BGC0001195 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001195 (Nocardiopsis sp. CMB-M0232)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	76,702	nocardiopsin A/nocardiopsin B/nocardiopsin C/nocardiopsin D	NRPS:Type I+PKS	100%

BGC0001195 - Region 1 - NRPS,T1PKS

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Location: 1 - 76,702 nt. (total: 76,702 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 76,703
Label		BGC0001195

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001195	1.53	NRP, Polyketide	nocardiopsin A, nocardiopsin B, nocardiopsin C, nocardiopsin D	Nocardiopsis sp. CMB-M0232
BGC0000844	0.70	Other	clavulanic acid	Streptomyces clavuligerus
BGC0001928	0.67	Other	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002084	0.64	RiPP	biarylitide YYH, biarylitide YFH	Planomonospora sp. ID82291
BGC0002099	0.64	RiPP	biarylite-YFH	Planomonospora sp. ID107089
BGC0002631	0.63	RiPP	myxarylin	Pyxidicoccus fallax
BGC0000457	0.63	NRP	vicibactin	Rhizobium etli CFN 42
BGC0002226	0.57	NRP	acu-dioxomorpholine B, acu-dioxomorpholine A	Aspergillus aculeatus ATCC 16872
BGC0002171	0.56	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.56	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001195	1.00	NRP, Polyketide	nocardiopsin A, nocardiopsin B, nocardiopsin C, nocardiopsin D	Nocardiopsis sp. CMB-M0232
BGC0000067	0.68	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000844	0.68	Other	clavulanic acid	Streptomyces clavuligerus
BGC0001928	0.67	Other	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000457	0.66	NRP	vicibactin	Rhizobium etli CFN 42
BGC0002084	0.66	RiPP	biarylitide YYH, biarylitide YFH	Planomonospora sp. ID82291
BGC0000041	0.65	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002099	0.65	RiPP	biarylite-YFH	Planomonospora sp. ID107089
BGC0002631	0.65	RiPP	myxarylin	Pyxidicoccus fallax
BGC0001504	0.65	Polyketide	amycomicin	Amycolatopsis sp. AA4

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 76702:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpr) + (Me-mal - ohmal - Me-ccmal - Me-redmal) + (ohmal - ohmal - redmal) + (Me-ccmal - Me-mal - redmal) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 36304:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpr) + (Me-mal - ohmal - Me-ccmal - Me-redmal) + (ohmal - ohmal - redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AJW65406.1

Search NORINE for peptide: strict or relaxed

AMP-binding (501..903): Hpr

nrpys: Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DIQFSAHVVK
Nearest Stachelhaus code(s):: Hpr DIQFSAHVVK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AJW65407.1

PKS_AT (550..844): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 194.6
Ethylmalonyl-CoA: 193.3
Methoxymalonyl-CoA: 149.5
2-Methylbutyryl-CoA: 128.0
Propionyl-CoA: 104.1
CHC-CoA: 101.0
trans-1,2-CPDA: 97.9
Malonyl-CoA: 95.7
Isobutyryl-CoA: 49.6
Benzoyl-CoA: 43.8
Acetyl-CoA: 34.8
inactive: 25.4
3-Methylbutyryl-CoA: 21.8
fatty_acid: 19.7

PKS_AT (1549..1829): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 179.7
inactive: 86.9
Methylmalonyl-CoA: 78.0
Methoxymalonyl-CoA: 75.3
Isobutyryl-CoA: 46.8
Ethylmalonyl-CoA: 44.6
Acetyl-CoA: 29.5
Propionyl-CoA: 27.4
Benzoyl-CoA: 25.5
2-Methylbutyryl-CoA: 22.2
CHC-CoA: 21.3
trans-1,2-CPDA: 17.9
fatty_acid: 15.0
3-Methylbutyryl-CoA: 4.5

PKS_AT (3063..3357): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.2
Ethylmalonyl-CoA: 193.1
Methoxymalonyl-CoA: 154.1
2-Methylbutyryl-CoA: 131.7
Propionyl-CoA: 106.4
trans-1,2-CPDA: 105.2
CHC-CoA: 103.6
Malonyl-CoA: 94.3
Isobutyryl-CoA: 50.0
Benzoyl-CoA: 43.8
3-Methylbutyryl-CoA: 39.1
Acetyl-CoA: 36.5
fatty_acid: 25.8
inactive: 24.0

PKS_AT (4811..5105): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.2
Ethylmalonyl-CoA: 188.1
Methoxymalonyl-CoA: 159.3
2-Methylbutyryl-CoA: 130.5
trans-1,2-CPDA: 109.8
Propionyl-CoA: 107.3
CHC-CoA: 104.7
Malonyl-CoA: 98.0
Benzoyl-CoA: 54.2
Acetyl-CoA: 52.1
Isobutyryl-CoA: 51.5
3-Methylbutyryl-CoA: 42.2
inactive: 27.4
fatty_acid: 23.7

AJW65408.1

PKS_AT (537..815): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.5
Methylmalonyl-CoA: 83.5
inactive: 80.4
Methoxymalonyl-CoA: 79.9
Isobutyryl-CoA: 50.9
Ethylmalonyl-CoA: 42.4
Propionyl-CoA: 33.9
Benzoyl-CoA: 26.4
trans-1,2-CPDA: 25.2
Acetyl-CoA: 25.2
CHC-CoA: 23.7
2-Methylbutyryl-CoA: 21.5
fatty_acid: 15.6
3-Methylbutyryl-CoA: 2.2

PKS_AT (2038..2318): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.0
Methylmalonyl-CoA: 82.5
Methoxymalonyl-CoA: 81.1
inactive: 77.0
Isobutyryl-CoA: 49.5
Ethylmalonyl-CoA: 49.1
Propionyl-CoA: 38.9
Acetyl-CoA: 33.5
Benzoyl-CoA: 29.4
trans-1,2-CPDA: 24.7
2-Methylbutyryl-CoA: 24.5
CHC-CoA: 22.5
fatty_acid: 18.7
3-Methylbutyryl-CoA: 5.7

PKS_AT (3542..3822): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.6
inactive: 78.3
Methylmalonyl-CoA: 72.6
Methoxymalonyl-CoA: 72.4
Ethylmalonyl-CoA: 39.8
Isobutyryl-CoA: 37.2
Propionyl-CoA: 33.0
Benzoyl-CoA: 29.9
Acetyl-CoA: 27.2
2-Methylbutyryl-CoA: 22.7
trans-1,2-CPDA: 20.1
CHC-CoA: 18.2
fatty_acid: 12.7
3-Methylbutyryl-CoA: 3.5

AJW65409.1

PKS_AT (569..863): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.4
Ethylmalonyl-CoA: 197.5
Methoxymalonyl-CoA: 164.2
2-Methylbutyryl-CoA: 126.2
trans-1,2-CPDA: 112.7
CHC-CoA: 108.0
Propionyl-CoA: 106.8
Malonyl-CoA: 103.9
Isobutyryl-CoA: 56.4
Benzoyl-CoA: 55.0
Acetyl-CoA: 51.9
3-Methylbutyryl-CoA: 43.5
inactive: 27.6
fatty_acid: 24.5

PKS_AT (2313..2605): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.6
Ethylmalonyl-CoA: 198.6
Methoxymalonyl-CoA: 166.7
2-Methylbutyryl-CoA: 145.5
trans-1,2-CPDA: 128.8
Propionyl-CoA: 122.0
CHC-CoA: 119.3
Malonyl-CoA: 104.1
Isobutyryl-CoA: 71.7
Acetyl-CoA: 53.1
Benzoyl-CoA: 48.8
fatty_acid: 47.5
3-Methylbutyryl-CoA: 37.6
inactive: 34.5

PKS_AT (3325..3606): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.0
inactive: 86.0
Methylmalonyl-CoA: 71.1
Methoxymalonyl-CoA: 65.7
Isobutyryl-CoA: 37.2
Propionyl-CoA: 31.4
Ethylmalonyl-CoA: 27.2
Benzoyl-CoA: 25.3
Acetyl-CoA: 24.5
CHC-CoA: 21.5
2-Methylbutyryl-CoA: 20.2
fatty_acid: 13.1
trans-1,2-CPDA: 11.5
3-Methylbutyryl-CoA: 0.9

AJW65410.1

PKS_AT (563..857): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.4
inactive: 90.4
Methoxymalonyl-CoA: 72.7
Methylmalonyl-CoA: 66.7
Isobutyryl-CoA: 38.6
Propionyl-CoA: 38.0
Ethylmalonyl-CoA: 35.7
Benzoyl-CoA: 22.1
fatty_acid: 20.3
Acetyl-CoA: 17.5
trans-1,2-CPDA: 12.4
2-Methylbutyryl-CoA: 11.8
CHC-CoA: 10.6
3-Methylbutyryl-CoA: 0.0