LOCUS       BGC0001168              4582 bp    DNA     linear   CON 22-MAR-2015
DEFINITION  Streptomyces lividans 1326 chromosome, whole genome shotgun
            sequence.
ACCESSION   BGC0001168
VERSION     BGC0001168
KEYWORDS    .
SOURCE      Streptomyces lividans 1326
  ORGANISM  Streptomyces lividans 1326
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     Source DNA and bacteria available from the John Innes Center,
            Norwich Research Park, Norwich, NR4 7UH, UK.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 23:36:03
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4582
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4582
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:2967]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..2967
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..4582
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4582
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001168"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4582
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..2967
                     /locus_tag="SLI_0883"
     CDS             1..2967
                     /NRPS_PKS="Domain: AMP-binding (3-391). E-value: 3.8e-98.
                     Score: 320.5. Matches aSDomain:
                     nrpspksdomains_SLI_0883_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (503-576). E-value: 2.2e-16. Score:
                     51.7. Matches aSDomain: nrpspksdomains_SLI_0883_PCP.1"
                     /NRPS_PKS="Domain: TD (616-859). E-value: 3e-83. Score:
                     270.6. Matches aSDomain: nrpspksdomains_SLI_0883_TD.1"
                     /codon_start=1
                     /db_xref="GO:0004467"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: NAD_binding_4"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     355.1; E-value: 8.4e-108)"
                     /gene_kind="biosynthetic"
                     /locus_tag="SLI_0883"
                     /product="Adenylation and reductase domains containing
                     protein"
                     /protein_id="EOY45602.1"
                     /sec_met_domain="AMP-binding (E-value: 8.2e-115, bitscore:
                     371.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.7e-13, bitscore:
                     38.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 2.6e-64, bitscore:
                     204.4, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEHDGRRIDYTELDERANRLAHRLQSMGVIRGSVVMTHLGRSIDL
                     IVSFLATLKAGAVYLPVEPGAPDDRIATYIKETRCTTVVTHSDLEHRFSGFEVRTVSVD
                     GSSAMTHDSRSASRPPEVAVGGSDTAYIVYTSGSSGTPKGVQVSHSSLNYLCRHINEVY
                     GIGPDDRVLQYAALSFDTSIEQILVALLNGATLVLPEELWAPSELSARIASLGISVMDL
                     TPPYWRAFLSELEHSPTELPIRLTIVGGSAVHAADCRTALRLMPYSRLVNAYGLTETTI
                     TSCTMEVTPELLPSEGAAPIGRPLPGTTVLILDQDMRPVPPQQVGELYIGGPGVARGYL
                     AEEASNKDRFVSLATDVGGATRFYRTGDLGRWTAEGNLHITGRADRQVKVRGYRVEPAE
                     IEATLSAHPLIDDVAVKPYSVRDELQLAAYYTASRVQAHFQSRELRDFISGRLPTFMVP
