LOCUS       BGC0001197             12679 bp    DNA     linear   BCT 28-JAN-2014
DEFINITION  Frankia sp. EAN1pec, complete genome.
ACCESSION   BGC0001197
VERSION     BGC0001197
KEYWORDS    .
SOURCE      Frankia sp. EAN1pec
  ORGANISM  Frankia sp. EAN1pec
            Bacteria; Actinobacteria; Frankiales; Frankiaceae; Frankia;
            unclassified Frankia.
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 3634642
            Source DNA and bacteria available from Louis S. Tisa
            (lst@hypatia.unh.edu)
            Contacts: Louis S. Tisa (lst@hypatia.unh.edu)
            Paul Richardson (microbes@cuba.jgi-psf.org)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:06:10
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12679
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12679
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[8314:10950]"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_product_classes="pentangular polyphenol"
                     /t2pks_product_classes="tetracenomycin"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
                     /tool="antismash"
     proto_core      8315..10950
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_product_classes="pentangular polyphenol"
                     /t2pks_product_classes="tetracenomycin"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
     cand_cluster    1..12679
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="single"
                     /product="T2PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..12679
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001197"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12679
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2ks and t2clf)"
                     /subregion_numbers="1"
                     /tool="antismash"
     sig_peptide     1..81
                     /locus_tag="Franean1_2384"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probability 0.999) with cleavage site probability 0.760 at
                     residue 27"
     gene            1..3399
                     /locus_tag="Franean1_2384"
     CDS             1..3399
                     /codon_start=1
                     /db_xref="InterPro:IPR003594"
                     /db_xref="InterPro:IPR003660"
                     /db_xref="InterPro:IPR005467"
                     /db_xref="InterPro:IPR013587"
                     /locus_tag="Franean1_2384"
                     /note="PFAM: ATP-binding region ATPase domain protein;
                     histidine kinase HAMP region domain protein; Nitrate and
                     nitrite sensing domain protein; KEGG: fal:FRAAL4397
                     hypothetical protein; putative signal peptide"
                     /product="integral membrane sensor signal transduction
                     histidine kinase"
                     /protein_id="ABW11816.1"
                     /transl_table=11
                     /translation="MLRNWPIRSKLVVILIVPLAALAVLSAIQVRGDVDNVRAAGRIEA
                     LADFSIKGNNLVHVLQRERYATNSYVGSNFLIPMLGQQAVDARKPVDDALAIYLADEQA
                     LPASARDALGSTLSSIRTHLDQLGEHRKQIDAHQTEIASNGAFYNEAVNELLLLNARIA
                     AGSSDAKLVNGSTTLAGIAQAKEQASQQRGSVAQMLSMGNPTPQMLRALLAYAGAETAW
                     TEQFRSTATPAELELFTNTVGRSATGVNQMRDDVVNRFSGQQAPDVGLEAWRTAADSKI
                     AQMHDVEVQIANDLAATSRDIASTASRDALLGSIGVAIILVLSVVISLLVASPMIGQLR
                     RLRGGALDVANDRLPSVVERLHRGEPVDIDAESFPVPATSKDEIGQLAEAFATVHEVAV
                     RTAVEQAAMRKSIGDTFLNLARRSQALIHRQLKIIDALERKETDPDELEELFRLDHLAT
                     RMRRHAEDLIVLSGSKPARGWRRPVPIKDVVRGAVAEVEDYTRVKVLPITGGAVSGHAV
                     GDVIHMFAELIENATSFSPPHTPVQVAGHPVSNGFVVEIEDRGLGMNQEEIDALNNRLA
                     NPPPFDLSTSERLGLFVVSRLAERHDVQVRLRPSPYGGTMAIVLLPATLLRASDDKGED
                     SGPREFAAVGASSVGAQGSDLAGSLNGTSERVGAGASGPESNGHWAGNIPGDTLDAAAL
                     STAGAVNGAGPSTDETLIDDLPVFATARSSWFVAEKPRGRNRTDDEGDDDGPIPPRRPG
                     ELTGPTRRAELPPSQDGAGYPEDSGDAGWSDGRSDPSASLFGPPPGGGEFGGSLFSAPP
                     DTGQFSGFGPNGFGDGGGYGDAGFGDSGGYAGGFGGGATDVGANGMAGNGSGGFADSDY
                     HTAEFSRSGFGTGGGFGNDSGHGNDGGHGNDGGPGNDGGLGQETGDGFGSAGGFGSGDG
                     FGSADSQPGAGLPSRSRTGRASGDYARADYTPPGQQGPAGDGTGYREPRADRGQEFGRP
                     MRENGYGGAEQAYPGRQDGGIGDGAFRRSARSSHPGQSTGGGSAYPGANPPPAAPPFDT
                     AAAAAEESADDGLDELGLPKRRRRANLAPQLRRENADAGRSSLAAGPRSPEEIRSMMSS
                     FQANFGRGIEDGTVSNDGDDVRKVT"
     gene            3399..3818
                     /locus_tag="Franean1_2385"
     CDS             3399..3818
                     /codon_start=1
                     /db_xref="InterPro:IPR004942"
                     /locus_tag="Franean1_2385"
                     /note="PFAM: Roadblock/LC7 family protein; KEGG:
                     fal:FRAAL4396 hypothetical protein"
                     /product="Roadblock/LC7 family protein"
                     /protein_id="ABW11817.1"
                     /transl_table=11
