPSAAEDSDRCAA
                     LLTVDDGWQDADRTSRPDLPWLPVRVELGNVVIGPLELPGRPGCAQCARTRRQRALGQD
                     SPRAELLRRHGDRLAERPSPLLSAWARDTVAAFVEAEAAAVLDDPERAGTRNAITIIGL
                     AGLTADSHPFLPDPCCPRCGSLPDDGPPILMDTPQPKLDRDTYRVRSLDTGWDGLLRTY
                     VDGHTGIVQQLDVRTDYAYPMVSAPLRLPDGIQEAGFGRGLDYTGCERTALAEALERLG
                     GARPGGRRTAVRAGYEQVADRAVRPTDLGLYPPDRYADPEFPYPAFTPDLEINWVWGHS
                     FGRGGPVLVPEDYAYYRTRHGNDTARPLAYEVSNGCALGGCLEEAALHGILELAERDAF
                     LLTWYARLPVPRVDLDTVADPAVRLLVERIRQDTGHRVAAFATTPEHGIATSWVMAVHP
                     GDAVEPAAICAAGAHFDPEKALLNGLLELTANLGWNRAAYREELRRIEGMVADPHQVRE
                     MRDHALLYCHPDAYDRFAFLPGEETARPVAEAFARATCRPRHADLRDDLAEVVERFTAR
                     GLDVIVVDQTTDEHRAAGFACAKVIIPGLLPMTFGHRMSRTHGLPRLYDLPARLGYRDG
                     PLEPADVNPHPHPFP"
     gene            5939..8533
                     /gene="ctmE"
     CDS             5939..8533
                     /codon_start=1
                     /gene="ctmE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     75.7; E-value: 3.1e-23)"
                     /gene_kind="biosynthetic"
                     /product="dehydratase"
                     /protein_id="AID54695.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 3.4e-87, bitscore:
                     281.9, seeds: 73, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGFHVADTFGVRIGGLPVSVLDGLRSDEVWRRTGALLDGARELA
                     DRGAELSDALYALIGQETGVKAALVALRRAVHNQRTPGERCWNDEVRAALPAEVVADVE
                     RWAELLAAHRAGTAALDGLLPQDAADRRAALREAAAQPVFRHGLIQGSPVLHEQLRKWL
                     ARPEGAVPDRKLALRLAKYLARVAAKTSPFSTFTVSGLGRWDGPRPDGDGPAVRDTVEI
                     NLWLVQQLVRRLREHPALAPAVRLRVNPSAHQADGHWEFLGPGEEEPLRTLPVSPPVQA
                     CVDFLADGARPRHEVAAHLTERSGASPEQAERYVARLTELGVLETVPPFSEQAMDPLAE
                     LRAWVDTGGPELTEVSARLKEVAALLDGYPELTDPDARRRRTEEIHRALGRVADLVGPE
                     QVIVPPKNYIIENALLVGPPPAQDRTAWEPVLRDLDTVRRCYAALDPALPGRVALARLF
                     TTRFGSGASVPFLAFHRAVQEEMRTDPQLPGLLSVSQHGYAPLADSPLPRIKELLRVRA
                     GLTGAALNARPDADGVVHVDPARLAALADSWPAWMRPPGSVAFYGQLTGGSEDPAGFVV
                     NAVNAGHGRGRDRIRRLLGQAGVTVAGEPSGPPGDVILADTCRHYGSNVGLRDLTMTDE
                     LDYPGSASPRPAERRIPLRDLEVRHDPALDLLVLWSRTRDREVRPVHPSLIAEFWLPPA
                     MRLLVQAFGDTPTLLIPGRRMFGDISPATAQGLLHEPRITIGTVTVSRRQWVFRTDEAP
                     VRAKGEADRPYLLRLAAWLREHGIPERCFVRALDPSMLTDGNVWQLKSRKPLYIDFANL
                     LLTGLFERMLAERGNLLFLQEALPDPSAAPHYGDAGARVTEHLVEVGHHGRC"
     gene            8520..9533
                     /gene="ctmF"
     CDS             8520..9533
