LOCUS       BGC0001238              7027 bp    DNA     linear   PLN 23-DEC-2010
DEFINITION  Emericella nidulans strain Glasgow G051 biotin biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0001238
VERSION     BGC0001238
KEYWORDS    .
SOURCE      Aspergillus nidulans
  ORGANISM  Aspergillus nidulans
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus; Aspergillus subgen. Nidulantes.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 00:13:16
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /db_xref="taxon:162425"
                     /mol_type="genomic DNA"
                     /note="biotin auxotroph mutant; teleomorph: Emericella
                     nidulans"
                     /organism="Aspergillus nidulans"
                     /strain="Glasgow G051"
     misc_feature    1..7027
                     /note="biotin biosynthesis gene cluster"
     subregion       1..7027
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001238"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7027
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     mRNA            complement(join(<108..434,502..644,694..>1462))
                     /gene="biF"
                     /product="8-amino-7-oxononanoate synthase"
     gene            complement(<108..>1462)
                     /gene="biF"
     CDS             complement(join(108..434,502..644,694..1462))
                     /EC_number="2.3.1.47"
                     /NRPS_PKS="Domain: Aminotran_1_2 (35-398). E-value:
                     1.7e-38. Score: 124.5. Matches aSDomain:
                     nrpspksdomains_biF_Aminotran_1_2.1"
                     /codon_start=1
                     /function="catalyzes the formation of
                     8-amino-7-oxononanoate from 6-carboxyhexanoyl-CoA and
                     L-alanine"
                     /gene="biF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109:8-amino-7-oxononanoate synthase (Score: 293.5;
                     E-value: 3.8e-89)"
                     /gene_kind="biosynthetic-additional"
                     /note="BiF; 7-keto-8-aminopelargonate synthetase; the
                     preantepenultimate step of biotin biosynthesis; absent in
                     Saccharomyces cerevisiae"
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="ACR44939.1"
                     /transl_table=1
                     /translation="MGDSPKCLRDSLREALRRREDKLCRRKLTILPSSSVDFSSNDFLS
                     LSTSPAYRARFLDILQQAPPLHPFASGGSRLLDGNSAYAEELENFIAAFHNAPSGLLFN
                     SGYDANVGVFSSIPQPGDLIVYDELIHASAHEGMRLSRAGKRIKFPHSSPDGLRAVLQA
                     EITADPRLLQGRRNVFIAFESVYSMDGDVAPIREFVEIVDQLLPYGNGYFLVDEAHATG
                     VFGPRGSGVVQELGLEDRMFVRVHTFGKALASHGAIVLCCADTRDYLINYARSLIYTTA
                     LGFPFLASIRAAYELLVEGKTEQLQHKLGQLIAHFRTGLDNLNHKDSSTFEVEHFTNSP
                     IFSLRSSVPRVLASVCQEQGYTVRAIMPPTVPAGKERVRVCLHAGNTVEEVDGLLETIA
                     TWLQRMEKQKARL"
     aSDomain        complement(join(153..434,502..644,694..1357))
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_biF_Aminotran_1_2.1"
                     /evalue="1.70E-38"
                     /label="biF_Aminotran_1_2.1"
                     /locus_tag="biF"
                     /protein_end="398"
                     /protein_start="35"
                     /score="124.5"
                     /tool="antismash"
                     /translation="VDFSSNDFLSLSTSPAYRARFLDILQQAPPLHPFASGGSRLLDGN
                     SAYAEELENFIAAFHNAPSGLLFNSGYDANVGVFSSIPQPGDLIVYDELIHASAHEGMR
                     LSRAGKRIKFPHSSPDGLRAVLQAEITADPRLLQGRRNVFIAFESVYSMDGDVAPIREF
                     VEIVDQLLPYGNGYFLVDEAHATGVFGPRGSGVVQELGLEDRMFVRVHTFGKALASHGA
                     IVLCCADTRDYLINYARSLIYTTALGFPFLASIRAAYELLVEGKTEQLQHKLGQLIAHF
                     RTGLDNLNHKDSSTFEVEHFTNSPIFSLRSSVPRVLASVCQEQGYTVRAIMPPTVPAGK
                     ERVRVCLHAGNTVEEVDGLLETI"
     CDS             join(2171..2358,2409..2502,2556..3443)
                     /EC_number="6.3.3.3"
                     /codon_start=1
                     /gene="biA1"
                     /note="BiA1; penultimate step of biotin biosynthesis"
                     /product="truncated dethiobiotin
                     synthetase/adenosylmethionine-8-amino-7-oxononanoate
                     aminotransferase"
                     /protein_id="ACR44941.1"
                     /transl_table=1
                     /translation="MAPVGAALWRSLRAHQVYGANTDVGKTIVSTFLCNAVNRLKNQGK
                     SAFLKPVSTGPLDEADDRHLQRHAPNTLTKCLYQFDEPVSPHIAAKTFAIPRDDEILSS
                     VHRTLSDWANDGVGFALVETAGGVHSPGPNGNSQADLYRPLRLPIILVADSRLGGISSS
                     ISAYESLLLRGYDVHSVLLFKDDYYQNHEYLGNYFRGKSIPLVPVPAPPRRPQEQDPDS
                     RARDLEALDKYYSSVTKSTDVVSLLDELVLKNKQRVEYLDEMASRAQKTIWYPFTQHHG
                     MAAKDITPIDSAYDDFFQTYVTADRSAQQGRLQATFDGSASWWTQGLGHGNPGLALSAA
                     YAAGRYGHVMFPGNIHEPALALAESLLKTVDNPRLQKSSIQTMAAQVWR"
     mRNA            join(<2171..2358,2409..2502,2556..3888,3941..4515,
                     4591..>4763)
                     /gene="biA1"
                     /note="may be subject to nonsense-mediated mRNA decay"
                     /product="truncated dethiobiotin
                     synthetase/adenosylmethionine-8-amino-7-oxononanoate
                     aminotransferase"
     gene            <2171..>4763
                     /gene="biA1"
     variation       3404^3405
                     /compare=AACD01000110.1
                     /compare=FJ430073.1
                     /gene="biA1"
                     /note="insertion restores activity of
                     adenosylmethionine-8-amino-7-oxononanoate aminotransferase
                     domain of the bifunctional BiDA protein"
                     /phenotype="biotin auxotroph"
                     /replace="g"
     mRNA            join(<5677..5886,5984..>6955)
                     /gene="biB"
                     /product="biotin synthase"
     gene            <5677..>6955
                     /gene="biB"
     CDS             join(5677..5886,5984..6955)
                     /EC_number="2.8.1.6"
                     /codon_start=1
                     /function="catalyzes the conversion of dethiobiotin to
                     biotin"
                     /gene="biB"
                     /note="BiB; dethiobiotin:sulfur sulfurtransferase; ultimate
