Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001153.1:
Cluster 1NRP140826

BGC0001153, polymyxin biosynthetic gene cluster from Paenibacillus polymyxa. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: polymyxin B
PubChem ID: 9833652
SMILES string: Copy to clipboard
Molecular formula: C56H98N16O13
Average molecular mass: 1203.499 Da
Molecular activity: Antibacterial
Molecular target: bacterial cell envelope

Class-specific details

Biosynthetic class(es):
NRP
Nonribosomal peptide subclass:
Other lipopeptide (cyclic)
isooctanoic acid
Thioesterase type:
Type I
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
pmxE (AEZ51520.1)
Module 0
A specificity: 2,4-Diaminobutyric acid
Evidence for specificity: Sequence-based prediction
C domain subtype: Starter
Module 1
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 2
A specificity: L-2,4-Diaminobutyric acid
Evidence for specificity: Sequence-based prediction
AA is epimerized
C domain subtype: LCL
Module 3
A specificity: L-2,4-Diaminobutyric acid
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 4
A specificity: L-2,4-Diaminobutyric acid
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 5
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
pmxA (AEZ51516.1)
Module 10
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
Module 6
A specificity: Phenylalanine
Evidence for specificity: Sequence-based prediction
AA is epimerized
C domain subtype: N/A
Module 7
A specificity: Leucine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 8
A specificity: L-2,4-Diaminobutyric acid
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 9
A specificity: L-2,4-Diaminobutyric acid
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
pmxB (AEZ51517.1)
Module 11
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: N/A

Gene cluster description

polymyxin (BGC0001153). Gene Cluster 1. Biosynthetic class = NRP. GenBank JN660148, positions 1-40826. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?unknown
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Susan Jensen (University of Alberta)

Literature references

1. Shaheen M et al. (2011) Paenibacillus polymyxa PKB1 produces variants of polymyxin B-type antibiotics. Chem Biol 18(12):1640-8. doi: 10.1016/j.chembiol.2011.09.017.