                     /codon_start=1
                     /gene="ctmF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: Lant_dehydr_C"
                     /gene_kind="biosynthetic"
                     /product="dehydratase"
                     /protein_id="AID54696.1"
                     /sec_met_domain="Lant_dehydr_C (E-value: 3.6e-70, bitscore:
                     225.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAADRPLDPATAGASDWVCAHVFQDTGHDALLTGCLRPLAKELT
                     ADGLLHRYFFLRYWEGGPHVRMRFLPTGTAASREVRQRVVTAVGAFLRDHPAPDTVDRT
                     RYAGLAGELAALEDLSEHDRELRPNNTVEFLPYLREHAVYGHGPAMAATEQHFHESSEL
                     ALALIAAGTTGQQRAAIALDLMLAVLALCDELRAQWVDSGQPLVPFAEGSEPAATEEHY
                     LARREHLRERALRVWQLAPDPTGATPQAHWLRSLRTLRDRLSGWEDAGTFTSEWARSPL
                     NQGPDLAALPHPATTLILLRCAHLANNRLGLTLSEENHLRFLAGRIVTDLPSLPVR"
     gene            complement(9607..12498)
                     /gene="luxR"
     CDS             complement(9607..12498)
                     /codon_start=1
                     /gene="luxR"
                     /gene_functions="regulatory (smcogs) SMCOG1149:LuxR family
                     transcriptional regulator (Score: 502; E-value: 6.1e-152)"
                     /gene_kind="regulatory"
                     /product="LuxR regulatory protein"
                     /protein_id="AID54697.1"
                     /transl_table=11
                     /translation="MPLTGREAELARLDGLIGRAGGGVPVIVAVRGERGVGKSALLEAV
                     AARAADAGFRVGTAAGGPAVRDSLFAAARQALGRPDDGTDTLDGHVARGRDVMARTPLA
                     LCVDDLMDVDASSATWLVALASLDRPVPLLIVAAAGDDPGSGRTAGAAELLARAEHLEL
                     TGLPEAGIAEFAARHCGLALDAAAAAVCHELTGGNPGLLLAHLAGRPGPAVTARELRGA
                     AGPEALRCTRRWLDDLPRPALAMARAVAVLGDSAEVAHAAALAELPVPDALHAIDTLVA
                     RQVLANRVPPAFRHPLLGTAVLAGMPVGSRIAAHLKVARFLRDEQWGAERVAQQLAAAG
                     PTGLDWAVEPLRAAARKLLHEGRAEEASRYLRCVLRDRLPADVRSAVRRELAEAETFVD
                     PDLALSELDTLLRESEDPEVCAELALDVAGMLIDRARPADAASVLDETAERIGAGHPAA
                     VRRLTLRKASVSLLDAAGPSAEARRLADADAAEPSGGGRSRELANLRAAIAADRGTDRD
                     AALAPAREALTGAEGGDLGRLWQSCATLIRADELVEARAHCWRTGRLSRAMSGRRAGLA
                     ADIFRARICRVAGDLPGAVAILRPVVEEIGPGSAALPMVAGFAVAGLVEALAQLGDQDE
                     AAALLAAHGVAGHGPDDDLHARYEGPYLLAARAALWRLRGEPGRAAVDLLAAGRQLVRR
                     GAVSPAVLPWRSRAALALAADGATDRAETLAREELEAARRWGTPRSIGVARHALAMTRS
                     GERRLMLLTEAVRHLEASPARLELAVARADLGAALRAAGRTAEADRECAAALALAGECG
                     ATPLARRLRAAPEPVPAHDKPVRTHPLLTPQELKIAGLARSGHTNRQISQMLFLTMRTV
