Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001155.1:
Cluster 1RiPP126287

BGC0001155, GE2270 biosynthetic gene cluster from Planobispora rosea. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: GE2270
PubChem ID: 16130335
SMILES string: Copy to clipboard
Molecular formula: C56H55N15O10S6
Average molecular mass: 1290.511 Da
Molecular activity: Antibacterial
Molecular target: EF-Tu

Class-specific details

Biosynthetic class(es):
RiPP
RiPP subclass:
Thiopeptide (cyclic)
Peptides in this cluster:
AGY49587.1
RiPP core peptide sequence(s): SCNCVCGFCCSCSPSA
Leader peptide length: 39
Follower peptide length: -1
Peptidase(s) involved in precursor cleavage:
Crosslinks in final product:
1=11 AA (Other)

Gene cluster description

GE2270 (BGC0001155). Gene Cluster 1. Biosynthetic class = RiPP. GenBank KF366381, positions 4201-25722. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Detailed annotation

pbtA leader / core peptide, putative Class I
MSEMELNLNDLPMDVFEMADSGMEVE - DhaLDhbAGHGMPEVGADhaCNCVCGFCCDhaCDhaPDhaA

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Margherita Sosio (Naicons Srl)

Literature references

1. Tocchetti A et al. (2013) Capturing linear intermediates and C-terminal variants during maturation of the thiopeptide GE2270. Chem Biol 20(8):1067-77. doi: 10.1016/j.chembiol.2013.07.005. Epub
2. Flinspach K et al. (2014 TI) Heterologous expression of the thiopeptide antibiotic GE2270 from Planobispora rosea ATCC 53733 in Streptomyces coelicolor requires deletion of ribosomal genes from the expression construct. PLoS One 9(3):e90499. doi: 10.1371/journal.pone.0090499. eCollection
3. Morris RP et al. (2009) Ribosomally synthesized thiopeptide antibiotics targeting elongation factor Tu. J Am Chem Soc 131(16):5946-55. doi: 10.1021/ja900488a.