LOCUS       BGC0001229              8631 bp    DNA     linear   BCT 31-JAN-2014
DEFINITION  Streptomyces bingchenggensis BCW-1, complete genome.
ACCESSION   BGC0001229
VERSION     BGC0001229
KEYWORDS    .
SOURCE      Streptomyces bingchenggensis BCW-1
  ORGANISM  Streptomyces bingchenggensis BCW-1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     Source DNA is available from Yijun Yan, quasar110@126.com.
            Bacteria available from China General Microbiology Culture
            Collection Center (Accession No:CGMCC1734), Institute of
            Microbiology, Chinese Academy of Sciences.
            Genome was manually curated based on annotation generated by the
            NCBI Prokaryotic Genomes Automatic Annotation Pipeline Group.
            Information about the Pipeline can be found here:
            http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:10:31
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8631
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..8631
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[0:8080]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..8080
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..8631
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8631
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001229"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8631
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..5037)
                     /locus_tag="SBI_06987"
     CDS             complement(1..5037)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 256.5; E-value:
                     9.1e-78)"
                     /gene_kind="biosynthetic"
                     /locus_tag="SBI_06987"
                     /note="COG2274 ABC-type bacteriocin/lantibiotic exporters,
                     contain an N-terminal double-glycine peptidase domain"
                     /product="cytolysin B transport protein"
                     /protein_id="ADI10107.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.2e-31, bitscore:
                     96.1, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="DUF4135 (E-value: 2.8e-62, bitscore:
                     199.1, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 3.7e-24, bitscore:
                     73.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTWNTIVRRSGPDRRVPTITQVAQTECGLCCCVAVLRYHGRAEEI
                     SSARQVMEAGRDGLSATQLARFLRERGMDAKLFRVKDVSALAKFTSPVILYWEDHHFVV
                     LERFDGRRAVIMDPALGRRRLSRKELEAGFSGIAVSAEPGPDFEPTPRKFMAEWRGVSL
                     FAKGSRRRIAMVGFLSLGGYAGTLGIPMLTEWSVNRYARWGGLDDLATVLSVIIAVAVG
                     YFALYLLRVAVLSSVVAMMGEHLMIQTFSKLLSLPYKFFTTRQPGELLFRLNSVNVIRD
                     LLSSRVAQGVLDVGTLVCVSGYLLIAEWRLGLIAAGLSLVNALYLMKTRVRVLESVDTE
                     MTQLSKSQSAQLDAIVSVPTIKMGGYAAEFLAGWSKVYSSSLQAMKDRMRLQQGRISGL
                     TSTTQMFGPLILLLSSLYLVGHGQISLGQAMAVQTVSGTYFALSTSVFQTCTEFAEASR
