LOCUS       BGC0001184              7298 bp    DNA     linear   BCT 24-FEB-2014
DEFINITION  Bacillus amyloliquefaciens subsp. plantarum str. FZB42, complete
            genome.
ACCESSION   BGC0001184
VERSION     BGC0001184
KEYWORDS    .
SOURCE      Bacillus velezensis FZB42
  ORGANISM  Bacillus velezensis FZB42
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
COMMENT     [WARNING] On Jun 24, 2019 this sequence was replaced by CP000560.2.
            Source DNA is available from Rainer Borriss (Humboldt University
            Berlin, Institute of Biology, Bacterial Genetics Chausseestr. 117
            D-10115 Berlin, Germany).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:04:04
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7298
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1200)
                     /NRPS_PKS="Domain: Aminotran_1_2 (32-382). E-value:
                     1.5e-77. Score: 253.0. Matches aSDomain:
                     nrpspksdomains_RBAM_034880_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="ywfG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019:aminotransferase (Score: 292.2; E-value:
                     9.8e-89)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="RBAM_034880"
                     /note="putative aspartate aminotransferase (transaminase A)
                     YwfG"
                     /product="YwfG"
                     /protein_id="ABS75817.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.9e-78, bitscore:
                     253.0, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIIPSDVIKTLPKQEFSLVFQKVKELEKTGAQIINLGQGNPDLP
                     TPPHIVETLREAALNPSYHGYGPFRGYPFLKEAIAEFYEREYGVSVNPETEVALFGGGK
                     AGLYVLTQCLLNPGDIALVPNPGYPEYLSGITMARAELHEMPLHPENGYLPDFERIDPA
                     VLKKAKLMFLNYPNNPTGATADQAFYEKAAAFAKKHDIHLIHDFAYGAFEFDQNPASFL
                     QAKEAKTVGAELYSFSKTFNMAGWRMAFAVGNEKIIQAVNEFQDHVFVGMFGGLQQAAA
                     AALSGNPEHTERLKRTYKERIDFFTALCEKELGWSIEKPKGTFYVWAEIPNEFESSHQF
                     SDYLLEHAHVVVTPGEIFGSGGKRYVRISMVAKQGDLREFVLRIQKLGLSFPSLQETSR
                     "
     protocluster    1..7298
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2390:3809]"
                     /cutoff="20000"
                     /detection_rule="(LmbU or Neocarzinostat or bcpB or frbD or
                     mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)"
                     /neighbourhood="20000"
                     /product="other"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2391..3809
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(LmbU or Neocarzinostat or bcpB or frbD or
                     mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)"
                     /neighbourhood="20000"
                     /product="other"
                     /protocluster_number="1"
     cand_cluster    1..7298
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(LmbU or Neocarzinostat or bcpB or frbD
                     or mitE or vlmB or prnB or CaiA or bacilysin or
                     orf2_PTase)"
                     /kind="single"
                     /product="other"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7298