                     step of the biotin biosynthesis"
                     /product="biotin synthase"
                     /protein_id="ACR44940.1"
                     /transl_table=1
                     /translation="MSVSFTRSFPRAFIRSYGTVQSSPTAASFASRIPPALQEAVAATA
                     PRTNWTRDEVQQIYETPLNQLTYAAAAVHRRFHDPSAIQMCTLMNIKTGGCSEDCSYCA
                     QSSRYSTGLKATKMSPVDDVLEKARIAKANGSTRFCMGAAWRDMRGRKTSLKNVKQMVS
                     GVREMGMEVCVTLGMIDADQAKELKDAGLTAYNHNLDTSREFYPTIITTRSYDERLKTL
                     SHVRDAGINVCSGGILGLGEADSDRIGLIHTVSSLPSHPESFPVNALVPIKGTPLGDRK
                     MISFDKLLRTVATARIVLPATIVRLAAGRISLTEEQQVACFMAGANAVFTGEKMLTTDC
                     NGWDEDRAMFDRWGFYPMRSFEKETNAATPQQHVDSVAHESEKNPAAPAAEAL"
ORIGIN
        1 cagtgccgga atcatggctt catgatttgc ctgacacatg taccgtacat gaaggacttc
       61 tgcgtcaatg gggatgtgat ttgtgggctc tgggtctgat gataatgcta taaccgagcc
      121 ttttgctttt ccatccgctg taaccacgta gcaatggtct caaggaggcc gtcgacctcc
      181 tccaccgtgt ttcccgcgtg caagcatacg cgtacacgtt ctttccctgc tggaacagtt
      241 ggcggcatga ttgcacggac ggtatatccc tgctcctggc atacgctggc gagcacacgg
      301 ggcacactgc tgcgaaggga aaatatgggt gagttcgtaa aatgttcaac ttcaaaggtc
      361 gacgagtcct tgtggttgag attgtccaac cccgtccgga agtgagcaat cagttgtccg
      421 agtttgtgct ggagctggat atttgttagc tttcccatag gcctgtaatg aaacgttgct
      481 tcctcaagcg aaggaccgta cctgctcagt ttttccctca acgagtagtt cataggcagc
      541 acgtattgaa gcaagaaagg gaaatcccag ggcagtggta tagatcaggc tgcgagcgta
      601 attgatcagg tagtctctag tatccgcaca acacaatacg attgctagca ggacagtcaa
      661 tatcgacacc caaagacaac gcgcgagact tacctccatg actagcgagt gctttaccga
      721 acgtatgtac ccggacgaac atacgatcct ccagtcccaa ctcttggact accccagaac
      781 cgcgtggtcc aaagacgccg gtcgcatgcg cctcatccac aaggaagtat ccgttaccgt
      841 aagggagaag ctggtccacg atctctacga actcacgaat aggcgcaaca tcgccgtcca
      901 tactgtagac ggattcaaag gcgatgaaaa cgttccggcg gccctgaaga agtcggggat
      961 ctgcagttat ttctgcttgc agtactgctc tcaggccatc gggagagcta tgagggaact
     1021 tgatacgctt ccctgctcgt gagagccgca tgccctcatg cgcgctggca tggatgagtt
     1081 catcgtatac tatcagatct ccgggttgcg ggatactcga gaaaacgcca acattggcat
     1141 catatccgga attgaacagt aggccactcg gggcattgtg gaacgcggca atgaagttct
     1201 ccagctcttc ggcataggct gaattgccgt ctaggaggcg ggaccctccg ctggcaaacg
     1261 ggtgcaacgg cggcgcttgt tgcaaaatat ccaaaaaccg cgctcgatag gctggcgatg
     1321 tggatagtga caaaaagtcg tttgatgaga aatcaaccga agacgaaggt agaatcgtca
     1381 gttttcgacg gcacagcttg tcttcccttc gacgtaacgc ctcgcgcagt gagtcacgaa
     1441 ggcattttgg agaatcgccc atctggacga ctgtggttcc aaggccaacg cttgccaata
     1501 ggcaattcag atagctggag acaagattca agtaaccgta aattcaaagg aggatctatt
     1561 ttttctctgg tgagattttg ccagtattaa tgctccagct caaatcacgc tctccaatct
     1621 ccacgagtct agaacgtctt gaagaccctg ctgtatggac ctgagacttg gggtagagcg
     1681 atcactgatg aagtagtgcc tgagtctcac cacattaaca catcatacgg agtagcacaa
     1741 ttcaattcgg cgggacttat ttcatctgtt gggatcaacc ggtgggggaa tggatccgac
     1801 gaacgtggct gggccgctgc ccggttgtgc cgctgcagtg actcggtgag caacgcagtt
     1861 ccctaggcaa gacaaaaggc ttaactcggg ggcctcggcc tggttcctgt ctcactgtgc
     1921 ctgttcctgc tagaacgact atgaacgcct aatgcaaagg gctcgagagt cgaaccacga
