LOCUS       BGC0001167              9324 bp    DNA     linear   BCT 06-SEP-2012
DEFINITION  Microcystis aeruginosa PCC 7005 piricyclamide precursor (pirE2)
            gene, partial cds; piricyclamide precursor (pirE3), putative prenyl
            transferase (pirF), hypothetical proteins, N-terminal cyanobactin
            protease (pirA), hypothetical protein (pirB), hypothetical protein
            (pirC), piricyclamide precursor (pirE4), and hypothetical protein
            genes, complete cds; and C-terminal cyanoabactin protease (pirG)
            gene, partial cds.
ACCESSION   BGC0001167
VERSION     BGC0001167
KEYWORDS    .
SOURCE      Microcystis aeruginosa PCC 7005
  ORGANISM  Microcystis aeruginosa PCC 7005
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Chroococcales; Microcystaceae; Microcystis.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 15:02:03
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9324
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            <1..138
                     /gene="pirE2"
     CDS             <1..138
                     /codon_start=1
                     /gene="pirE2"
                     /product="piricyclamide precursor"
                     /protein_id="AFK79987.1"
                     /transl_table=11
                     /translation="MNSAPVQRENAATVSRDGNAIAPQWILLADGTRPKNAPFAGDDAE
                     "
     source          1..9324
                     /db_xref="taxon:267870"
                     /isolation_source="freshwater"
                     /mol_type="genomic DNA"
                     /organism="Microcystis aeruginosa PCC 7005"
                     /strain="PCC 7005"
     subregion       1..9324
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9324
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            387..542
                     /gene="pirE3"
     CDS             387..542
                     /codon_start=1
                     /gene="pirE3"
                     /product="piricyclamide precursor"
                     /protein_id="AFK79988.1"
                     /transl_table=11
                     /translation="MKTKKLTPRNSAPVQRENAATVSRDGNAIAPNNEFMQTGSYSGPF
                     AGDDAE"
     gene            792..1682
                     /gene="pirF"
     CDS             792..1682
                     /codon_start=1
                     /gene="pirF"
                     /product="putative prenyl transferase"
                     /protein_id="AFK79989.1"
                     /transl_table=11
                     /translation="MIVAEIQKNSLKEQRIKFIRNHQQAFDVEPIYPLRLFEDFVMSVE
                     GDCSIEASCKIELDKLIASRFMLFFKDQEWEKYLTQSLAFFRQVENRVGVQLDYSLLQK
                     FLGHNFDFSKLEVLSAGLDLRTNLADSSLKIHIRIKDYPEKINQALSLTIDGDDLTAVR
                     DFLSVVGFDFYFDGRSAIEIYPEVKEEDFFKPKTQEKVWQHLPKFVLEPLQVTNLFGFG
                     FSKTNHNPVVYYRLKGRQDLTNYFKINDTAQRVHSFYQHQDILPNMWVGTTQKELEKTR
                     IENIRLYYYKSFKME"
     CDS             1694..2152