                     /translation="MRPLGPSENMNWLLNNLVKKVPEVTYVIVLSSDGLLLATSRGMDR
                     ATADQLAAVASGFNSLARGAGRFFGGGNVRQTIVEMEAGFLFVTAVGDGSCLAILSSPG
                     ADIGLIAYEMALLVTRVGEFLTPELRAEMQSALPI"
     gene            3936..4307
                     /locus_tag="Franean1_2386"
     CDS             3936..4307
                     /codon_start=1
                     /db_xref="InterPro:IPR007995"
                     /locus_tag="Franean1_2386"
                     /note="PFAM: protein of unknown function DUF742; KEGG:
                     fal:FRAAL4395 hypothetical protein"
                     /product="protein of unknown function DUF742"
                     /protein_id="ABW11818.1"
                     /transl_table=11
                     /translation="MSGDEEWFDDEAGPVVRPYAVTGGRTRPSNRTLDLVALVSTSPQG
                     RAIATTLEPEQRAIAVLCQRLQSVVEISARLNLPVGVARVLVGDMLDAGLVSVHRPERT
                     SDRPSVAIIEKVLSGLQAL"
     gene            4288..4911
                     /locus_tag="Franean1_2387"
     CDS             4288..4911
                     /codon_start=1
                     /db_xref="InterPro:IPR004130"
                     /locus_tag="Franean1_2387"
                     /note="PFAM: protein of unknown function ATP binding; KEGG:
                     fal:FRAAL4394 conserved hypothetical protein; putative
                     ATP/GTP-binding domain"
                     /product="protein of unknown function ATP binding"
                     /protein_id="ABW11819.1"
                     /transl_table=11
                     /translation="MDSRLSRTRAADTPAPHPVKIIVAGGFGVGKTTFVGAVSEIPPLT
                     TEAAMTSASIGVDDTSAVASKTTTTVAMDFGRITLVDSVILYLFGTPGQDRFWFMWDEL
                     VLGAIGAIVLVDTRRLADSFPAIDYFEERDIPFLIALNHFEGARRYRSEDIRAALDLDP
                     RRPMVLCDARQRESVRQSLIALVRHALEVVSAESQRPGLPTGSR"
     gene            4974..5972
                     /locus_tag="Franean1_2388"
     CDS             4974..5972
                     /codon_start=1
                     /db_xref="InterPro:IPR000792"
                     /locus_tag="Franean1_2388"
                     /note="PFAM: regulatory protein LuxR; KEGG: fal:FRAAL4393
                     putative LuxR-family transcriptional regulator"
                     /product="transcriptional regulator, LuxR family"
                     /protein_id="ABW11820.1"
                     /transl_table=11
                     /translation="MLELLGLDDVTLSAYRLWLRHPDMDVDGVATAVGQANDTVRRSRD
                     RLVELSLLLPSTEHPGHLVAVHPEAGLEHLLQAQHEALIRRHERLVRARAQVSTFVSEY
                     LDNRPGGDGAEVEHIDSADQARAELLTLVGRAEKEVLALHTRRGRPPALTAEVLPVELR
                     ALQRGVAMRSVLPRTVSTDETGVGYVRTVASHGLEARLADDPRIDATTVDGRVGLVQFV
                     QHGTDPHALIVRSPALANLVTMLFEQVWDSAEPLAAEADGDLESPADDRPNDTERLLLR
                     LLSLGVKDEAAARHLGVSVRTVRRMVADLMTRLDARSRFQAGTVAARRGWL"
     misc_feature    5811..5813
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6305..6682
                     /locus_tag="Franean1_2389"
     CDS             6305..6682
                     /codon_start=1
                     /db_xref="InterPro:IPR006765"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1225:Polyketide synthesis cyclase (Score: 151.8;
                     E-value: 9.1e-47)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC
                     C4-C21/C2-C23|C2-C19 (Score: 153.8; E-value: 3e-48)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Franean1_2389"
                     /note="PFAM: Polyketide synthesis cyclase; KEGG:
                     fal:FRAAL4392 hypothetical protein"
                     /product="Polyketide synthesis cyclase"
                     /protein_id="ABW11821.1"
                     /transl_table=11
                     /translation="MPTESGSVPNQRTTSQRTLIVARMNPEDAGAVADVFAESDAGELP
                     HLVGVARRDLFRFHGLYFHLIESRADINGTLPPVREHPLFVDVNSKLEKYIAAYDPQTW
                     RGPRDAMADSFYTWVADQNGP"
     gene            7083..7340
                     /locus_tag="Franean1_2390"
     CDS             7083..7340
                     /NRPS_PKS="Domain: PP-binding (8-73). E-value: 3.7e-11.
                     Score: 35.3. Matches aSDomain:
                     nrpspksdomains_Franean1_2390_PP-binding.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR006163"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (t2pks) ACP
                     (Score: 45.9; E-value: 9.8e-15)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Franean1_2390"
                     /note="KEGG: fal:FRAAL4390 putative actinorhodin polyketide
                     synthase acyl carrier protein (ACP) (ActI ORF3)"
                     /product="putative actinorhodin polyketide synthase acyl
                     carrier protein (ACP) (ActI ORF3)"
                     /protein_id="ABW11822.1"
                     /sec_met_domain="PP-binding (E-value: 9.3e-12, bitscore:
                     35.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLQWTADELLDFLVEQAGLPPEDRPAELGVTFTDIGLDSLAYLQ
