BGC0001235 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001235 (Polyangium spumosum)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	50,952	antalid	NRPS:Type I+PKS	100%

BGC0001235 - Region 1 - NRPS,T1PKS

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Location: 1 - 50,952 nt. (total: 50,952 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 50,953
Label		BGC0001235

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001235	1.85	NRP, Polyketide	antalid	Polyangium spumosum
BGC0002437	0.56	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0002226	0.52	NRP	acu-dioxomorpholine B, acu-dioxomorpholine A	Aspergillus aculeatus ATCC 16872
BGC0001873	0.50	NRP	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0002135	0.50	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.50	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001132	0.49	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833	0.49	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128	0.48	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001844	0.48	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001235	1.00	NRP, Polyketide	antalid	Polyangium spumosum
BGC0002437	0.62	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0002226	0.61	NRP	acu-dioxomorpholine B, acu-dioxomorpholine A	Aspergillus aculeatus ATCC 16872
BGC0001873	0.59	NRP	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0002135	0.58	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.58	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001132	0.58	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833	0.58	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128	0.57	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001844	0.57	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 50952:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - Me-ccmal - Me-ccmal) + (Val - Cys - D-Phe)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 46663:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - Me-ccmal - Me-ccmal) + (Val - Cys - D-Phe)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 6677 - 50952:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - Me-ccmal - Me-ccmal) + (Val - Cys - D-Phe)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

antA

PKS_AT (672..968): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%; Methoxymalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 103.7
Methoxymalonyl-CoA: 92.1
Ethylmalonyl-CoA: 77.0
Malonyl-CoA: 74.6
2-Methylbutyryl-CoA: 69.1
Propionyl-CoA: 64.3
Isobutyryl-CoA: 61.7
trans-1,2-CPDA: 60.4
CHC-CoA: 53.6
Acetyl-CoA: 50.8
fatty_acid: 36.3
Benzoyl-CoA: 35.1
3-Methylbutyryl-CoA: 28.9
inactive: 16.2

PKS_AT (1096..1396): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.2
Ethylmalonyl-CoA: 200.9
Methoxymalonyl-CoA: 171.8
2-Methylbutyryl-CoA: 153.8
trans-1,2-CPDA: 136.1
CHC-CoA: 109.2
Malonyl-CoA: 104.6
Propionyl-CoA: 103.8
Isobutyryl-CoA: 95.0
Benzoyl-CoA: 62.5
Acetyl-CoA: 57.6
3-Methylbutyryl-CoA: 33.5
inactive: 33.0
fatty_acid: 30.3

PKS_AT (2124..2429): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 225.6
inactive: 128.4
Methoxymalonyl-CoA: 69.2
Methylmalonyl-CoA: 54.1
Isobutyryl-CoA: 32.0
Propionyl-CoA: 24.5
Acetyl-CoA: 21.6
Benzoyl-CoA: 11.6
2-Methylbutyryl-CoA: 11.1
Ethylmalonyl-CoA: 3.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3608..3905): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.5
Ethylmalonyl-CoA: 199.0
Methoxymalonyl-CoA: 176.2
2-Methylbutyryl-CoA: 158.9
trans-1,2-CPDA: 129.5
Propionyl-CoA: 111.8
Malonyl-CoA: 107.8
CHC-CoA: 95.3
Isobutyryl-CoA: 89.0
Acetyl-CoA: 60.5
Benzoyl-CoA: 59.3
fatty_acid: 32.5
3-Methylbutyryl-CoA: 32.1
inactive: 28.5

PKS_AT (5384..5681): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.8
Ethylmalonyl-CoA: 142.3
Methoxymalonyl-CoA: 133.3
Isobutyryl-CoA: 114.9
Malonyl-CoA: 95.0
Benzoyl-CoA: 93.2
Propionyl-CoA: 84.8
trans-1,2-CPDA: 82.5
2-Methylbutyryl-CoA: 80.3
Acetyl-CoA: 71.0
CHC-CoA: 63.6
3-Methylbutyryl-CoA: 51.8
inactive: 34.6
fatty_acid: 30.2

antB

Search NORINE for peptide: strict or relaxed

AMP-binding (532..926): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DFLQVGIIFK
Nearest Stachelhaus code(s):: Val DFLQVGIIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1584..1980): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLWNLSLIWK
Nearest Stachelhaus code(s):: Cys DLWNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3000..3398): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAATAGLIMK
Nearest Stachelhaus code(s):: Phe DAATAGLIMK (100% 8Å match)
Stachelhaus code match:: 100% (strong)