                     EFHLSGVYRKLGIAGRQGLAAAIPAEPSAEPPEDQARPAAQPADGRPRHTGPAPCSCSR
                     ALPPGQPCADTPVRPSRWHDGPRSAELRPSPSR"
     misc_feature    complement(12490..12492)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 atgcgaccga aactcaagcc cgacgtgctg tacgtgccca ccgcggacgg ggtccacctg
       61 ttcggcgcgg gcgccaacct ggaactgcgg ggcggcgcca tctaccagtg ggtggaccgg
      121 ctggcgccgc acctgaacgg caccgtgacc ctggacaacc tgctgcgcgg cctccccgac
      181 gacaagcggg ccgtggtcgc ggccctgctg gagcgcctgg aggacggcgg ctgcctgacc
      241 gacgcggaag cggacgagcc gcacggcctg acggaccgcg aactggcgac gtacgcgccg
      301 gagatcgcct tcgtggagta ctaccgcggt tcggcggccc ggcgcttcga gacgtaccgc
      361 aacagctcgg tggtggtgct gggcgccggg cccgcgttca ccgcgctgat cgcttcggcg
      421 ctgcactcgg gcgtccggcg cctgcgtgcc gtgtgcaccc ccgagttccc caccgacgag
      481 gaccggctga ccgaactgac cggcgggtcc gagcggcgcg acccggacca ggagctgatc
      541 cggacggtgc tgtccggcga ctccgacgcg gaactcgcgg cggcgctgga gggcgccgac
      601 gccgtcctgc acgtgtcctc ggcgccggtg acggaccggg ccgtacggct gcaccggctg
      661 tgcgagcggg ccgaccggct gctggtccag ggcatcgtgc tggacgacgt ggcgtggctg
      721 gggccgtccg gcgcggcctg ggcgtcggcc tggcagcgcc tcgcggcgcc ggagccgtcg
      781 cggcccagcg ccttcctcac cgggccgacc gcggcggtgg tcgccggtca tctgggcctg
      841 gcctgtttca ccgcgctgac gggcgcccgg gacgacgggc cgccgacggt gacccggatc
      901 gacctggaga ccctgcgcac ttcgacccac cccttcctcc cccacccgtc ggcggtggac
      961 gcgccgcagg agagcgccga ggagttcggg cagcgcgtcg aacgcctccg ctccgccgaa
     1021 ccgctgctcc cggcggactt ctccgcgcgc gccgcggcct gcttcgaccg gtacacggga
     1081 gtgctgcgag cgctggagga aggcgacttc acgcagttgc cgctgaacgt gaccgaggcg
     1141 gtacggcccg aagggaccgg cgagcgggtc ttcggctggg gcaccggctt cgccgaggcc
     1201 cggcagcggg cggcgctgcg cgggctggcc cggtacgcgg tgacggcgcc cgatccgcgg
     1261 cgcttcgccg acgacgggtc ggtacggggc tgggacctcg cccgcaagga ggtcacgacg
     1321 gtgccggccg gggacgtgtt cacgtcgggg cccggcgtga ccgccgcact cgactggacc
     1381 ggggcggtca ccgaggcggt cgcgcagcag tgtgcgtggc tggcggtgga ggacgtacgg
     1441 gcgggcgcgg ccgaaccggt cctgctggac gtggcgggcg cctcgctgga cgaggcgggc
     1501 cgccgccacc tggacatgct gcgtccggcg ggcggcgagg tcacggccgc cgacatcggt
     1561 acggcactcg gtgtgccggt gctggcctgg tggcagggcg cccggccggt cggagtgacc
     1621 tgcggccccg gcgcggtcac cgacggcctc gaactggtcc tgctggaccg gcagtccgag
     1681 ctgaccggcg agtccgcgta cgctccgcag ccggtccccg gactcggcgc gccgccgcgc
     1741 gggacggacg gcgctgccgt accggcggac gcggacctgc ccgcactggt ggaggcgctg
     1801 gcctcgcggg gccggtcccc cgtcgtggtg ccgctcgacc acgacccggc cgtgcacgag
     1861 gtgctgccga acgtcgtacg ggtggtgctc gccggatgac cgggaccgcc accgacgagg
     1921 ccggagcgct gggactgcgg ttctggtacg acaccttcga gggcgtacgg gagctgttcg
