BGC0001231: microsclerodermin biosynthetic gene cluster from Jahnella sp. MSr9139
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Location: 1 - 62,268 nt. (total: 62,268 nt).
This entry is originally from NCBI GenBank KF657739.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001231
Short description microsclerodermin biosynthetic gene cluster from Jahnella sp. MSr9139
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "module 1 can have 1 or 2 iterations, both derivatives are found. Module 3 has 2 iterations as inferred from molecule structure."
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
KF657739.1
1 - 62268
via Knock-out studies
Compounds
  • microsclerodermin
Species Jahnella sp. MSr9139 [taxonomy]
References
Chemical products information
microsclerodermin Evidence:
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C40H49Cl1N8O12
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AHB82060.1
  • mscK
 1 - 1350 (-) MFS transporter
  • Transport
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  • AHB82061.1
  • mscJ
 3006 - 3794 (+) Thioesterase II
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    • AHB82062.1
    • mscA
    		 4124 - 14731 (+) polyketide synthase
    • Scaffold biosynthesis
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    • AHB82063.1
    • mscB
    		 14718 - 17354 (+) polyketide synthase
    • Scaffold biosynthesis
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    • AHB82064.1
    • mscC
    		 17341 - 21990 (+) polyketide synthase
    • Scaffold biosynthesis
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    • AHB82065.1
    • mscD
    		 22000 - 24546 (+) polyketide synthase
    • Scaffold biosynthesis
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    • AHB82066.1
    • mscM
    		 24583 - 25899 (+) Fe(II)/α-ketoglutarate dependent oxygenase
    • Tailoring
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    • AHB82067.1
    • mscN
    		 25881 - 26714 (-) methyltransferase
    • Tailoring
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    • AHB82068.1
    • mscE
    		 26747 - 27907 (-) Putative Amidohydrolase
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      • AHB82069.1
      • mscF
      		 28287 - 34856 (+) non ribosomal peptide synthetase
      • Scaffold biosynthesis
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      • AHB82070.1
      • mscG
      		 34858 - 39393 (+) polyketide synthase
      • Scaffold biosynthesis
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      • AHB82071.1
      • mscH
      		 39472 - 51792 (+) non ribosomal peptide synthetase
      • Scaffold biosynthesis
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      • AHB82072.1
      • mscI
      		 51789 - 60668 (+) non ribosomal peptide synthetase/polyketide synthase
      • Scaffold biosynthesis
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      • AHB82073.1
      • mscL
      		 60661 - 62268 (-) tryptophan halogenase
      • Tailoring
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      Biosynthesis information

      Biosynthetic modules

      Name
      5
      Type
      nrps-type1
      Genes
      mscF
      Substrates
      asparagine (evidence: Structure-based inference)
      Integrated Monomers
      Domains
      condensation (LCL), adenylation
      Name
      7
      Type
      nrps-type1
      Genes
      mscH
      Substrates
      glycine (evidence: Structure-based inference)
      Integrated Monomers
      Domains
      condensation (LCL), adenylation, methyltransferase
      Name
      8
      Type
      nrps-type1
      Genes
      mscH
      Substrates
      tryptophan (evidence: Structure-based inference)
      Integrated Monomers
      Domains
      condensation (LCL), adenylation, epimerase
      Name
      9
      Type
      nrps-type1
      Genes
      mscH
      Substrates
      glycine (evidence: Structure-based inference)
      Integrated Monomers
      Domains
      condensation (DCL), adenylation
      Name
      10
      Type
      nrps-type1
      Genes
      mscI
      Substrates
      glycine (evidence: Structure-based inference)
      Integrated Monomers
      Domains
      condensation (LCL), adenylation
      Name
      1
      Type
      pks-modular
      Genes
      mscA
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, dehydratase, ligase, carrier (ACP)
      Name
      2
      Type
      pks-modular
      Genes
      mscB
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase
      Name
      3
      Type
      pks-modular
      Genes
      mscC
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      4
      Type
      pks-modular
      Genes
      mscD
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase
      Name
      5
      Type
      pks-trans-at-starter
      Genes
      mscF
      Substrates
      Integrated Monomers
      Domains
      carrier (ACP)
      Name
      6
      Type
      pks-modular
      Genes
      mscG
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Name
      11
      Type
      pks-modular
      Genes
      mscI
      Substrates
      Integrated Monomers
      Domains
      acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
      Annotation changelog

      Entry version: 4

      Date
      Changes
      Submitters
      Reviewers
      2022-10-07
      Update chemical activity to schema version 2.11
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

      Entry version: 3

      Date
      Changes
      Submitters
      Reviewers
      2022-09-15
      Corrected NRP module activity
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      2022-09-15
      Fixed incorrect gene identifiers
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      2022-09-15
      Removed ketoreductase stereochemistry annotation from modules without ketoreductases
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      2022-09-15
      Changed amino acid substrates to lower case
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

      Entry version: 2

      Date
      Changes
      Submitters
      Reviewers
      2019-10-16
      Migrated from v1.4
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

      Entry version: 1

      Date
      Changes
      Submitters
      Reviewers
      2015-12-24
      Submitted
      • (ID: RZRTXUKFH6LMF4VTOZ22YQ5Y)
      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
      Detailed domain annotation
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