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="AFK79990.1"
                     /transl_table=11
                     /translation="MIEVNELKQLYDVDDDQWLEQTINLLKNHQFQQLDLDNLIEELED
                     LGREKKNAVASLLEQIIRHLLLLQYWTTEAEYNTVHRQEEIYNFRTQLRRTITTNLRKY
                     LKDELTSIYQDALGFVKIKTTNLVTFPTECPYSQEQLLDRSWLPNQTY"
     CDS             2208..2786
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="AFK79991.1"
                     /transl_table=11
                     /translation="MITTEPIILNLKTTNLSDEQFYQLCCANDDWRLEQTAEGELIIMP
                     PIEAISGNRESELNADLVIWNRQTQLGKVFSSSTIFTLPNGAKRSPDVAWIANERWHAL
                     PIEEQEKFARICPDFVIELRSRTDPLKYLQEKMQEYRENGVKLGWLIDPANQRLEIYRP
                     NQSVEIIELPATLSGEEILPGFTLKLPMY"
     CDS             2805..3488
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="AFK79992.1"
                     /transl_table=11
                     /translation="MIATVAKSPQAIPLLENGDNLTRCEFERRYNAMPNLKKAELIEGI
                     VHLMASPLRITNHGNPHARIITWLGNYWSSTPGIELGDNCTVRLDADNEPQPDALLRIK
                     NGGQSTISADGYVEGAPELIVEIAASTVSLDLHQKLKVYRCHQVQEYLVWRVLDEEFDW
                     FVLNDEKYVTLEPNAEGILCSQIFPGLWLGRDALLAGNLARVLEVVRLGIATIQHKSFV
                     KKISE"
     gene            3494..5431
                     /gene="pirA"
     CDS             3494..5431
                     /codon_start=1
                     /gene="pirA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 172.3; E-value: 3.7e-52)"
                     /gene_kind="biosynthetic"
                     /product="N-terminal cyanobactin protease"
                     /protein_id="AFK79993.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 8.7e-38, bitscore:
                     119.8, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.7e-220,
                     bitscore: 722.1, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPGLQALHKLTQGSPEITVAILDGVVDTQHPCFQGAKLTRLPTL
                     VLHGATSGQMSTHGTHIASLIFGQPNTEIEGIAPQCHGLLVPVFSDDRRRLSQLDLARA
                     IEQAAENGAHIISISGGALTDMGEAEDWLERAIEMCSQRNILVVAAAGNDGCECLHVPA
                     ALPAVLAVGAVDARGHPLDFSNWGETYQNQGILAPGENILGAKPGGGTIQMSGTSFAAP
                     IVAGVAALLFSLQVQRGETPNPHAVREALLKSALPCQYADSENEPKCLAGLLNISGAIK
                     HLTGETMSETIETQATVEASGCGCGGTPETTEVQRQNLELSAATSAPAIPVVAAQIPNP
                     VTTSQGVSMPETTTKSVTPSQAAEPAGGIVYAIGTLGYDFGTEARRDTFKQLMPTAVIG
                     GIEVPSNPYDARQMVDYLADNLSEAKSLIWTLNLELTPIYAIEPLGSFSREVYSALQEL
                     LSGQVQAEDSPEYIQRVSIPGRITGRTVRLFSGQVVPVIEPDSPRGIYGWHVNTLVRAA
                     IEAVGAEQTEAQESQMRRTLSSFLNRIYYDLRNLGQTSQDRALNFAATNAFQAAQTFSE
                     AVGAGMELDSINVSKSPFCRLDSDCWDVQLKFFDPENSRRARKVFRFTIDVSDLIPVTL
                     GEVRSWSSPY"
     gene            5560..5769
                     /gene="pirB"
     CDS             5560..5769
                     /codon_start=1
                     /gene="pirB"
                     /product="hypothetical protein"
                     /protein_id="AFK79994.1"
                     /transl_table=11
                     /translation="MRLPKQSAPVKRPHFIHPHECVDIIHGDGEDLVSILVKLVHGANY
                     NDPAQFQYPHYGYLKMSSFGGFRY"
     gene            5816..6037
                     /gene="pirC"
     CDS             5816..6037
                     /codon_start=1
                     /gene="pirC"
                     /product="hypothetical protein"
                     /protein_id="AFK79995.1"
                     /transl_table=11
                     /translation="MANSKSSKQSETNPNVEQPETKKSNIQPQETTQPKAKYIPATGLE
                     DYGRWKLMFQDRKATPNDPPFRRGRIWC"
     gene            6159..6314
                     /gene="pirE4"
     CDS             6159..6314
                     /codon_start=1
                     /gene="pirE4"
                     /product="piricyclamide precursor"
                     /protein_id="AFK79996.1"
                     /transl_table=11
                     /translation="MKTPKLTPRITAPVQRDNIATVPSDGNVITPATFCDLATKQCYPF
                     AGDDAV"
     CDS             complement(6396..7187)
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="AFK79997.1"
                     /transl_table=11
                     /translation="MSSIPQTVQSSTPIQEPSATVYCDSPLPDHTQLPDKDGSFVKNYQ
                     QPPPADLLTDTITPLLEQLHPDGNYCLGRDCGIYWRLPEHPESLEKGAVAPDWFYVPHV
                     PTSIDGQVRRSFVMWKETIEPVIALEFVSGNGEEERDKTPVKGKFWTYERAIQIPFYGI
                     YEVNKAQIEIYQLVNGRYQKMTPNQRGHYPIVPLKVELGIWQGEYQNQFFPWLRWWDGE
                     GNLLLTSQESTEQERQRAERERQRAERLAEQLRALRIEPED"
     gene            7255..>9324
                     /gene="pirG"
     CDS             7255..>9324
                     /codon_start=1
                     /gene="pirG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 105.6; E-value: 6.1e-32)"
                     /gene_kind="biosynthetic"
                     /note="macrocyclaze"
                     /product="C-terminal cyanoabactin protease"
                     /protein_id="AFK79998.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.6e-26, bitscore:
                     82.9, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.8e-212,
                     bitscore: 695.6, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDLNAIPGMTALRSHTKGDPRIKIAVLDGPIDLDRACFQGANIT
                     RINPYWAEEFPIDPQHLQSFLEIEASGKSDEEKEEMFKEAIPDENIRHSLHLGFHANHI
                     ISTICGQPDSPVEGIAPNATVINIPIAYGNDDFINPLNLSRAISTAIEQGVNIIHCAAC
                     HPTQSGLAHEFIDKAIRQAQDNNILVIAPGGNDKGECWCIPAVLENVLTVGAMKDTGEP
                     FKFSNFGGKYATQGILAPGENILGSQPGTDDPIRKKGTSCAAPIVTGTAALLMSLQLEQ
                     GLSPDAEAVRAALINSAIPCDSNEVEELERCLLGKINIAGAYELLTGEKLQTVEADNNL
                     SNNIIKSAVIPMTVTTSVIPSNTTEMATQGITITPPTAATIELAIAPSNASENVSSNGL
                     IQNNQTNNRLFFNSVVPSQRSNLVYVLGNLGYDFGTEARRDTFKQLMPSIMIDNLELPS
                     NPYDARQMVDYLESNLSETKSLIWTVNLELTPIYAIKPVGAFAADIYQVMQYMLAGQIL
                     PEADEDYVERVSIPGMLTEETVKLFSGQIIPVLKVNSPRGMYGWKVNTLIESAIETVSA
                     EHEIADEARMRRSLTSFLNRIYYDLRNLGQIARDRALNFAATNAFQAVQTFSQAVAMGM
                     ELHSIEVEKSPFCRYGSECWDVKLKFFDPENGLRAKRVFRFTIDVSDRTPVSVGE"
ORIGIN
        1 cgtaactctg ctcccgtcca acgggaaaat gccgctaccg tctctcgtga tggcaatgcg
       61 atcgcccctc aatggattct gttggccgac ggtacgcggc caaaaaatgc tccttttgcg
      121 ggagacgacg cagagtaatc ctcccgattc gagcatcgga cttcaaatca tgtcaaaagt
      181 ctttttctgc ttgccagggt gcgcggtttg ccccaacgaa ttgacgtaca acccttgtta
      241 attcagagct ttctgttagg aataagggag aaagtgtgat tcgcaaactt gactctttct
      301 caacaaaaat tgacctagaa actgttgact tggattggta gtgttgcctt actgtgaaaa
      361 acaaacctat tggaggattt taccccatga aaaccaagaa actgacaccc cgtaactctg
      421 ctcccgtcca acgggaaaat gccgctaccg tctctcgtga tggcaatgcg atcgccccga
      481 ataatgagtt tatgcagaca gggagttaca gcggtccttt tgcgggagac gacgcggagt
      541 aatccccctc attcgagcat cggacttcaa atcatgtcaa aagtcttttt ctgcttgcca
      601 gggtgcgcgg tttgccccaa cgaattgacg tacaatcctt gttaattcag agctttctgt
      661 tacctggcac gcgggagaag cttttgagcg aagtgattaa aaaagtcggg gattttctta
      721 aactctgaga atcaaaatca aagatttaca taaatccccg acttttcatc tttaatctaa
      781 cacttgaaat tatgatagtt gctgagattc aaaaaaactc cctcaaagag caaagaataa
      841 aatttatccg taatcatcaa caagcttttg atgttgagcc gatttatcct ctgcggctgt
      901 ttgaagactt tgtaatgagt gttgagggtg actgtagtat tgaagcttct tgtaaaatag
      961 aactggacaa attaattgct tcacggttta tgctattctt caaagatcaa gagtgggaaa
     1021 aatatctaac tcaatcttta gctttttttc ggcaagttga aaaccgagtt ggtgttcaat
     1081 tagattattc tttattacaa aaatttttag gacataactt tgactttagc aaactagaag
     1141 ttctcagtgc tggacttgat ttaagaacta atttagctga ttctagttta aaaatacaca
     1201 ttagaattaa agattatcct gaaaaaatca accaagcttt atcattaact attgatggcg
     1261 atgacttgac agctgtacgg gattttctgt ccgtagtcgg ctttgatttt tattttgatg
     1321 gtcgtagtgc tattgaaatt tatcctgaag ttaaagaaga agattttttc aagccaaaaa
     1381 ctcaagaaaa ggtatggcaa catttgccaa aatttgtttt agaacctctt caggttacaa
     1441 atttgtttgg ttttggtttt tccaaaacta atcataatcc agtggtttac taccgtctca
     1501 aaggtcgtca agatttaacc aattatttta aaatcaatga tacagcccaa agagttcaca
     1561 gcttttatca acatcaagat attttaccta atatgtgggt aggtactact caaaaagaat
     1621 tagaaaaaac gagaattgag aatattcggc tttattatta caaatccttc aagatggagt
     1681 aaattttatg ttgatgatag aagtaaatga attaaaacaa ctctatgacg ttgatgatga
     1741 tcaatggctg gaacaaacta ttaacttact taaaaatcat caattccaac agctagattt
     1801 agataattta attgaggagt tagaggattt gggtagagag aagaaaaatg ctgtcgctag
     1861 tctgttagag caaattattc gtcatttgct gttactgcaa tattggacaa ctgaagccga
     1921 atataatact gttcaccggc aagaagaaat ctataacttt cgtactcaac tgagacgaac
     1981 aatcacgact aatctccgca agtatttaaa agatgaatta acctctatct atcaagatgc
     2041 gctaggtttt gttaaaatta aaacaactaa tttagtaact tttcctactg aatgccccta
     2101 ctcccaagaa cagttacttg atcgttcttg gttgcccaat caaacctatt aactgacaat
     2161 caatctaaaa caagaactaa aaatattgac tttaaaaata aagtataatg attaccaccg
     2221 aacctattat cttaaacctg aaaaccacca atttaagtga cgaacagttt tatcaactgt
     2281 gctgtgctaa tgacgattgg cgactcgaac aaactgccga aggagaatta atcattatgc
     2341 ctcccatcga agccattagt ggcaatcgag aatcagaatt aaatgctgat ttagtgattt
     2401 ggaatcggca aactcaactc ggtaaagttt ttagttcttc tactatcttt accttgccca
     2461 atggcgcgaa acgttctcct gatgttgctt ggattgccaa tgaacgctgg cacgctttac
     2521 ccatagaaga acaagaaaaa tttgccagaa tttgcccaga ttttgtgata gaattgcgat
     2581 cgcgtaccga ccccttaaaa tatctacaag aaaaaatgca agaatatcga gaaaatggtg
     2641 taaaattagg gtggctaatt gatcctgcca accaaaggtt agaaatctat cgccccaatc
     2701 aatctgtaga aattattgaa ttaccagcaa cattatcagg agaagaaatt ttacctggat
     2761 tcaccttaaa attaccaatg tattgacaag taggaaatac taatatgata gccactgtag
     2821 caaaatctcc tcaagcaatt ccactattag aaaatggcga caacctcact cgttgtgaat
     2881 ttgagcgtag atataatgct atgccaaatc tgaaaaaagc cgaactcatt gaaggaattg
     2941 ttcatctgat ggcttctccc ttaagaatta ctaatcatgg caaccctcac gctcgtatta
     3001 tcacttggtt aggtaactat tggtcaagta cgccaggaat tgaattaggc gacaattgta
     3061 ccgtgcgcct tgatgctgat aatgaacctc aacccgatgc actgctgaga attaaaaatg
     3121 gtggacaatc aactattagt gcggatggct atgtcgaagg tgcgccagaa ttaatcgtgg
     3181 aaattgctgc tagtaccgtc tcccttgact tgcatcaaaa gcttaaggtt tatcgctgcc
     3241 atcaagtgca agaatattta gtctggcgag tattggatga agaatttgat tggtttgtct
     3301 taaatgacga aaaatatgtt acacttgaac ctaatgcaga aggaatatta tgttctcaaa
     3361 tatttcctgg attatggtta ggtagagatg ctttattagc gggtaattta gccagggttt
     3421 tagaagttgt acgattggga atagctacaa tccaacataa atcttttgtc aaaaaaatat
     3481 cagaataaga attatgattc caggattgca agcattacac aaactaaccc agggttcccc
     3541 agagattact gttgccattc ttgatggtgt cgtagatact caacatccct gttttcaggg
     3601 agcaaaatta actcgactgc ccaccttagt attacatggt gcaaccagtg ggcagatgtc
     3661 cacccatggg actcatattg ctagtttaat ttttggacag cctaatacag aaatcgaagg
     3721 aattgcccct cagtgtcacg gtttactcgt gccagttttt tccgatgacc gacggcgact
     3781 atctcaacta gatttagccc gtgcgattga acaagccgcc gaaaatggag cgcacattat
     3841 tagtattagt gggggggcgc tcaccgatat gggagaagca gaagactggc tagagcgtgc
     3901 cattgaaatg tgtagccaga gaaatattct tgtcgttgcg gctgccggaa atgatggttg
     3961 cgagtgcctc catgttccag ccgcccttcc tgctgtccta gcagtgggtg ctgttgatgc
     4021 tagagggcat cccctcgact ttagtaattg gggggaaacc taccaaaatc aaggcatcct
     4081 tgcccctgga gaaaacatct taggggcaaa accgggcggc gggacaatac aaatgagtgg
     4141 cactagcttc gctgcgccca tcgtagcagg agtagcggct ttattattca gtttgcaagt
     4201 gcagcgggga gaaacaccta atccccacgc agtcagggaa gcactactaa aaagcgccct
     4261 tccctgtcaa tatgcagaca gtgaaaacga accaaaatgt ttagccggcc tgttgaacat
     4321 ttcgggggca attaaacacc taaccggaga aaccatgtcc gaaaccatag aaacccaagc
     4381 aacagtagaa gcttccggct gcggctgcgg tggtacacct gaaaccaccg aggttcaacg
     4441 ccaaaatctc gaactgagtg ccgcaaccag cgcaccggca atccccgttg ttgccgccca
     4501 aattcccaac cctgtaacaa cctctcaagg agtatccatg cctgaaacta ccactaaatc