                     TAFVRLDTMPTTAHGKPDLSALPDPARGSAMGPAADREAPATTVVERAVAGLWCQVLDV
                     ESVAPDDNFFDLGGDSIAAAQLLSKVRASLGILITQVRPLIRLLLDDATLRSFASAVES
                     ARAGTLDTADASPTDFAAEAAVGVPIQQRPTTTDWADPAHVFLTGATGFLGIHLLRELL
                     TTTGATVHCLVRASDTKDAKERITANATRYLADPLEEYWAEGRIAMVPGDLSKPRLGLT
                     EESFDHYAEVVDVIHHPGGLVNFIYPYSHMRQTNVEGTREIIRLAARHRNIPVHYVSTM
                     AVLSGFGTAGTRHVTEDTPLAHADHLSVGYVESKWVAEALLQNAAAQGLPVAIYRAADI
                     SGDRTTGAWNTATEMCAMKRFIVDTGTSPIAELPLDYTPVDRYAAALLHIAAGRLPAGE
                     VYHLTNPGKVNVALLAERLRARGYTIRDVPWDEWLERIVTTAVEEPDHPMTPFAPLFID
                     RCSTGTMSVAEMYLETTFPTFSQDNVTAALRGSGIEIPPVDGDMLDRYIDYLTSIDFL"
     aSDomain        10..1173
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SLI_0883_AMP-binding.1"
                     /evalue="3.80E-98"
                     /label="SLI_0883_AMP-binding.1"
                     /locus_tag="SLI_0883"
                     /protein_end="391"
                     /protein_start="3"
                     /score="320.5"
                     /specificity="consensus: X"
                     /tool="antismash"
                     /translation="DGRRIDYTELDERANRLAHRLQSMGVIRGSVVMTHLGRSIDLIVS
                     FLATLKAGAVYLPVEPGAPDDRIATYIKETRCTTVVTHSDLEHRFSGFEVRTVSVDGSS
                     AMTHDSRSASRPPEVAVGGSDTAYIVYTSGSSGTPKGVQVSHSSLNYLCRHINEVYGIG
                     PDDRVLQYAALSFDTSIEQILVALLNGATLVLPEELWAPSELSARIASLGISVMDLTPP
                     YWRAFLSELEHSPTELPIRLTIVGGSAVHAADCRTALRLMPYSRLVNAYGLTETTITSC
                     TMEVTPELLPSEGAAPIGRPLPGTTVLILDQDMRPVPPQQVGELYIGGPGVARGYLAEE
                     ASNKDRFVSLATDVGGATRFYRTGDLGRWTAEGNLHITGRADRQVKVR"
     aSModule        10..2577
                     /complete
                     /domains="nrpspksdomains_SLI_0883_AMP-binding.1"
                     /domains="nrpspksdomains_SLI_0883_PCP.1"
                     /domains="nrpspksdomains_SLI_0883_TD.1"
                     /final_module
                     /locus_tags="SLI_0883"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       148..186
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SLI_0883_0001"
                     /evalue="1.00E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="SLI_0883"
                     /protein_end="62"
                     /protein_start="49"
                     /score="12.6"
                     /tool="antismash"
                     /translation="LATLKAGAVYLPV"
     CDS_motif       388..447
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SLI_0883_0002"
                     /evalue="2.60E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="SLI_0883"
                     /protein_end="149"
                     /protein_start="129"
                     /score="29.1"
                     /tool="antismash"
                     /translation="AYIVYTSGSSGTPKGVQVSH"
     misc_feature    721..723
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       961..1050
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SLI_0883_0003"
                     /evalue="7.90E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="SLI_0883"
