LOCUS       BGC0001173              9945 bp    DNA     linear   BCT 24-OCT-2011
DEFINITION  Bacillus pumilus ATCC 7061 BAT.Contig118, whole genome shotgun
            sequence.
ACCESSION   BGC0001173
VERSION     BGC0001173
KEYWORDS    .
SOURCE      Bacillus pumilus ATCC 7061
  ORGANISM  Bacillus pumilus ATCC 7061
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     Source DNA is available from Dr. Kasthuri Venkateswaran, California
            Institute of Technology, Jet Propulsion Laboratory, Biotechnology
            and Planetary Protection Group; M/S 89-2, 4800 Oak Grove Dr.,
            Pasadena, CA 91109
            The NIAID funded Bioinformatics Resource Center PathoSystems
            Resource Integration Center (PATRIC; http://patricbrc.vbi.vt.edu/)
            is responsible for annotation updates of this record.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 23:38:32
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..804)
                     /codon_start=1
                     /locus_tag="BAT_2465"
                     /note="identified by match to protein family HMM PF08241;
                     match to protein family HMM PF08242"
                     /product="methyltransferase domain family"
                     /protein_id="EDW22947.1"
                     /transl_table=11
                     /translation="MIQEKIKELEVKRALAQSWFSDPDKRKISSNYDNRETPFTRFLSA
                     ETFTSYYQLTFKKTPVSILDIGCGQGQMLEYISKQLPLADLTGIDSSEEAIHCANKLNI
                     KANFICTDIKNFSSHAKIYDVILIHLCFGLFENPIELLEQLLPYLSNESMIYIVDLNRD
                     SIESGLSSVQSKEEELYIYDQYHASLTLSEFEQLLTYITKPREDMMYKIGTSIIGGFSP
                     FSMEFLSLIGNGNLQQTLRQAPDQYSSSTQKVPVLLHAWLIKNSG"
     protocluster    1..9945
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3691:4672]"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3692..4672
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     protocluster    1..9945
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1508:3689]"
                     /cutoff="20000"
                     /detection_rule="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /neighbourhood="10000"
                     /product="LAP"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      1509..3689
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /neighbourhood="10000"
                     /product="LAP"
                     /protocluster_number="2"
     cand_cluster    1..9945
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="neighbouring"
                     /product="LAP"
                     /product="RRE-containing"
                     /protoclusters="2"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..9945
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001173"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9945
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="LAP"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="((((goadsporin_like or PF00881 or TIGR03605) and
                     (YcaO or TIGR03604)) or TIGR03882 or TIGR03603 or