     4561 tgtcacccct agtcaagctg ctgaacccgc tggtggaatc gtctatgcca ttggcacttt
     4621 gggctatgat ttcggtacag aagcccgtcg ggacacattt aaacaactga tgcccaccgc
     4681 cgtaattgga ggaattgaag ttccctctaa tccctacgat gcccgccaga tggtggatta
     4741 tttagccgat aacctctcag aagccaaatc cttaatctgg accctgaacc tggaacttac
     4801 ccccatttac gccattgaac ccctcggctc tttttcccgc gaagtttatt cggctttaca
     4861 ggaactgctt tcgggacaag tgcaagcgga agacagcccc gaatatatcc agcgtgtcag
     4921 tattccggga cggattactg ggcgcacagt acgcctgttc tcaggacaag ttgttcccgt
     4981 cattgaaccg gatagccccc ggggaattta tggttggcac gttaatactt tagtccgtgc
     5041 ggcaatagaa gctgttggtg cagaacaaac agaagcccaa gaaagccaaa tgcgccgcac
     5101 tttgagtagt ttcctcaacc gcatctacta cgatttacgc aatttagggc aaacttccca
     5161 agaccgcgcc ctcaattttg ccgcgaccaa tgctttccaa gcagcgcaaa ctttttctga
     5221 ggctgtgggt gctgggatgg agttagatag tattaacgtt tctaagagtc ctttctgtcg
     5281 gctggatagc gattgttggg atgtacagtt aaaattcttc gacccagaaa atagccgccg
     5341 cgctcgtaaa gtattccgtt ttactataga tgtgagtgat ttaattcctg ttactttggg
     5401 cgaggttcgt tcctggtctt ctccttatta gtcctctccc ctgcgctgac ttctgacttc
     5461 aagtacgggc gaagcattcg gataggaaat ctaaggtttc agcgataggt tatgcccgaa
     5521 tgcttcgccc ctacccccat tatttacccg aaaacaacca tgagacttcc caaacagtct
     5581 gctcccgtca aacgtcccca ttttattcat ccccatgaat gcgtcgatat tattcacggt
     5641 gatggcgaag acttggtatc tatcttagtt aagttagttc atggagctaa ctacaatgat
     5701 ccggctcagt ttcaataccc tcactatggc tatctgaaaa tgtcctcttt tggcggtttt
     5761 cgttattagt atcgacttct aaaacctagt aaaaaacact ggaggaaatc aaacaatggc
     5821 taactctaaa agttctaagc aatcagaaac caatccgaat gttgagcaac cagaaacaaa
     5881 aaaatccaac attcaaccac aggaaacaac tcaacctaaa gctaaatata ttcccgcaac
     5941 tgggttagaa gattacggac gctggaaatt gatgttccaa gaccgcaagg cgactcccaa
     6001 tgatccgccg tttcgtcggg gtcgaatttg gtgttaaact ttgtccattt ttcctgcaaa
     6061 caaattgttt aaagttgaca atagttaaca gaaaactctt gacaaggatc gtgtaaatct
     6121 tttagtgtta tcgacaacca cctttggagt aacttatcat gaaaacccca aaactgaccc
     6181 ctcgcattac cgccccggtt caacgagaca acatcgctac tgttcctagt gatggtaatg
     6241 tgattacccc tgctaccttc tgtgatttag ccaccaagca atgttatccc tttgcgggtg
     6301 acgacgcagt gtaatttgtt cttcctggca ttagtcatta ggtaggcagt atttttttac
     6361 atgaattgtt cgtgcttgcc gacctcccaa aattactaat cttcaggttc tatccgtaat
     6421 gcccttaact gttccgctag gcgctcggct cgttgtcgtt cccgctcggc tcgttgtcgt
     6481 tcctgttcag tagattcctg ggaagttaac aacaagttac cttccccatc ccaccagcga
     6541 agccagggaa agaattgatt ttggtattct ccttgccaaa ttcctaactc tacctttaaa
     6601 ggaacaatag gataatgacc gcgctgatta ggagtcattt tttggtaacg accattaact
     6661 aactgataga tctcgatctg ggccttgtta acttcataaa ttccataaaa aggaatttga
     6721 atcgctcgct cataggtcca aaatttaccc ttaacaggcg ttttatcccg ttcttcttca
     6781 ccattgccag agacaaattc taaggcaata accggttcaa tggtttcttt ccacatcaca
     6841 aaggaacggc gaacttgacc atcaatagac gtggggacat ggggaacata aaaccaatcg
     6901 ggagcaacag cacccttttc tagagattct ggatgctctg gcaatcgcca ataaatccca
     6961 caatctcgac ctaggcagta attaccatca ggatgtaatt gttctaaaag gggtgtaata
     7021 gtatccgtta aaaggtcggc ggggggaggc tgttgatagt ttttcacaaa acttccatct
     7081 ttatcgggga gttgggtgtg gtcgggtaag ggggaatcgc aatacaccgt tgcagaaggt
     7141 tcctgaattg gggttgagga ttgtaccgtt tgggggattg aactcatctt taattcccct
     7201 atttttgtta tctagctttt attctataac tgttcttgaa aatctgtaca aactatgcct
     7261 gatctcaatg ctatccccgg aatgaccgct cttcgctccc ataccaaagg cgatccacgc
     7321 atcaaaatcg ccgttcttga tggaccgatt gacttagatc gcgcctgttt tcaaggagca
     7381 aatattaccc gtattaatcc ttattgggcc gaagaatttc ccatcgatcc ccaacactta
     7441 caatccttct tagaaataga agctagtggc aaaagtgatg aagaaaaaga ggaaatgttt
     7501 aaggaggcga ttccagatga aaatatccgc cacagtttgc atttgggctt ccatgctaac
     7561 catattatca gcaccatttg tggacagcca gattcaccgg tagaagggat cgctcctaac
     7621 gcaacggtga ttaatattcc cattgcttat gggaatgacg attttatcaa tcctctcaat
     7681 ctttctcgtg cgattagcac cgctattgaa cagggagtta atattattca ttgtgccgct
     7741 tgtcacccaa ctcaaagcgg attagctcac gaatttattg ataaagcgat tcgtcaagca
     7801 caggataata atatactcgt tattgctcct ggtggtaatg ataaaggaga gtgttggtgc
     7861 attcctgcgg tattagaaaa tgtcctgaca gtaggagcga tgaaagatac aggagagcca
     7921 tttaaattca gtaattttgg tggtaaatat gcaactcaag ggattcttgc tcccggagag
     7981 aatattctag gatctcaacc cggaactgat gaccctatcc gcaaaaaagg gactagctgc
     8041 gctgctccaa ttgtgacagg aacggccgct ttattaatga gtttacaatt agaacagggt
     8101 ttatcgcctg atgcggaagc agtaagagcc gctttgatta atagtgccat cccttgcgat
     8161 tctaatgaag tagaagaact ggaacgctgt ttattaggaa aaattaatat tgcgggtgct
     8221 tatgaactgc tgacgggaga gaaattacaa actgttgaag cggataacaa tttgtcgaat
     8281 aatatcataa aatcggcggt aattccgatg acagtgacaa cctcggttat cccatctaat
     8341 acaaccgaga tggcaactca gggaataact ataacccccc caacagcagc gacaatagaa
     8401 ctggcgatcg ctcctagcaa tgcttcagaa aatgtttcct caaacggatt aatacaaaat
     8461 aatcaaacaa ataacagatt gttctttaat agtgtcgttc ctagtcaacg gtcaaacctg
     8521 gtttatgtgt taggaaattt aggttatgat ttcggcacag aagcgcgtcg ggacaccttt
     8581 aaacaattaa tgccaagcat catgatcgat aatcttgaat taccatccaa tccctacgat
     8641 gcgcggcaaa tggtagatta tttagaaagt aatctatcag aaactaagtc tttaatttgg
     8701 acagtgaatt tagaactaac tcccatctat gctattaaac cagtgggagc ttttgctgct
     8761 gatatttatc aagtcatgca atatatgtta gcgggtcaaa tattgccaga agctgacgaa
     8821 gattatgttg aaagagtgag tattccgggt atgctgaccg aggaaactgt gaaacttttt
     8881 tccggtcaaa ttattcctgt cctcaaagtc aatagtcccc ggggaatgta tggctggaaa
     8941 gttaatactt tgattgaatc agcgatagaa actgttagcg cggaacatga aatcgccgat
     9001 gaagcgagga tgcggcgttc tctgaccagt ttcttgaatc gcatttatta tgacttacga
     9061 aatttaggac aaatagcacg cgatcgcgcc ttaaattttg cggctactaa cgcttttcaa
     9121 gctgtacaaa ccttctccca agcagtagca atgggaatgg aattacacag tattgaagta
     9181 gaaaaaagcc ctttttgtcg ctatggtagt gaatgttggg atgtgaaatt aaaattcttt
     9241 gatcccgaaa atggtctgcg ggccaaacga gtttttcgct ttaccattga tgttagcgat
     9301 cgcactccag taagcgtagg agaa
//