     1981 tcgggtttct ttccgcggta aagcgctgca gccttctgca accgaatctg tcggtcatca
     2041 ttcctgttcc ggtcgcagac gggtgccgaa aactgcttct aaagttcagt tggtgtttcg
     2101 gctctgtgat gcgttagcct ttcacattag cgactggttt ctgctgtttt gaaagtctag
     2161 attcaccatc atggcacctg ttggtgccgc gctctggcgc tccctccgcg cccaccaagt
     2221 atacggggcg aacactgacg tcggcaagac aattgtatca acatttcttt gcaatgctgt
     2281 taatcgtctg aaaaatcaag gaaagtcggc gtttttgaag ccggtctcga cggggccatt
     2341 ggatgaagcg gatgacaggt aaggccggga tgtcggcgag agagtgttac cgactcacgc
     2401 gaacacaggc acctgcaacg ccatgctccc aacactttga caaaatgcct ctatcaattt
     2461 gatgaacccg tcagcccaca tatcgcagct aagacgttcg ctgtcagaaa cccaaatcta
     2521 cttcgtccaa cataagcaca cactaacctc gacagattcc tcgagatgac gagatccttt
     2581 cctctgtcca caggaccctt tccgactggg ccaacgacgg agtcgggttc gctctggtag
     2641 aaacggccgg tggtgttcac tcgccgggcc ccaatgggaa ctcccaggcc gacctctacc
     2701 gacctcttcg gttgcccatc atcttggtcg ctgactcccg tcttggggga atctcatcct
     2761 ctatatccgc ctacgagtct ctcttgctcc gcggatacga tgtccactcc gtccttctat
     2821 ttaaggatga ctactatcag aatcatgagt acttggggaa ctacttccgg ggcaagagca
     2881 taccgcttgt cccggtgcct gcaccgcctc ggcgtccgca agagcaagac cccgactcgc
     2941 gagctcgcga tctggaagcg ttggacaagt attacagctc agttacaaaa agcacggatg
     3001 tggtctcgct cctagatgag cttgttttga aaaacaagca gcgtgtcgaa tacctcgacg
     3061 agatggcaag ccgagcacag aagacaatct ggtatccatt tacgcagcac cacggcatgg
     3121 cggcaaagga tatcactccc atcgattccg catacgatga tttcttccag acttacgtga
     3181 ctgcggatcg ttcggcacaa caaggcagac tccaagccac gttcgacgga tccgcatcgt
     3241 ggtggacaca gggactcggt cacggaaatc ccggccttgc tctatctgct gcgtatgccg
     3301 caggacgtta cgggcatgta atgttccccg ggaacattca tgagccggct ttggcgttgg
     3361 cggaatcgct cttgaagact gtggataacc ctcgcctaca gaagtcttct atacagacaa
     3421 tggcagcaca ggtatggagg tagctctcaa gatgggcctc cgcgctgcgt gcgaccggta
     3481 tggctgggat gcaagcaaag aacagatcaa cattctcggc ctcaaaggga gctaccatgg
     3541 cgacacgatc ggggttatgg actgctcaga gccgtcgacc tacaatcaaa gagttgagtg
     3601 gtacagaggc cggggtcact ggtttgattt ccctctggta aagatgtccc aaggggtctg
     3661 gcaggtcgag gtccccgcaa cactgcaagc gtcgcttggc gggaaccagc agttctcatc
     3721 actggatgca gtatttgatg tagaaagcag ggtccgctcg gatgctggac agcgctacag
     3781 gaagtatata ctagagacca ttgagcgcct ggttactcaa gaagggaaga agtttggcgc
     3841 cctaattatg gaacctatca ttctaggagc cggcggaatg ctcttctggt cagtatagat
     3901 ccttttttac aggaaagtta ctgacggacg accgttatag tgatcccctg ttccagcgat
     3961 gcctcgccga tgtcgtccgc ggtaaccccc agctcttcaa ccgaggccgg ttgacagaac
     4021 cgcagccgca gaccgacctc tcgtggtccg gtctccccgt catctttgac gaggttttca
     4081 cgggcctcta ccgtcttggc cgtaaatcct ctgcgtcatt tctaggtgtc aacccagaca
     4141 tcgctgtcaa cgcaaagctc cttaccggag gcctggtgcc gctctgcact accctagcca
     4201 gcaacgaaat cttcaacgcg tttacaagcc cggagaagcg agatgcgctc ttgcatgggc
     4261 acagctacac cgcgcatgcg gtcgggtgcc aagtagccct tgattccctt cggacaatga
     4321 acaatatgga cgaggacgga tcctggaacg acttcaagaa cgactggaaa caaccccatg
     4381 ccggcgacac cgcccgggtc tggagcgtct ggtcccacaa gctgctccac aacctttccc
     4441 acgccgaatc cgtcgatggc gtcttcgcta ttggtagcgt tctcagcatc tctctgaaag
