Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001171.1:
Cluster 1RiPP15892

BGC0001171, listeriolysin S biosynthetic gene cluster from Listeria monocytogenes. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Listeriolysin S
Molecular activity: Cytotoxic

Class-specific details

Biosynthetic class(es):
RiPP subclass:
LAP (linear)
Peptides in this cluster:
RiPP core peptide sequence(s): CCSCSCSTCTCTCTCASSAATKM
Leader peptide length: 26
Follower peptide length: -1
Peptidase(s) involved in precursor cleavage: lmof2365_1119

Gene cluster description

listeriolysin S (BGC0001171). Gene Cluster 1. Biosynthetic class = RiPP. GenBank AE017262, positions 1126697-1132588. Click on genes for more information.


biosynthetic genes
transport-related genes
regulatory genes
other genes

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Sequence-based prediction
Comments:The genes within the llsGHXBCDE operon section should also contain an unannotated precursor peptide gene, llsA.
Contact for this cluster:Professor Douglas Mitchell (University of Illinois at Urbana-Champaign)

Literature references

1. Lee SW et al. (2008) Discovery of a widely distributed toxin biosynthetic gene cluster. Proc Natl Acad Sci U S A 105(15):5879-84. doi:
2. Cotter PD et al. (2008) Listeriolysin S, a novel peptide haemolysin associated with a subset of lineage I Listeria monocytogenes. PLoS Pathog 4(9):e1000144. doi: 10.1371/journal.ppat.1000144.
3. Mitchell DA et al. (2009) Structural and functional dissection of the heterocyclic peptide cytotoxin streptolysin S. J Biol Chem 284(19):13004-12. doi: 10.1074/jbc.M900802200. Epub 2009
4. Clayton EM et al. (2011) Real-time PCR assay to differentiate Listeriolysin S-positive and -negative strains of Listeria monocytogenes. Appl Environ Microbiol 77(1):163-71. doi: 10.1128/AEM.01673-10. Epub
5. Molloy EM et al. (2011) Streptolysin S-like virulence factors: the continuing sagA. Nat Rev Microbiol 9(9):670-81. doi: 10.1038/nrmicro2624.
6. Clayton EM et al. (2014) Atypical Listeria innocua strains possess an intact LIPI-3. BMC Microbiol 14:58. doi: 10.1186/1471-2180-14-58.