BGC0001231: microsclerodermin biosynthetic gene cluster from Jahnella sp. MSr9139
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Location: 1 - 62,268 nt. (total: 62,268 nt).
This entry is originally from NCBI GenBank KF657739.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001231
Short description microsclerodermin biosynthetic gene cluster from Jahnella sp. MSr9139
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "module 1 can have 1 or 2 iterations, both derivatives are found. Module 3 has 2 iterations as inferred from molecule structure."
Biosynthetic class(es)
  • NRP
  • Polyketide (Aryl polyene)
Loci NCBI GenBank: KF657739.1
Compounds
  • microsclerodermin
Species Jahnella sp. MSr9139 [taxonomy]
References
Chemical products information
microsclerodermin [synonyms: pedein]
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C40H49Cl1N8O12
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AHB82060.1
  • mscK
 1 - 1350 (-) MFS transporter
  • Transport
Sequence-based prediction
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  • AHB82061.1
  • mscJ
 3006 - 3794 (+) Thioesterase II
  • Other enzymatic
Sequence-based prediction
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  • AHB82062.1
  • mscA
 4124 - 14731 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82063.1
  • mscB
 14718 - 17354 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82064.1
  • mscC
 17341 - 21990 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82065.1
  • mscD
 22000 - 24546 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82066.1
  • mscM
 24583 - 25899 (+) Fe(II)/α-ketoglutarate dependent oxygenase
  • Tailoring (Hydroxylation)
Sequence-based prediction
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  • AHB82067.1
  • mscN
 25881 - 26714 (-) methyltransferase
  • Tailoring (Methylation)
Sequence-based prediction
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  • AHB82068.1
  • mscE
 26747 - 27907 (-) Putative Amidohydrolase
  • Unknown
Sequence-based prediction
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  • AHB82069.1
  • mscF
 28287 - 34856 (+) non ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82070.1
  • mscG
 34858 - 39393 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82071.1
  • mscH
 39472 - 51792 (+) non ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82072.1
  • mscI
 51789 - 60668 (+) non ribosomal peptide synthetase/polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82073.1
  • mscL
 60661 - 62268 (-) tryptophan halogenase
  • Tailoring (Halogenation)
Sequence-based prediction
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Polyketide-specific information
Subclass Aryl polyene
Cyclic? yes
Release type
  • Macrolactamization
Polyketide-synthases
Genes Properties Modules
mscG
+
mscF
+
mscD
+
mscC
+
mscB
+
mscI
+
mscA 		Synthase subclass: Modular type I
Thioesterases:
  • mscI (Type I)
Module 1
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mscA
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, CoA-ligase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
Module 2
Genes: mscB
Core domains: Ketosynthase, Acyltransferase
Non-canonical activity:
  • Skipped
Evidence for non-canonical activity:
  • Structure-based inference
Module 3
Genes: mscC
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
Module 4
Genes: mscD
Core domains: Ketosynthase, Acyltransferase
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
Module 5
Genes: mscF
Core domains: Thiolation (ACP/PCP)
Module 6
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mscG
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 11
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mscI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
NRP-specific information
Subclass N/A
Cyclic? yes
Release type
  • Macrolactamization
NRP-synthases
Gene Modules
mscA
Module 0
mscF
Module 5
Specificity: asparagine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
mscH
Module 7
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Modification domains: Methylation
Module 8
Specificity: tryptophan
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 9
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
mscI
Module 10
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.2
  • Hidden contributor (ID: RZRTXUKFH6LMF4VTOZ22YQ5Y, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Fixed incorrect gene identifiers
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Changed amino acid substrates to lower case
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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