LOCUS       BGC0001170              8486 bp    DNA     linear   CON 16-SEP-2013
DEFINITION  Clostridium sporogenes ATCC 15579 Scfld_02_1 genomic scaffold, whole
            genome shotgun sequence.
ACCESSION   BGC0001170
VERSION     BGC0001170
KEYWORDS    .
SOURCE      Clostridium sporogenes ATCC 15579
  ORGANISM  Clostridium sporogenes ATCC 15579
            Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
COMMENT     Clostridium sporogenes (GenBank Accession Number for 16S rDNA gene:
            DQ911258*) is a member of the Firmicutes division of the domain
            bacteria and has been isolated from human feces. The sequenced
            strain was obtained from ATCC (ATCC 15579).
            We have collected 8.9X coverage in plasmid end reads and 454 reads.
            We have performed one round of automated sequence
            improvement(pre-finishing), along with manual improvement that
            includes breaking apart any mis-assembly, and making manual joins
            where possible. Manual edits also are made where the consensus
            appears to be incorrect. All low quality data on the ends of
            contigs is removed. Contigs are ordered and oriented where
            possible.
            Sequencing/Assembly: The genomic DNA was purified from liquid
            culture derived from a single bacterial colony. A hybrid sequencing
            strategy that utilized reads from both 454 GS-20 and ABI 3730xl
            sequencers was devised and implemented to generate the draft genome
            sequences. 454 reads were assembled using Newbler (454 Life
            Sciences) into 454 de novo contigs.These de novo contigs were
            converted in silico to 800 base paired reads ('superreads') with
            400 base overlaps with neighboring superreads. Finally, PCAP
            (Huang, et al, Genome Research, 13:2164, (2003)) was used to
            assemble the super-reads and the conventional 3730xl capillary
            reads.
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gutmicrobiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representativesof the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:02:34
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8486
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..183
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03602"
                     /gene_kind="biosynthetic"
                     /inference="similar to AA sequence:REFSEQ:YP_001253030.1"
                     /locus_tag="CLOSPO_01168"
                     /note="Psort location: Extracellular, score: 8.82"
                     /product="bacteriocin protoxin, streptolysin S family"
                     /protein_id="EDU38306.1"
                     /sec_met_domain="TIGR03602 (E-value: 1e-35, bitscore:
                     111.0, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDMLKFNEHVLTTTNNNNNKVTVAPGSCCCCSCCCCVSVSVGGG
                     SASTGGGAAAGQGGN"
     protocluster    1..8486
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[0:3763]"
                     /cutoff="20000"
                     /detection_rule="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /neighbourhood="10000"
