BGC0001159 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001159 (Streptomyces sp. CNQ-418)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	43,083	High	marinopyrrole A/marinopyrrole E/marinopyrrole D/marinopyrrole C/marinopyrrole B	NRPS:Type I+PKS:Type I

BGC0001159 - Region 1 - T1PKS

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Location: 1 - 43,083 nt. (total: 43,083 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 43,084
Label		BGC0001159

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

AFP87517.1: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

mpy1: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

mpy3: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: PPTase halogenase

mpy10: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

mpy11: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

mpy16: flavin-dependent pyrrole halogenase

halogenases: flavin-dependent pyrrole halogenase
smcogs: SMCOG1119: halogenase

mpy5: halogenase

smcogs: SMCOG1119: halogenase

mpy8: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: esterase

mpy9: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

mpy2: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

mpy6: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

mpy7: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001159.5	1.00	NRPS, PKS	marinopyrrole A, marinopyrrole E, marinopyrrole D, marinopyrrole C, marinopyrrole B	Streptomyces sp. CNQ-418
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001159.5	1.00	NRPS, PKS	marinopyrrole A, marinopyrrole E, marinopyrrole D, marinopyrrole C, marinopyrrole B	Streptomyces sp. CNQ-418
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 43083:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (ohmal)
Non-colinear ordering:: mpy7 + mpy6

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mpy2: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (8..445): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLFYVAWVCK
Nearest Stachelhaus code(s):: Pro DLFYVAWVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mpy6: mal

PKS_AT (574..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.3
inactive: 91.8
Methoxymalonyl-CoA: 78.0
Methylmalonyl-CoA: 70.6
Isobutyryl-CoA: 52.4
Propionyl-CoA: 48.9
Ethylmalonyl-CoA: 32.3
trans-1,2-CPDA: 17.2
Acetyl-CoA: 16.9
Benzoyl-CoA: 14.0
fatty_acid: 13.8
CHC-CoA: 13.1
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.4

mpy7: pk

PKS_AT (554..850): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 58.3%; Propionyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 94.6
Malonyl-CoA: 91.3
Methoxymalonyl-CoA: 78.4
Propionyl-CoA: 54.2
inactive: 43.1
Ethylmalonyl-CoA: 39.4
trans-1,2-CPDA: 29.4
Isobutyryl-CoA: 24.2
2-Methylbutyryl-CoA: 24.0
Benzoyl-CoA: 21.9
Acetyl-CoA: 18.7
CHC-CoA: 16.1
fatty_acid: 12.6
3-Methylbutyryl-CoA: -0.1

Link to PARAS substrate predictor