     1981 ccgaggggca cggttccgag ggcgggcccg acgacccgct gccgagcaaa acctaccggg
     2041 ggctgccccg ccacgtactg gccgccccgc cccggcgcgt cggcgacgcg cgctggtcgt
     2101 tccccggctt ccgggcccgc caccccgaag gcccgccgcg caggccctgg gacgggagca
     2161 cactgtccgc gctgctgcac cacacctacg gtctcggccg gatggagctg ggcccgcagg
     2221 ccgcgtggcc gtaccaccgg ctggtggcct cggcccgctg cttctacccg gtggaactgt
     2281 acgtgtggct ggacggcgac accggggacg taccggcggg gtgctaccac tacgacccgc
     2341 tccaccacgc gctgaccgcg ctgcggcccg gcggcgtccc cgaggaactg cggacggcgg
     2401 tggcctgcga gggccgggac gcggccgcgc tggtgctgct ctccgcgtac ttccgcaaga
     2461 ccgccttccg gtaccgcgac tacgcctacc ggctgtgcgc gcaggagtcc ggcatggtcg
     2521 ccggcaacgc cctgctggtg gccggtgcgc tcggcctgga cgcccgggtc caccaccggt
     2581 tcctggacga gccggtgaac cgggcgctgg gcctggacgg cacggaggag accacgttcg
     2641 ccgtgctgga gatcggggcg cgggacgggg tggccgcggc tccggcgcgg gacctgacgc
     2701 ccctcgtgac ggagcacgtc cgtaccaacc gggaggacgc ggcgcgctgg acccggctga
     2761 ccaccctgga cgccggcagc cggctgaccg gtgaggcgcg cttcgcgccg gtcgccccgg
     2821 gcccgttcca ccagcccgcc gacggccccg cggagccctt cgccgacgac gagcggccgc
     2881 cggggagcgt cgagttgggc gacgcgctgc ggcgccggga ctcgggcggg cgaatgttcc
     2941 agccggaccg tgccgcgctg ccccagcagc agctcgcgga cctcctgcgg tacgcgctgg
     3001 acccggtgcc ctccgaccac ggcggcacac cgctgcggcc gctgctggac tgccacgtac
     3061 tggccctcaa caccacaggc cggaccgcag gtctgtaccg gctggacccg tcccccgcgc
     3121 tcgtccccgt acccggacgc gacgggcggc ccacggtggc catgctgtgc gaccgcacgc
     3181 cctcggtgaa caccgcgcgc gccaacgcgg tcgtcttcct caccgtggac cgctacgagg
     3241 gcgcccgccg gttcggggac cgcggctacc gcgtcctgca ccacgagggc ggcatcgtgg
     3301 cccagcggct gagcgtactg gccgccgccg agggcctcgc ggcccggatc accaacgggt
     3361 acgacgaccg ggtcgtacgg gagctgctgg gctgcgggga cgcgcacgta ccgatcttcg
     3421 cgatcgtgct gggccggcgg caggccacct cccagtacga acccccgctg gtctggtgac
     3481 cacaggcacc gtaggccacg ggcccacggc agaccctacg ggtgctccgt agagacccgg
     3541 ggccccagcg gatcacagaa tcagctcgga cagcgggcga ccgccgcccg cagtcgtcac
     3601 ccacagagga aggagggaca cgacatggac gcgaacctgg tgctggacct cgacgacctg
     3661 agcgtggacc agctcgacat tctcccgacc gcccccggct ccaccctgga gtcgatcaac
     3721 gtcggccacg ccatggtcga gatcggcgct tcgaactgca cctcgaaggg ctcgcccgcg
     3781 agctgctgct cttgttgctg ctgctgatca cgctgcacaa cagggccgtg tccggcgcgt
     3841 tccgcgcgtc gggcacggcc cggtgccact gacgggacag gaagacggga cgtgcgaagg
     3901 atgcagcagg ggaacgtggc ggaccggccg gccggactcg cggtacgggg cgacgggctg
     3961 ctcgcccagg cggtccgcgc ggcggtctcc cgccggcacc cgtcggccgc cgaggacagc
     4021 gaccggtgcg ccgcactgct caccgtggac gacggctggc aggacgccga ccggacgagc