     4501 atgcagaggg tgcaggtgag ttcccgactc tctctacaac aattctctcg ccgattctac
     4561 ccgctaacat gtgacacctc cactcattag gctatacatc cactgcggct aagggccttc
     4621 agacaagatt agcggctggt gggccgcagt ttaacgtcca ctctcgggtc ctcgggaatg
     4681 tgctttactt gatgtcaagc gtgacgtcca agcaggaaac actgcggaca attgagggca
     4741 tcctgcgcga ggcgctgctg taacttacaa cctaactagc atgtacatga ctaagctgta
     4801 ctctctgtgt aaacaagcgt cgtctcaccg gcaaccgtaa gaaaaaagtg tggacaccgg
     4861 accgccggat aggctggtgc gatcgtttga acccttgtag aatccagctc accgagtcac
     4921 cgggtctcga tatggagcca agatttacta ttgatgcccg ctctcgctgg ctgagtctgg
     4981 atcttacaga gacacactag ctcaggttca ctgagctccg gcctcgcagg cccggcgaca
     5041 atctaccccg ctattcgcca ggagcaccga cttccgaggt tattctaaat tggctcttag
     5101 tgagtctccc gcagatctgc agtttagcac cttgcttttt gctgtcgaat cagacccaag
     5161 gggataagat caggcactgg gctcgctgat tggtcggacc cgttctccat ctggaaatac
     5221 gcgatccact tccgtcagtc gttcactcga aatacgtaag acctcaggac ttgcgttgaa
     5281 ccagcgttgg ggtcaacact cctgcaaagg aagagtccgt ttgcagcgat agcaagcgaa
     5341 tcaagctaat caagccaatc aagcgatagg aagcgatagc aaataattgt agttgaggtc
     5401 aggcaatttg ctcagaatta ttggaccaat ttggaccaag ttgggccaat ttaggcccat
     5461 aataaattac aaagttacaa gagttcactc tgtagcggtt ttccaccgac atttcactcc
     5521 tctccaggcc aatgacgagt tacccttctt caggttccta ttgtggccca tgctcctctc
     5581 acctgcattt tcccctcccg ccgcatactt ttgatcaccc ctcgctgttt cccgcagtgt
     5641 caccttgata cactcctcca accacccgtc atcactatgt ctgtctcgtt tacgcggtcc
     5701 tttcctaggg ccttcataag gtcatatggc accgtccagt cgtcgcccac ggccgcttcc
     5761 tttgcgagca gaatcccccc ggctctccag gaggctgttg cagccactgc cccgcgcacc
     5821 aattggactc gcgatgaagt ccagcagatt tacgagaccc cgttgaatca attaacctac
     5881 gctgctgtat gtttccgatt tgaccgctgc ttataaatta ttctgtaacg cggatatttg
     5941 aatgaattgt tcgttggacc gaatgttgac tcatgctgaa taggccgctg tccaccgccg
     6001 ctttcatgac ccgtccgcaa tccaaatgtg cacgttgatg aacatcaaga ccggtggatg
     6061 cagtgaagat tgctcctact gtgcacagtc ttctcggtac agcactggcc tcaaggccac
     6121 caaaatgagc cccgtcgacg acgtcctcga gaaggcgagg attgccaaag cgaacgggag
     6181 cacgcgtttc tgtatgggag cggcgtggcg tgatatgcgg ggtcgtaaga cgagtttgaa
     6241 gaatgtcaag cagatggtat ctggcgttcg ggaaatggga atggaagtct gcgtcacact
     6301 aggcatgatt gatgctgatc aggctaagga gctgaaagat gccggcctga cagcctacaa
     6361 ccacaacctc gatacttcgc gcgaattcta ccccacaatc atcacaaccc gatcgtacga
     6421 cgaacgacta aagaccttgt ctcatgtccg tgatgcgggc attaacgtct gctctggtgg
     6481 tattctaggt cttggtgagg ctgactctga ccgcatcggc ctcatccaca cggtttcgtc
     6541 acttccctcg cacccggagt cttttcccgt caacgccttg gttccaatca agggtacccc
     6601 gttgggtgac aggaaaatga tctctttcga taagctcctc cgcactgtcg cgactgcacg
     6661 gatcgtcctt cccgcaacca tcgtccgcct cgccgccggc cgcatttctc tcacagagga
     6721 gcagcaggtt gcctgcttca tggctggtgc aaacgctgtc ttcactggag agaagatgtt
     6781 gactactgac tgcaacggct gggacgagga ccgcgccatg tttgaccgat ggggcttcta
     6841 ccccatgcgc agctttgaga aagagactaa cgctgccacc ccccagcagc atgttgactc
     6901 tgttgctcac gagtccgaga agaaccccgc tgcgccggcc gcagaagccc tatgataggg
     6961 ctctaaaact accccacccc cccagcctga tacgcttttc tccctgtccg tgattggtag
     7021 ggaagcg
//
