BGC0001230: salinamide A biosynthetic gene cluster from Streptomyces sp. CNB091
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Location: 1 - 48,212 nt. (total: 48,212 nt).
This entry is originally from NCBI GenBank BK009377.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001230
Short description salinamide A biosynthetic gene cluster from Streptomyces sp. CNB091
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "You can also contact Lauren Ray (laray@ucsd.edu) with regards to this MIBiG submission. \n"
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
BK009377.1
via Knock-out studies, Enzymatic assays, Heterologous expression
Compounds
  • salinamide A
  • salinamide B
  • salinamide C
  • salinamide D
  • salinamide E
  • salinamide F
  • desmethylsalinamide C
  • desmethylsalinamide E
Species Streptomyces sp. CNB091 [taxonomy]
References
Chemical products information
salinamide A Evidence: NMR, Mass spectrometry, X-ray crystallography, Total synthesis
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C51H69N7O15
Chemical database entries
NPAtlas
ChemSpider
salinamide B Evidence: NMR, Mass spectrometry
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C51H70Cl1N7O15
Chemical database entries
NPAtlas
ChemSpider
salinamide C Evidence: NMR, Mass spectrometry
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C52H73N7O14
Chemical database entries
NPAtlas
ChemSpider
salinamide D Evidence: NMR, Mass spectrometry
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C50H67N7O15
Chemical database entries
PubCHEM
ChemSpider
salinamide E Evidence: NMR, Mass spectrometry
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C43H62N6O12
Chemical database entries
NPAtlas
ChemSpider
salinamide F Evidence: NMR, Mass spectrometry
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C51H71N7O16
desmethylsalinamide C Evidence: Mass spectrometry
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C51H71N7O14
desmethylsalinamide E Evidence: Mass spectrometry
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C42H60N6O12
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • DAB41471.1
  • sln1
 1796 - 3115 (-) hydroxyphenylglycine aminotransferase
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    • DAB41472.1
    • sln2
    		 3115 - 4218 (-) 2-hydroxy-acid oxidase
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      • DAB41473.1
      • sln3
      		 4215 - 5297 (-) 4-hydroxyphenylpyruvate dioxygenase
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        • DAB41474.1
        • sln4
        		 5333 - 6367 (-) methyltransferase
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          • DAB41475.1
          • sln5
          		 6364 - 6582 (-) MbtH-like protein
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            • DAB41476.1
            • sln6
            		 6623 - 10618 (-) nonribosomal peptide synthetase
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              • DAB41477.1
              • sln7
              		 10615 - 22797 (-) nonribosomal peptide synthetase
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                • DAB41478.1
                • sln8
                		 22794 - 27458 (-) nonribosomal peptide synthetase
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                  • DAB41479.1
                  • sln9
                  		 27455 - 31411 (-) nonribosomal peptide synthetase
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                    • DAB41480.1
                    • sln10
                    		 31768 - 32976 (+) cytochrome P450
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                      • DAB41481.1
                      • sln11
                      		 32973 - 33914 (+) beta-ketoacyl-ACP synthase III (KSIII)
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                        • DAB41482.1
                        • sln12
                        		 33925 - 34167 (+) acyl carrier protein
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                          • DAB41483.1
                          • sln13
                          		 34234 - 36951 (-) LuxR regulatory protein
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                            • DAB41484.1
                            • sln14
                            		 37027 - 44697 (-) nonribosomal peptide synthetase/polyketide synthase type I
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                              Biosynthesis information

                              Biosynthetic modules

                              Name
                              2
                              Type
                              nrps-type1
                              Genes
                              sln14
                              Substrates
                              threonine (evidence: Structure-based inference, Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation, adenylation
                              Name
                              3
                              Type
                              nrps-type1
                              Genes
                              sln8
                              Substrates
                              valine, isoleucine (evidence: Structure-based inference, Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation, epimerase
                              Name
                              4
                              Type
                              nrps-type1
                              Genes
                              sln7
                              Substrates
                              4-hydroxyphenylglycine (evidence: Structure-based inference, Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (DCL), adenylation
                              Name
                              5
                              Type
                              nrps-type1
                              Genes
                              sln7
                              Substrates
                              phenylalanine (evidence: Structure-based inference, Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation, methyltransferase
                              Name
                              6
                              Type
                              nrps-type1
                              Genes
                              sln7
                              Substrates
                              threonine (evidence: Structure-based inference, Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation, epimerase
                              Name
                              7
                              Type
                              nrps-type1
                              Genes
                              sln6
                              Substrates
                              serine (evidence: Structure-based inference, Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (DCL), adenylation
                              Name
                              8
                              Type
                              nrps-type1
                              Genes
                              sln9
                              Substrates
                              glycine (evidence: Structure-based inference, Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation, adenylation
                              Name
                              1
                              Type
                              pks-modular-starter
                              Genes
                              sln14
                              Substrates
                              Integrated Monomers
                              Domains
                              acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                              Annotation changelog

                              Entry version: 4

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-10-07
                              Update chemical activity to schema version 2.11
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 3

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-09-15
                              Add additional publication
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Removed incorrect polyketide module information
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Corrected NRP module activity
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Corrected NRP gene names
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Updated bioactivity data
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Updated NRP substrate specificities
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 2

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2019-10-16
                              Migrated from v1.4
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2019-10-16
                              Updated compound(s) information (NPAtlas curation)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 1

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2015-12-24
                              Submitted
                              • (ID: UJYWT4SZRXDY2IOPYAIF6MII)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              Detailed domain annotation
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