                     /protein_end="350"
                     /protein_start="320"
                     /score="37.4"
                     /tool="antismash"
                     /translation="PPQQVGELYIGGPGVARGYLAEEASNKDRF"
     CDS_motif       1141..1203
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SLI_0883_0004"
                     /evalue="5.70E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="SLI_0883"
                     /protein_end="401"
                     /protein_start="380"
                     /score="34.4"
                     /tool="antismash"
                     /translation="TGRADRQVKVRGYRVEPAEIE"
     misc_feature    1330..1332
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        1510..1728
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SLI_0883_PCP.1"
                     /evalue="2.20E-16"
                     /label="SLI_0883_PCP.1"
                     /locus_tag="SLI_0883"
                     /protein_end="576"
                     /protein_start="503"
                     /score="51.7"
                     /tool="antismash"
                     /translation="ERAVAGLWCQVLDVESVAPDDNFFDLGGDSIAAAQLLSKVRASLG
                     ILITQVRPLIRLLLDDATLRSFASAVES"
     aSDomain        1849..2577
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SLI_0883_TD.1"
                     /evalue="3.00E-83"
                     /label="SLI_0883_TD.1"
                     /locus_tag="SLI_0883"
                     /protein_end="859"
                     /protein_start="616"
                     /score="270.6"
                     /tool="antismash"
                     /translation="VFLTGATGFLGIHLLRELLTTTGATVHCLVRASDTKDAKERITAN
                     ATRYLADPLEEYWAEGRIAMVPGDLSKPRLGLTEESFDHYAEVVDVIHHPGGLVNFIYP
                     YSHMRQTNVEGTREIIRLAARHRNIPVHYVSTMAVLSGFGTAGTRHVTEDTPLAHADHL
                     SVGYVESKWVAEALLQNAAAQGLPVAIYRAADISGDRTTGAWNTATEMCAMKRFIVDTG
                     TSPIAELPLDYTPVDRYAAAL"
     gene            2990..3772
                     /locus_tag="SLI_0884"
     CDS             2990..3772
                     /EC_number="2.3.2.6"
                     /codon_start=1
                     /db_xref="GO:0008914"
                     /locus_tag="SLI_0884"
                     /product="Leucyl/Phenylalanil-tRNA-Protein transferase"
                     /protein_id="EOY45603.1"
                     /transl_table=11
                     /translation="MIRTQDRAESLWESLNVDAGPAAQPVALGGDLEPATVLAAYRSGL
                     YPFPVDTVEAAIVNELTYDADVQARRIHVVPGRDDPFTLSWWSPDPRPVIHVDQARIQR
                     SLRQQLRNRADWTTTADTCFADVVRRCRVGRAQRWLTDDLMHSLSLLHEQGHAHSVEVW
                     DRDELIGGVFGIRVGAVFSADSQFTLRSGAGKTAVADLTRRFAEAGGVAVDVQRESDHA
                     RLLGARPIPRSHYLDLLALPTAPQPLATRKLPARRLTE"
     gene            3800..4582
                     /locus_tag="SLI_0885"
     CDS             3800..4582
                     /codon_start=1
                     /locus_tag="SLI_0885"
                     /product="N-Acyl transferase"
                     /protein_id="EOY45604.1"