     4081 cggccggatc tgccgtggct gcccgtccgg gtggagctgg gcaatgtcgt catcgggccg
     4141 ctcgaactgc cgggccggcc gggatgcgcg cagtgcgccc gtacccgcag gcagcgggcc
     4201 ctgggacagg acagcccccg cgccgaactg ctgcgccggc acggcgaccg gctcgccgaa
     4261 cggccgtccc cgctgctgtc cgcctgggcg cgcgacaccg tcgcggcctt cgtggaggcg
     4321 gaagccgccg ccgtgctcga cgacccggag cgcgccggca cccgcaacgc gatcaccatc
     4381 atcggtctgg ccggcctcac cgccgactcc cacccgttcc tgcccgaccc ctgctgcccc
     4441 cgctgcggca gcctccccga cgacgggccg ccgatcctga tggacacccc gcagcccaag
     4501 ctcgaccggg acacctaccg cgtccgcagc ctcgacaccg gctgggacgg tctgctgcgg
     4561 acgtacgtgg acggacacac cggcatcgtc cagcagctcg acgtacggac cgactacgcc
     4621 taccccatgg tctccgcgcc gctgcggctg cccgacggca tccaggaggc gggcttcggc
     4681 cgcggcctgg actacaccgg gtgcgaacgc accgcgctgg ccgaggcgct ggagcggctg
     4741 ggcggcgccc ggcccggcgg ccggcgcacg gccgtccgcg cgggctacga gcaggtcgcc
     4801 gaccgcgcgg tccgccccac cgacctgggc ctgtacccgc cggaccggta cgccgacccg
     4861 gaattcccct atccggcgtt cacccccgac ctggagatca actgggtgtg ggggcactcc
     4921 ttcggccgcg gcggccccgt gctggtaccc gaggactacg cctactaccg cacccggcac
     4981 gggaacgaca cggcccgccc gctggcgtac gaggtctcca acggctgcgc cctcggcggc
     5041 tgtctggagg aggccgcgct gcacggcatc ctggagcttg ccgagcggga cgccttcctg
     5101 ctgacctggt acgcccggct gccggtgccg cgcgtggacc tggacaccgt ggccgacccg
     5161 gcggtccggc tgctggtgga gcgcatccgg caggacaccg ggcaccgggt cgccgcgttc
     5221 gccaccacgc ccgagcacgg catcgccacg tcctgggtga tggccgtcca ccccggggac
     5281 gcggtggagc ccgccgcgat ctgtgccgcg ggcgcccact tcgatcccga gaaggcgctg
     5341 ctcaacggcc tgttggagct gacagcgaac ctgggctgga accgcgccgc ctaccgcgag
     5401 gaactgcgcc ggatcgaggg catggtcgcc gacccgcacc aggtgcggga gatgcgcgac
     5461 cacgcgctgc tgtactgcca tccggacgcc tacgaccggt tcgccttcct gcccggcgag
     5521 gagacggccc ggccggtggc cgaggcgttc gcccgcgcca cctgccggcc gcgccacgcg
     5581 gacctgcgcg acgacctcgc cgaggtggtc gagcgcttca ccgcccgcgg cctggacgtg
     5641 atcgtggtcg accagaccac cgacgagcac cgcgccgccg gcttcgcctg cgcgaaggtg
     5701 atcatccccg gactgctgcc gatgaccttc gggcaccgga tgagccgtac ccacggcctg
     5761 ccccggctgt acgacctgcc ggcccggctc gggtaccggg acgggccgct ggagccggcc
     5821 gacgtcaacc cgcatccgca cccgttcccg tgacgccccg gccgtcctct tccccgtacg
     5881 gccggggccg ctccgccgca cgaccagaac cgacccgcac gcgaaggcag ggacaacgat
     5941 gagtggcttc cacgtcgcgg acacgttcgg cgtacgcatc ggtggtctgc cggtgtcggt
     6001 gctggacggt ttgcggtcgg acgaggtgtg gcggcggacc ggcgcgctgc tcgacggcgc
     6061 gcgggaactg gccgaccggg gcgcggaact ctccgacgcg ctctacgcgt tgatcgggca
     6121 ggagaccggc gtcaaggcgg cgctggtcgc gctgcgccgg gcggtgcaca accagcggac