                     /product="LAP"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..3763
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /neighbourhood="10000"
                     /product="LAP"
                     /protocluster_number="1"
     cand_cluster    1..8486
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /kind="single"
                     /product="LAP"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8486
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001170"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8486
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="LAP"
                     /region_number="1"
                     /rules="((((goadsporin_like or PF00881 or TIGR03605) and
                     (YcaO or TIGR03604)) or TIGR03882 or TIGR03603 or
                     TIGR03602) and not cyanobactin_synth)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..183
                     /locus_tag="CLOSPO_01168"
     gene            348..1304
                     /gene="sagB"
                     /locus_tag="CLOSPO_01169"
     CDS             348..1304
                     /codon_start=1
                     /gene="sagB"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     PF00881"
                     /gene_kind="biosynthetic"
                     /inference="similar to AA sequence:REFSEQ:YP_001389852.1"
                     /locus_tag="CLOSPO_01169"
                     /note="KEGG: tth:TTC1977 3.7e-05 glucose-1-phosphate
                     adenylyltransferase K00975; COG: COG0778 Nitroreductase;
                     Psort location: Cytoplasmic, score: 8.87"
                     /product="streptolysin associated protein SagB"
                     /protein_id="EDU38307.1"
                     /sec_met_domain="PF00881 (E-value: 5.6e-29, bitscore: 90.6,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLKNLKKQKVKKQKNTDIENRMLIHFNTQYSSISPYADSTVVKT
                     PDTVIKGSLYSKHNRFVSQEYLLNYRSNNNNLGFRTGVASFFQRDAALTSVNLNMEEEG
                     EDIIELPSFKNIRVGLSTAIKSRRSVRRYSNKFMSLNDVANILYYTQGICDEVEPYNLE
                     SNEKKIKLRANPSAGGLYPIQLYVYMKNIKDLEDGVYTYYPYSHSLKPIKVNKESLKID
                     KFAEFGTLNSENANIIVFYVYNFLKNSRKYGDGGFAYALIETGEIAQNLQLVSTALGYG
                     ACDIGGYEKQYIEELLNIDGVLKHVVHMTVVGWEQED"
     gene            1307..2389
                     /locus_tag="CLOSPO_01170"
     CDS             1307..2389
                     /EC_number="6.3.-.-"
                     /codon_start=1
                     /db_xref="InterPro:IPR009036"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03603"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cytolysin_C_RRE"
                     /gene_kind="biosynthetic"
                     /inference="protein motif:superfamily:IPR009036"
                     /inference="similar to AA sequence:REFSEQ:YP_001389853.1"
                     /locus_tag="CLOSPO_01170"
                     /product="bacteriocin biosynthesis cyclodehydratase, SagC
                     family"
                     /protein_id="EDU38308.1"
                     /sec_met_domain="Cytolysin_C_RRE (E-value: 9.5e-32,