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001184"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7298
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="other"
                     /region_number="1"
                     /rules="(LmbU or Neocarzinostat or bcpB or frbD or mitE or
                     vlmB or prnB or CaiA or bacilysin or orf2_PTase)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..1200)
                     /gene="ywfG"
                     /locus_tag="RBAM_034880"
     aSDomain        complement(55..1104)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_RBAM_034880_Aminotran_1_2.1"
                     /evalue="1.50E-77"
                     /label="RBAM_034880_Aminotran_1_2.1"
                     /locus_tag="RBAM_034880"
                     /protein_end="382"
                     /protein_start="32"
                     /score="253.0"
                     /tool="antismash"
                     /translation="QIINLGQGNPDLPTPPHIVETLREAALNPSYHGYGPFRGYPFLKE
                     AIAEFYEREYGVSVNPETEVALFGGGKAGLYVLTQCLLNPGDIALVPNPGYPEYLSGIT
                     MARAELHEMPLHPENGYLPDFERIDPAVLKKAKLMFLNYPNNPTGATADQAFYEKAAAF
                     AKKHDIHLIHDFAYGAFEFDQNPASFLQAKEAKTVGAELYSFSKTFNMAGWRMAFAVGN
                     EKIIQAVNEFQDHVFVGMFGGLQQAAAAALSGNPEHTERLKRTYKERIDFFTALCEKEL
                     GWSIEKPKGTFYVWAEIPNEFESSHQFSDYLLEHAHVVVTPGEIFGSGGKRYVRISMVA
                     KQGDLREFVL"
     gene            complement(1213..2394)
                     /gene="bacE"
                     /locus_tag="RBAM_034890"
     CDS             complement(1213..2394)
                     /codon_start=1
                     /gene="bacE"
                     /gene_functions="transport (smcogs) SMCOG1020:major
                     facilitator transporter (Score: 141; E-value: 8.5e-43)"
                     /gene_kind="transport"
                     /locus_tag="RBAM_034890"
                     /note="anticapsin/bacilysin excretion protein"
                     /product="BacE"
                     /protein_id="ABS75818.1"
                     /transl_table=11
                     /translation="MKQLKPNSKYLLFGQALSFMGDYCVLPALLILSTYYHDYWVTSGV
                     IAVRSIPMVFQPFLGVLVDRLDRVKIMLWTDVIRGVIFLGLTFLPKGEYPLLFLALLFV
                     SYGSGVFFNPARLAVMSSLEADIKNINTLFAKATTISIIVGAAAGGLFLLGGSVELAVA
                     FNGVTYLVSAFFISRIKLQYVPIQSENVREAFQSFKEGLKEIKTNAFVLNAMFTMITMA
                     LLWGVVYSYFPIVSRFLGDGEIGNFILTFCIGFGGFIGAGLVSKWGFNNNKGLMYFTVL
                     SIVSLALFLFTPIFAVSVIAAILFFIAMEYGEVLAKVKVQENAANQIQGRIFSVAEASI
                     GLCIAVGSMLINIVDAAVIMAFIVLLVSGLFLHTKLVNKSFSERNNESEQIHL"
     gene            complement(2391..3809)
                     /gene="bacD"
                     /locus_tag="RBAM_034900"
                     /note="previous designation ywfE"
     CDS             complement(2391..3809)
                     /codon_start=1
                     /gene="bacD"
                     /gene_functions="biosynthetic (rule-based-clusters) other:
                     bacilysin"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063:argininosuccinate lyase/adenylosuccinate lyase
