BGC0001194 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001194 (Embleya scabrispora)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	72,530	hitachimycin	PKS	100%

BGC0001194 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 72,530 nt. (total: 72,530 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 72,531
Label		BGC0001194

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001194	1.00	Polyketide	hitachimycin	Streptomyces scabrisporus
BGC0000220	0.40	Polyketide	enterocin	Streptomyces maritimus
BGC0002366	0.39	Polyketide	enterocin, neoenterocin A, neoenterocin B	Streptomyces sp. SCSIO 11863
BGC0001164	0.33	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001925	0.33	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0001647	0.33	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0000122	0.32	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001163	0.31	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000996	0.31	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0000866	0.30	Other	polyhydroxyalkanoate	Burkholderia sp. DSM 9242

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001194	1.00	Polyketide	hitachimycin	Streptomyces scabrisporus
BGC0000220	0.72	Polyketide	enterocin	Streptomyces maritimus
BGC0002366	0.71	Polyketide	enterocin, neoenterocin A, neoenterocin B	Streptomyces sp. SCSIO 11863
BGC0001164	0.67	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001925	0.66	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0001647	0.66	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0000122	0.65	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001163	0.65	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000996	0.65	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0000866	0.64	Other	polyhydroxyalkanoate	Burkholderia sp. DSM 9242

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 72530:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal - redmal) + (ohmal - ccmal) + (ohmal) + (ccmal - pk - ccmal) + (ccmal)
Non-colinear ordering:: hitP1 + hitP2 + hitP5 + hitP4 + hitP3

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

hitP1

PKS_AT (670..960): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.3
inactive: 120.5
Methoxymalonyl-CoA: 65.2
Methylmalonyl-CoA: 60.1
Propionyl-CoA: 46.1
2-Methylbutyryl-CoA: 22.9
trans-1,2-CPDA: 16.9
CHC-CoA: 15.6
Ethylmalonyl-CoA: 15.2
Isobutyryl-CoA: 12.7
Acetyl-CoA: 11.4
Benzoyl-CoA: 10.1
fatty_acid: 7.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (2392..2683): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.9
inactive: 122.4
Methoxymalonyl-CoA: 57.5
Methylmalonyl-CoA: 47.0
Propionyl-CoA: 31.9
Ethylmalonyl-CoA: 27.0
Acetyl-CoA: 21.1
Benzoyl-CoA: 19.9
Isobutyryl-CoA: 19.8
2-Methylbutyryl-CoA: 17.7
CHC-CoA: 14.8
fatty_acid: 11.7
trans-1,2-CPDA: 10.7
3-Methylbutyryl-CoA: 0.0

hitB

AMP-binding (26..435): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFFHLTGACK
Nearest Stachelhaus code(s):: dclohBfa or Hpg DIFHLGLLCK (41% 8Å match); Hpg DIFHLGLLCK (38% 8Å match)
Stachelhaus code match:: 60% (weak)

hitC

PKS_AT (80..283): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 10.2
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

hitE

AMP-binding (19..416): X

nrpys: bMe-Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DCFFFALITK
Nearest Stachelhaus code(s):: bMe-Asp DCFFFALITK (82% 8Å match); Ala DCFFFALITK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

hitP2

PKS_AT (557..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.0
inactive: 107.5
Methoxymalonyl-CoA: 66.2
Methylmalonyl-CoA: 62.8
Propionyl-CoA: 47.0
Ethylmalonyl-CoA: 35.6
Isobutyryl-CoA: 30.1
2-Methylbutyryl-CoA: 28.7
Benzoyl-CoA: 26.2
Acetyl-CoA: 26.1
CHC-CoA: 23.7
fatty_acid: 16.7
trans-1,2-CPDA: 16.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (2088..2375): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.3
inactive: 97.6
Methoxymalonyl-CoA: 51.8
Methylmalonyl-CoA: 45.2
Propionyl-CoA: 35.4
Benzoyl-CoA: 24.1
Ethylmalonyl-CoA: 23.2
Isobutyryl-CoA: 22.4
2-Methylbutyryl-CoA: 20.3
Acetyl-CoA: 19.2
fatty_acid: 16.2
CHC-CoA: 10.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

hitP3

PKS_AT (549..836): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.7
inactive: 103.7
Methoxymalonyl-CoA: 57.1
Methylmalonyl-CoA: 54.7
Propionyl-CoA: 41.4
Benzoyl-CoA: 26.3
Ethylmalonyl-CoA: 24.8
2-Methylbutyryl-CoA: 24.3
Isobutyryl-CoA: 22.3
Acetyl-CoA: 18.7
fatty_acid: 14.0
CHC-CoA: 12.5
trans-1,2-CPDA: 8.6
3-Methylbutyryl-CoA: 0.0

hitP4

PKS_AT (574..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.2
inactive: 104.6
Methoxymalonyl-CoA: 49.7
Methylmalonyl-CoA: 46.2
Propionyl-CoA: 34.1
Ethylmalonyl-CoA: 25.4
Isobutyryl-CoA: 24.1
2-Methylbutyryl-CoA: 22.9
Benzoyl-CoA: 22.4
fatty_acid: 22.3
Acetyl-CoA: 20.3
CHC-CoA: 15.2
trans-1,2-CPDA: 9.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (2254..2551): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 213.3
Methylmalonyl-CoA: 212.2
Methoxymalonyl-CoA: 170.9
2-Methylbutyryl-CoA: 142.9
trans-1,2-CPDA: 138.3
Propionyl-CoA: 109.5
CHC-CoA: 95.1
Malonyl-CoA: 94.7
Benzoyl-CoA: 80.7
Isobutyryl-CoA: 74.0
Acetyl-CoA: 52.4
3-Methylbutyryl-CoA: 41.7
fatty_acid: 39.4
inactive: 23.4

PKS_AT (3946..4233): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.6
inactive: 104.5
Methoxymalonyl-CoA: 50.9
Methylmalonyl-CoA: 46.4
Propionyl-CoA: 35.2
Isobutyryl-CoA: 26.6
Ethylmalonyl-CoA: 25.1
2-Methylbutyryl-CoA: 24.4
Benzoyl-CoA: 23.6
fatty_acid: 23.2
Acetyl-CoA: 19.9
CHC-CoA: 13.9
trans-1,2-CPDA: 9.3
3-Methylbutyryl-CoA: 0.0

hitP5

PKS_AT (556..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 192.3
inactive: 89.0
Methoxymalonyl-CoA: 64.8
Methylmalonyl-CoA: 52.3
Propionyl-CoA: 34.4
Isobutyryl-CoA: 31.1
Ethylmalonyl-CoA: 28.1
Benzoyl-CoA: 23.6
Acetyl-CoA: 20.2
trans-1,2-CPDA: 14.1
fatty_acid: 13.8
CHC-CoA: 9.6
2-Methylbutyryl-CoA: 0.3
3-Methylbutyryl-CoA: 0.0