                     bitscore: 98.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03603 (E-value: 2.4e-122, bitscore:
                     397.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENNTIYKLSNNLRIYDSGENEIRFRSGLWNYNEAVLDLSSETPN
                     FIKAFRIIINDLKNNKAFSMKYLDKHNLNDQEKNNLINVISSLSEAGMLCSENEKDNEF
                     EMSKVLLGDFRYNLNKKEGEPQKVLFISDNDYAKETAKSLSDGMNMKLDLCAKETIDNI
                     ISMDLTSNLDALEKKSNTENLKNKLKNYSSILICMGHINMNLFRNINRVSIELKKPIIM
                     SFIDGPFITILSTLPPKTGCLECFEQRILSRLEDHVLYHKFIESDFKTSEKPHKSIIPL
                     LNIMTNMLISEGFLINNFNTCKIEGRVLSIFVPTLEIQTQDLLRVPFCPACGNISKAKL
                     SEINISTRNIVDEILEDINS"
     gene            2405..3763
                     /locus_tag="CLOSPO_01171"
     CDS             2405..3763
                     /codon_start=1
                     /db_xref="InterPro:IPR003776"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     YcaO"
                     /gene_kind="biosynthetic"
                     /inference="protein motif:HMMPfam:IPR003776"
                     /inference="similar to AA sequence:REFSEQ:YP_001253033.1"
                     /locus_tag="CLOSPO_01171"
                     /product="putative YcaO-like family"
                     /protein_id="EDU38309.1"
                     /sec_met_domain="YcaO (E-value: 5e-62, bitscore: 199.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 4.3e-114, bitscore:
                     370.4, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKFYPSFNNILENFKALSGNQSGILEASCMPVVNFNSDVFLKSI
                     TGNMPDYHKQVFNKDVSMQYHIIGYGSHYEEALIKYTGESIERYSSIMGPTLLKDKIIY
                     KSHKELSKNNKVMPLKYIDVFTEKQIKKAKELNVAICDKKVKEDDIIGWIKCPSLLNKG
                     EEIYVPAKMMFVGYEEDKSKGEMNFIPSFSTGTAAHKSLKKALLNALIEYIQIDSFMIT
                     WYTKGKCSLVKIDDENVLKVLEDVNLGENSPYEIIPVYMALEECPIHNFGVFLRRKDNK
                     LPYLLFGVQGGLDASHALTRGIMEAASISYGAYFNTIYNKNMLNNVMKDDHKFLDLDSN
                     VLYYSLPSKIEEKNKLVEEFIENEINLSSLNKLNIEDVDEQIKTLLNYLKNMSEYAVYL
                     DLTPPETRDKGWYVMRVMIPELIEMCIPEFPFENHPRMKKYGGVINEHPHPMP"
     gene            3894..4376
                     /locus_tag="CLOSPO_01172"
     CDS             3894..4376
                     /codon_start=1
                     /db_xref="InterPro:IPR003675"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="protein motif:HMMPfam:IPR003675"
                     /inference="similar to AA sequence:REFSEQ:YP_001389855.1"
                     /locus_tag="CLOSPO_01172"
                     /note="COG: COG1266 Predicted metal-dependent membrane
                     protease; Psort location: CytoplasmicMembrane, score: 9.99"
                     /product="CAAX amino terminal protease family protein"
                     /protein_id="EDU38310.1"
                     /sec_met_domain="Abi (E-value: 3.4e-13, bitscore: 39.2,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGIFVISQEKFYINIDNNYYWYIIAVIFVPLTIILEIIVGYIVIG
                     ISDKILPKFKISLAFTKTNINVIILSVFIGIFEEVVYRQLWFNILFKDFKLHIVGVFII
                     TSFVYALNHIFLGKQVLFQKILAGVVYGGLFYFSGFNILIPIITHCLENTVILLKG"
     gene            4384..5112
                     /locus_tag="CLOSPO_01173"
     CDS             4384..5112
                     /codon_start=1
                     /locus_tag="CLOSPO_01173"
                     /note="KEGG: bcc:BCc_109 0.0063 nuoN; NADH dehydrogenase I
                     chain N K00329; Psort location: CytoplasmicMembrane, score:
                     9.99"
                     /product="hypothetical protein"
                     /protein_id="EDU38311.1"
                     /transl_table=11
                     /translation="MSLYFKILVFILMSMYIVLLYLPININYKKFKSIKKLCLNLGGFI
                     GISPTNCWKLIKYIHLLIIGVIPMAYFLYNGFVKSYNIYTQTNIIESFILIILSFIGVL
                     EISFFIIIVIISYLMKKDSRKEMSKVSWIKFNKRYPFHTGIVKPIIYAFFEVILYYYVM
                     LYIFNDFLELPLIYSITGIAILYCVSKLVFTKNKEQALIYGIWAFVLNFIGSCIMIYSN
                     SIILTLILYLFYSFIIAFKE"
     gene            5223..6206
                     /locus_tag="CLOSPO_01174"
     CDS             5223..6206
                     /codon_start=1
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 216.6; E-value:
                     6.3e-66)"
                     /gene_kind="transport"
                     /inference="protein motif:BlastProDom:IPR003439"
                     /inference="protein motif:HMMPfam:IPR003439"
                     /inference="protein motif:HMMSmart:IPR003593"
                     /inference="protein motif:ScanRegExp:IPR003439"
                     /inference="similar to AA sequence:REFSEQ:YP_001253036.1"
                     /locus_tag="CLOSPO_01174"
                     /note="KEGG: reh:H16_A2602 5.2e-48 ABC-type transporter,
                     ATPase component: LOSE family; COG: COG1131 ABC-type
                     multidrug transport system, ATPase component; Psort
                     location: CytoplasmicMembrane, score: 9.49"
                     /product="ABC transporter, ATP-binding protein"
                     /protein_id="EDU38312.1"
                     /transl_table=11
                     /translation="MFILHDYCRRYYKGDDLMNIVEIKNLEKKFGQNTAVNNINLAVKE
                     GQVFGFLGPNGAGKSTTINMICGLLTPTKGEIFILGKKMDNKAKDLKNNIGIVPQDIAI
                     YEDLTAYENVAFFGGLYGIRGQELKERVKETLEFVGLLDRAKDFPYKFSGGMKRRLNIA
                     CALVHQPKLIIMDEPTVGIDPQSRNHILESIKKLNERGCTIIYTSHYIDEVEQLCTDIA
                     IIDKGTIIAKGKKDELVEKYSDLNMVVINTENLTKVDIKALKSIDGVMEVNIKENTIEI
                     TNKSSDNLDDIIMYFLKNKIKIKNIENKHSDLETIFLKLTGKKLRD"
     gene            6218..7342
                     /locus_tag="CLOSPO_01175"
     CDS             6218..7342
                     /codon_start=1
                     /db_xref="InterPro:IPR013525"
                     /inference="protein motif:HMMPfam:IPR013525"
                     /inference="similar to AA sequence:REFSEQ:YP_001253037.1"
                     /locus_tag="CLOSPO_01175"
                     /note="KEGG: fal:FRAAL1581 0.0071 putative ABC transporter
                     ATP-binding protein; COG: COG0842 ABC-type multidrug
                     transport system, permease component; Psort location:
                     CytoplasmicMembrane, score: 9.99"