                     (Score: 65.4; E-value: 8.4e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="RBAM_034900"
                     /note="L-amino acid ligase; COG0439; bacilysin synthesis"
                     /product="BacD"
                     /protein_id="ABS75819.1"
                     /sec_met_domain="bacilysin (E-value: 0.0, bitscore: 1036.9,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHA
                     ALIEKYSVAVIKDKDYFKSLADFEHPDSIYWAHEDHDKPEEEVVEEIVKVAGMFAVDAI
                     TTNNELFIAPMAKACERLGLRGAGVQAAENARDKNKMRAAFNRAGVKSIKNRRVTTLED
                     FRAALQEIGTPLILKPTYLASSIGVTLIKERETAEAEFNRVNEYLKSINVPKAVTFEAP
                     FIAEEFLQGEYDDWYETSGYSDYISIEGIMADGEYFPVAIHDKTPQIGFTETSHITPSI
                     LDDDAKRKIVEAAKKANEGLGLENCATHTEIKLMKNREAGLIESAARFAGWNMIPNIKK
                     VFGVDMAQLLLDVLCFGKEADLPKGLLEQEPCYVADCHLYPQHFKENGQLPETAVDFVI
                     ESIDIPDGVLKGDTEIVSFSAAEAGTSVDLRLFEAFNSIAAFELKGSNSGDVAESIKQI
                     QQQAKLTAKYALPV"
     gene            complement(3827..4588)
                     /gene="bacC"
                     /locus_tag="RBAM_034910"
     CDS             complement(3827..4588)
                     /NRPS_PKS="Domain: PKS_KR (6-115). E-value: 3.3e-11. Score:
                     35.3. Matches aSDomain:
                     nrpspksdomains_RBAM_034910_PKS_KR.1"
                     /codon_start=1
                     /gene="bacC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     240.6; E-value: 2.9e-73)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="RBAM_034910"
                     /note="bacilysin synthesis; COG1028; bacilysin synthetase
                     C"
                     /product="BacC"
                     /protein_id="ABS75820.1"
                     /sec_met_domain="adh_short (E-value: 8.1e-38, bitscore:
                     119.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.4e-32, bitscore:
                     102.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLTDKTVLITGGASGIGYAAVQAFLNQQANVVVADIDEAQGEAM
                     IRKENNDRLHFVQTDITDEPACQNAIRSAADKFGGLDVLINNAGIEIVAPIHEMELSDW
                     NKVLNVNLTGMFLMSKHALKYMLKSGKGNIINTCSVGGVVAWPDIPAYNASKGGVLQLT
                     RSMAVDYAKHNIRVNCVCPGIIDTPLNEKSFLENNEGTLEEIKKEKAKVNPLLRLGKPE
                     EIANVMLFLASDLSSYMTGSAITADGGYTAQ"
     aSDomain        complement(4244..4570)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_RBAM_034910_PKS_KR.1"
                     /evalue="3.30E-11"
                     /label="RBAM_034910_PKS_KR.1"
                     /locus_tag="RBAM_034910"
                     /protein_end="115"
                     /protein_start="6"
                     /score="35.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TVLITGGASGIGYAAVQAFLNQQANVVVADIDEAQGEAMIRKENN
                     DRLHFVQTDITDEPACQNAIRSAADKFGGLDVLINNAGIEIVAPIHEMELSDWNKVLNV
                     NLTGM"
     CDS_motif       complement(4325..4378)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_RBAM_034910_0002"
                     /evalue="2.30E+01"
                     /label="PKSI-ER_m5"
                     /locus_tag="RBAM_034910"
                     /protein_end="88"