                     /transl_table=11
                     /translation="MPLTSTDLLVVSIAERPDLEEAMISMESSWPAYIRPDPLLVDWAF
                     DRHPEHQLVVLDEQGHVVARAAGVGIAWDGDPGRLPDAGWDAALRQCLTDTYADRRLTA
                     LCALEIAVAPGHQSRNLSARTLRALTDHARRHGFLDLIGPVRPSLKHERPDLPMAEYIK
                     LLRDDGLPQDPWLRIHVRAGGEVLKVCPASMTISAGLNEWREWTGLPLDTSGPITVPGA
                     LAPVTVSLEHDYAVYVEPNVWVRHRLTDKRPTDKENSV"
ORIGIN
        1 gtggagcacg acggtcgccg catcgactac acggaactgg atgagcgcgc caaccggctc
       61 gcgcaccggc tccagagcat gggagtcatc cgcggctccg tggtcatgac gcacctcggg
      121 cgctccatcg acctgatcgt gtcattcctg gcgaccctca aggctggggc ggtctatctg
      181 ccggtggagc ctggcgcccc ggatgatcgg atcgccactt acatcaagga gacccgctgc
      241 accaccgtcg tgacccactc ggatctcgag caccgcttct ccggtttcga ggtacggacc
      301 gtctccgtgg acggcagcag cgcgatgacg cacgacagtc ggtcggcctc ccgcccgccc
      361 gaggtcgccg tcgggggctc cgacaccgcg tacatcgtct acacctcggg ctcttcaggc
      421 acgcccaagg gtgtccaggt cagccattca tcgctcaact acctgtgccg gcacatcaat
      481 gaggtgtatg gcatcggccc ggacgatcgg gtcctgcagt acgccgcgct gtccttcgac
      541 acctccatcg agcaaatact ggtcgcgctc ctcaacggag ccacgctcgt gctgccggag
      601 gagctctggg ctccgagcga gctgtccgcc cgaattgcct cgctcggcat ctctgtcatg
      661 gacctgacgc cgccgtactg gcgcgccttc ctctccgaac tcgagcactc gccgactgag
      721 ttaccgatcc ggctcacgat cgtcggcggc agtgcggtgc atgccgccga ctgccgcacg
      781 gcgctccgcc tgatgccgta ctcccggctc gtcaacgcct acgggctcac ggaaacgacc
      841 atcacttcgt gcacgatgga ggtcaccccg gagctgcttc cctctgaagg cgccgcacct
      901 atcgggcgcc cgctgccggg cactaccgtc ctcatactcg atcaggacat gcggccggtc
      961 ccaccccagc aggtcgggga gctgtacatc ggcgggccag gcgtcgcccg gggctatctc
     1021 gccgaagagg catcgaacaa ggaccgcttc gtgtcgctgg cgacggacgt gggcggtgcg
     1081 acgcgcttct atcgcaccgg cgatctgggt cggtggacag cggagggaaa cctccacatc
     1141 accggacggg ccgaccgcca ggtcaaggtc cgtggctacc gagtggagcc tgccgagatc
     1201 gaggccacgc tgtcggccca cccgttgatc gacgacgtcg cggtgaagcc gtacagcgtc
     1261 cgggacgaac tgcagctcgc cgcctactac accgcaagcc gcgtccaagc tcacttccaa
     1321 tcacgggagt tacgcgactt catctctggt cggctgccca ccttcatggt ccccacagcc
     1381 ttcgtccgcc tcgacaccat gccgacgacg gcacacggca aacccgatct ctccgcactg
     1441 cccgacccgg cccgaggttc cgccatgggg ccagccgcag accgtgaggc tccggcgacc
     1501 accgtggtcg aacgcgctgt cgccggcctg tggtgccagg tgctcgacgt cgagtcggtt
     1561 gcacccgacg acaacttctt cgacctgggc ggcgattcga tcgccgcagc ccagttgctc
     1621 tcaaaagtac gggcctcgct cggcatcctg atcacccagg tccgtccgct catccggctc
     1681 ctcctcgacg acgcgaccct gcgcagcttc gcctctgcgg tggagtcggc ccgcgcaggg
     1741 acactcgaca ccgccgacgc ctctcccacc gacttcgcag ccgaagcggc tgttggggtg
     1801 cccatccagc aacggcctac gaccactgac tgggctgacc cagcccacgt gttcctaacc
     1861 ggcgcgaccg gattcctcgg aatccaccta ctccgcgaac tcctcacgac cacgggcgcc
     1921 acggtgcatt gcctggtccg agcctcggac acaaaggatg cgaaggagcg catcacagcc
     1981 aacgctacgc gctacctggc cgacccactg gaggagtact gggccgaggg ccggatcgcg
     2041 atggtccccg gagacctgag caagccccgt cttggcttga ccgaagagag cttcgatcac
     2101 tacgccgagg tcgtcgacgt gatccaccac ccgggcgggc tggtcaactt catctatccg
     2161 tactcgcaca tgcggcagac gaacgtcgaa ggcacccgcg aaatcattcg actggccgcc
     2221 cgtcaccgga acattccggt ccactacgtc tccaccatgg cggtcctctc gggcttcggc
     2281 accgccggta cccggcacgt caccgaagac acgcccctgg cccatgccga ccacctctcc
     2341 gtcgggtacg tggaaagcaa atgggtcgcc gaggcgctac tgcagaacgc cgctgcacaa
     2401 ggcctgccgg tcgccatcta ccgcgctgcc gacatctcag gcgatcggac aaccggcgcc
     2461 tggaacaccg cgaccgagat gtgcgcgatg aagcgcttca tcgtcgacac cggcacctcg
     2521 ccgatcgccg agctccccct ggactacacg ccagtggacc gctacgcggc cgctttgctg
     2581 cacatcgccg ccggccgact gcccgcgggg gaggtgtacc acctgaccaa ccccggcaag
     2641 gtgaacgtcg cgctcctcgc agaacgcctt cgtgcccgcg gctacacgat ccgcgacgtc
     2701 ccctgggacg agtggctcga gcgcatcgtc accactgccg tggaagagcc ggaccacccc
     2761 atgaccccgt tcgcaccgct cttcatcgat cggtgctcca ccggcaccat gagcgtcgcc
     2821 gagatgtatc tcgagaccac ctttcccacc ttctcccagg acaacgtgac ggctgccttg
     2881 cggggcagcg gcatcgagat cccgcccgtc gacggcgaca tgctcgaccg ctacatcgac
     2941 tatctgacgt ccatcgactt cctgtgaacc gactctccgt gaggtgaaca tgatccgaac
     3001 gcaggatcga gccgagtccc tgtgggagtc gctgaatgtc gatgcgggac cggccgcgca
     3061 gcccgtcgct ctcggcggcg acctcgaacc cgcgaccgtc cttgccgcct accggagcgg
     3121 gctgtacccg tttccggtcg acacggtcga agccgcgatc gtcaacgaac tgacctacga
     3181 cgccgatgtc caagccaggc gcatacatgt cgtccccggc agggacgacc ccttcaccct
     3241 gtcctggtgg tctccggatc cgcgcccggt gatccacgtg gaccaggccc ggatccaacg
     3301 cagcctgcgc cagcagttgc ggaaccgagc ggactggacg accaccgccg acacctgctt
     3361 cgcggacgtg gtccgacgct gccgggtcgg ccgggcccag cgctggctga ccgacgatct
     3421 gatgcacagc ctgtctcttc tgcacgaaca aggacatgct cacagcgtcg aggtttggga
     3481 ccgcgatgaa ctcatcggtg gcgtcttcgg catcagggtg ggcgccgtct tcagcgcgga
     3541 ctcccagttc acgctccgca gcggggcagg gaagaccgcg gtcgccgacc tgacacgccg
     3601 cttcgccgag gccggcggag tcgcggtcga cgtccagcgc gagagcgacc acgccaggct
     3661 cctcggcgcc cgccccatcc ccaggtcgca ctacctcgac ctgctggcac tccccaccgc
     3721 cccacagccc ctcgccacgc gaaagctgcc cgcgaggcgg ctcaccgagt gatcacacag
     3781 acaggacccg gagtacacct tgcctctcac cagcaccgat ctgctcgtgg tcagcatcgc
     3841 cgagcggcca gaccttgaag aagccatgat ctcgatggag tcgtcgtggc cggcctacat
     3901 ccgccccgac ccgctgctcg tggactgggc cttcgaccgc cacccggagc accagctcgt
     3961 cgtgctcgac gagcaaggtc acgtcgtcgc acgggcggcg ggggtgggga tcgcctggga
     4021 cggggatccc ggtcggctgc ccgacgcggg ctgggatgcg gcactgcggc agtgcctcac
     4081 agatacgtat gcggaccggc gactcaccgc gctgtgtgcc ctggaaatcg ccgtcgcccc
     4141 cggacaccag tcacggaacc tgtccgcccg taccctgcgc gcccttaccg accacgcccg
     4201 tcgacacggg ttcctcgatc tcatcggccc ggtccgcccc agcctcaagc acgagcgccc
     4261 cgacctgccc atggccgagt acatcaagct gctgcgtgac gacgggctgc cccaggaccc
     4321 atggctccgc atccatgtca gagcgggtgg cgaggtcctg aaggtgtgtc cggcctccat
     4381 gacgatctcc gctgggctca acgaatggcg cgagtggacc ggcctccccc tggacaccag
     4441 cggtccgatc accgtgcccg gcgcactcgc cccagtgacc gtcagcctcg agcacgacta
     4501 cgcggtgtac gtcgaaccca acgtgtgggt caggcaccgc ctcaccgaca agcgccccac
     4561 cgacaaggag aactccgtat ga
//