                     YMARLNDIAETPSEDRGGDLKRLPSTSIRLTDIGFQYTRHSDPVLSDVSIDIPAGAKVA
                     LVGPSGSGKSTLGRIICGLYEPTSGTIEFGGRDMSEYDKDSLRRSIGYIPQEVHLHNRT
                     ILENLTMGQDIPEEQVREFCESVGILDFLQDLPMGLNTLISEMGANFSGGQRQRLAIVR
                     TLLQRPAILVLDEATASLDTINERRVTDIIQDIGATQIIIAHRLATIKAADRIYVLDRG
                     RVIEQGTHEELLNGGPVYMNLYAESDPDVLMSGPESTTGPEGSIPDPDVSIPAFLPFYQ
                     HLVPRSSVVAALREVMAPAARPERREAVLDAIWGELLTAVEGHSFRTLIGEFHTFREGL
                     GLPMTADVDEALQRFRRHLGAPGTCRRIADAFPVLRQRLTVLVDNSLDAYTDMFAAYMA
                     DQEALRADCLTPRTAAAGELIEDLFLTGSDPHNDNRQVIGVRLTSGTRLIFKPRALATD
                     SFVRDLYTAADPYLTFSLRDCLPMSVTSGSHGWQQFIPSEPMASPDQPARYFYRFGALC
                     AILSAIGASDLHDENLLAHGENPCVIDTETILRPDAGVGNDTLPHLLINHMKLSVVSTM
                     LVPSMSPASPVDVLMAGVGVAGDQPSKMTKTVIRDIGTDGMSVGRDPITYRQDANLPRL
                     GDVPLSATDYFSDVLSGYSDALEFVRGGAIPAILDAHRDMPVRYVLRATLVYARFMDAS
                     THPSYLARPEEAQRLFGLLGNSGQFTPELASHIGDEERAGMNTGNVPYFSSRADSTDLA
                     TSRVRFPEVFKTSSVDHARRGVTLNAARPDGYHHYLLEECFGEVFGEDSPAGLSRHSVF
                     AAPLRHARPGTWWPEIARRIADLGVTLHTPDGPRTGWLGGIGPGRAAGTITPGNFVSFH
                     DMGGIIAFLNRATTLDDGFRTIHHRADQGLDELLTEYGETLNQTPESVFSGASSLLLTR
                     PQAAPTDWLGSLLDKIDARSASNDLETDLSNGPAGLLMVLLSRLEAGDDPAALCGEARL
                     ARLRDLVLGHLQAPRTAPWFDLAHGELGLQWARARIGRVLGDETPARESADWLTARAGS
                     GTGTSLAGWCNGAAGLLLASAEILFSAGRADELSGRRLTTLVEAATRLPARRPVDLSVC
                     HGSSGVVQSLIATGRVLGDDSLLTRAREYQELVLTRARENGFYTGSPGRTSLLGYMLGW
                     AGIGDTDALLHAAEQNHDDGFFRVPVAMTSEFARPGQPVG"
     gene            complement(5039..8080)
                     /locus_tag="SBI_06988"
     CDS             complement(5039..8080)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     596.8; E-value: 1.3e-180)"
                     /gene_kind="biosynthetic"
                     /locus_tag="SBI_06988"
                     /note="COG4403 Lantibiotic modifying enzyme"
                     /product="hypothetical protein"
                     /protein_id="ADI10108.1"
                     /sec_met_domain="DUF4135 (E-value: 1.8e-71, bitscore:
                     229.3, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 7.6e-45, bitscore:
                     141.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTTAPYYPELSDAQISDSIAPLAQFDAQTLEFFRGIEIPRAAVS
                     RSAATRDAVTQWCAAPERYPFQKIIRNAAASLADPDPTAPFAPLFADPPDLLRQLLERA
                     EARLRAVYTRPLVVAVNHARRHGRLVGADPALRYRYFVDQSVRGSFEELSGLSFPVLRD
                     VTRVVLAYEAHNFRELCLRLAADRDAIAATFGIDAEDQVVSCGFADGDAHHHGRSVSVL
                     EFRSGRKLAYKPRDVSCEAAYAVIAREANDWLGTSLVAAKVLERAGYGYVEYVAAEDVS
                     DISARFMAASGELAAVMYLLNAQDMHLENVVATRRGPVPIDLETILQPVRLHLGTPEET
                     PDNAHHRIAQSVYGIGILPLLMAGRDEDSGYVDLGFLGEQGSGESPFKSLQFDAPFTDQ
                     VRLTLSKQAAEERQTVVGTLSQDEVHALGEGMAAGFVRVCRAVLSDKGRWTALVRKAAA
                     GVRVRYLHNPTMLYTQTLRTASSARAMSDPASYLAALKRVAIVSEPSARAIVRSELRQL
                     AGRDIPYFTVAATDVALTDGEGTEVEAALPTSPLDLAAAKVAALTEFEVGEQLRLIHSA
                     FSCRFPDNHLTSADDFDAPLPGATAGGRPTGTPGDTLAGVVTRLCDTMVASALPDRFAD
                     LPHTWIGPLASAQSNRPWPPAVLGYDLYTGRVGPALALAAAGRVLDHPAYRGLATQIFS
                     AIAEGLSDPLPDLPPETSDTRQTAPYSAYTGSAGMLFALAAAGRLLGQPDWVRAAQLAV
                     PDVLDLVAAGPAADRPLDMISGLAGVLSCVTAIGGPNAGESAATLTAMLTDALRPDAHH
                     PVLDQSGFGHGVSGVIHALSRAYPRLPEQQRKGVEATLSRLLDRLWNFYDPAEDDWHSN
                     MAAPGSFSTGWCHGSAGIALALTTYTAMTGDESVERMRKVAVGNLVHRGFGRNLTWCHG
                     DLGNHDILSTVASTEDSLHTAVADVERTWLRPEVFTRKAGDTRSRYAHTNSLLVGTAGM
                     VIHLVNRLDPSARLSPVELSTEGR"
     gene            complement(8209..8403)
                     /locus_tag="SBI_06989"
     CDS             complement(8209..8403)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SBI_06989"
                     /product="hypothetical protein"
                     /protein_id="ADI10109.1"
                     /transl_table=11
                     /translation="MQKDTDILGAWDEAELVELSEADVHGGTDPAVTGLILGIVAIQAG
                     VAVGVSAAFCPTTKCTSRC"
     CDS_motif       complement(8374..8403)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MQKDTDILGA"
                     /locus_tag="SBI_06989_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(8209..8373)
                     /prepeptide="core"
                     /locus_tag="SBI_06989_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-1.00"
                     /molecular_weight="5285.9"
                     /monoisotopic_mass="5282.7"
                     /core_sequence="WDEAELVELSEADVHGGTDPAVTGLILGIVAIQAGVAVGVSAA
                     FCPTTKCTSRC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MQKDTDILGA"
                     /leader_location="[8373:8403](-)"
                     /alternative_weights="5304.0"
                     /alternative_weights="5322.0"
                     /alternative_weights="5340.0"
                     /alternative_weights="5358.0"
                     /alternative_weights="5376.0"
     gene            complement(8428..8631)
                     /locus_tag="SBI_06990"
     CDS             complement(8428..8631)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_ha"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SBI_06990"
                     /product="hypothetical protein"
                     /protein_id="ADI10110.1"
                     /sec_met_domain="mature_ha (E-value: 3e-08, bitscore: 21.6,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHQEFEGDISLVEELAEQDLHDAAQHGGTISPIDTVKIIFRVSSH
                     FGGNGGFGLFCTLSKECQPNCN"
     CDS_motif       complement(8491..8631)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MHQEFEGDISLVEELAEQDLHDAAQHGGTISPIDTVKIIFRVSSHFG"
                     /locus_tag="SBI_06990_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(8428..8490)
                     /prepeptide="core"
                     /locus_tag="SBI_06990_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-3.50"
                     /molecular_weight="2053.3"
                     /monoisotopic_mass="2051.9"
                     /core_sequence="GNGGFGLFCTLSKECQPNCN"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MHQEFEGDISLVEELAEQDLHDAAQHGGTISPIDTVKIIFR
                     VSSHFG"
                     /leader_location="[8490:8631](-)"
ORIGIN
        1 tcaccccacc ggctggcccg gccgtgcgaa ttccgaggtc atcgccaccg ggacgcggaa
       61 gaagccgtcg tcgtggttct gctccgcggc atgcagcagg gcgtcggtgt cgccgatgcc
      121 ggcccagccg agcatgtagc cgagcagcga ggtccggccg ggcgaccctg tgtagaaccc
      181 gttttcgcgg gccctggtca ggacgagctc ctggtattcg cgtgcccggg tcagcagcga
      241 gtcgtcgccg agcacgcgcc ccgtggcgat cagcgactgg acgacgccac tgctgccgtg
      301 gcagacggac aggtccaccg ggcgacgggc gggcagccgt gtcgccgcct cgaccagggt
      361 cgtcagccga cggccggaga gttcgtccgc ccggccggcg gagaacagga tctcggcgga
      421 ggccagcagc agaccggccg caccgttgca ccaccccgcg agcgacgttc ccgtaccgga
      481 cccggcccgt gcggtcagcc agtccgcgga ctccctggcc ggcgtctcgt cgccgaggac
      541 ccttccgata cgggcgcggg cccactggag ccccagctcg ccgtgtgcca ggtcgaacca
      601 cggggcggtc ctgggggcct ggaggtggcc caggaccaga tcgcgcaggc gtgcgagccg
      661 ggcttcgccg cacagggcgg cggggtcgtc gccggcctcc agacgggaca gcaggaccat
      721 cagcagaccg gccgggccat tgctcaggtc ggtctccagg tcgttcgacg cggaccgtgc
      781 gtcgatcttg tcgagaaggg atccgagcca gtcggtcggc gccgcctgcg gtcgggtgag
      841 cagcagggag gaggcgccgc tgaacacgga ttccggcgtc tggttcagcg tctcgccgta
      901 ctccgtgagc agttcgtcga gtccctggtc ggcgcggtgg tgaatggtcc ggaagccgtc
      961 gtccagggtg gtcgcccggt tgaggaaggc gatgatgccg cccatgtcgt ggaacgagac
     1021 gaagttgccc ggggtgatgg tccccgcggc ccgcccgggg ccgatgccgc ccagccagcc
     1081 ggtccgcggg ccgtccgggg tgtggagggt tacgccgagg tcggcgatcc gacgcgcgat
     1141 ctccggccac caggtgcccg ggcgggcgtg ccgcaggggc gccgcgaaca cgctgtgccg
     1201 ggacagcccg gccgggctgt cctcgccgaa cacctcgccg aagcactcct cgagcagata
     1261 gtggtggtag ccgtccggcc gggcggcgtt gagggtgacg ccacgccggg cgtggtccac
     1321 cgacgaggtc ttgaacacct ccgggaagcg cacccgcgag gtcgccaggt cggtggagtc
     1381 cgcccgcgag ctgaaatagg gcacgttccc cgtgttcata ccggcgcgct cctcgtcgcc
     1441 gatgtgactg gccaactcgg gtgtgaactg gccggagttc ccgagcagcc cgaacagccg
     1501 ctgggcctcc tcgggccggg cgaggtagga ggggtgcgtc gaggcgtcca tgaaccgggc
     1561 gtagaccagc gtcgcccgca gcacataccg gacgggcatg tcccgatggg cgtcgaggat
     1621 cgccgggatc gcaccgccgc gcacgaactc gagggcgtcg gagtagccgc tcagcacatc
     1681 ggagaagtag tcggtcgcgg acagcggcac gtcacccagc cgtggcaggt tggcatcctg
     1741 ccggtaggtg atggggtccc gcccgacgga catgccgtcg gtgccgatgt cgcggatcac
     1801 cgtcttggtc atcttggagg gctggtcgcc ggccacgccc acccccgcca tgaggacgtc
     1861 gacgggggag gccgggctca tcgaggggac gagcatcgtg gagacgaccg acagcttcat
     1921 gtgattgatc agcaggtgcg gaagcgtatc gttgccgacg cccgcgtccg gccgcaggat
     1981 ggtctcggtg tcgatcacgc agggattctc gccgtgggcc agcagattct cgtcgtgcag
     2041 atcggatgcc ccgatggcgg agaggatcgc gcacagcgcg ccgaaccggt agaagtaccg
     2101 ggcgggttga tcgggcgagg ccatcggctc cgacggaatg aactgctgcc agccgtggga
     2161 gccggacgtc accgacatgg gcaggcagtc ccgcagcgag aaggtgaggt aggggtcggc
     2221 tgcggtgtac aggtcccgta cgaaactgtc cgtcgccagg gcgcgcggct tgaagatcag
     2281 ccgcgtaccg ctggtcagcc gcaccccgat cacctggcgg ttgtcgttgt gcggatcgga
     2341 cccggtgagg aagaggtcct cgatcagctc cccggccgcc gcggtacggg gcgtcaggca
     2401 gtcggcccgc agcgcctcct ggtccgccat gtacgcggcg aacatatcgg tgtacgcgtc
     2461 aagggagttg tccaccagaa ccgtcagccg ctgccgcagc accgggaagg cgtcggcgat
     2521 gcgccggcag gtgccgggcg cgccgagatg cctgcggaac cgctgcagcg cctcgtcgac
     2581 gtcggccgtc atgggcagcc caaggccctc gcggaaggtg tggaactcgc cgatcagcgt
     2641 acggaaggag tggccctcga cggctgtcag cagctccccc cagatcgcgt ccagcaccgc
     2701 ctcgcggcgt tcggggcggg cggccggagc catcacctcg cgcagcgccg cgaccacgga
     2761 gctcctgggc acgaggtgct gatagaaggg gaggaaggcg gggatcgaga cgtccgggtc
     2821 ggggatcgag ccctccgggc cggtcgtgct ttccggtccc gacatcagga catcggggtc
     2881 actttccgcg tagagattca tgtagaccgg tcctccgttc agcaattcct cgtgtgtgcc
     2941 ctgttcgatg acgcgtccgc ggtcgagcac gtaaatgcgg tcggccgctt tgatggtcgc
     3001 cagccggtgg gcgatgatga tctgcgttgc gccgatgtcc tggatgatgt ccgtgacgcg
     3061 tcgctcgttg atggtgtcca gcgaggccgt cgcctcgtcg agcaccagaa tcgcgggccg
     3121 ctgcagcagg gtccgtacga tggcgagccg ctgccgctga ccgccggaga agttcgcgcc
     3181 catttccgag atcagcgtgt tgagccccat gggaaggtcc tggaggaagt cgaggattcc
     3241 gacgctctcg cagaattcac ggacttgttc ctcggggatg tcctgcccca tcgtcaggtt
     3301 ctccaggatc gtgcggttgt gcagatgcac ttcctggggg atgtatccga tggagcggcg
     3361 cagcgaatcc ttgtcgtatt cgctcatgtc ccgcccgccg aactcgatgg tgccgctcgt
     3421 cggttcgtac agcccgcaga tgatccgccc cagggtgctc ttaccggatc ccgaggggcc
     3481 gacgagggcg accttggccc cggccggaat gtcgatcgag acatcgctca gcaccgggtc
     3541 gctgtggcgc gtgtactgga agccgatgtc ggtcaggcgg atggaggtcg agggcagccg
     3601 cttgaggtcg ccgccgcgat cctccgaggg cgtctcggcg atgtcgttga gccgcgccat
     3661 gtagcgggac gcctcggcga actcggtgca ggtctggaac accgaggtcg acagcgcgaa
     3721 gtaggtgccg gacaccgtct gcaccgccat ggcctggccc agggagatct gcccgtggcc
     3781 gacgagatac aggctcgaca gcagcaggat cagcgggccg aacatctggg tggtggaggt
     3841 caggcccgag atccggccct gttgcaggcg catgcggtcc ttcatcgcct ggagcgacga
     3901 ggaatagacc ttgctccagc cggccaggaa ctcggcggcg taaccgccca tcttgatggt
     3961 gggcaccgag acgatcgcgt ccagctgggc cgactggctc ttgctcagct gggtcatctc
     4021 cgtgtccacg gactccagca cgcggacccg ggtcttcatc agatagaggg cattgaccag
     4081 ggacagtccc gccgctatca ggccgaggcg ccattcggcg atcagcagat agccgctgac
     4141 gcacaccagt gtcccgacgt ccagtacgcc ctgggccacg cgtgaggaga gcagatccct
     4201 gatcacattc acgctgttga gccggaacag aagttcgccg ggttgtcgcg tcgtgaagaa
     4261 cttgtacgga agggagagca gcttggagaa cgtctggatc atcagatgct cgcccatcat
     4321 cgccaccacg gacgagagaa cggcgactct gagcagatac agcgcgaaat acccgacggc
     4381 cacggcgatg atgaccgaaa ggaccgtggc caggtcgtcc agccctcccc agcgggcgta
     4441 ccggttgacc gaccattccg tcagcatcgg tatgccgagg gttcccgcgt atccaccgag
     4501 cgagagaaat cccaccatcg cgatccgcct gcgcgaaccc ttcgcgaaga gcgagacccc
     4561 gcgccactcc gccatgaact tccgtggcgt cggttcgaag tcggggccgg gctcggcgga
     4621 caccgcgata ccgctgaagc cggcctccag ttctttcctg ctgaggcgcc gccgcccgag
     4681 cgccgggtcc atgatgaccg ctctgcggcc gtcgaaccgt tcgaggacca cgaagtggtg
     4741 gtcctcccag tagaggatca ccggcgaggt gaacttcgcg agggccgaca cgtccttgac
     4801 gcggaagagc ttcgcgtcca tgcctcgttc ccgcaggaag cgggccagtt gggtggcgct
     4861 gaggccgtcc cggccggcct ccatgacctg ccgcgcgctg gatatctcct ccgcgcgccc
     4921 gtggtagcgc agcaccgcga cgcagcagca caggccgcat tcggtctggg ccacctgcgt
     4981 gatcgtcggt acccgccggt ccgggccgga ccggcggacg atggtgttcc acgtcatgtc
     5041 atcggccctc cgtactcagc tcgacggggg acagccgagc ggacggatcg aggcggttga
     5101 cgagatggat caccatcccg gcggtcccga cgaggaggct gttggtgtgc gcgtaccggc
     5161 tccgggtgtc accggccttg cgggtgaaca cttcggggcg cagccatgtc cgctcgacgt
     5221 ccgcgaccgc ggtgtgcagc gaatcctctg tgctcgccac ggtggagagg atgtcgtggt
     5281 tgccgagatc cccatggcac caggtcagat tgcgcccgaa accgcggtgg accaggttgc
     5341 ccacggccac cttccgcatg cgctccaccg actcgtcgcc ggtcatcgcg gtgtacgtcg
     5401 tgagggcgag ggcgatgccg gccgagccgt ggcaccagcc ggtcgagaag gacccggggg
     5461 cggccatgtt ggagtgccag tcgtcctcgg cggggtcgta gaagttccat agccggtcga
     5521 gcaggcgcga cagcgtggcc tcgacgccct ttcgctgctg ctcgggcaga cgcgggtagg
     5581 cccggctcag cgcgtggatg acaccgctga ccccgtgtcc gaagccggac tggtcgagta
     5641 cggggtggtg tgcgtccggc cgcagggcgt ccgtgagcat ggccgtcagc gtggcggccg
     5701 actcgccggc gttcggtccg ccgatggccg tcacacagga caggaccccc gccagcccgc
     5761 tgatcatgtc cagcggccgg tcagccgcag gtccagcggc cacgaggtcg agcacgtcgg
     5821 gcaccgccag ctgcgcggcg cggacccaat cgggctgtcc gagcagcctg cccgccgccg
     5881 ccagcgcgaa cagcatgccg gccgatccgg tgtaggccga gtagggcgcc gtctgcctgg
     5941 tgtccgatgt ctcgggcggt agatcgggca ggggatcgga caggccctcc gcgatggccg
     6001 agaagatctg tgtggcgagg ccgcggtagg cggggtggtc caggacgcgg ccggccgccg
     6061 ccagggccag cgcggggccg accctccccg tgtacaggtc gtatccgagc accgcgggcg
     6121 gccatggccg attggactgc gcggaggcga gcgggccgat ccaggtatgg ggaagatcgg
     6181 cgaaccggtc gggcagggcc gaggcgacca tggtgtcgca cagccgggtc acgaccccgg
     6241 cgagcgtgtc gcccggagtc cccgtcggcc gcccgcccgc tgtggcgccg ggcagcgggg
     6301 cgtcgaagtc gtcggccgag gtcagatggt tgtcggggaa gcggcaggag aaggcggagt