                     /protein_start="70"
                     /score="-1.8"
                     /tool="antismash"
                     /translation="IRSAADKFGGLDVLINNA"
     CDS_motif       complement(4520..4573)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_RBAM_034910_0001"
                     /evalue="3.60E-05"
                     /label="PKSI-ER_m5"
                     /locus_tag="RBAM_034910"
                     /protein_end="23"
                     /protein_start="5"
                     /score="16.4"
                     /tool="antismash"
                     /translation="KTVLITGGASGIGYAAVQ"
     gene            complement(4585..5298)
                     /gene="bacB"
                     /locus_tag="RBAM_034920"
     CDS             complement(4585..5298)
                     /codon_start=1
                     /gene="bacB"
                     /locus_tag="RBAM_034920"
                     /note="bacilysin biosynthesis; COG1917; bacilysin
                     synthetase B"
                     /product="BacB"
                     /protein_id="ABS75821.1"
                     /transl_table=11
                     /translation="MMKTEQDLQELYFPTPKLIEWDNGVRQYSSVRGDTEVLLSYVPPH
                     TNVEPHQHKEVQIGLVVSGELSMTVGDVTRKMTALESAYIAPPNVPHGARNETDQEVIA
                     IDIKRLKAGETYTSPEDYFLNIFKTRDLLPGMEVTFFVEDWVEIMLANIPGNGGEMPFH
                     KHRNEQIGICIGGGYDMTIEGCKVDMTFGTAYFCDPREDHGAINRFEKDSKSVNIFFPP
                     RYNRAKAKKLEAKES"
     gene            complement(5285..5908)
                     /gene="bacA"
                     /locus_tag="RBAM_034930"
     CDS             complement(5285..5908)
                     /codon_start=1
                     /gene="bacA"
                     /locus_tag="RBAM_034930"
                     /note="bacilysin biosynthesis; pfam00800; bacilysin
                     synthetase A"
                     /product="BacA"
                     /protein_id="ABS75822.1"
                     /transl_table=11
                     /translation="MVLMIILDNSIQTKSKAYSISKLITINTLGPEGTSSEYAAKNFIT
                     NFTLLQGVNSKLSLHDTFESCIEKTLQSPLEYTIVPHAYDGIKHFYMRPDLQLLQIFRC
                     DTPMYGLAVRPGFEYTDDMLDKTVIVSHPSPINLIKYFTRKDVTFDLVNSTSAAAKRVK
                     DGLSDIALTNELARQKYGLHFVKTFKSIPMSWSLFGKGEIHDEN"
     gene            complement(6060..7298)
                     /gene="ywfA"
                     /locus_tag="RBAM_034940"
     CDS             complement(6060..7298)
                     /codon_start=1
                     /gene="ywfA"
                     /gene_functions="transport (smcogs) SMCOG1184:major
                     facilitator transporter (Score: 232.2; E-value: 1.3e-70)"
                     /gene_kind="transport"
                     /locus_tag="RBAM_034940"
                     /note="conserved hypothetical protein YwfA"
                     /product="YwfA"
                     /protein_id="ABS75823.1"
                     /transl_table=11
                     /translation="MNSKQTDHIETKRRFPICLALALTLGVFAAGSEELVISPLLPDLA
                     QAFSSDVSVLALSISIYGLMIFIGAPLLVPLGDKYSRELSLLAGLLIFTAGTVICALAH
                     NLFFFFLGRALSGLAAGAFVPTAYAVVGDRVPYAYRGKVMGLIVSSWSLALIFGVPIGS
                     FIGGVLNWRWTFWIFAMMSVLVASLIFIEARRSTADGDKTEEENGRQAGTFRDALKVPR
                     VPVYLTITFCNMIGFYGMYSFLGTYLHRVLPGGNTASGLLIMVYGIGFSMSVFTGKIAD
                     KAGKMRSLIAALAVISIWLSCLAYAPSSMPFLVIGLFVWGLMQSLTVTLLSTILSDCSQ
                     SRRGKIMAFYSLASNLAVTLGSAVMGPVYVGYGYAAVGFICAAVTLIGFVLSVFAYRRY
                     GKYEHQADQTLYR"
ORIGIN
        1 tcagcgggat gtttcttgta atgaaggaaa ggacaaaccg agcttttgaa tccgcagcac
       61 gaattccctt aaatcgccct gcttggccac cattgatatt ctgacataac gctttccgcc
      121 gctgccgaaa atctcgccgg gcgtcacgac aacatgggca tgctccagca gatagtccga
      181 aaactggtgt gaagactcga attcattcgg aatttcagcc catacataaa aagtcccttt
      241 cggtttttca atgctccagc ctaattcctt ttcacataac gccgtgaaaa agtcaatccg
      301 ttctttatat gtgcgcttca gacgctccgt atgctcagga tttcctgaca gcgccgccgc
      361 tgccgcctgc tgaaggccgc cgaacatgcc gacgaagacg tgatcctgaa actcattgac
      421 cgcctgaatg attttctcat tccccacggc aaatgccatt ctccagcctg ccatattgaa
      481 ggttttcgaa aatgaataaa gctccgcgcc gaccgttttc gcttctttcg cctgaagaaa
      541 gctggccggg ttctgatcaa attcgaacgc gccgtatgca aaatcgtgaa taaggtggat
      601 gtcatgtttt tttgcaaacg ctgccgcttt ttcataaaac gcctgatctg cagtcgcgcc
      661 cgtcgggtta ttcgggtaat tcaaaaacat cagcttggct tttttcagga cggcgggatc
      721 aatccgctca aaatccggca aatagccgtt ttccggatga agcggcatct catgcagctc
      781 cgctctggcc attgtaatgc ccgataaata ctcagggtag ccggggttcg ggactaaggc
      841 gatatcgccg ggattcagca ggcattgcgt caaaacgtac aaaccagctt tccctccgcc
      901 gaacagcgcg acttctgtct ccggattgac cgataccccg tactctctct cataaaattc
      961 cgcgatggct tctttcagaa acggatagcc tctgaacggg ccgtatccgt gatacgaagg
     1021 attcagcgca gcctcccgca atgtttcaac gatatgcggc ggtgtgggga gatcgggatt
     1081 cccctgcccg agattaatga tctgggctcc cgttttctcc aactctttta ccttttgaaa
     1141 aacgagcgaa aattcctgtt tcggaagtgt cttgatgacg tcggacggta tgatttccat
     1201 ctgaaaggct ccttataaat gaatttgctc tgattcgtta ttccgttctg aaaaggattt
     1261 attgaccagt tttgtatgca gaaacagccc gctgaccaaa aggacgataa acgccatgat
     1321 gactgccgcg tctacaatgt tgatcagcat ggaaccgacg gcgatgcaca atccgatgga
     1381 cgcctccgcc accgaaaaga tccggccctg aatctggttc gccgcgttct cctgaacttt
     1441 gaccttcgcc agcacctcac cgtattccat tgcgataaag aaaagaatgg cggcaatgac
     1501 tgacacggcg aaaatcgggg taaacaaaaa cagcgcaagc gagacgatcg ataacactgt
     1561 aaaatacatc agccctttat tgttgttaaa gccccatttg ctgacaagac cggcgccgat
     1621 aaagccgccg aagccgatac agaacgtcag gatgaaattt ccgatttccc cgtcgcctaa
     1681 aaagcggctg acaatcggga aatagctgta gacgacaccc cacaaaagcg ccatcgtaat
     1741 catcgtgaac atggcgttta acacaaaagc atttgttttg atttccttca ggccttcttt
     1801 aaacgactga aaagcttctc tcacgttttc tgactgaatc ggcacgtatt gaagcttaat
     1861 gcggctgatg aaaaacgcgg agacaagata ggtcaccccg ttaaaggcga cggcaagctc
     1921 cacagaaccg cctaacagaa aaagtccgcc ggctgcagcg ccgacaataa tcgaaatcgt
     1981 cgtggctttt gcaaacaaag tgttgatatt tttaatatcc gcttctaaag acgacatgac
     2041 cgcgagacgt gccgggttaa aaaacacgcc gcttccgtaa gaaacaaaca gcagcgccag
     2101 aaaaagcaac ggatattcgc ctttcgggag aaatgtcagc cccaaaaaga tgacacctct
     2161 gatcacatcc gtccacagca tgattttcac cctgtcgagc cggtccacga gcacgcctaa
     2221 aaacggctga aacaccatcg gaatactgcg gaccgcgatg acgccggacg tcacccagta
     2281 gtcgtgatag tatgtagaca aaatgagcag agccgggaga acgcaatagt ctcccataaa
     2341 cgaaagcgcc tgcccgaaaa gcaaatactt agagttcggt ttcagctgtt tcataccggt
     2401 aacgcatact ttgcagtcag cttcgcctgc tgctgaattt gtttgattga ttcggccacg
     2461 tcacccgaat tgcttccttt cagctcgaac gccgcaatgc tgttgaacgc ttcgaacagc
     2521 cgcagatcca cggatgtacc cgcctcggcc gccgagaaag aaacgatttc ggtgtctccc
     2581 tttaagacgc cgtcgggaat gtcaatgctt tcaatgacga aatcgacagc cgtctcaggc
     2641 agctggccgt tctctttgaa atgctgagga tacaagtggc agtcggcgac atagcatggc
     2701 tcctgctcca ataatccttt cggcagatcg gcttcttttc cgaaacagag aacatccaat
     2761 aacagctgcg ccatatcgac gccgaagacc tttttaatat tcggaatcat gttccagccc
     2821 gcaaatcgtg ccgctgattc aatcagtccg gcttcccggt ttttcattaa tttaatctct
     2881 gtatgtgttg cgcagttttc gaggccgagt ccttcattcg cctttttggc tgcttcgacg
     2941 attttccgct tcgcgtcatc atccaggatg gacggcgtaa tatgcgatgt ctccgtgaat
     3001 ccgatttgcg gtgttttatc atgaattgcg acagggaagt attctccgtc ggccatgatg
     3061 ccttctatgc tgatatagtc ggaataaccg cttgtttcgt accagtcgtc atactcgccc
     3121 tgcaaaaatt cttccgcgat aaacggcgct tcaaacgtga ccgctttcgg tacgttaatc
     3181 gacttcaggt attcattgac tctgttaaat tcggcttctg ccgtttccct ctctttgatg
     3241 agcgtcacgc cgatggagct cgccagatat gtaggcttca gaatgagcgg cgttccgatt
     3301 tcctgaagcg cggcgcggaa atcttccaac gtcgtcaccc gtctgttttt gatagacttg
     3361 acgccggccc ggttgaaagc ggctctcatt ttgtttttat ctctggcatt ttcagcggcc
     3421 tgtacgcccg ctccccgcag gccgagacgt tcacacgctt ttgccatcgg agcgataaac
     3481 agttcattgt tggtcgtaat ggcgtcaacc gcgaacatgc cggccacctt gacgatttct
     3541 tcgactactt cttcctcagg tttgtcatga tcttcatgag cccagtaaat cgaatcggga
     3601 tgctcaaaat cagccagact cttaaaatag tctttatctt ttatgaccgc gaccgagtat
     3661 ttttcaatta atgccgcatg agaggctgta attgcaaaag gtctcggaat aaagctgacg
     3721 aggttgtatt tttcggctgc gcttttgtaa aacatatgcg gcgggcatcc cccaaggtca
     3781 gcgataacca atactgtttt tctctccata tgtaaacact ccttctctat tgtgcggtgt
     3841 atccaccgtc tgcggtgatc gcgcttccgg tcatatagct tgataaatcc gacgcgagaa
     3901 acagcatgac gttcgctatt tcttcaggct tgccgagtct cagcagcgga ttgactttcg
     3961 ctttttcttt tttgatttct tcaagcgtgc cttcgttgtt ttcaaggaac gacttttcat
     4021 tcaaaggtgt gtcaatgatt cccgggcaca cgcaattgac gcggatgttg tgtttggcgt
     4081 aatcgacggc catcgaacgc gtcagctgca atacgccgcc tttagaggcg ttatatgcgg
     4141 gaatgtcggg ccatgcaacg actcctccga cggagcacgt gttaatgata ttgcccttgc
     4201 cgcttttgag catatacttg agcgcgtgct tgctcataag aaacatgccc gtcagattga
     4261 cgttgagcac cttgttccaa tcgctcagct ccatctcatg aatcggggcg acaatttcga
     4321 tgccggcgtt attgattaac acgtccaatc cgccgaactt gtccgccgct gaccggatgg
     4381 cgttttgaca tgccggctca tctgtaatat ccgtctgcac aaaatgcagc ctgtcattgt
     4441 tttctttcct gatcatcgct tctccctgcg cctcatcaat atccgcgacg accacattcg
     4501 cctgctgatt cagaaacgcc tgaaccgcgg cataaccgat gcctgatgcg ccccctgtga
     4561 tgaggacggt tttatccgtc aggttcatga ctctttcgcc tctaattttt tcgcttttgc
     4621 tctgttatag cgcggaggga aaaagatatt gactgatttt gaatcttttt caaatctgtt
     4681 gatggcgccg tgatcttctc tcggatcgca gaaataagct gtgccgaatg tcatatccac
     4741 tttgcagcct tcaatggtca tatcatagcc gccgccgatg cagatgccga tctgttcatt
     4801 gcggtgttta tggaacggca tttctccccc gttgcccgga atgttggcaa gcatgatttc
     4861 cacccagtct tccacgaaga acgtgacttc cataccgggc aataagtctc tcgttttaaa
     4921 aatattcaaa aaataatctt cagggcttgt gtaggtttca ccggctttca ggcgtttaat
     4981 atcaatggcg atcacttctt gatccgtctc atttcttgcg ccgtgaggca cattaggagg
     5041 cgcgatatag gctgattcca gcgctgtcat ctttcttgtg acatctccga cagtcatgga
     5101 cagctccccg gaaacgacaa ggccgatttg gacttcctta tgctgatgcg gttccacgtt
     5161 tgtatgcggc ggaacgtaag aaagcagaac ttccgtatct cctctgacgg acgaatattg
     5221 tcttactccg ttatcccatt ctattaattt aggcgtcggg aaataaagtt cctgcaaatc
     5281 ttgttcagtt ttcatcatga atctctcctt ttccgaataa tgaccagctc atgggaatgc
     5341 ttttaaatgt tttgacaaag tgaaggccgt acttttgtct ggccagctca tttgtcagcg
     5401 cgatgtcgga caaaccgtcc ttcactcttt tcgcggctgc gctcgttgag ttcaccagat
     5461 caaacgtgac atctttacgg gtaaaatact taatcaaatt gataggagac gggtgggata
     5521 cgatgacagt cttatcaagc atgtcatccg tatattcaaa accgggccgg acagccaggc
     5581 cgtacatcgg ggtgtcgcac ctgaaaattt gcagaagctg cagatccggt ctcatgtaga
     5641 agtgcttaat gccgtcatac gcgtgaggca caattgtgta ttccagcgga ctttgaagcg
     5701 ttttttcaat gcacgattca aatgtatcat gcagtgataa tttgctgttc accccttgga
     5761 gaagggtaaa attggtgatg aagttttttg ctgcgtactc actacttgtc ccttcaggac
     5821 cgagcgtgtt gatcgtaatc agtttagata ttgaataagc cttgcttttt gtttgaatgc
     5881 tattatccaa tataatcatg agcaccaacc aatctgttaa attttaagta aatattatcc
     5941 atagatgttc aagctgtcaa tattatttga aaatattgat aattattcct atatattaga
     6001 aaacacccta tgacataggg tgttaaaatt aacatttata aaattataga aactttattt
     6061 taccgataca atgtttggtc ggcctgatgc tcatatttcc cataccttct gtatgcgaaa
     6121 acgctgagga caaagccgat gagggtgacg gccgcacaaa taaatccgac agctgcatat
     6181 ccgtatccga catagaccgg acccatgaca gcagaaccca aggtgacggc caaattggac
     6241 gccagactgt aaaatgccat gattttgccc cggcggctct gtgagcaatc acttaaaatg
     6301 gtgctgagca aggtcaccgt caggctttgc atcaatcccc agacgaataa cccgatgacc
     6361 aaaaacggca ttgatgacgg cgcataggcg agacaggaaa gccatatgct gatcacggcc
     6421 aatgccgcga tcaaagagcg cattttcccc gccttatctg cgatttttcc ggtgaacacg
     6481 ctcatggaaa aaccgattcc gtacaccatg atcagcagcc ctgaagctgt atttcccccc
     6541 ggcagcacgc ggtgaagata tgtgccgaga aaggaataca tgccgtaaaa accgatcatg
     6601 ttgcaaaaag tgatcgtgag gtatacaggg acacggggca ccttcagcgc atcccggaaa
     6661 gtgcctgcct gccgtccgtt ttcctcttct gttttatccc catccgccgt gctgcggcgc
     6721 gcctcaatga aaatgagcga agcaacgagg acagacatca tggcgaaaat ccaaaacgtc
     6781 caccgccaat tcaaaacacc gccgataaaa gagccgatcg gaacaccgaa aatcaaagcg
     6841 agcgaccagc ttgacacgat cagacccatc accttgccgc ggtacgcata aggcacacgg
     6901 tcgccgacca ccgcatatgc tgtcggcaca aacgcacccg ccgcgagccc ggaaagcgcc
     6961 ctgcccagaa agaagaaaaa gagattgtgc gccagcgcgc agatcaccgt gccggccgta
     7021 aatataagca gccccgccag caggctgagt tcccgggaat atttatctcc cagcggaacg
     7081 agaagcggcg cgccgataaa aatcatcaat ccgtaaatac tgatggaaag cgccagcaca
     7141 ctgacatccg agctgaaagc ctgcgctaaa tccggaagca gcggagaaat caccagctct
     7201 tcagaaccgg cggcaaatac gcctaatgtc aaagcaagcg cgagacagat cggaaatcgg
     7261 cgtttcgttt cgatgtgatc ggtttgtttg ctgttcat
//
