BGC0001195 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001195 (Nocardiopsis sp. CMB-M0232)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	87,771	High	nocardiopsin A/nocardiopsin B/nocardiopsin C/nocardiopsin D	NRPS:Type I+PKS

BGC0001195 - Region 1 - NRPS,T1PKS

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Location: 1 - 87,771 nt. (total: 87,771 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 87,772
Label		BGC0001195

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

AJW65399.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

AJW65400.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

AJW65404.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase glycosyltransferase

AJW65408.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

AJW65410.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

AJW65414.1: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Hydrolases: deaminase

AJW65405.1: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

Ligases: ligase synthase

AJW65406.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AJW65407.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AJW65409.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001195.3	1.52	NRPS, PKS	nocardiopsin A, nocardiopsin B, nocardiopsin C, nocardiopsin D	Nocardiopsis sp. CMB-M0232
BGC0000342.4	0.74	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.65	NRPS	AM-toxin	Alternaria alternata
BGC0001833.4	0.65	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001825.4	0.63	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001195.3	1.00	NRPS, PKS	nocardiopsin A, nocardiopsin B, nocardiopsin C, nocardiopsin D	Nocardiopsis sp. CMB-M0232
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000067.5	0.67	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002192.3	0.66	PKS	FR901512	fungal sp. No.14919
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 87771:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpr) + (Me-mal - ohmal - Me-ccmal - Me-redmal) + (ohmal - ohmal - redmal) + (Me-ccmal - Me-mal - redmal) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 39841:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpr) + (Me-mal - ohmal - Me-ccmal - Me-redmal) + (ohmal - ohmal - redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AJW65406.1: Hpr

Search NORINE for peptide: strict or relaxed

AMP-binding (501..903): Hpr

nrpys: Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DIQFSAHVVK
Nearest Stachelhaus code(s):: Hpr DIQFSAHVVK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AJW65407.1: mmal - mal - mmal - mmal

PKS_AT (550..844): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 194.6
Ethylmalonyl-CoA: 193.3
Methoxymalonyl-CoA: 149.5
2-Methylbutyryl-CoA: 128.0
Propionyl-CoA: 104.1
CHC-CoA: 101.0
trans-1,2-CPDA: 97.9
Malonyl-CoA: 95.7
Isobutyryl-CoA: 49.6
Benzoyl-CoA: 43.8
Acetyl-CoA: 34.8
inactive: 25.4
3-Methylbutyryl-CoA: 21.8
fatty_acid: 19.7

PKS_AT (1549..1829): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 179.7
inactive: 86.9
Methylmalonyl-CoA: 78.0
Methoxymalonyl-CoA: 75.3
Isobutyryl-CoA: 46.8
Ethylmalonyl-CoA: 44.6
Acetyl-CoA: 29.5
Propionyl-CoA: 27.4
Benzoyl-CoA: 25.5
2-Methylbutyryl-CoA: 22.2
CHC-CoA: 21.3
trans-1,2-CPDA: 17.9
fatty_acid: 15.0
3-Methylbutyryl-CoA: 4.5

PKS_AT (3063..3357): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.2
Ethylmalonyl-CoA: 193.1
Methoxymalonyl-CoA: 154.1
2-Methylbutyryl-CoA: 131.7
Propionyl-CoA: 106.4
trans-1,2-CPDA: 105.2
CHC-CoA: 103.6
Malonyl-CoA: 94.3
Isobutyryl-CoA: 50.0
Benzoyl-CoA: 43.8
3-Methylbutyryl-CoA: 39.1
Acetyl-CoA: 36.5
fatty_acid: 25.8
inactive: 24.0

PKS_AT (4811..5105): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.2
Ethylmalonyl-CoA: 188.1
Methoxymalonyl-CoA: 159.3
2-Methylbutyryl-CoA: 130.5
trans-1,2-CPDA: 109.8
Propionyl-CoA: 107.3
CHC-CoA: 104.7
Malonyl-CoA: 98.0
Benzoyl-CoA: 54.2
Acetyl-CoA: 52.1
Isobutyryl-CoA: 51.5
3-Methylbutyryl-CoA: 42.2
inactive: 27.4
fatty_acid: 23.7