                     LQAEVQERFGVDLPNEAPEGYTLADILTTVNSALLQSEVA"
     aSDomain        7107..7301
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Franean1_2390_PP-binding.1"
                     /evalue="3.70E-11"
                     /label="Franean1_2390_PP-binding.1"
                     /locus_tag="Franean1_2390"
                     /protein_end="73"
                     /protein_start="8"
                     /score="35.3"
                     /tool="antismash"
                     /translation="ELLDFLVEQAGLPPEDRPAELGVTFTDIGLDSLAYLQLQAEVQER
                     FGVDLPNEAPEGYTLADILT"
     gene            7391..7885
                     /locus_tag="Franean1_2391"
     CDS             7391..7885
                     /NRPS_PKS="Domain: Polyketide_cyc (10-129). E-value:
                     9.3e-19. Score: 59.8. Matches aSDomain:
                     nrpspksdomains_Franean1_2391_Polyketide_cyc.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR005031"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 210; E-value: 8.7e-64)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C9-C14
                     (Score: 230.9; E-value: 1.1e-71)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Franean1_2391"
                     /note="PFAM: cyclase/dehydrase; KEGG: fal:FRAAL4389
                     putative polyketide cyclase"
                     /product="cyclase/dehydrase"
                     /protein_id="ABW11823.1"
                     /transl_table=11
                     /translation="MSGHTDNSIFIDADIDLVWSMTNDLESWPSLFTEYASVEILEKAE
                     NTFKFRLTMHPDENGTSWSWVSERTLDPVNHKVRAYRVETGPFEYMHIEWTYLPEGTGT
                     RMRWVQDFQMRPTAPVTTEQMTQRINTNTPREMASIRGKVEAAASAAAPAADQVQVPEQ
                     V"
     aSDomain        7421..7777
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Franean1_2391_Polyketide_cyc.1"
                     /domain_subtypes="Polyketide_cyc"
                     /evalue="9.30E-19"
                     /label="Franean1_2391_Polyketide_cyc.1"
                     /locus_tag="Franean1_2391"
                     /protein_end="129"
                     /protein_start="10"
                     /score="59.8"
                     /tool="antismash"
                     /translation="IDADIDLVWSMTNDLESWPSLFTEYASVEILEKAENTFKFRLTMH
                     PDENGTSWSWVSERTLDPVNHKVRAYRVETGPFEYMHIEWTYLPEGTGTRMRWVQDFQM
                     RPTAPVTTEQMTQRI"
     gene            7896..8318
                     /locus_tag="Franean1_2392"
     CDS             7896..8318
                     /codon_start=1
                     /db_xref="InterPro:IPR013096"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C5-C18
                     (Score: 198.5; E-value: 7.9e-62)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Franean1_2392"
                     /note="PFAM: Cupin 2 conserved barrel domain protein; KEGG:
                     fal:FRAAL4388 polyketide synthase"
                     /product="Cupin 2 conserved barrel domain protein"
                     /protein_id="ABW11824.1"
                     /transl_table=11
                     /translation="MSIATPAPLTVPKVLSIHDVPANRRRGGDIRTLLSPATVGAKSGF
                     LGTLTLQPGEVVTEHWHPYSEEFLYCVTGAVTARLDGEHTALPAEHGLLIPIGMRHRIV
                     NTGDDVAFLVFHLSPLAPRPDLGHVDTEPLPEQSQQ"
     gene            8315..9667
                     /locus_tag="Franean1_2393"
     CDS             8315..9667
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (26-421). E-value:
                     7.9e-54. Score: 175.0. Matches aSDomain:
                     nrpspksdomains_Franean1_2393_PKS_KS.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR014030"
                     /db_xref="InterPro:IPR014031"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 477; E-value:
                     8.8e-145)"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     716.7; E-value: 1.9e-218)"
                     /gene_kind="biosynthetic"
                     /locus_tag="Franean1_2393"
                     /note="PFAM: Beta-ketoacyl synthase; KEGG: fal:FRAAL4387
                     putative 3-oxoacyl-[acyl-carrier-protein] synthase II"
                     /product="Beta-ketoacyl synthase"
                     /protein_id="ABW11825.1"
                     /sec_met_domain="t2ks (E-value: 6.2e-220, bitscore: 720.2,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKPTQRSRPDDAHGPASRDGGPRRTAVTGVGVVAPGGSNRKEFW
                     TLLTDGRTATRRLSFFDPSPFRSQVAAECDFDPLAAGLTRQEIARNDRFVQFALAAAAE
                     AVADSGLDLGLGVGGTEREPGDGPVVGVSMGSAVGATTRLENEYVVVSDGGKHWEVDPR
                     YASSFLYHALVPSCLATEIACRFGAHGPSFVVSTGCTSGIDAVGYGHQLIVDGEADIVI
                     AGASDAPLSPISIACFDAIRATSARNDDPEHASRPFDASRDGFVMGEGSAVLILEELES
                     ARARGAHIYCEVGGYASRSNAFHMTGLRPDGVEMAEAIRIALDQARLDPSAVDYVNAHG
                     SGTKQNDRHETAAFKRSLGAHAHEVPVSSIKSMVGHSLGAIGSIELAACALAIEHNVVP
                     PTANWSTRDPECDLDYVPNTARDHTVDAALSVGSGFGGFQSAIVLTSADRA"
     aSDomain        8393..9577
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Franean1_2393_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="7.90E-54"
                     /label="Franean1_2393_PKS_KS.1"
                     /locus_tag="Franean1_2393"
                     /protein_end="421"
                     /protein_start="26"
                     /score="175.0"
                     /tool="antismash"
                     /translation="AVTGVGVVAPGGSNRKEFWTLLTDGRTATRRLSFFDPSPFRSQVA
                     AECDFDPLAAGLTRQEIARNDRFVQFALAAAAEAVADSGLDLGLGVGGTEREPGDGPVV
                     GVSMGSAVGATTRLENEYVVVSDGGKHWEVDPRYASSFLYHALVPSCLATEIACRFGAH
                     GPSFVVSTGCTSGIDAVGYGHQLIVDGEADIVIAGASDAPLSPISIACFDAIRATSARN
                     DDPEHASRPFDASRDGFVMGEGSAVLILEELESARARGAHIYCEVGGYASRSNAFHMTG
                     LRPDGVEMAEAIRIALDQARLDPSAVDYVNAHGSGTKQNDRHETAAFKRSLGAHAHEVP
                     VSSIKSMVGHSLGAIGSIELAACALAIEHNVVPPTANWSTRDPECDLDYVPNTAR"
     CDS_motif       8540..8575
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Franean1_2393_0001"
                     /evalue="6.30E+01"
                     /label="PKSI-KS_m4"
                     /locus_tag="Franean1_2393"
                     /protein_end="87"
                     /protein_start="75"
                     /score="-1.8"
                     /tool="antismash"
                     /translation="FDPLAAGLTRQE"
     CDS_motif       9086..9124
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Franean1_2393_0002"
                     /evalue="8.20E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="Franean1_2393"
                     /protein_end="270"
                     /protein_start="257"
                     /score="9.6"
                     /tool="antismash"
                     /translation="FDASRDGFVMGEG"
     gene            9664..10950
                     /locus_tag="Franean1_2394"
     CDS             9664..10950
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (96-424). E-value:
                     3.4e-32. Score: 103.7. Matches aSDomain:
                     nrpspksdomains_Franean1_2394_PKS_KS.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR014030"
                     /db_xref="InterPro:IPR014031"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 594.2; E-value:
                     1.7e-180)"
                     /gene_functions="biosynthetic-additional (t2pks) CLF
                     (Score: 547.5; E-value: 4.1e-167)"
                     /gene_kind="biosynthetic"
                     /locus_tag="Franean1_2394"
                     /note="PFAM: Beta-ketoacyl synthase; KEGG: fal:FRAAL4386
                     putative polyketide beta-ketoacyl synthase 2 (pks family)"
                     /product="Beta-ketoacyl synthase"
                     /protein_id="ABW11826.1"
                     /sec_met_domain="t2clf (E-value: 8.7e-157, bitscore: 511.7,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVLDTAPSGTAGPARPAAGAVRPVITGLGVIAPSGVGLDAYWAS
                     TLRGELRVAPITRFDASRYDTRIAGEVPDFAAEEFVDHRYLVQTDRWTWMGMAASKLAL
                     ADAAYDPAEHDPYETSVVFGSGSGGNDFGQRELSRLWTRGRTAVSVYQSIAWFYAATTG
                     QTSIRHGLKGPSGVLVSDGAGGVDSLAQARRTIRRGTPTVLAGGAECGLTPYALTCHLS
                     SGRVSSAASPADGYKPFDVRANGYLPGEGGATLLVEDPAAAARRGARVYGEIAGYAATH
                     DGAHHEQAPADSRWLAAAMRRALADAELTPADVDVVFADGAGSPDLDALEAEAIRAVFG
                     AGAVPVTAPQGLIGRLCAGGSALAAATALLSIRDGVIPAVGNLDEPDPAHGLDLVRSPR
                     EGRVRAALVNARGHGGFNSSLVLRAVDTD"
     aSDomain        9952..10935
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Franean1_2394_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="3.40E-32"
                     /label="Franean1_2394_PKS_KS.1"
                     /locus_tag="Franean1_2394"
                     /protein_end="424"
                     /protein_start="96"
                     /score="103.7"
                     /tool="antismash"
                     /translation="MAASKLALADAAYDPAEHDPYETSVVFGSGSGGNDFGQRELSRLW
                     TRGRTAVSVYQSIAWFYAATTGQTSIRHGLKGPSGVLVSDGAGGVDSLAQARRTIRRGT
                     PTVLAGGAECGLTPYALTCHLSSGRVSSAASPADGYKPFDVRANGYLPGEGGATLLVED
                     PAAAARRGARVYGEIAGYAATHDGAHHEQAPADSRWLAAAMRRALADAELTPADVDVVF
                     ADGAGSPDLDALEAEAIRAVFGAGAVPVTAPQGLIGRLCAGGSALAAATALLSIRDGVI
                     PAVGNLDEPDPAHGLDLVRSPREGRVRAALVNARGHGGFNSSLVLRA"
     CDS_motif       10360..10416
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Franean1_2394_0001"
                     /evalue="1.60E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="Franean1_2394"
                     /protein_end="251"
                     /protein_start="232"
                     /score="11.7"
                     /tool="antismash"
                     /translation="ADGYKPFDVRANGYLPGEG"
     gene            11053..11790
                     /locus_tag="Franean1_2395"
     CDS             11053..11790
                     /NRPS_PKS="Domain: PKS_KR (10-170). E-value: 5.7e-14.
                     Score: 44.3. Matches aSDomain:
                     nrpspksdomains_Franean1_2395_PKS_KR.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR001509"
                     /db_xref="InterPro:IPR002198"
                     /db_xref="InterPro:IPR002347"
                     /db_xref="InterPro:IPR002424"
                     /db_xref="InterPro:IPR013968"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     228.4; E-value: 1.5e-69)"
                     /gene_functions="biosynthetic-additional (t2pks) KR C19
                     (Score: 338.1; E-value: 7.1e-104)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Franean1_2395"
                     /note="PFAM: NAD-dependent epimerase/dehydratase;
                     short-chain dehydrogenase/reductase SDR; KR domain protein;
                     KEGG: fal:FRAAL4385 3-oxoacyl-[acyl-carrier-protein]
                     reductase"
                     /product="short-chain dehydrogenase/reductase SDR"
                     /protein_id="ABW11827.1"
                     /sec_met_domain="adh_short (E-value: 7.7e-40, bitscore:
                     126.6, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 4.2e-40, bitscore:
                     128.1, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFPDLAGKRALVTGGTRGIGRAVVLGLAQAGATVVVGHQRQTEDA
                     ESLRRELKDTGNDHLLVQADVADPAQITELVSATGTHLGGLDIVVNSVGTISHIPYADL
                     PLDAWTQVLTTNLTATHLVTQGALPLLGDSGTVVNIGSGAGLVGVPLRAHYTAAKTGLI
                     GLTRSLSKELGSKGIRVNLVSPGVIETDQAAGMPPAARAAYTNRIPLGRLGEANEVAAV
                     VLFLASDVSSYVNGATFTVDGGI"
     CDS_motif       11083..11157
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Franean1_2395_0001"
                     /evalue="3.00E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="Franean1_2395"
                     /protein_end="35"
                     /protein_start="10"
                     /score="16.7"
                     /tool="antismash"
                     /translation="LVTGGTRGIGRAVVLGLAQAGATVV"
     aSDomain        11083..11562
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Franean1_2395_PKS_KR.1"
                     /evalue="5.70E-14"
                     /label="Franean1_2395_PKS_KR.1"
                     /locus_tag="Franean1_2395"
                     /protein_end="170"
                     /protein_start="10"
                     /score="44.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="LVTGGTRGIGRAVVLGLAQAGATVVVGHQRQTEDAESLRRELKDT
                     GNDHLLVQADVADPAQITELVSATGTHLGGLDIVVNSVGTISHIPYADLPLDAWTQVLT
                     TNLTATHLVTQGALPLLGDSGTVVNIGSGAGLVGVPLRAHYTAAKTGLIGLTRSLS"
     gene            11790..12113
                     /locus_tag="Franean1_2396"
     CDS             11790..12113
                     /codon_start=1
                     /db_xref="InterPro:IPR007138"
                     /locus_tag="Franean1_2396"
                     /note="PFAM: Antibiotic biosynthesis monooxygenase; KEGG:
                     fal:FRAAL4384 hypothetical protein"
                     /product="Antibiotic biosynthesis monooxygenase"
                     /protein_id="ABW11828.1"
                     /transl_table=11
                     /translation="MGHPQPFRIMLTMHIHPGREQEFEQTWLRIGDGVTSHPANLGQWL
                     IRSEDEPSTYYIISDWVDEPKFREFELSPGHLEHRKILHPLRASGSMVTGTVLTHLVGA
                     AAE"
     gene            12230..12679
                     /locus_tag="Franean1_2397"
     CDS             12230..12679
                     /codon_start=1
                     /db_xref="InterPro:IPR007138"
                     /locus_tag="Franean1_2397"
                     /note="PFAM: Antibiotic biosynthesis monooxygenase; KEGG:
                     fal:FRAAL4383 putative monooxygenase"
                     /product="Antibiotic biosynthesis monooxygenase"
                     /protein_id="ABW11829.1"
                     /transl_table=11
                     /translation="MAESSTGESAVAGSPASGPVEVEAEAEVEAEVEAEAGDGGRVRVM
                     LWAVDPAEDQGAVERAYHEISSKLVGTPGLLGNRLLRSAAEPTKFAVVSEWASMAEFVT
                     WERGADHKPATSPLRPLQDPAMRATIFTEVAAYGPDGPFTEPDGF"
ORIGIN
        1 atgctccgga attggcccat ccgctcgaag ctagtggtga ttctcatcgt cccgctggcc
       61 gcgctggccg tgctgagcgc gatacaggtg cgcggggacg tcgacaacgt ccgggccgcg
      121 ggccgcatcg aggcgctcgc cgacttctcg atcaaaggta acaacctggt ccacgtgctt
      181 cagcgggagc gctatgcgac caactcttac gttggatcga acttcctcat cccgatgctg
      241 ggccagcagg ctgtggatgc ccgcaagccc gtggacgacg ccctcgccat ctacctggcc
      301 gacgagcagg cactgcccgc gtccgcgcgc gacgccctcg gctcgacact gagttccatc
      361 cgtacgcatc tcgaccagct cggcgagcac cgcaagcaga tcgacgcgca ccagaccgag
      421 atcgcgtcga acggggcctt ctacaacgag gccgtgaacg agctgctgct gctcaacgcg
      481 cggatcgcgg ccggtagctc ggacgccaag ctggtcaacg gctctaccac gctggccggt
      541 atcgcccagg ccaaggaaca agcctcccag cagcgtggat ccgtcgccca gatgctctcc
      601 atgggcaacc ccacgccgca gatgctgcgt gctctgctgg cctacgccgg tgccgagacc
      661 gcctggaccg agcagttccg ctccacggcg acgccggccg agctggagct gttcacgaac
      721 acggtcgggc gctctgccac cggcgtcaac cagatgcgcg acgacgtggt caacaggttc
      781 agcgggcagc aggctcccga cgtcggcctc gaggcctggc ggaccgccgc cgacagcaag
      841 atcgcgcaga tgcacgacgt cgaggtgcag atcgccaacg acctggcggc gacaagtcgc
      901 gacatcgcct cgacggcctc ccgggacgcc ctgctcggaa gcatcggcgt ggcgatcatt
      961 ctcgtgctct cggtcgtgat ctcactgctc gtcgcgagcc ccatgatcgg tcagctccgc
     1021 cggctgcggg gtggcgcgct cgacgtcgcc aacgaccggc tgcccagcgt cgtcgagcgg
     1081 ctgcaccggg gtgagcccgt cgacatcgac gcggagtcct tcccggtacc cgcgaccagc
     1141 aaggacgaga tcggccagct ggcggaggcg ttcgccacgg tgcacgaggt ggcggtgcgc
     1201 accgccgtcg agcaggccgc gatgcgcaag agcatcgggg acaccttcct caacctcgcg
     1261 cgccgcagcc aggccctgat ccaccgccag ctgaagatca ttgacgcgct ggagcgcaag
     1321 gagaccgacc cggacgagct cgaggagctg ttccggctcg accacctcgc gacccgtatg
     1381 cggcgccacg ccgaggacct catcgtcctc tccggctcca agcccgcccg tggctggcgc
     1441 cggccggttc cgatcaagga cgtcgtccgc ggtgccgtcg ccgaggtcga ggactacacc
     1501 cgggtcaagg tcctgcccat caccggtggc gccgtctccg gccacgccgt cggtgacgtg
     1561 atccacatgt tcgccgagct gatcgagaac gcgacctcct tctcgccccc gcacaccccc
     1621 gtgcaggtcg ccgggcaccc ggtctccaac ggcttcgtgg tcgagatcga ggaccgcggg
     1681 ctcgggatga accaggagga gatcgacgcg ctcaacaacc ggctggcgaa cccgccgccg
     1741 ttcgacctgt cgaccagcga gcggctgggc ctgttcgtgg tcagccggct ggcggagcgg
     1801 cacgacgtcc aggtacggct gcggccgtcg ccgtacggcg gcacgatggc gatcgtcctg
     1861 ctgcccgcga ccctgctgcg cgcctccgac gacaagggcg aggacagcgg cccgcgcgag
     1921 ttcgccgcgg tcggcgcgtc gagcgtcggc gcccagggct cggacctggc cggctcgctc
     1981 aacggcacca gcgagcgggt gggggccggc gcgtccggcc cggagagcaa cggccactgg
     2041 gcgggaaaca tccccgggga caccctggac gcggcggcgc tgtcgacggc cggagccgtc
     2101 aacggtgccg gccccagcac cgacgagacg ctgatcgacg acctcccggt cttcgccacg
     2161 gcgcggtcga gctggttcgt ggcggagaag ccccgtggca ggaaccggac agacgacgag
     2221 ggggacgacg acgggccgat cccgccgcgc cgccccggcg agctgaccgg tccgacgcgg
     2281 cgggccgagc tcccgccgtc ccaggacgga gccggctacc cggaggactc gggggacgcg
     2341 ggctggtccg acggacggtc cgacccgtcg gcctcactgt tcggcccgcc tccgggcggc
     2401 ggcgagttcg gcggctcgct gttcagcgct ccgcctgaca ccggtcagtt cagcggcttc
     2461 ggtccgaacg gcttcggtga cggcggcggc tacggtgacg cgggcttcgg tgacagcggc
     2521 ggctacgccg gcggcttcgg tggcggcgcg accgatgtcg gcgccaacgg catggccggc
     2581 aacggatcgg gtggcttcgc tgactcggat taccacaccg ccgagttcag ccggtccggt
     2641 ttcggcaccg gcggcggctt cgggaacgac agcggtcacg ggaacgacgg cggtcacggg
     2701 aacgacggcg gtcccgggaa cgacggcggc ctggggcagg agaccggcga cggcttcggc
     2761 tcggccggtg gtttcggttc gggtgacggc ttcggttcgg ctgactccca gcccggggcc
     2821 gggctgccgt cccgctcccg gaccgggcgg gcctcgggcg actacgcccg cgccgactac
     2881 acgccaccgg gccagcaggg cccggccggg gacgggaccg gctaccgcga gccccgcgcg
     2941 gaccgcggcc aggaattcgg ccgtcccatg cgggagaacg ggtacggcgg ggcggaacag
     3001 gcctaccccg gacgccagga cggcggcatc ggcgacggcg ccttccgccg ctcggcccgg
     3061 tcgtcgcacc cggggcagtc caccggtggg ggctccgcct accccggggc caacccgccg
     3121 ccggcggcgc cgccgttcga cacggcagcc gccgcggcgg aggagtccgc cgacgacggt
     3181 ctcgacgagc tcggcctgcc caagcggcgg cggcgcgcga acctggcgcc gcagctgcgc
     3241 cgggagaacg cggacgcggg ccgttcgtcg ctcgccgcgg gccctcgttc acccgaggag
     3301 atccgcagca tgatgtcgtc cttccaggcc aacttcggcc gtgggattga ggacggaacg
     3361 gtgtccaatg acggagatga tgtgaggaag gtaacctgat gcgtccgctg gggccgtcgg
     3421 agaacatgaa ctggttgctc aacaacctgg tcaagaaggt ccccgaggtc acctatgtga
     3481 tcgtgctttc ttcggacggt ctgctgcttg cgacctcgcg tggaatggac cgggccaccg
     3541 ctgaccagct cgccgcggtg gcgtccggat tcaacagcct cgcccggggc gccggccggt
     3601 tcttcggcgg cggtaacgtt cggcagacca tcgtcgagat ggaggcggga ttcctgttcg
     3661 tcaccgcggt cggtgacggc tcgtgcctgg ctattctcag cagcccgggc gcggatatcg
     3721 gtctcatcgc ctacgagatg gcgctgctgg tgacgagggt gggtgagttt ctgacgccgg
     3781 agcttcgcgc cgaaatgcag tcggcgctgc cgatttagga cctgtccgaa tgcacgcgcg
     3841 gtggcgcgtc accggtttcc ggcccgttcc ccacgggctg gaaccggtga cgcgtccacc
     3901 gcgggcaaat gacgatccca ggcaggagga ccgcggtgtc aggggatgag gaatggttcg
     3961 acgacgaggc cgggccggtg gtgaggccct acgccgtcac gggggggcgc acccgtccca
     4021 gtaaccggac cctggatctg gtggccctgg tgtccacctc tccgcagggg cgggccatcg
     4081 cgaccacgct ggaaccggag cagcgtgcca tagcggtgct ctgccagcgg ctccagtccg
     4141 tcgtcgagat ctccgcccga ctcaacctcc cagtcggcgt ggcacgtgtg ctcgtcggcg
     4201 acatgctcga cgccgggctg gtgagtgtgc accggcccga acgaacgtcg gaccgcccca
     4261 gcgtggcgat tatcgaaaag gtgctcagtg gactccaggc tctctaggac ccgagcggcc
     4321 gatacaccgg cgccgcatcc ggtaaagatc atcgttgccg gtggtttcgg agtcgggaag
     4381 acgacgttcg tcggcgccgt gagcgagatt cccccgctca ccaccgaggc cgcgatgacg
     4441 tccgcgagta tcggtgttga cgacaccagc gccgtcgcct ccaagacgac gacgacggtg
     4501 gccatggact tcggtcgaat caccctggtc gacagcgtga ttctctacct gttcgggacc
     4561 cccggccagg accgcttctg gttcatgtgg gacgaactcg tcctgggtgc gatcggcgcg
     4621 atcgtgctcg tcgacacccg acgtctcgcc gactcgtttc ccgccatcga ctacttcgag
     4681 gaacgtgaca tcccgttcct catcgccctg aaccacttcg agggcgcgcg ccgttaccgc
     4741 agcgaggaca tccgcgcggc gctggacctc gacccccgcc ggccgatggt gctgtgcgac
     4801 gcccgccagc gggagtcggt gcgccagtcg ctcatcgcgt tggtccgcca cgccctggag
     4861 gtggtctccg cggagagcca gcgaccggga ctgccgaccg gctcacggtg aacgacaccg
     4921 cgcgtgccgc cccagcgccc gcggaccatc cgcttcgacc tgggaagggg atcttgctgg
     4981 agcttcttgg tctcgacgac gtcacgctgt cggcctaccg gttgtggctg agacatcccg
     5041 acatggatgt cgacggggtg gccacggcgg tggggcaggc gaacgacaca gtccgccggt
     5101 cacgggaccg gctcgtcgag ctgtcactgc tgttgccctc caccgaacac cccggccacc
     5161 tggtcgccgt ccacccggag gccggtctgg agcatctgct ccaggctcag cacgaggcac
     5221 tcatccgccg ccacgagcgg cttgtccggg cacgggcgca ggtgagcacg ttcgtctcgg
     5281 agtacctgga caaccggccc ggtggggacg gcgcggaggt cgagcacatc gacagcgccg
     5341 accaggcccg ggccgaactg ctcacccttg tcggccgggc cgagaaggag gtgctcgcgc
     5401 tgcacacccg gcgcggccgc ccgccggcgt tgaccgcgga ggttctcccg gtcgagctgc
     5461 gggcgctgca gcgcggtgtg gcgatgcgca gcgtcctccc acgcacggtc agcaccgacg
     5521 agacgggtgt cggctacgtc cgcacggtcg cctcgcacgg cctggaggcc cggctcgccg
     5581 acgacccgcg catcgacgcg acgaccgtgg acggccgggt cgggctggtg cagttcgtgc
     5641 agcacggcac cgacccgcac gcgctgatcg tgcgctcgcc cgcgctggcg aacctggtca
     5701 ccatgctgtt cgagcaggtc tgggacagcg ccgagccgct ggccgcggag gccgacggcg
     5761 acctggagag ccctgcggac gaccggccga acgacaccga gcggctgctt ttaaggctgc
     5821 tcagcctggg tgtgaaggac gaggcagcgg cccgccatct cggggtgtcc gtccgtacgg
     5881 tgcgtcggat ggtcgccgac ctcatgaccc gcctggacgc ccgtagccgc tttcaggccg
     5941 ggacggtcgc ggcccggcgg ggttggttgt agccgcgttg tgatcagttg gtcggatcgt
     6001 tggcagtaga tggtcagggc aacttcctgc cacccggaat gccggacaga agttccgatg
     6061 ggccgcatga ttccgatgag caaccggaat tacgtataac tggcgcggac caccacagca
     6121 ggaaccggcc aggtggcttc gccgaccggc gaacgcgtga gaccggtcac ttgcgcgtgt
     6181 cggtgccgag tgcatccggt acggccggtg ccttcgggtc gatccggccc ttcggtagcg
     6241 gtcggccgtg ttcgaacacg cgcggcgcag cgtgttcatc gtgactggcg aaagaggaga
     6301 tcgagtgccg acggaatccg gctcggttcc gaaccaacgc acgaccagcc agcggacgct
     6361 gattgtcgcc cggatgaacc ccgaagacgc tggtgcggtc gccgacgtct tcgcggagtc
     6421 ggacgccggc gagcttccac atctcgtcgg cgtcgcccgg cgcgaccttt tccgtttcca
     6481 cgggctgtac ttccatctca tcgagagccg ggcggacatc aacggcacgc tgccgccggt
     6541 tcgggagcac ccgctgttcg tcgacgtgaa cagcaagctc gagaagtaca tcgcggcgta
     6601 cgacccgcag acctggcgcg gcccccggga cgcgatggcc gacagtttct acacctgggt
     6661 ggccgatcag aacggtccct gagcccgcgc ccggccgcgc cggcgcggga ccaggttccg
     6721 cgccggtccc ggggctccgc ctccgcgcgg ccggccgccg caggacgaac gggccgtccc
     6781 gaccttcgtc gcatgcgcag ccatccgccc cgcgcggctg gtggccccgc cgtgcccgca
     6841 ggccgccctg cccgagcccc ggacgccgct gtccgggccg ggtccgcccg cccgcggcca
     6901 gcggtccgcc ggccatccgc tcccagcccc ccgaatgcag gtcaccgtgg tttcgggtgc
     6961 cctgggttca ggtcgcccgg tctccgaagc gctgtctttc gagcttacag gctgcccgag
     7021 tttcagagat tgatgtcaca accgtatccc gcggacgcgg gaatggtgag aagggaacgc
     7081 agatgcccct gcagtggacc gccgacgagc ttctcgactt cctcgtggag caggccggcc
     7141 tgccgccgga ggaccgtccg gccgaactcg gcgtgacgtt caccgacatc ggtctggact
     7201 cgctcgccta cctgcagctc caggccgagg tacaggagcg gttcggcgtc gacctgccga
     7261 acgaggcgcc ggagggatac accctcgccg acatcctcac cacggtgaac agcgcgctcc
     7321 tgcagtccga ggtcgcctga gactcccagg ccgaaaccat ttccctccag tgagcgaacc
     7381 ggagaaacac atgagcgggc acaccgacaa ctcgatcttc atcgatgccg acatcgacct
     7441 cgtctggtcc atgacgaacg acctggagtc ctggccgtcg ctgttcaccg agtacgcctc
     7501 ggtcgagatc ctcgagaagg ccgagaacac cttcaagttc cggctgacga tgcacccgga
     7561 cgagaacggg acgtcctgga gctgggtgtc cgagcgcacc ctcgacccgg tgaaccacaa
     7621 ggtccgcgcc taccgggtgg agacggggcc cttcgagtac atgcacatcg agtggaccta
     7681 cctgcccgag ggcaccggca cccggatgcg ctgggtgcag gacttccaga tgcgcccgac
     7741 ggcaccggtc accaccgagc agatgaccca gcggatcaac acgaacacgc cgcgcgagat
     7801 ggcgtccatc cgcggcaagg tcgaggcggc ggccagcgcc gccgcgccgg cagccgacca
     7861 ggtccaggtt cccgagcagg tctgaggaga taacggtgag catcgcaacc cccgcacccc
     7921 tgacggtccc caaggtcctc tcgatccacg acgtgccggc caaccggcgc cgcggcggtg
     7981 acatccgcac gctgctgtcc ccggcgaccg tcggcgccaa gtccggcttc ctgggaactc
     8041 tcaccctgca gcccggcgag gtggtgaccg agcactggca cccctactcc gaggagttcc
     8101 tctactgcgt caccggcgcg gtcacggccc gcctcgacgg cgagcacacc gccctgccgg
     8161 ccgagcacgg cctgctcatc ccgatcggca tgcggcaccg gatcgtgaac accggcgacg
     8221 acgtggcctt cctcgtcttc cacctcagcc cgctcgcgcc gcggcccgac ctggggcacg
     8281 tggacaccga gccgctgcct gagcagtcgc agcagtgagc aaacccacgc agcggtcgcg
     8341 gccggacgac gctcacgggc cggcgtcacg ggacgggggt ccgcgccgca ccgcggtgac
     8401 cggggtcggc gtcgtcgccc ccggtggctc gaaccgcaaa gagttctgga ccctgctcac
     8461 agacggccgc accgccaccc gccggctgtc cttcttcgac ccgtcgccgt tccggtcgca
     8521 ggtcgcggcc gagtgcgact tcgacccgct cgccgccggc ctcacccgcc aggagatcgc
     8581 gcgcaacgac cggttcgtgc agttcgcgct ggccgcggcc gcggaggcgg tggccgacag
     8641 cgggttggac ctcggcctcg gtgtcggcgg caccgaacgc gagccgggcg acgggccggt
     8701 cgtcggggtg agcatgggca gcgccgtcgg ggcgaccacc cggctcgaga acgagtacgt
     8761 ggtcgtcagc gacggcggga agcactggga ggtcgacccg cgctacgcga gctcgttcct
     8821 ctaccacgcg ctcgtgccga gctgcctggc caccgagatc gcctgccgct tcggcgcgca
     8881 cggcccgtcg ttcgtggtct ccaccgggtg cacctccggc atcgacgcgg tcggctacgg
     8941 ccaccagctc atcgtcgacg gcgaggcgga catcgtgatc gccggcgcct ccgacgcgcc
     9001 gctctcgccg atctccatcg cctgcttcga cgccatccgc gccacctcgg cccgcaacga
     9061 cgaccccgag cacgcctcgc ggccgttcga cgccagccgc gacggcttcg tgatggggga
     9121 gggcagcgcc gtgctgatcc tcgaggagct ggagtcggcc cgggcccgcg gcgcgcacat
     9181 ctactgcgag gtcggcgggt acgcctcgcg gtccaacgct ttccacatga ccggcctgcg
     9241 cccggacggc gtcgagatgg ccgaggccat ccgcatcgcc ctggaccagg cgcggctcga
     9301 cccgtccgcc gtcgactacg tcaacgccca cgggtcgggg acgaagcaga acgaccggca
     9361 cgagacggcc gctttcaaac ggtcgctggg cgcccacgcc cacgaggtgc cggtcagctc
     9421 gatcaagtcg atggtcggcc actccctcgg cgcgatcggc tccatcgagc tcgcggcctg
     9481 cgcgctggcg atcgagcaca acgtggtgcc gccgacggcg aactggtcga cccgcgaccc
     9541 cgagtgcgac ctggactacg tcccgaacac cgcccgcgac cacacggtgg acgcggcgct
     9601 gtcggtcggc agcgggttcg gcgggttcca gtcggccatc gtcctgacct cggcggaccg
     9661 ggcatgaccg tcctcgacac cgcgccgtcc gggacggccg gcccggcccg gccggccgcg
     9721 ggcgcggtgc ggccggtcat caccgggctc ggggtcatcg cgcccagcgg ggtgggcctc
     9781 gacgcgtact gggcctcgac gctgcgcggc gagctgcgcg tcgcgccgat cacccgcttc
     9841 gacgcgagcc gctacgacac ccggatcgcc ggcgaggtcc ccgacttcgc cgccgaggag
     9901 ttcgtcgacc accgctacct cgtgcagacc gaccgctgga cctggatggg catggccgcg
     9961 tccaagctgg cgctggccga cgcagcctac gacccggccg agcacgaccc gtacgagacg
    10021 tcggtggtct tcggcagcgg ctcggggggc aacgacttcg gccagcggga gctgtcccgg
    10081 ctgtggacgc gggggcgcac cgcggtcagc gtctaccagt cgatcgcctg gttctacgcg
    10141 gcgacgaccg gccagacctc gatccggcac gggctcaagg gcccgtcggg cgtcctcgtc
    10201 tccgacggcg ccggcggcgt cgacagcctc gcccaggccc ggcggaccat ccggcgcggc
    10261 acccccacgg tgctcgccgg cggcgccgag tgcgggctga cgccgtacgc gctgacctgc
    10321 cacctcagca gcggccgggt cagctccgcg gcgagccccg cggacggcta caagccgttc
    10381 gacgtccgcg ccaacggtta cctgcccggt gagggcgggg cgaccctgct cgtcgaggac
    10441 ccggctgccg cggcccgccg cggcgcccgc gtctacggcg agatcgccgg ctacgcggcc
    10501 acccacgacg gcgcgcacca cgagcaggcg ccggcggact cgcgctggct ggccgcggcg
    10561 atgcgccgcg cgctcgccga cgcggagctc acccccgccg acgtcgacgt cgtgttcgcc
    10621 gacggcgccg gctccccgga cctcgacgcg ctggaggcgg aggccatccg ggccgtcttc
    10681 ggcgccggag cggtgcccgt caccgccccg caggggctca tcggacggct gtgcgccggc
    10741 ggctccgccc tcgccgcggc gaccgcgctg ctgtcgatcc gcgacggggt gatccccgcc
    10801 gtgggcaacc tcgacgagcc cgaccccgcc cacggcctcg acctggtgcg ctcgccgcgt
    10861 gagggccgtg tccgggccgc gctggtcaac gcgcgcggcc acggcgggtt caacagctcg
    10921 ctcgtcctgc gggcggtcga caccgactga cctgcctctc cggcgcgccg acgacgccgt
    10981 cggcgcgccg ggggatcccg ggtggccaag gccaccggaa cgttccagac tcacctttcg
    11041 agaggatcac tgatgttccc agacctggcc ggcaagcgtg ccctggtcac cggcggcacg
    11101 cgcggtatcg gccgggccgt cgtgctcggg ctcgcccagg ccggcgccac cgtcgtggtc
    11161 ggccaccagc gccagaccga ggacgccgag agcctgcgcc gcgagctcaa ggacaccggc
    11221 aacgatcacc tgctggtgca ggccgacgtc gccgacccgg cgcagatcac cgagctggtg
    11281 tcggcgaccg gcacccacct cggcggcctc gacatcgtcg tgaacagcgt cgggacgatc
    11341 agccacatcc cgtacgccga cctgccgctg gacgcctgga cgcaggtgct gaccaccaac
    11401 ctgaccgcga cccacctcgt cacccagggt gcgctgccgc tgctcggcga ctccgggacg
    11461 gtcgtcaaca tcgggtccgg cgccgggctc gtcggcgtgc cgctgcgcgc ccactacacg
    11521 gcggccaaga cgggcctgat cggcctcacc cgctcgctgt ccaaggagct cggctccaag
    11581 ggcatccggg tgaacctggt ctcgccgggc gtcatcgaga ccgaccaggc ggccggcatg
    11641 cccccggcgg cgcgcgccgc gtacaccaac cgcatcccgc tgggccggct cggcgaggcc
    11701 aacgaggtcg ccgccgtcgt cctgttcctc gccagcgacg tgtccagcta cgtcaacggc
    11761 gccaccttca cggtcgacgg aggaatctga tgggccaccc ccagccgttc cgcatcatgc
    11821 tgaccatgca catccacccg ggccgcgagc aggagttcga gcagacctgg ctgcggatcg
    11881 gggacggcgt gaccagccac ccggcgaacc tcggccagtg gctgatccgc tccgaggacg
    11941 agccgagcac gtactacatc atcagcgact gggtggacga gccgaagttc cgcgagttcg
    12001 agctgagccc cgggcacctg gagcaccgca agatcctcca ccccctgcgg gccagcggct
    12061 cgatggtcac cgggaccgtg ctcacccacc ttgtcggcgc ggcggccgag tgatccgccg
    12121 caagaagaag gcggatgccc cgccggagac cgacccgcag gagaccgcct cagcggagtc
    12181 ggctccagcg gagtcggccg ctgcggagtc ggcgaccgcg gagccggctg tggccgagtc
    12241 gtccactggc gagtcggctg ttgccgggtc gcccgcgtcc ggcccggtcg aggtcgaggc
    12301 cgaggccgag gtcgaggccg aggtcgaggc tgaggccggt gacggtggcc gggtgcgggt
    12361 gatgctgtgg gcggtcgacc cggccgagga tcagggcgcg gtcgagcggg cctaccacga
    12421 gatcagctcc aagctggtgg gcacgcccgg cctgctcggg aaccgcctgc tgcgctccgc
    12481 cgccgagccg acgaagttcg ccgtcgtcag cgagtgggcg agcatggccg agttcgtgac
    12541 ctgggaacgc ggggcggacc acaagccggc cacctcgccg ctgcgtccgc tgcaggaccc
    12601 ggcgatgcgc gcgaccatct tcaccgaggt cgccgcctac ggcccggacg gccccttcac
    12661 cgagccggac ggattctga
//
