BGC0001230 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001230 (Streptomyces sp. CNB091)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	48,212	salinamide A/salinamide B/salinamide C/salinamide D/salinamide E/salinamide F/desmethylsalinamide C/desmethylsalinamide E	NRPS:Type I+PKS:Type I	100%

BGC0001230 - Region 1 - NRPS,T1PKS

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Location: 1 - 48,212 nt. (total: 48,212 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 48,213
Label		BGC0001230

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001230	1.61	NRP, Polyketide	salinamide A, salinamide B, salinamide C, salinamide D, salinamide E, salinamide F, desmethylsalinamide C, desmethylsalinamide E	Streptomyces sp. CNB091
BGC0000426	0.83	NRP	sevadicin	Paenibacillus larvae
BGC0001050	0.80	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0001185	0.75	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0000465	0.73	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000401	0.71	NRP	paenibactin	Paenibacillus elgii B69
BGC0002075	0.66	NRP, Alkaloid	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002099	0.65	RiPP	biarylite-YFH	Planomonospora sp. ID107089
BGC0002084	0.64	RiPP	biarylitide YYH, biarylitide YFH	Planomonospora sp. ID82291
BGC0002017	0.63	NRP	potensibactin	Nocardiopsis potens DSM 45234

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001230	1.00	NRP, Polyketide	salinamide A, salinamide B, salinamide C, salinamide D, salinamide E, salinamide F, desmethylsalinamide C, desmethylsalinamide E	Streptomyces sp. CNB091
BGC0001050	0.74	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000426	0.73	NRP	sevadicin	Paenibacillus larvae
BGC0000465	0.72	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0001185	0.71	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0000401	0.70	NRP	paenibactin	Paenibacillus elgii B69
BGC0000416	0.70	NRP	rhabdopeptide 1, rhabdopeptide 2, rhabdopeptide 3, rhabdopeptide 4	Xenorhabdus nematophila ATCC 19061
BGC0002017	0.69	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0001434	0.68	NRP	nematophin	Xenorhabdus budapestensis
BGC0002437	0.67	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 48212:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - Thr) + (Gly) + (D-X) + (Hpg - NMe-Phe - D-Thr) + (Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

sln6

Search NORINE for peptide: strict or relaxed

AMP-binding (470..880): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHVSLIDK
Nearest Stachelhaus code(s):: Ser DVWHVSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

sln7

Search NORINE for peptide: strict or relaxed

AMP-binding (466..863): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIYMWGLLGK
Nearest Stachelhaus code(s):: Hpg DIYMWGLLGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1526..1897): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTVAAVCK
Nearest Stachelhaus code(s):: Phe DAWTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2959..3370): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWSVGMVHK
Nearest Stachelhaus code(s):: Thr DFWSVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

sln8

AMP-binding (482..874): X

nrpys: Ile, Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYFWGVTFK
Nearest Stachelhaus code(s):: Ile or Val DAYFWGVTFK (100% 8Å match); Val DAYFWGVTFK (88% 8Å match); Leu DAYFWGVTFK (88% 8Å match); Ile DAYFWGVTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

sln9

Search NORINE for peptide: strict or relaxed

AMP-binding (461..861): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code(s):: Gly DILQLGVIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

sln14

Search NORINE for peptide: strict or relaxed

PKS_AT (202..499): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.8
Ethylmalonyl-CoA: 194.8
Methoxymalonyl-CoA: 161.2
2-Methylbutyryl-CoA: 144.4
trans-1,2-CPDA: 138.2
Propionyl-CoA: 109.3
Malonyl-CoA: 101.4
CHC-CoA: 90.0
Benzoyl-CoA: 66.1
Isobutyryl-CoA: 63.7
fatty_acid: 38.5
Acetyl-CoA: 36.5
inactive: 32.8
3-Methylbutyryl-CoA: 23.7

AMP-binding (1950..2365): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)