AJW65408.1: mal - mal - mal

PKS_AT (537..815): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.5
Methylmalonyl-CoA: 83.5
inactive: 80.4
Methoxymalonyl-CoA: 79.9
Isobutyryl-CoA: 50.9
Ethylmalonyl-CoA: 42.4
Propionyl-CoA: 33.9
Benzoyl-CoA: 26.4
trans-1,2-CPDA: 25.2
Acetyl-CoA: 25.2
CHC-CoA: 23.7
2-Methylbutyryl-CoA: 21.5
fatty_acid: 15.6
3-Methylbutyryl-CoA: 2.2

PKS_AT (2038..2318): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.0
Methylmalonyl-CoA: 82.5
Methoxymalonyl-CoA: 81.1
inactive: 77.0
Isobutyryl-CoA: 49.5
Ethylmalonyl-CoA: 49.1
Propionyl-CoA: 38.9
Acetyl-CoA: 33.5
Benzoyl-CoA: 29.4
trans-1,2-CPDA: 24.7
2-Methylbutyryl-CoA: 24.5
CHC-CoA: 22.5
fatty_acid: 18.7
3-Methylbutyryl-CoA: 5.7

PKS_AT (3542..3822): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.6
inactive: 78.3
Methylmalonyl-CoA: 72.6
Methoxymalonyl-CoA: 72.4
Ethylmalonyl-CoA: 39.8
Isobutyryl-CoA: 37.2
Propionyl-CoA: 33.0
Benzoyl-CoA: 29.9
Acetyl-CoA: 27.2
2-Methylbutyryl-CoA: 22.7
trans-1,2-CPDA: 20.1
CHC-CoA: 18.2
fatty_acid: 12.7
3-Methylbutyryl-CoA: 3.5

AJW65409.1: mmal - mmal - mal

PKS_AT (569..863): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.4
Ethylmalonyl-CoA: 197.5
Methoxymalonyl-CoA: 164.2
2-Methylbutyryl-CoA: 126.2
trans-1,2-CPDA: 112.7
CHC-CoA: 108.0
Propionyl-CoA: 106.8
Malonyl-CoA: 103.9
Isobutyryl-CoA: 56.4
Benzoyl-CoA: 55.0
Acetyl-CoA: 51.9
3-Methylbutyryl-CoA: 43.5
inactive: 27.6
fatty_acid: 24.5

PKS_AT (2313..2605): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.6
Ethylmalonyl-CoA: 198.6
Methoxymalonyl-CoA: 166.7
2-Methylbutyryl-CoA: 145.5
trans-1,2-CPDA: 128.8
Propionyl-CoA: 122.0
CHC-CoA: 119.3
Malonyl-CoA: 104.1
Isobutyryl-CoA: 71.7
Acetyl-CoA: 53.1
Benzoyl-CoA: 48.8
fatty_acid: 47.5
3-Methylbutyryl-CoA: 37.6
inactive: 34.5

PKS_AT (3325..3606): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.0
inactive: 86.0
Methylmalonyl-CoA: 71.1
Methoxymalonyl-CoA: 65.7
Isobutyryl-CoA: 37.2
Propionyl-CoA: 31.4
Ethylmalonyl-CoA: 27.2
Benzoyl-CoA: 25.3
Acetyl-CoA: 24.5
CHC-CoA: 21.5
2-Methylbutyryl-CoA: 20.2
fatty_acid: 13.1
trans-1,2-CPDA: 11.5
3-Methylbutyryl-CoA: 0.9

AJW65410.1: mal

PKS_AT (563..857): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.4
inactive: 90.4
Methoxymalonyl-CoA: 72.7
Methylmalonyl-CoA: 66.7
Isobutyryl-CoA: 38.6
Propionyl-CoA: 38.0
Ethylmalonyl-CoA: 35.7
Benzoyl-CoA: 22.1
fatty_acid: 20.3
Acetyl-CoA: 17.5
trans-1,2-CPDA: 12.4
2-Methylbutyryl-CoA: 11.8
CHC-CoA: 10.6
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor