BGC0001230: salinamide A biosynthetic gene cluster from Streptomyces sp. CNB091
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Location: 1 - 48,212 nt. (total: 48,212 nt).
This entry is originally from NCBI GenBank BK009377.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001230
Short description salinamide A biosynthetic gene cluster from Streptomyces sp. CNB091
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "You can also contact Lauren Ray (laray@ucsd.edu) with regards to this MIBiG submission. \n"
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide (Macrolide)
Loci NCBI GenBank: BK009377.1
Compounds
  • salinamide A
  • salinamide B
  • salinamide C
  • salinamide D
  • salinamide E
  • salinamide F
  • desmethylsalinamide C
  • desmethylsalinamide E
Species Streptomyces sp. CNB091 [taxonomy]
References
Chemical products information
salinamide A
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C51H69N7O15
Chemical database entries
NPAtlas
ChemSpider
salinamide B
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C51H70Cl1N7O15
Chemical database entries
NPAtlas
ChemSpider
salinamide C
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C52H73N7O14
Chemical database entries
NPAtlas
ChemSpider
salinamide D
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C50H67N7O15
Chemical database entries
PubCHEM
ChemSpider
salinamide E
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C43H62N6O12
Chemical database entries
NPAtlas
ChemSpider
salinamide F
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C51H71N7O16
desmethylsalinamide C
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C51H71N7O14
desmethylsalinamide E
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C42H60N6O12
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • DAB41471.1
  • sln1
 1796 - 3115 (-) hydroxyphenylglycine aminotransferase
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  • DAB41472.1
  • sln2
 3115 - 4218 (-) 2-hydroxy-acid oxidase
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  • DAB41473.1
  • sln3
 4215 - 5297 (-) 4-hydroxyphenylpyruvate dioxygenase
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  • DAB41474.1
  • sln4
 5333 - 6367 (-) methyltransferase
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  • DAB41475.1
  • sln5
 6364 - 6582 (-) MbtH-like protein
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  • DAB41476.1
  • sln6
 6623 - 10618 (-) nonribosomal peptide synthetase
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  • DAB41477.1
  • sln7
 10615 - 22797 (-) nonribosomal peptide synthetase
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  • DAB41478.1
  • sln8
 22794 - 27458 (-) nonribosomal peptide synthetase
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  • DAB41479.1
  • sln9
 27455 - 31411 (-) nonribosomal peptide synthetase
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  • DAB41480.1
  • sln10
 31768 - 32976 (+) cytochrome P450
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  • DAB41481.1
  • sln11
 32973 - 33914 (+) beta-ketoacyl-ACP synthase III (KSIII)
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  • DAB41482.1
  • sln12
 33925 - 34167 (+) acyl carrier protein
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  • DAB41483.1
  • sln13
 34234 - 36951 (-) LuxR regulatory protein
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  • DAB41484.1
  • sln14
 37027 - 44697 (-) nonribosomal peptide synthetase/polyketide synthase type I
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Polyketide-specific information
Subclass Macrolide
Starter unit Methylmalonyl-CoA
Cyclic? no
Polyketide-synthases
Genes Properties Modules
sln14 Synthase subclass: Modular type I
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: sln14
Core domains: Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
Thioesterase genes
  • sln6 (Type I)
  • sln9 (Type I)
NRP-synthases
Gene Modules
sln14
Module 2
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Unknown
sln8
Module 3
Specificity: valine / isoleucine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: LCL
sln7
Module 4
Specificity: 4-hydroxyphenylglycine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: DCL
Module 5
Specificity: phenylalanine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: LCL
Modification domains: Methylation
Module 6
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: LCL
sln6
Module 7
cyclizing TE

Specificity: serine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: DCL
sln9
Module 8
intermolecular transesterifying TE

Specificity: glycine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Unknown
Annotation changelog
MIBiG version Submitter Notes
1.2
  • Hidden contributor (ID: UJYWT4SZRXDY2IOPYAIF6MII, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Add additional publication
  • Removed incorrect polyketide module information
  • Corrected NRP module activity
  • Corrected NRP gene names
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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