                     TIGR03602) and not cyanobactin_synth)"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..804)
                     /locus_tag="BAT_2465"
     gene            complement(833..1504)
                     /locus_tag="BAT_2466"
     CDS             complement(833..1504)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="BAT_2466"
                     /note="identified by match to protein family HMM PF02517"
                     /product="caax amino protease family"
                     /protein_id="EDW23292.1"
                     /sec_met_domain="Abi (E-value: 1.1e-13, bitscore: 41.3,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIIAALGILIHFLGPLSSKMVVKIMRDKSTANRRLTTYITPQR
                     SYDGLTIIGASICCGALSQELFVEVSNSKNVFGILFFFLLGVLLPSVLWKIKNKVIVFK
                     GRYAVNVDSIVFLPLTALSEEVIWRFILPALLMNLWESIAISICVSSIGFILLHIPLGG
                     AKSTAYMSLFTLITVISYFAFGILAAIAFHISHNLFIHFFHPLKVKEFKTKPPVLSETD
                     W"
     gene            complement(1509..2318)
                     /locus_tag="BAT_2467"
     CDS             complement(1509..2318)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     PF00881"
                     /gene_kind="biosynthetic"
                     /locus_tag="BAT_2467"
                     /note="identified by match to protein family HMM TIGR03605"
                     /product="SagB-type dehydrogenase domain protein"
                     /protein_id="EDW23125.1"
                     /sec_met_domain="PF00881 (E-value: 6.3e-16, bitscore: 48.6,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSIERHATNLADVVYGNEALRFDDPSENWFEATKIYRGSMAWDA
                     PGVAGLLRSQTLQKIATHASKRFDDLPLISLPKPLNTNISLDDVIKGRRSADYFSGGSV
                     SLEQLSTVLQKSYGLVERKEGPRRPIPSGGALYPLDLYIVANKVDTLDKGVYHFDPYRR
                     GLTKMGDYSEKEFEKIMLQEESVQDFAFAIVISANFWRSRFKYGHRSYRFVFMEAGHVM
                     QNMIMLSTAQKINSRPFGGFIDDELMELICGFNGVDDAPIYTLIAGT"
     gene            complement(2343..3689)
                     /locus_tag="BAT_2468"
     CDS             complement(2343..3689)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03604"
                     /gene_kind="biosynthetic"
                     /locus_tag="BAT_2468"
                     /note="identified by match to protein family HMM PF02624;
                     match to protein family HMM TIGR00702"
                     /product="conserved hypothetical protein"
                     /protein_id="EDW22903.1"
                     /sec_met_domain="TIGR03604 (E-value: 1.8e-104, bitscore:
                     339.1, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 5.9e-69, bitscore: 222.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVNKWTTLEESWEILDQLADPYVGIVRREYRKLRDVDDIWGHSYG
                     AHGTDSNILFGVPSNSYNGGGSANPAAARLAAIGETVERYSAAYLPLNSDVVCFGTQKD
                     LINENKSVIGFDKWEMYAVTQYEDPNFPHNVWNEDTKLWWRKGHIAENNEEVWTPAQLI
                     HIANYTEWPGDPNIGHATSNGLACGITYQEAAISGLFETIERDAFMLTWYNKLSLPQID
                     INTSPRLKHFYERYIKPTSLDLHLVDMTVFSGVPTILAVIRNPHTDLAPFAIGAASAHS
                     IERACEKAAIEGMYTRTWVKTEQRLGNALVDVDYHRDINSFEDHIKLYAGTAIVKEADF
                     LTASKKLVDVNDYTPFDDGSPDILWTSLVSHLSKQGYHVATFDLTSPDIKDAGAYVVKS
                     IIPGYKPIDVLYRGRMLGGERILKHSYQLGLVDKPFKLEELNPIPHPFP"
     gene            complement(3692..4672)
                     /locus_tag="BAT_2469"
     CDS             complement(3692..4672)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Plantazolicin_C_RRE"
                     /gene_kind="biosynthetic"
                     /locus_tag="BAT_2469"
                     /product="conserved hypothetical protein"
                     /protein_id="EDW22765.1"
                     /sec_met_domain="Plantazolicin_C_RRE (E-value: 3.9e-22,
                     bitscore: 68.0, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVKEQESTRYKLAPHVTLGKLEDSLIGLKAQTVTKIEGEGIIEF
                     IQEISNHLTKDNGVSIKELEENFEIESDQLKHVLEFLVANGICVKTDMNFDLTALLSYE
                     RAGGQVLVEDILDRLKTGIVEVIAQDHNPVAVEFIRHLQEAGLHVVLKGEITKQPNIRV
                     AIGKSHLDPILEEINDLALEDHIPWLSIVPYDGQTSWVGPFFIPHQSACLHCYNLRKSA
                     NFSDEVLRSDLMKIKPVNPEKKPVYHMPINLVQIGIASNLILEWLTLKDYAPSATPGGF
                     TTINLDDKGVSVHNHRVYRVPRCPKCSPAADTGFPQVWFHGEVKN"
     gene            complement(4697..5395)
                     /locus_tag="BAT_2470"
     CDS             complement(4697..5395)
                     /codon_start=1
                     /locus_tag="BAT_2470"
                     /product="conserved hypothetical protein"
                     /protein_id="EDW22932.1"
                     /transl_table=11
                     /translation="MSIHYVGIREPIKVYLEKMKVDYDDYPVQVIDLGGAVNYDKEFFI
                     NNTDFQTETILLSNRRHNVEDELGSLESELLKIYQNSILYRTSPLDINIELYRGWAGAT
                     GKLITAFSPEGVNWINTIDVIKACLIPKKDVRSRAGKAYDLTGPELVSMSRLKDIFEKD
                     LDMKIELYCKSKEEVITILSQNNMPLDVLEWLVGFQEYSSSKQLQPFTKTLEQIIGHPP
                     TSAQLFKNTN"
     gene            5815..5940
                     /gene="pznA"
                     /locus_tag="BAT_4209"
     CDS             5815..5940
                     /codon_start=1
                     /gene="pznA"
                     /locus_tag="BAT_4209"
                     /product="plantazolicin"
                     /protein_id="EHD25717.1"
                     /transl_table=11
                     /translation="MTKITIPTALSAKVHGEGQHLFEPMAARCTCTTIISSSSTF"
     gene            complement(6278..6997)
                     /locus_tag="BAT_2471"
     CDS             complement(6278..6997)
                     /codon_start=1
                     /locus_tag="BAT_2471"
                     /product="conserved hypothetical protein"
                     /protein_id="EDW23486.1"
                     /transl_table=11
                     /translation="MLSEKLELYVESCFRPYKKDKTYEEKKKELLEMLGKVKLKLENAG
                     TKDEEVQDMLIEKLKSGEISIDVNTLDIDDHNRVEHVKKIFVNADLDWLTFINVKYKLC
                     DFKGLSIVQKVMNHSEFNGCYLRKMNVSNSKISTSTFEKSDLTQSHFEHCEITNSSLYG
                     CHLPNVRIDESTLINVSFENCYFKNISFENSKLINVNFKSCTFKKLNFIGVTMDKMTYY
                     ILEGEGADLSGVKVIDS"
     gene            complement(7142..7888)
                     /locus_tag="BAT_2472"
     CDS             complement(7142..7888)
                     /codon_start=1
                     /locus_tag="BAT_2472"
                     /note="identified by match to protein family HMM PF01061"
                     /product="putative ABC transporter permease"
                     /protein_id="EDW23538.1"
                     /transl_table=11
                     /translation="MWPLVRIESIKQVKDRSLLFWTILLPVASIILLIELFTSGSSQAQ
                     KTLVASQSVTGMSVFFSTFVIISIVISFVKDREKGIVARLASTPLQPHNFFLGKCVPFF
                     VIILFQVLLLTILGVYLYGIEITQYSSYFLVVVLLSMQVTSWGVAIAAFAKTENTGLVM
                     TNMIALGTAVACGLWTPFDQLPSYLKIIGMLFPQYWAHQGLVQSVSTLPGGENLILVTV
                     VLIGYTLLGFILSLVGYKKYIKRARG"
     gene            complement(8151..9044)
                     /locus_tag="BAT_2473"
     CDS             complement(8151..9044)
                     /EC_number="3.6.3.-"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 189.6; E-value:
                     1.2e-57)"
                     /gene_kind="transport"
                     /locus_tag="BAT_2473"
                     /note="identified by match to protein family HMM PF00005"
                     /product="Nod factor export ATP-binding protein I
                     (Nodulation ATP-binding protein I)"
                     /protein_id="EDW22956.1"
                     /transl_table=11
                     /translation="MLKIENVSKKYGASQALRSITLTIPEGVCYGLIGPNGSGKSTLMK
                     IISHIIKSYEGKVEHVGESKSALGYIPQDISLEEKLTAKVNMEFFGQIYGLKGKKLDER
                     VEKTLKDVGLWERANDIVEHFSGGMKRRLNIGCALMHAPELILLDEPTVGVDPQSRRYI
                     FDIINKLKEEGRTIIYVSHYMEEIESLCDYVAFIDKGKIVEEGKIGELLNRYAETSVFF
                     DSMIPNKTLTSLGYDFVTYQGGVLVKTTKPLETLEDLAKLSKKESLVPQQLSLYRPRLE
                     DVFFQLTGTDLRDQFQ"
     gene            complement(9066..9290)
                     /locus_tag="BAT_2474"
     CDS             complement(9066..9290)
                     /codon_start=1
                     /locus_tag="BAT_2474"
                     /product="regulatory protein, ArsR"
                     /protein_id="EDW22796.1"
                     /transl_table=11
                     /translation="MIYETCDFTFLKGSLSVTDGNLGSHLKKLEEAGYVLITKTFVDKK
                     PKTIVSITDLGEHAYKEYLSVIEAILKNK"
     gene            complement(9352..9945)
                     /locus_tag="BAT_2475"
     CDS             complement(9352..9945)
                     /codon_start=1
                     /locus_tag="BAT_2475"
                     /product="membrane protein, putative"
                     /protein_id="EDW23131.1"
                     /transl_table=11
                     /translation="MPNEKSNNSEILENLSHIKTITNYKTEYCKQTSIYFIIWGLIWIM
                     GYSIPLLFANPITIGVFWLVLGIVGWVITLTTYFKQRKMIPMPLFLRTQLQYTWLGLGI
                     ILGIFIFLICVGLLPYTVDNLPFYIVLLVSIMYLLLGIVLAKEIFIMGFWLSVLGVTTF
                     LLFPSLMNIIFAFIGGGSLLLTGVILKRKGQGNE"
ORIGIN
        1 ttagccagaa tttttaataa gccacgcatg taaaagtaca ggtactttct gcgttgaact
       61 gctatattga tctggagcct gtcttaacgt ttgttgtaaa tttccatttc ctattaacga