     6361 gaatgagccg cagctgctcg cccacctcga actcggtcag ggcggcaacc ttggccgcgg
     6421 ccagatcgag tggcgatgtg ggcaacgccg cctcgacctc ggtgccctcg ccgtccgtga
     6481 gggccacgtc cgtcgcggca acggtgaagt aggggatgtc gcgccccgcg agctgccgca
     6541 actcggagcg gacgatcgcg cgcgcggagg gctcgctgac gatggccacc cgtttcagcg
     6601 ccgccagata ggacgcgggg tcactcatgg cccgggcgct cgacgccgtg cgcagcgtct
     6661 gcgtgtacaa catcgtgggg ttgtggaggt agcgcacgcg cacgccggca gcggccttgc
     6721 gcaccagcgc cgtccagcgt cccttgtccg acagcaccgc gcggcacacc cggacgaatc
     6781 cggccgccat cccctcgccc agtgcgtgga cctcgtcctg gctcagcgtc cccacgaccg
     6841 tctgccgttc ctccgccgcc tgcttgctca gcgtcagtcg cacctggtcg gtgaacgggg
     6901 cgtcgaactg gagggacttg aacggcgact cgccgctgcc ctgttcgccg aggaagccga
     6961 ggtcgacata gccggagtcc tcgtcccggc cggccatgag caggggcagg atcccgatgc
     7021 cgtagaccga ctgcgcgatc ctgtgatggg cattgtccgg tgtctcctcg ggcgtaccga
     7081 ggtggagccg gacgggctgg aggatcgtct ccaggtcgat cggcaccggg ccgcggcgcg
     7141 tggcgacgac attctcgaga tgcatgtcct gcgcgttgag cagatacatc acggcggcga
     7201 gctcgccgct ggccgccatg aaccgtgcgg agatgtcgga gacgtcctcc gctgccacgt
     7261 actccacata tccatagccg gcccgctcca ggaccttggc ggcgaccagg gaggtgccca
     7321 gccagtcgtt ggcctcgcgg gcgatgacgg cgtacgcggc ctcgcaggac acgtcgcgcg
     7381 gcttgtaggc cagcttcctg ccggaccgga actccaggac ggacacgctg cggccatggt
     7441 ggtgggcgtc gccgtcggcg aatccgcagg agacgacctg gtcctccgca tcgatgccga
     7501 aagtggcggc gatggcgtcc cggtctgctg cgagccgcag gcacagctcc cggaagttgt
     7561 gcgcctcgta ggccagcacg acgcgtgtga catcccgcag tacgggaaac gacaggccgc
     7621 tcagttcctc gaaggaaccg cggaccgact ggtcgacgaa ataccggtac cgcagggccg
     7681 ggtccgcgcc cacgagccga ccgtggcggc gtgcgtggtt gacggcgacg accagcggcc
     7741 tggtgtacac cgcgcgcagc cgggcctcgg cacgctccag caactgccgt agtaggtccg
     7801 ggggatcggc gaacagcggc gcgaacgggg cggtcggatc ggggtccgcg agcgaggcgg
     7861 cggcgttcct gatgattttc tggaacgggt aacgctcagg ggccgcgcac cactgtgtca
     7921 cagcgtcgcg cgtggcggcg ctccgtgata cggcggcccg ggggatttcg atgcctcgga
     7981 aaaattcgag ggtctgtgcg tcgaattgcg ccagcggcgc gatggaatcc gagatctgag
     8041 cgtcgctcag ttccggatag tacggggctg tggttttcac gactcgtaat actccatatc
     8101 caggtggacg cgccggaatt cggcggacgg ggcggaaccg ggggcccgtc cgaaggcccc
     8161 cgttccgccg cgatgcgcta cgtactcacc gaaccggacg cccggcgctc agcacctgct
     8221 ggtgcacttg gtggtggggc agaacgcggc ggagaccccg acggcgaccc cggcctggat
     8281 ggcaacgatg ccgaggatca agccggtgac ggcgggatcg gtgcccccgt ggacgtctgc
     8341 ctcgctcaat tcgacgagct cggcctcgtc ccatgcgccg aggatgtccg tgtccttctg
     8401 catagcaatt tcctcctctg gctttcgtca gttgcagttg ggctggcatt ccttggacag
     8461 ggtgcagaac aatccgaacc cgccgttccc tccgaagtgc gaactgacgc ggaagatgat
     8521 tttgactgtg tcgatcgggc tgatcgtgcc gccgtgctgt gcggcgtcgt gcaagtcctg
     8581 ttccgcgagc tcttccacga gggaaatatc tccctcgaat tcctgatgca t
//