     6181 gccgggcgag cgctgctgga acgacgaggt gcgcgccgcc ctgcccgccg aagtggtggc
     6241 ggacgtcgag cggtgggcgg agctgctggc ggcccaccgg gccggcaccg cagccctgga
     6301 cggcctgctc ccgcaggacg cggccgaccg ccgcgccgcc ctgcgcgagg cggccgccca
     6361 accggtgttc cggcacggcc tgatccaggg cagccctgtc ctccacgagc agttgcgcaa
     6421 gtggctggcg cgtcccgaag gcgccgtgcc ggaccgcaag ctggcgctcc ggctggccaa
     6481 gtacctcgcc cgggtggccg ccaagaccag cccgttcagc accttcaccg tcagcggcct
     6541 cggccggtgg gacggtccgc gcccggacgg ggacggaccg gcggtgcgcg acaccgtcga
     6601 gatcaacctg tggctcgtcc agcaacttgt ccggcggctg cgcgagcacc cggccctggc
     6661 gcccgccgtc cggctgcggg tcaacccgag cgcccaccag gccgacgggc actgggagtt
     6721 cctgggcccc ggcgaggagg agccgctacg caccctgccg gtgtccccgc cggtccaggc
     6781 ctgcgtggac ttcctcgccg acggtgcccg cccgcgccac gaggtggccg cccacctgac
     6841 cgagcggagc ggtgcctccc cggagcaggc cgagcgctat gtcgcgcggc tcaccgaact
     6901 cggtgtgctg gagaccgtgc cccccttctc cgagcaggcg atggacccgc tcgccgaact
     6961 gcgggcctgg gtggacaccg gcggcccgga gctgaccgag gtgtcggcgc ggctcaagga
     7021 ggtggccgcg ctgctggacg ggtacccgga gctgaccgac ccggacgccc gccggcggcg
     7081 caccgaggag atccaccggg cgctgggccg ggtcgccgac ctcgtcggcc cggagcaggt
     7141 catcgtgccg cccaagaact acatcatcga gaacgccctg ctcgtcgggc cgccgccggc
     7201 ccaggaccgg acggcgtggg aaccggtgct gcgcgacctg gacaccgtcc gccgctgcta
     7261 cgcggcgctc gacccggcgc tgcccggacg ggtggcgctg gcccggctgt tcaccacgcg
     7321 cttcgggagc ggcgcgagcg tgccgttcct cgcgttccac cgtgccgtcc aggaagagat
     7381 gcgtacggac ccgcagctgc ccggcctgct gtccgtctcg cagcacggct acgcgccgct
     7441 cgccgacagc ccgctgcccc ggatcaagga gctgctgcgg gtacgggccg ggctcaccgg
     7501 cgcggccctg aacgcacggc cggacgccga cggggtggtg cacgtcgacc ccgcgcggct
     7561 ggccgcgctg gccgacagct ggcccgcgtg gatgcggccg cccggctcgg tcgcgttcta
     7621 cggccagctg accggcgggt ccgaggaccc ggccgggttc gtggtcaacg ccgtcaacgc
     7681 ggggcacggg cggggccgcg accgcatccg gcggctgctc ggccaggccg gcgtcaccgt
     7741 ggcgggcgaa ccgtccggcc cgcccggcga cgtcatcctg gcggacacct gccgccatta
     7801 cggcagcaac gtgggcctgc gcgacctgac catgaccgac gaactcgact accccggctc
     7861 ggcgagcccg cgcccggcgg agcggcgcat tccgctgcgc gacctggagg tgcgccacga
     7921 cccggcgctg gacctcctcg tgctgtggtc gcgcacccgc gaccgcgagg tgcggccggt
     7981 gcaccccagc ctgatcgcgg agttctggct gccgccggcg atgcggctgc tggtccaggc
     8041 gttcggcgac acgccgacgc tgctgatccc cggccggcgg atgttcggcg acatctcccc
     8101 cgccacggca cagggcctgc tgcacgagcc caggatcacg atcggcacgg tcaccgtcag
     8161 ccgccgccag tgggtgttcc gcaccgacga ggcgccggta cgcgccaagg gcgaggcgga
     8221 ccgcccgtac ctgctgcggc tggccgcctg gctgcgcgaa cacggcatcc ccgagcgctg
     8281 cttcgtgcgc gcgctggacc cgtccatgct gaccgacggc aacgtctggc agctcaagtc
     8341 ccgcaagccg ctgtacatcg acttcgcgaa cctgctcctg accggactgt tcgagcgcat
     8401 gctcgccgag cggggcaacc tgctgttcct ccaggaagcc ctgcccgacc cgtccgccgc
     8461 gccgcactac ggcgacgccg gagcccgcgt caccgaacac ctcgtggagg tcggtcacca
     8521 tggccgctgc tgaccgcccc ctggaccccg ccaccgccgg tgcgtccgac tgggtgtgcg
     8581 cccacgtctt ccaggacacc ggccacgacg ccctgctcac cggctgtctg cggccgctgg
     8641 ccaaggagct gaccgccgac gggctgctgc accggtactt cttcctgcgg tactgggaag
     8701 gcggtccgca cgtacggatg cgcttcctgc ccaccggtac ggcggcctcc cgcgaggtac
     8761 ggcagcgggt ggtcacggcg gtcggcgcgt tcctgcggga ccacccggcc cctgacacgg
     8821 tggaccgcac gcggtacgcc gggctggccg gcgaactggc cgccctggag gacctctccg
     8881 agcacgaccg ggaactgcgg cccaacaaca cggtcgagtt cctgccgtac ctgcgcgagc
     8941 acgcggtgta cgggcacggt ccggccatgg ccgcgaccga gcagcacttc cacgagtcca
     9001 gcgaactggc cctggcgctg atcgcggccg gtacgaccgg ccagcagcgg gcggcgatcg
     9061 ccctcgacct gatgctggcc gtcctggccc tctgcgacga gctgcgcgcg cagtgggtcg
     9121 acagcggaca gccgctggtc cccttcgccg agggctccga acccgccgcc accgaggagc
     9181 actacctggc ccggcgggag cacctgcggg aacgcgcgct gcgcgtgtgg cagctcgccc
     9241 cggaccccac cggggcgacc ccgcaggcgc actggctgcg gtcgctgcgc acactgcggg
     9301 accggctcag cggctgggag gacgccggca cgttcacctc cgagtgggcc cgctccccgc
     9361 tgaaccaggg cccggacctg gccgcgctgc cgcacccggc caccacgctg atcctgctgc
     9421 gctgcgcgca cctggcgaac aaccggctcg ggctgaccct gagcgaggag aaccacctgc
     9481 gcttcctggc cggccggatc gtcaccgacc tgccgtcgct tccggtgcgc tgactcactg
     9541 tcccgcagcc cctcccggcc cgccgccccc gtacggcggg cccgcccgtc acgtcgacgg
     9601 gctccgtcac ctcgacgggc tgggccgcag ctccgcggac cgggggccgt cgtgccaccg
     9661 ggacggccgg accggggtgt cggcgcacgg ctgcccgggc ggcagggccc gggagcagga
     9721 gcacggtgcg ggcccggtgt ggcgggggcg gccgtcggcc ggctgggccg ccgggcgtgc
     9781 ctggtcctcc ggcggctcgg cggacggctc cgccggaatc gccgcggcca gcccctgccg
     9841 ccccgctatg cccagcttgc ggtacacacc ggacaggtgg aactccaccg tacgcatggt
     9901 caggaacagc atctgggaga tctgccggtt ggtgtgtccg ctccgggcca gcccggcgat
     9961 cttcaactcc tgcggggtca gcagcggatg agtacggacc ggcttgtcgt gggccggtac
    10021 gggctccggc gccgcacgca gccgccgggc cagcggcgtg gcaccgcact caccggccag
    10081 ggccagcgcc gccgcgcact cgcggtccgc ctcggccgtc cgcccggcgg cgcgcagcgc
    10141 ggcgcccagg tcggccctgg ccacggcgag ttccaggcgg gcgggtgacg cttcgaggtg
    10201 gcgcaccgcc tcggtcagca acatcagcct gcgctccccc gaccgggtca tcgccagggc
    10261 gtgccgggcg acgccgatcg agcggggtgt cccccagcgg cgggcggcct ccagttcctc
    10321 gcgcgccagg gtctcggccc ggtcggtggc gccgtcggcg gccagggcga gcgccgcgcg