      121 taaaaattcc atactaaatg ggctaaatcc tccaattata gaagtaccaa tcttatacat
      181 catatcttct ctcggtttgg taatataggt gagtaactgt tcaaactcag ataatgtcaa
      241 cgaggcatga tattgatcat atatatacag ttcttcttct ttgctctgaa ctgaactcaa
      301 tccactttct atactatctc gatttaaatc aacaatataa atcatagatt catttgataa
      361 ataaggaagt aactgttcta ataattcgat cggattttca aatagcccaa aacacagatg
      421 tattaaaatc acatcataaa ttttagcatg tgatgaaaaa ttttttatat ctgtacaaat
      481 aaaattggct tttatattta atttatttgc acaatgtatg gcttcttcag atgaatcaat
      541 cccagtcaaa tcagctagag gtaattgctt agatatatac tcaagcatct gaccttgtcc
      601 acatcctata tctaaaatag agacaggtgt ttttttgaat gttagttgat aataagaagt
      661 gaaagtttca gctgatagaa atcgagtaaa tggtgtttct ctattatcat aatttgaact
      721 tatttttctt ttatctggat ccgaaaacca tgactgagcc agagctcttt ttacttcaag
      781 ctctttgatt ttttcttgta tcatcacagg gtttaccacc tttataaaag cctcaccagt
      841 cagtttcaga taatacggga ggtttcgttt tgaattcctt tactttgaga gggtgaaaaa
      901 aatgaatgaa caaattatga gaaatatgaa atgctatcgc tgctaaaatc ccaaatgcaa
      961 agtatgaaat aactgtaatt aatgtaaaaa gagacatata agcagttgac ttagcaccgc
     1021 ctaatggtat atgcagcaat ataaatccta tcgaacttac acagattgat attgcaatag
     1081 attcccataa attcattaat aaagccggaa gaataaagcg ccaaattact tcttctgata
     1141 gagcagtcaa tggaagaaaa acgattgaat caacattgac agcatatcta cctttaaata
     1201 caatcacttt gttttttatt ttccatagca ctgatggtaa aagtactcct aacaaaaaga
     1261 aaaataaaat accaaataca tttttactat tggagacttc aacaaagagt tcttgagaaa
     1321 gcgcaccaca gcatatagaa gctccgataa tggtgagtcc atcatagctc ctctgaggtg
     1381 tgatatatgt tgttagcctt cgattggctg ttgatttatc tctcatgatt ttgacaacca
     1441 ttttagaact taagggaccc aaaaagtgaa tgaggatccc taaagctgct atgattgaaa
     1501 tcatttattt aagtacccgc aatcaatgta taaattgggg catcatctac cccattaaat
     1561 ccacagatca attccatcaa ctcatcatcg ataaaccctc cgaacggtcg actattaatc
     1621 ttctgtgcag tagatagcat gatcatattt tgcatgacat gtcctgcctc cataaaaaca
     1681 aagcgataag aacgatgtcc atacttaaat cgagaacgcc aaaaattagc tgaaatgact
     1741 atagcaaatg caaaatcttg aactgattct tcttgtaaca ttattttctc aaattctttt
     1801 tcggagtaat cacccatttt agtcaatcct cttctatacg gatcgaaatg ataaacacct
     1861 ttgtctaaag tatccacttt attggctaca atatatagat ctagaggata gagcgcacca
     1921 ccagatggta taggacgtct aggtccttct ttacgctcta ccaatccata actcttctgt
     1981 aaaactgttg agagttgttc taatgaaaca ctccccccgc taaaataatc tgcacttctt
     2041 cgtcccttaa tgacatcgtc taaagatata tttgtattta atggtttagg tagtgaaatt
     2101 aatggtaggt catcaaatct tttacttgca tgtgtagcaa tcttttgcaa agtttgagat
     2161 cgaagcagtc ctgcaacacc aggtgcgtcc catgccattg aacctctata gatttttgtt
     2221 gcttcaaacc aattttcaga aggatcatca aaccgtaatg cttcgtttcc atacacaaca
     2281 tcagccaaat tagtagcatg tctttcaata ctattcatat atttaatcac ctttctacga
     2341 tattaaggaa atggatgcgg aattggattc agttcttcta atttaaaagg tttatctact
     2401 aaacctaatt gataagaatg ttttaaaatt cgttctccac ccaacattct acctctatat
     2461 agaacgtcaa tcggtttata tccaggaata attgatttta cgacatatgc accagcatct
     2521 tttatatcag gactggttaa atcaaaagtt gctacatgat atccttgttt tgataaatga