                     /product="ABC-2 type transporter"
                     /protein_id="EDU38313.1"
                     /transl_table=11
                     /translation="MKILNILKKEILQNLRDKKSMLLMTLFPMLLITILGTVFSGNFAS
                     TINIPEINVMYSINKDVKIDKNFKDFTKEIEKTMKVNFKETKDEKDALEKISNGKTDVY
                     IKIPNDKKIYIYENNLRAFNSQLVSTLLDAFVDKVNMTKEVASKNPMALSKIKVNTSPN
                     FVNTKTIDGKDSPRGIDYYAVTMLTMTILYGTAVGAMSIASEIKRRTYKRLICSNTSPL
                     KILFSKILASFLVIAFQSFLIYLFSKYILGTNWGNNKLALVSVVASLIFMAVSAGVCIA
                     NTIKNEGVMSIIIYMFVPILTFLGGGYVPLEPIGSKMLNSVSNISPLKWSNDAIFKIIF
                     GNNLSIFGITMLINIGAGVLFLLIAILFPRKDVI"
     gene            7344..8486
                     /locus_tag="CLOSPO_01176"
     CDS             7344..8486
                     /codon_start=1
                     /db_xref="InterPro:IPR013525"
                     /inference="protein motif:HMMPfam:IPR013525"
                     /inference="similar to AA sequence:REFSEQ:YP_001253038.1"
                     /locus_tag="CLOSPO_01176"
                     /note="COG: COG0842 ABC-type multidrug transport system,
                     permease component; Psort location: CytoplasmicMembrane,
                     score: 9.99"
                     /product="ABC-2 type transporter"
                     /protein_id="EDU38314.1"
                     /transl_table=11
                     /translation="MKQVFLLTKSILKRTFRKKSNIITFILLPLLAVFIALFLNASGTS
                     YSKIGVVDLNESYLSKDMIKSIDSKKNFVVVKVSKNNMNKQLLDKKVDCVAVIPKDFHK
                     EIIEGNFKKLDIISVRGEDITSWIKNYLNYYIDNLTNISLVSNKDEKTFKDMYDGFKKQ
                     DLKLNYKAIGDKTKSKSVTKQSIGLLLVFMLIASSTASSFILKDKANKTYYRIFCSNIS
                     KSKYIIANVIANFIIFLIQIFVILFMSLYVLKLNFYIDTKIMFIILCSFGFVSIGFGII
                     IAAFSKSLNQSSYLSNILITPTCMLSGCFWPLDMMPNFMQKLANIFPQTWILKTIDSLQ
                     QGKSFNEVVLYIGIVILFAVSFFIIGILKLTKDENLKSIF"
ORIGIN
        1 gtgaaagata tgttaaaatt taatgaacac gtactgacaa ctactaataa caataataat
       61 aaggttactg tggctccagg aagttgttgc tgctgctctt gttgctgttg cgttagtgtt
      121 agtgtaggtg gcggttcagc atcaacaggt ggcggagcag ctgctggaca aggtggaaat
      181 taataatttt atcttgtaca agcatctttc acaattcaat tatttaataa taatttaaac
      241 ccaactagcc aggtgattat aatttaatat tattttatca cctggctaat atcatttaaa
      301 tatttaattt atatttaaga tttaaaaata tacacaagga gctaaaaatg ttactaaaaa
      361 acttgaagaa acaaaaagta aaaaagcaaa aaaatactga tatagaaaat agaatgctta
      421 ttcattttaa tactcagtat tctagtatat caccctatgc tgatagtact gtggttaaaa
      481 ccccagatac agtaataaaa gggagtttat actctaaaca taacagattt gtaagtcaag
      541 aatatctctt aaattataga tctaacaata ataatttagg atttagaaca ggagtagcaa
      601 gttttttcca aagggatgct gctttaactt ctgtaaattt aaatatggaa gaagaagggg
      661 aagatattat agaacttcca tcttttaaaa atataagagt aggtttatca acagctataa
      721 aatccagacg gagtgtaaga agatattcta ataaatttat gtctttaaat gatgtggcaa
      781 atatacttta ctatactcag gggatatgtg atgaagtaga accatataat cttgaaagta
      841 atgaaaaaaa aattaaactg cgagctaatc catccgcagg agggttatac cctatacaat
      901 tatatgtata tatgaaaaat ataaaagacc tagaggatgg cgtatataca tactatcctt
      961 attctcatag tctaaaacct attaaagtga ataaagaaag tttaaagata gataagttcg
     1021 cagaatttgg aactttgaat tctgaaaatg caaatataat tgttttttat gtatataatt
     1081 tcttgaaaaa ttcaagaaaa tatggggatg gaggttttgc ttatgcatta attgaaacag
     1141 gagaaattgc tcaaaatctg caactagtgt ctacggcttt aggttatgga gcttgtgata
     1201 tagggggata tgaaaaacaa tacatagaag agcttttaaa tatagatgga gtattaaaac
     1261 atgtagtaca tatgactgtt gtaggttggg aacaggagga ttaattatgg aaaataatac
     1321 tatatacaaa ttaagcaata atttaagaat atatgatagc ggtgaaaatg aaataagatt
     1381 tagaagtgga ttgtggaact ataatgaagc agttttagac ttaagctcag agacaccaaa
     1441 ttttataaaa gcttttagaa ttattataaa tgatttgaaa aataataaag ctttttcaat
     1501 gaaatattta gataaacata atctaaatga ccaagagaag aataatttaa ttaatgttat
     1561 aagctcttta agtgaagcag gtatgttatg tagtgaaaat gaaaaagaca atgaatttga
     1621 aatgtcaaag gtattattag gggattttag atataatctt aataaaaaag agggggagcc
     1681 ccaaaaagta ttatttatat cagataatga ttatgctaaa gaaactgcta aaagtttatc
     1741 tgatggaatg aatatgaaat tagatttatg tgcaaaagaa actatagata atataatttc
     1801 tatggattta acttctaatt tggatgcttt agaaaaaaaa tcaaacacag agaatctaaa
     1861 aaataaatta aagaattact ctagcatatt aatatgtatg ggtcacatta atatgaattt
     1921 atttagaaat ataaatagag tatctataga acttaaaaaa cctattatta tgtcctttat
     1981 tgatggccca tttataacta ttttaagtac attaccacct aaaacgggat gtctagagtg
     2041 ttttgaacaa agaatactat caagacttga agatcatgtt ttatatcata aatttataga
     2101 aagtgacttc aaaactagtg aaaaacctca taaatccata attcctttat taaatattat
     2161 gactaatatg ttgatatctg aagggttttt aataaataat tttaatactt gtaaaattga
     2221 aggtagggta cttagtatat ttgtacctac tttagaaata caaactcaag atttattaag
     2281 agtaccattt tgtcctgctt gtggaaatat atcaaaggct aagttaagtg aaataaatat
     2341 atccactaga aatattgtgg atgaaatttt agaagatatt aattcttaac taggaggctt
     2401 tgagatgata aaattttacc caagttttaa taatatatta gagaacttta aagctttatc
     2461 aggaaatcaa agtggtatat tagaagcatc ctgtatgcct gtagttaatt ttaatagtga
     2521 tgtgtttttg aaaagtataa caggaaacat gccagattat cataaacaag tatttaataa
     2581 ggatgtatct atgcaatatc acataatagg ttatggatcc cattatgagg aagctttaat
     2641 taaatatacc ggtgaaagta ttgaaaggta ttctagtatt atgggaccta cactattaaa
     2701 ggataagatt atatataaaa gtcataagga attaagcaaa aataataaag tgatgccttt
     2761 aaaatatata gatgtattta cagaaaaaca aataaaaaaa gctaaagaat taaatgtggc
     2821 tatttgtgat aaaaaagtaa aagaagatga cataatagga tggattaaat gcccttcttt
     2881 attaaacaaa ggtgaagaaa tatatgtacc agcaaaaatg atgtttgtag gctatgaaga
     2941 ggataaaagt aaaggagaaa tgaattttat accatccttt agtacaggta ctgcagctca
     3001 taaaagtctt aaaaaagctc ttttaaatgc tctaattgaa tatattcaaa ttgattcttt
     3061 tatgattacc tggtatacaa aagggaaatg ttctttagtt aaaatagatg atgaaaatgt
     3121 gcttaaagtt ttagaagatg taaatctagg agaaaattct ccctatgaaa taatacctgt
     3181 atatatggct ttagaggaat gccctataca taattttgga gtttttttaa gaagaaagga
     3241 taataaactt ccttatttac tatttggtgt acaaggagga ttagatgctt cgcatgctct
     3301 aactagagga ataatggaag cagcttcaat atcctatgga gcatatttta atacgattta
     3361 taataaaaat atgctaaata atgtgatgaa agatgatcat aaatttttgg atttagattc
     3421 aaatgtgcta tattattctc ttccaagcaa aattgaagaa aaaaataaac tagtagaaga
     3481 gtttatagag aatgaaatta atttaagttc tttaaataaa ttaaatatag aagatgtaga
     3541 tgagcagata aagactttat taaattattt aaaaaatatg agtgaatatg cagtatattt
     3601 agatttaacg ccgccagaaa ctagggacaa gggttggtat gttatgagag ttatgattcc
     3661 agaattaatt gaaatgtgta taccagagtt tccctttgaa aatcatccaa gaatgaaaaa
     3721 atatgggggg gtaattaatg aacatccgca cccaatgcct taatttatta atactattag
     3781 ttacctttaa tccgatagta ttaactcagt tatttttaaa taatgaaaaa tcctacaatg
     3841 aatcattgaa aaaaagcctt aaaatactag ctataattta tataataata ctcatgggga
     3901 tatttgttat ttcccaggaa aaattttata taaatataga taataattac tattggtata
     3961 ttattgctgt tatatttgta ccattaacta taattttgga aataattgta ggatatattg
     4021 tgataggtat ttctgataag atccttccca aatttaaaat atccttggca tttacaaaaa
     4081 ccaatattaa tgtgattata ttatctgttt ttataggtat ttttgaagaa gttgtatata
     4141 gacaattatg gtttaatatt ttatttaaag attttaagtt acatatagtg ggagttttta
     4201 ttattacatc ctttgtatat gccttaaatc atatattttt aggtaaacag gtattatttc
     4261 aaaaaatact agcaggggtt gtttatggag gattatttta ttttagtgga tttaatatat
     4321 taattcctat aataactcat tgcttagaaa atacagtcat tctcttgaaa ggttgataag
     4381 tttatgagtt tatattttaa aattttagta tttattttaa tgagtatgta catagtactg
     4441 ctttatttac ctattaatat taattataaa aaatttaaat ctataaaaaa attatgttta
     4501 aatttaggtg gatttatagg aatatcacca actaattgtt ggaaattaat aaaatatata
     4561 catttattaa ttataggtgt aatacccatg gcatactttt tatacaacgg ttttgttaaa
     4621 agttataaca tatataccca aacaaatata attgagtctt ttattttgat aatattgtct
     4681 tttatagggg ttttagaaat aagctttttt ataattatag ttattatttc atatttaatg
     4741 aaaaaggata gtagaaaaga aatgagtaag gtatcttgga taaaatttaa taaaagatat
     4801 ccatttcata caggaatagt taaaccaata atatatgctt tctttgaagt gattctttat
     4861 tattatgtaa tgttatatat atttaatgat tttttagaat tacctcttat atattctatt
     4921 actggtatag caatattata ttgcgtaagt aaattagttt ttacaaaaaa caaagagcaa
     4981 gccttaatat atggaatttg ggcctttgtt ttaaatttta ttgggtcttg tataatgatt
     5041 tacagtaatt ccatcattct tactcttatt ctatatttat tttattcatt tataatagct
     5101 tttaaagaat aaaccttaaa cattatactt ttagagaata gtaaatcgta tattttatac
     5161 aagaaatgtt atatgaaaaa atatacacta tatcctttta tcatatgtat aaaatattat
     5221 aaatgtttat tttgcacgat tactgtagga gatattacaa aggagatgat cttatgaata
     5281 ttgtagaaat aaaaaatctt gaaaaaaaat ttggacaaaa tactgccgtt aataacataa
     5341 atttagctgt taaagaggga caagtctttg gatttctagg gccaaatggg gcaggaaaaa
     5401 gtactactat aaatatgatt tgtggacttt taactccaac taaaggtgaa atatttatct
     5461 taggtaagaa aatggacaat aaagctaagg atttaaaaaa taatattggc attgttcctc
     5521 aagatatagc tatatatgag gatttaactg cctatgaaaa tgtagctttc tttggaggtc
     5581 tttatggtat aagaggacaa gaactaaaag aaagagtaaa ggaaacctta gaatttgttg
     5641 gtttattaga tagagcaaag gattttccat ataaattttc tggtggtatg aagcgtagat
     5701 taaacattgc ttgcgctcta gtacaccaac ctaaacttat tataatggac gagcctaccg
     5761 taggtattga tccccaatca agaaatcata tcttagagtc tataaaaaag ctaaatgaaa
     5821 gagggtgtac aataatatat acaagtcatt atatagatga agtagaacaa ttatgtacag
     5881 atatagctat tattgataag ggaactatca ttgctaaggg taaaaaagat gaactagtag
     5941 aaaaatattc ggacttaaat atggttgtaa ttaatactga aaatttaact aaagtagata
     6001 taaaagcatt aaaatctata gatggcgtaa tggaggttaa tattaaagaa aatactattg
     6061 aaattacaaa caagtcatct gataatttgg atgatataat catgtacttt ttaaagaata
     6121 aaattaaaat aaaaaatata gaaaataaac atagtgattt agaaacaata ttccttaaat
     6181 taactggcaa aaaattaaga gattagaggt gaactctatg aaaatattaa atatattaaa
     6241 aaaagaaata cttcaaaatt tgagagataa aaaatctatg cttttaatga ccttgtttcc
     6301 tatgctttta attactatac taggaacggt tttttcagga aattttgcat ctacaattaa
     6361 tatcccagag attaatgtta tgtattctat aaataaggat gtaaaaatag ataaaaactt
     6421 taaggatttt actaaagaaa ttgaaaaaac tatgaaagtt aattttaaag aaactaaaga
     6481 tgaaaaggat gctttagaaa aaataagtaa tggtaaaact gatgtatata taaagattcc
     6541 aaatgataaa aaaatatata tttatgaaaa taatttgcga gcatttaact cccaattagt
     6601 tagtactttg ttggatgctt ttgtggataa agtgaatatg acaaaagaag tggcatcaaa
     6661 gaatccaatg gctttgtcta aaataaaagt taatacctca cctaattttg taaatacaaa
     6721 aactatagat ggtaaagatt ctccaagggg aatagattat tatgctgtta ctatgcttac
     6781 tatgactatt ttatatggaa cagcagtagg agctatgagt attgctagtg aaataaaaag
     6841 aagaacttat aaaagattaa tctgtagtaa tacctcacct ttaaaaatat tattttcaaa
     6901 aatattagct tcttttttgg ttatagcctt tcaaagtttt ttaatatatt tgtttagtaa
     6961 atatatttta gggacgaatt gggggaataa taaattagct ttggtttctg tggtagcatc
     7021 tttaatattt atggcagtat ctgcaggggt gtgtatagct aatactataa aaaatgaagg
     7081 ggttatgtct ataattatat atatgttcgt acctatttta actttcctcg gaggaggtta
     7141 tgtaccttta gaaccaatag gaagcaaaat gttaaattct gtatcaaata tatcaccact
     7201 taaatggagc aatgatgcta tatttaaaat tatttttggc aataatctaa gtatatttgg
     7261 cataactatg ctaattaata taggggctgg agttttattt ttattaatag ctatattatt
     7321 cccaagaaag gatgtgatat aaaatgaaac aagtattttt attaaccaaa agtattttaa
     7381 aaagaacctt tagaaaaaag tctaatataa taacctttat attacttcct ttattggctg
     7441 tatttatagc tttattttta aatgcctctg gaacttctta tagtaaaata ggagtggtag
     7501 atttaaatga atcttattta tctaaagata tgataaaatc aatagattct aagaaaaatt
     7561 ttgttgtagt taaagtttca aagaataata tgaataaaca attattggat aaaaaggtag
     7621 attgtgtagc agttattcct aaggattttc ataaggaaat aatagagggg aattttaaaa
     7681 aattagatat aatatctgtt aggggagaag atattacttc ttggataaaa aattacttaa
     7741 attattatat agataatctt actaatataa gcttagtttc taataaggat gaaaaaacat
     7801 ttaaagatat gtatgatgga tttaaaaaac aagatttaaa gcttaattat aaggctatag
     7861 gagataaaac taaaagtaaa tccgtaacaa agcaaagcat aggtttatta cttgtattta
     7921 tgcttatagc ttctagtaca gcttcatctt ttatattaaa ggataaagct aataaaacat
     7981 attatagaat attttgttct aatattagta aaagtaaata tataatagct aatgtaatag
     8041 ctaattttat aattttttta atacagatat ttgtaatttt gtttatgtca ttatatgttc
     8101 tgaaacttaa tttttatata gatacaaaga taatgtttat aatattatgt tcctttggct
     8161 ttgtatctat tggttttgga ataattattg cagctttttc taaaagtctt aatcaatcat
     8221 cctatttatc aaatatacta ataactccaa cgtgtatgct atcaggatgc ttttggcctt
     8281 tagatatgat gccaaacttt atgcaaaaat tagctaatat atttcctcag acctggatac
     8341 ttaaaactat agattctctt caacaaggga agagttttaa tgaggttgtt ttatatattg
     8401 gtatagtaat tttatttgca gtaagctttt ttattatagg aatattaaaa ttaactaagg
     8461 atgaaaactt aaaaagtatt ttttaa
//