    10381 ggagcgccac ggcagcaccg ccgggctgac cgcgccccgg cgcaccaact gccgtccggc
    10441 ggccagcaga tccacggccg cgcggcccgg ctcgccgcgc agccgccaca gggccgcccg
    10501 ggcggcgagc aggtacgggc cctcgtagcg tgcgtgcagg tcgtcgtccg ggccgtggcc
    10561 ggcgacgccg tgcgcggcca gcagcgcggc ggcctcgtcc tggtcgccca gctgcgccag
    10621 cgcctccacc agaccggcca ccgcgaagcc ggcgaccatc ggcagcgcgg cgctgccggg
    10681 cccgatctcc tcgaccaccg gccgcaggat cgccaccgca ccgggcaggt ccccggcgac
    10741 ccggcagatc cgggcccgga agatgtccgc cgccagcccg gcccgccggc ccgacatggc
    10801 gcggctgagc ctgccggtgc gccagcagtg ggcccgcgcc tcgacgagtt cgtcggcgcg
    10861 gatcagggtc gcgcagctct gccagaggcg cccgaggtcg ccgccttccg cgccggtcag
    10921 cgcctcgcgc gcgggggcga gggccgcgtc ccggtccgtt ccccggtcgg cggcgatcgc
    10981 ggcccgcagg ttggccagtt cccggctccg tccgccgccg gacggctcgg ccgcgtccgc
    11041 gtccgccagg cggcgggcct cggcggaggg gccggccgcg tcgagcaggg agacgctcgc
    11101 cttccggagg gtgagcctgc gcaccgccgc cgggtggccg gcgccgatcc gttcggccgt
    11161 ctcgtccagt acggaggcgg cgtccgccgg tctggcccgg tcgatgagca tgccggccac
    11221 gtccagggcc agttcggcgc agacctcggg gtcctcggac tcgcgcagca gggtgtccag
    11281 ctcgctcagc gcgaggtccg ggtcgacgaa ggtctcggcc tcggccagtt cgcgccggac
    11341 ggccgagcgg acgtccgcgg gcagccggtc ccgcagcacg caccgcagat agcggctcgc
    11401 ctcctcggcc cgcccctcgt gcaggagttt gcgtgccgcg gcacgcagcg gctcgaccgc
    11461 ccagtccagc ccggtcgggc ccgccgcggc gagctgctgg gcgacccgtt ccgcgcccca
    11521 ctgctcgtcc cgcaggaacc gggccacctt caggtgcgcg gcgatccggg agccgacggg
    11581 catcccggcg agcaccgcgg tgcccagcag cgggtgccgg aacgccggcg gcacccggtt
    11641 ggcgagcacc tgccgtgcca cgagggtgtc gatcgcgtgc agggcgtcgg ggaccggcag
    11701 ctcggccagc gcggcggcgt gggcgacctc cgcgctgtcg cccagaaccg cgacagccct
    11761 ggccatggcc agggctggtc ggggcaggtc gtcgagccac cgccgggtac atcgcagcgc
    11821 ttcgggtccg gccgcgccgc gcagttcgcg cgcggtgacc gccgggccgg ggcggcccgc
    11881 caggtgggcg agcagcaggc cggggttgcc gccggtcagt tcgtggcaca cggcggccgc
    11941 cgcggcgtcg agggcgaggc cgcagtgccg cgcggcgaac tcggcgatcc cggcctcggg
    12001 caggccggtc agctccaggt gctcggcgcg ggcgaggagt tcggccgcgc cggccgtccg
    12061 gccgctgccc gggtcgtcgc cggccgccgc gacgatcagc agcggtacgg gccggtcgag
    12121 cgaggccagg gccaccagcc aggtcgccga cgacgcgtcc acgtccatga ggtcgtccac
    12181 gcacagcgcg aggggcgtgc gtgccatgac gtcccggccg cgggcgacgt gaccgtccag
    12241 ggtgtccgtc ccgtcgtccg ggcggccgag ggcctgacgg gccgcggcga acagggagtc
    12301 ccgtacggcc ggtcccccgg cggcggtccc cacccggaag cccgcgtccg ccgcgcgggc
    12361 cgccaccgct tccagcaggg cgctcttgcc gacgccccgc tccccccgca ccgcgacgat
    12421 caccggtacg ccgccgccgg cccgcccgat cagcccgtcc agccgggcca gctccgcttc
    12481 acgaccggtt aacggcat
//