     2581 ctgacgagac tagtccataa gatatctgga gaaccatcat caaacggtgt ataatcatta
     2641 acatccacta atttcttact cgcagttaaa aaatcagctt ctttcacaat agctgttcca
     2701 gcatagagtt taatatgatc ttcaaaacta tttatatcac gatgataatc tacatctact
     2761 agcgcattcc ctaatcgttg ttctgttttt acccaagttc ttgtatacat tccctctatt
     2821 gcagctttct cacatgctct ttcaattgaa tgagcagagg cagcgccaat agcaaatgga
     2881 gctaaatctg tatgaggatt tcttatcact gccaaaatag taggtacacc agaaaaaacg
     2941 gtcatatcta ctaaatgcaa atcaagagat gtaggtttaa tatatcgttc atagaaatgt
     3001 tttaatctag gtgaagtatt aatatcgatc tgcggtaaac ttaacttatt gtaccaagta
     3061 agcataaatg catctctttc aattgtttca aataaacctg agattgccgc ttcttgatat
     3121 gtaataccac atgctagtcc attactagtt gcatgaccaa tatttgggtc tcctggccac
     3181 tcagtatagt ttgcaatatg aattaattga gcaggcgtcc aaacttcttc attattttca
     3241 gcaatatgac cttttctcca ccataatttt gtatcttcat tccatacatt atgaggaaaa
     3301 ttcggatctt catattgggt gacagcatac atttcccact tatcaaagcc aattactgat
     3361 ttgttttcat tgattaagtc cttttgtgtt ccaaaacaaa ctacatcaga gtttaaaggt
     3421 aaataagctg cactatatct ttctaccgtt tcacctatag ctgctaatct ggctgctgct
     3481 ggattagcag accctccccc attataagaa ttagatggta ctccaaataa aatattgctg
     3541 tctgtaccat gcgctccata tgaatgaccc catatatcat caacgtctct gagttttcta
     3601 tactctctac ggactatccc aacataagga tccgccaatt ggtcaagtat ctcccatgac
     3661 tcttctaatg ttgtccattt attaaccata attaattttt cacctcacca tgaaaccaaa
     3721 cttgaggaaa accggtatct gcagctggag aacacttagg gcatcttggt actctataaa
     3781 cacgatgatt atgaacagat actcccttat catctaaatt aattgttgta aatccacctg
     3841 gtgttgcact tggtgcgtaa tcttttaaag tgagccattc tagaattaaa ttacttgcaa
     3901 tcccgatttg aactaaatta atcggcatat gataaaccgg cttcttttca ggatttacag
     3961 gtttgatttt cattaagtct gaacgcagta cttcgtcaga aaagttcgct gattttcgaa
     4021 ggttataaca atgtaaacaa gctgattgat gtggtatgaa aaatggacct acccaagatg
     4081 tctgtccatc atatggcaca atgcttaacc aaggtatatg gtcttctaat gctaggtcat
     4141 ttatttcttc caaaatcgga tctaaatgag atttaccaat agcaaccctg atatttggtt
     4201 gcttagttat ttctccctta agcactacat gtagccctgc ctcttgcaaa tgacgaatga
     4261 attctacagc aactggattg tgatcctgcg caatcacttc aacaatccct gtttttaaac
     4321 gatctagtat atcttcaacc aatacttggc caccggctct ttcataagac aaaagcgcag
     4381 tcaaatcaaa gttcatatca gtcttaacac atattccatt cgcaactaaa aattcgagga
     4441 catgttttaa ttgatcactc tcaatttcaa aattctcctc tagctcttta attgaaaccc
     4501 cattatcttt cgtgagatga ttagaaattt cttgaataaa ttcaataatt ccttcacctt
     4561 caattttggt gacagtttgc gcttttaaac caatcaaact gtcttctaat ttacccagcg
     4621 taacgtgtgg tgctaattta tatctcgtgc tttcttgttc tttaacaaac atacattatt
     4681 cctcctttaa gaataactaa tttgtatttt taaataattg tgcacttgtt gggggatgac
     4741 caattatttg ttctagcgtt ttcgtaaatg gctgtaattg ttttgaagaa gaatattcct
     4801 gaaatccgac taaccattct aatacatcta aaggcatatt attttgggat aaaattgtta
     4861 taacctcttc tttactttta caatataatt caattttcat gtctaaatcc ttttcaaaga
     4921 tatccttaag cctactcata cttactaact ctggccctgt taaatcatat gcttttccag
     4981 cgcgacttct aacatctttt ttagggatca aacatgcctt tataacatca attgtattaa
     5041 tccaattaac tccctcggga gaaaatgcag ttattaattt tccagtagca cctgcccatc
     5101 ctctataaag ttctatattg atatcaagcg gtgaagtcct ataaagtata ctattctgat
     5161 aaattttcaa taattcactt tcaagagaac ctagttcatc ctcgacatta tgccttctat
     5221 tagataaaag aattgtttcc gtttgaaaat cagtattatt tataaaaaat tctttgtcat
     5281 aattgaccgc tccgcccaaa tcaataactt gaacagggta gtcgtcataa tcaactttca
     5341 tcttctctaa atagactttt attggttctc taataccgac ataatgaata ctcataaaca
     5401 gcacttccct cttttctctt tcttatctct cagttagata aaatgattaa acttttaaca
     5461 gaaatagtaa tcttcttttt gagttcttca taacatagtt aacctcgact tttttcatac
     5521 atttacttaa cttaatatct aaatacccaa aataattgat ccaaattaac atcacccctc
     5581 aattaaaaaa taacatctag accatataat gactttaaat acataaaatt ttaattatag
     5641 tattattttc catggtcata ttaaaccaca cttttttggt gttatcaatg tacttttcag
     5701 aaaatataaa actttcacac ttaattattg acaaataaag acttatgaac catcatatgt
     5761 aatatatcaa ctacataatc tatgttgata aattttattt aaggaggttt aaagatgact
     5821 aaaattacaa ttccaactgc tttgtctgct aaggtacatg gggaaggtca acatttattt
     5881 gaaccaatgg ctgcgcgctg cacttgcaca acaatcatct caagttcttc aactttctaa
     5941 tcaactttag gttttttcgt aatatatatg attgaaaaaa cctcccacac acttagcgaa
     6001 gcagattagt taggtgtgtg gtcatctttt atttggaaaa atagaaattt tccacactcc
     6061 ttaacgaaaa ccttgcattt aactcgcttt tgtaaagctc cctcttataa ttaaactaat
     6121 tcttgtactt caatttaaat tctcaacaca actatttctc ttacatatat ttgaatttca
     6181 acccaaagac acggtgatca tgatactaat ggggtaatct agagatagtg gataaaacaa
     6241 aaaaggctat tgagattttt ctcaacagcc tactacatta actatcgatt actttcacac
     6301 cgcttaaatc agccccttct ccttctaaga tataataggt cattttatcc attgtcactc
     6361 caataaaatt taatttttta aacgtacatg atttgaaatt aacatttatt aatttagaat
     6421 tctcaaaaga gatgttttta aaataacaat tttcaaatga aacatttatt aaagttgatt
     6481 catcaatacg aacattgggt aaatgacagc catataaaga tgaattcgtt atctcacaat
     6541 gttcaaaatg agattgagtc aaatcactct tttcaaaagt tgatgtggaa attttagaat
     6601 tgctaacatt catttttctc agataacacc cattaaattc tgaatggttc atcacttttt
     6661 gtacgatcga tagcccttta aaatcgcaca acttatactt aacatttata aaagtcaacc
     6721 aatctaaatc agcattaaca aatatttttt tcacatgctc tactcggtta tgatcatcaa
     6781 tatctaatgt attgacatca atgcttattt cacctgattt cagcttttca ataagcatat
     6841 cctgaacttc ttcatctttt gttccagcat tctccagttt taatttaact tttccaagca
     6901 tctctaataa ttcttttttc ttttcttcat atgttttgtc ttttttatat ggccgaaaac
     6961 aactttcaac atataattct aacttttcac ttaacattgc cccactcctt ttatataaaa
     7021 agacggctta caagtattga tttttcggtc aacgttctag aaataaacta attgaagttt
     7081 agataaatca ttaaaattta agttagttta tgtgcataac attcagctga aattttatag
     7141 cctagcctct ggctctcttt atatattttt tataaccaac caacgaaaga ataaatccta
     7201 acagtgtata acctataagg actactgtta ctaagatcaa attctccccc ccgggtagtg
     7261 ttgatactga ttgcactaag ccttgatgtg cccaatactg tgggaagagc attccaatta
     7321 tttttaaata actaggtaat tgatcaaatg gagtccataa accacaagca accgctgtcc
     7381 ctaaagcaat catgttcgtc atcactaaac ctgtattctc agttttagca aatgcggcga
     7441 tggctactcc ccatgaagtg acttgcattg ataataggac aactactaag aaatacgagc
     7501 tgtattgtgt gatttcaata ccatataagt aaacacccaa aatggttaac aacaaaactt
     7561 gaaataaaat gatgacaaag aacggaacac actttccaag aaaaaaatta tgcggctgta
     7621 agggagtact tgccagcctt gccacaattc ctttctctcg atcttttaca aagctaatta
     7681 caattgaaat aataacaaat gtggaaaaaa acacactcat tcctgtaacc gattgagaag
     7741 caactaatgt tttttgtgct tgtgaactac ctgatgtaaa taattctatt aaaagaataa
     7801 ttgaagcaac tggcaacaaa atggtccaga acaacaaaga tcgatctttc acttgtttta
     7861 tggattcgat gcggactaat ggccacataa aaatcactcc cgttatatta atttaattcg
     7921 ctttcttata tcacttttaa aataatcaca ttttttaatt attttaccat ttatagatgt
     7981 atttaattat aattagtcaa ataatcttaa ctaacctact tattatttta aattgaaggt
     8041 tcacacaagg taattctgtt cgtttgatgt atgcctctta actatgtcaa tcactcttat
     8101 aagttatatg aattaaaata cgagagtggt tttcattcat aaagacattt ttattggaat
     8161 tgatcacgta aatctgttcc tgttaattga aaaaatacat cttcgagtct aggtctgtac
     8221 aaagataatt gttggggaac caatgattct tttttactca gtttagccaa atcttctaaa
     8281 gtttctaagg gttttgttgt tttaactaac acaccgcctt gataagttac aaaatcatac
     8341 ccgagactag ttaatgtttt attaggaatc atagaatcaa aaaagacaga tgtttctgca
     8401 taacgattca aaagttcacc tatttttcct tcttcaacaa tttttccttt atcaatgaaa
     8461 gccacataat cacataaact ctctatttcc tccatataat gactaacata gataatagtt
     8521 cttccctctt ccttcaattt gttgattatg tcaaaaatat agcgtcttga ttgaggatct
     8581 acccccactg tcggttcatc taaaagaatt aactcagggg cgtgcatgag agcgcagcca
     8641 atattcaaac ggcgtttcat tcctcctgaa aaatgttcaa ctatatcatt tgctctttcc
     8701 cacaacccta catcttttag tgttttttct actctttcat ctaatttctt cccttttaaa
     8761 ccataaatct gtccaaaaaa ctccatattt actttagctg ttaatttttc ctccaagctg
     8821 atgtcttggg ggatgtagcc tagcgcactt ttagactcac caacatgctc aactttgcct
     8881 tcataagatt taattatatg agatataatt ttcattaagg ttgatttacc agagccattt
     8941 ggacctatta atccataaca aactccttct ggaatcgtta atgtaatact tcttaacgct
     9001 tgggaagctc cgtatttttt actgacgttt tctattttca acattcggca ccctttcctt
     9061 tcatctcatt tgttttttaa aatggcttca ataacactca aatattcttt gtaagcgtgc
     9121 tctcctaaat ctgttatact aactattgtt tttggttttt tatccacaaa ggtctttgta
     9181 attagcacat agccagcttc ttctaatttt ttcaaatgcg agcccaaatt cccgtctgtt
     9241 acagataaag aaccttttaa gaaggtaaaa tcacatgttt cataaatcat taataaactc
     9301 ataatcccta accggacttt tggatggatt aaatcattta aactattgac attactcatt
     9361 tccttgtcct tttcttttta aaatcacacc agttaataac aaacttcctc caccaataaa
     9421 agcaaaaata atgttcatta aactaggaaa taataaaaat gtagttacac ctaaaacact
     9481 taaccagaac cccataataa aaatctcttt agctagaaca atacctaaaa gtaaatacat
     9541 tatggatacc aataaaacaa tataaaaggg taaattatct actgtatatg gtaataaacc
     9601 aacacatatt aaaaaaataa agatacctaa aatgattcca aggccaagcc atgtgtattg
     9661 caattgagtt cttaaaaaca agggcattgg tatcattttc ctttgtttaa aatatgtagt
     9721 taaagtgata acccagccca caatacctaa aacaagccaa aagacaccta tagttattgg
     9781 attagcaaaa agtaaaggta ttgaataccc cattatccaa ataaggcccc aaatgataaa
     9841 ataaatactt gtttgtttac aatactctgt tttatagttt gtaatcgtct tgatatgaga
     9901 taaattctct aatatttctg aattattcga tttttcattc ggcat
//
