BGC0001178 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001178 (Actinoplanes sp. ATCC 53533)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T3PKS	1	80,908	High	UK-68,597	NRPS:Type I

BGC0001178 - Region 1 - NRPS,T3PKS

Download region GenBank file

Download region SVG

Location: 1 - 80,908 nt. (total: 80,908 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 80,909
Label		BGC0001178

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

AGS77306.1: dehydrogenase

smcogs: SMCOG1072: dehydrogenase

AGS77321.1: Cyclization

MITE: MITE0000038Cyclization (identity: 73.70%)
smcogs: SMCOG1007: cytochrome P450

AGS77322.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

AGS77323.1: Cyclization

MITE: MITE0000041Cyclization (identity: 74.90%)
smcogs: SMCOG1007: cytochrome P450

AGS77326.1: Cyclization

MITE: MITE0000027Cyclization (identity: 69.80%)
smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase glycosyltransferase halogenase sulfotransferase transferase

AGS77311.1: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

AGS77314.1: Glycosylation

MITE: MITE0000033Glycosylation (identity: 73.20%)
smcogs: SMCOG1102: glycosyltransferase

AGS77315.1: Glycosylation

MITE: MITE0000037Glycosylation (identity: 67.00%)
smcogs: SMCOG1102: glycosyltransferase

AGS77316.1: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

AGS77318.1: Glycosylation

MITE: MITE0000034Glycosylation (identity: 64.20%)
smcogs: SMCOG1102: glycosyltransferase

AGS77324.1: Sulfotransferase domain

extras: Sulfotransfer_1: Sulfotransferase domain

AGS77325.1: flavin-dependent tyrosine-like hpg halogenase

halogenases: flavin-dependent tyrosine-like hpg halogenase
MITE: MITE0000028Halogenation (identity: 83.50%)
smcogs: SMCOG1119: halogenase

AGS77327.1: ambiguous flavin-dependent halogenase

halogenases: ambiguous flavin-dependent halogenase
MITE: MITE0000040Halogenation (identity: 64.60%)
smcogs: SMCOG1119: halogenase

AGS77336.1: aminotransferase

smcogs: SMCOG1019: aminotransferase

Lyases: hydratase

AGS77342.1: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

AGS77343.1: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

AGS77307.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AGS77308.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AGS77309.1: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

AGS77310.1: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

AGS77340.1: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001178.5	1.04	NRPS	UK-68,597	Actinoplanes sp. ATCC 53533
BGC0002341.2	0.93	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.45	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0001132.4	0.38	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.38	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.37	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.37	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000322.3	0.37	NRPS	chloroeremomycin	Amycolatopsis orientalis
BGC0001825.4	0.37	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.36	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001178.5	0.99	NRPS	UK-68,597	Actinoplanes sp. ATCC 53533
BGC0002341.2	0.77	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000357.5	0.68	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 80908:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpg - D-Tyr) + (D-Dhpg) + (D-Hpg - D-Hpg - Tyr) + (Dhpg)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 56972:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpg - D-Tyr) + (D-Dhpg) + (D-Hpg - D-Hpg - Tyr) + (Dhpg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AGS77307.1: Hpg - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (15..402): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFHLGLLCK
Nearest Stachelhaus code(s):: Hpg DAFHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1037..1423): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGS77308.1: Dhpg

Search NORINE for peptide: strict or relaxed

AMP-binding (466..854): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: dhpg (Dhpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYNAGSLCK
Nearest Stachelhaus code(s):: Dhpg DAYNAGSLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGS77309.1: Hpg - Hpg - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (461..856): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DIFHLGLLCK
Nearest Stachelhaus code(s):: Hpg DIFHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1960..2352): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAVHLGLLCK
Nearest Stachelhaus code(s):: Hpg DAVHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3475..3861): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: bht (bOH-Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAGVCK
Nearest Stachelhaus code(s):: Tyr DASTIAGVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGS77310.1: Dhpg

Search NORINE for peptide: strict or relaxed

AMP-binding (472..870): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: dhpg (Dhpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DPYHGGTLCK
Nearest Stachelhaus code(s):: Dhpg DPYHGGTLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor