BGC0001172 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001172 (Streptomyces sp. CNH287)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	42,435	High	chlorizidine A	NRPS:Type I+PKS:Type I

BGC0001172 - Region 1 - T1PKS

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Location: 1 - 42,435 nt. (total: 42,435 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 42,436
Label		BGC0001172

Tailoring functions

Oxidoreductases: dehydrogenase oxidase

clz15: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

clz3: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

clz9: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Transferases: PPTase acylase halogenase kinase

clz1: glycerol kinase

smcogs: SMCOG1210: glycerol kinase

clz17: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

clz5: flavin-dependent pyrrole halogenase

halogenases: flavin-dependent pyrrole halogenase
smcogs: SMCOG1119: halogenase

clz6: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

clz7: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

clz8: ambiguous flavin-dependent halogenase

halogenases: ambiguous flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

Hydrolases: esterase

clz21: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

clz11: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

clz14: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001172.5	0.99	NRPS, PKS	chlorizidine A	Streptomyces sp. CNH-287
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001172.5	0.99	NRPS, PKS	chlorizidine A	Streptomyces sp. CNH-287
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 39080:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ccmal) + (mal)
Non-colinear ordering:: clz6 + clz7

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

clz6: pk - mmal

PKS_AT (558..853): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 70.8%; CE-Malonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 139.2
Isobutyryl-CoA: 121.7
Malonyl-CoA: 116.4
Methoxymalonyl-CoA: 116.3
Ethylmalonyl-CoA: 99.6
Propionyl-CoA: 69.9
Acetyl-CoA: 65.7
Benzoyl-CoA: 60.2
2-Methylbutyryl-CoA: 55.3
CHC-CoA: 52.2
3-Methylbutyryl-CoA: 51.0
trans-1,2-CPDA: 48.5
fatty_acid: 44.3
inactive: 29.2

PKS_AT (1645..1939): mmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%; 2-Methylbutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 138.4
Methoxymalonyl-CoA: 116.5
Ethylmalonyl-CoA: 110.1
Isobutyryl-CoA: 102.9
Malonyl-CoA: 90.1
2-Methylbutyryl-CoA: 77.1
trans-1,2-CPDA: 74.5
Benzoyl-CoA: 70.5
Propionyl-CoA: 64.2
CHC-CoA: 64.1
Acetyl-CoA: 59.5
fatty_acid: 41.9
3-Methylbutyryl-CoA: 40.0
inactive: 31.4

clz7: mal

PKS_AT (567..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.6
inactive: 131.5
Methoxymalonyl-CoA: 78.1
Methylmalonyl-CoA: 73.3
Propionyl-CoA: 56.6
2-Methylbutyryl-CoA: 48.4
Isobutyryl-CoA: 47.1
Ethylmalonyl-CoA: 44.8
CHC-CoA: 34.0
Benzoyl-CoA: 30.9
Acetyl-CoA: 29.8
trans-1,2-CPDA: 28.1
fatty_acid: 21.2
3-Methylbutyryl-CoA: 12.9

clz14: X

AMP-binding (8..393): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLFYIAWVCK
Nearest Stachelhaus code(s):: Pro DLFYLAWVCK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor