LOCUS       BGC0001239              7028 bp    DNA     linear   PLN 23-DEC-2010
DEFINITION  Emericella nidulans strain FGCS 4 biotin biosynthesis gene cluster,
            complete sequence.
ACCESSION   BGC0001239
VERSION     BGC0001239
KEYWORDS    .
SOURCE      Aspergillus nidulans
  ORGANISM  Aspergillus nidulans
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus; Aspergillus subgen. Nidulantes.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 00:13:24
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /db_xref="taxon:162425"
                     /mol_type="genomic DNA"
                     /note="teleomorph: Emericella nidulans"
                     /organism="Aspergillus nidulans"
                     /strain="FGCS 4"
     misc_feature    1..7028
                     /note="biotin biosynthesis gene cluster"
     subregion       1..7028
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001239"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7028
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     mRNA            complement(join(<108..434,502..644,694..>1462))
                     /gene="biF"
                     /product="8-amino-7-oxononanoate synthase"
     gene            complement(<108..>1462)
                     /gene="biF"
     CDS             complement(join(108..434,502..644,694..1462))
                     /EC_number="2.3.1.47"
                     /NRPS_PKS="Domain: Aminotran_1_2 (35-398). E-value:
                     1.7e-38. Score: 124.5. Matches aSDomain:
                     nrpspksdomains_biF_Aminotran_1_2.1"
                     /codon_start=1
                     /function="catalyzes the formation of
                     8-amino-7-oxononanoate from 6-carboxyhexanoyl-CoA and
                     L-alanine"
                     /gene="biF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109:8-amino-7-oxononanoate synthase (Score: 293.5;
                     E-value: 3.8e-89)"
                     /gene_kind="biosynthetic-additional"
                     /note="BiF; 7-keto-8-aminopelargonate synthetase; the
                     preantepenultimate step of biotin biosynthesis; absent in
                     Saccharomyces cerevisiae"
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="ACR44942.1"
                     /transl_table=1
                     /translation="MGDSPKCLRDSLREALRRREDKLCRRKLTILPSSSVDFSSNDFLS
                     LSTSPAYRARFLDILQQAPPLHPFASGGSRLLDGNSAYAEELENFIAAFHNAPSGLLFN
                     SGYDANVGVFSSIPQPGDLIVYDELIHASAHEGMRLSRAGKRIKFPHSSPDGLRAVLQA
                     EITADPRLLQGRRNVFIAFESVYSMDGDVAPIREFVEIVDQLLPYGNGYFLVDEAHATG
                     VFGPRGSGVVQELGLEDRMFVRVHTFGKALASHGAIVLCCADTRDYLINYARSLIYTTA
                     LGFPFLASIRAAYELLVEGKTEQLQHKLGQLIAHFRTGLDNLNHKDSSTFEVEHFTNSP
                     IFSLRSSVPRVLASVCQEQGYTVRAIMPPTVPAGKERVRVCLHAGNTVEEVDGLLETIA
                     TWLQRMEKQKARL"
     aSDomain        complement(join(153..434,502..644,694..1357))
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_biF_Aminotran_1_2.1"
                     /evalue="1.70E-38"
                     /label="biF_Aminotran_1_2.1"
                     /locus_tag="biF"
                     /protein_end="398"
                     /protein_start="35"
                     /score="124.5"
                     /tool="antismash"
                     /translation="VDFSSNDFLSLSTSPAYRARFLDILQQAPPLHPFASGGSRLLDGN
                     SAYAEELENFIAAFHNAPSGLLFNSGYDANVGVFSSIPQPGDLIVYDELIHASAHEGMR
                     LSRAGKRIKFPHSSPDGLRAVLQAEITADPRLLQGRRNVFIAFESVYSMDGDVAPIREF
                     VEIVDQLLPYGNGYFLVDEAHATGVFGPRGSGVVQELGLEDRMFVRVHTFGKALASHGA
                     IVLCCADTRDYLINYARSLIYTTALGFPFLASIRAAYELLVEGKTEQLQHKLGQLIAHF
                     RTGLDNLNHKDSSTFEVEHFTNSPIFSLRSSVPRVLASVCQEQGYTVRAIMPPTVPAGK
                     ERVRVCLHAGNTVEEVDGLLETI"
     mRNA            join(<2171..2358,2409..2502,2556..3889,3942..4516,
                     4592..>4764)
                     /gene="biDA"
                     /product="bifunctional dethiobiotin
                     synthetase/adenosylmethionine-8-amino-7-oxononanoate
                     aminotransferase"
     gene            <2171..>4764
                     /gene="biDA"
     CDS             join(2171..2358,2409..2502,2556..3889,3942..4516,
                     4592..4764)
                     /EC_number="6.3.3.3"
                     /EC_number="2.6.1.62"
                     /NRPS_PKS="Domain: Aminotran_3 (315-667). E-value: 5.4e-23.
                     Score: 73.3. Matches aSDomain:
                     nrpspksdomains_biDA_Aminotran_3.1"
                     /codon_start=1
                     /gene="biDA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 63.9; E-value:
                     2.2e-19)"
                     /gene_kind="biosynthetic-additional"
                     /note="BiDA; penultimate step of biotin biosynthesis;
                     antepenultimate step of biotin biosynthesis"
                     /product="bifunctional dethiobiotin
                     synthetase/adenosylmethionine-8-amino-7-oxononanoate
                     aminotransferase"
                     /protein_id="ACR44943.1"
                     /transl_table=1
                     /translation="MAPVGAALWRSLRAHQVYGANTDVGKTIVSTFLCNAVNRLKNQGK
                     SAFLKPVSTGPLDEADDRHLQRHAPNTLTKCLYQFDEPVSPHIAAKTFAIPRDDEILSS
                     VHRTLSDWANDGVGFALVETAGGVHSPGPNGNSQADLYRPLRLPIILVADSRLGGISSS
                     ISAYESLLLRGYDVHSVLLFKDDYYQNHEYLGNYFRGKSIPLVPVPAPPRRPQEQDPDS
                     RARDLEALDKYYSSVTKSTDVVSLLDELVLKNKQRVEYLDEMASRAQKTIWYPFTQHHG
                     MAAKDITPIDSAYDDFFQTYVTADRSAQQGRLQATFDGSASWWTQGLGHGNPGLALSAA
                     YAAGRYGHVMFPGNIHEPALALAESLLKTVDNPRLQKVFYTDNGSTGMEVALKMGLRAA
                     CDRYGWDASKEQINILGLKGSYHGDTIGVMDCSEPSTYNQRVEWYRGRGHWFDFPLVKM
                     SQGVWQVEVPATLQASLGGNQQFSSLDAVFDVESRVRSDAGQRYRKYILETIERLVTQE
                     GKKFGALIMEPIILGAGGMLFCDPLFQRCLADVVRGNPQLFNRGRLTEPQPQTDLSWSG
                     LPVIFDEVFTGLYRLGRKSSASFLGVNPDIAVNAKLLTGGLVPLCTTLASNEIFNAFTS
                     PEKRDALLHGHSYTAHAVGCQVALDSLRTMNNMDEDGSWNDFKNDWKQPHAGDTARVWS
                     VWSHKLLHNLSHAESVDGVFAIGSVLSISLKDAEGAGYTSTAAKGLQTRLAAGGPQFNV
                     HSRVLGNVLYLMSSVTSKQETLRTIEGILREALL"
     aSDomain        join(3219..3889,3942..4326)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_biDA_Aminotran_3.1"
                     /evalue="5.40E-23"
                     /label="biDA_Aminotran_3.1"
                     /locus_tag="biDA"
                     /protein_end="667"
                     /protein_start="315"
                     /score="73.3"
                     /tool="antismash"
                     /translation="TFDGSASWWTQGLGHGNPGLALSAAYAAGRYGHVMFPGNIHEPAL
                     ALAESLLKTVDNPRLQKVFYTDNGSTGMEVALKMGLRAACDRYGWDASKEQINILGLKG
                     SYHGDTIGVMDCSEPSTYNQRVEWYRGRGHWFDFPLVKMSQGVWQVEVPATLQASLGGN
                     QQFSSLDAVFDVESRVRSDAGQRYRKYILETIERLVTQEGKKFGALIMEPIILGAGGML
                     FCDPLFQRCLADVVRGNPQLFNRGRLTEPQPQTDLSWSGLPVIFDEVFTGLYRLGRKSS
                     ASFLGVNPDIAVNAKLLTGGLVPLCTTLASNEIFNAFTSPEKRDALLHGHSYTAHAVGC
                     QVALDSLRTMNN"
     mRNA            join(<5678..5887,5985..>6956)
                     /gene="biB"
                     /product="biotin synthase"
     gene            <5678..>6956
                     /gene="biB"
     CDS             join(5678..5887,5985..6956)
                     /EC_number="2.8.1.6"
                     /codon_start=1
                     /function="catalyzes the conversion of dethiobiotin to
                     biotin"
                     /gene="biB"
                     /note="BiB; dethiobiotin:sulfur sulfurtransferase; ultimate
                     step of the biotin biosynthesis"
                     /product="biotin synthase"
                     /protein_id="ACR44944.1"
                     /transl_table=1
                     /translation="MSVSFTRSFPRAFIRSYGTVQSSPTAASFASRIPPALQEAVAATA
                     PRTNWTRDEVQQIYETPLNQLTYAAAAVHRRFHDPSAIQMCTLMNIKTGGCSEDCSYCA
                     QSSRYSTGLKATKMSPVDDVLEKARIAKANGSTRFCMGAAWRDMRGRKTSLKNVKQMVS
                     GVREMGMEVCVTLGMIDADQAKELKDAGLTAYNHNLDTSREFYPTIITTRSYDERLKTL
                     SHVRDAGINVCSGGILGLGEADSDRIGLIHTVSSLPSHPESFPVNALVPIKGTPLGDRK
                     MISFDKLLRTVATARIVLPATIVRLAAGRISLTEEQQVACFMAGANAVFTGEKMLTTDC
                     NGWDEDRAMFDRWGFYPMRSFEKETNAATPQQHVDSVAHESEKNPAAPAAEAL"
ORIGIN
        1 cagtgccgga atcatggctt catgatttgc ctgacacatg taccgtacat gaaggacttc
       61 tgcgtcaatg gggatgtgat ttgtgggctc tgggtctgat gataatgcta taaccgagcc
      121 ttttgctttt ccatccgctg taaccacgta gcaatggtct caaggaggcc gtcgacctcc
      181 tccaccgtgt ttcccgcgtg caagcatacg cgtacacgtt ctttccctgc tggaacagtt
      241 ggcggcatga ttgcacggac ggtatatccc tgctcctggc atacgctggc gagcacacgg
      301 ggcacactgc tgcgaaggga aaatatgggt gagttcgtaa aatgttcaac ttcaaaggtc
      361 gacgagtcct tgtggttgag attgtccaac cccgtccgga agtgagcaat cagttgtccg
      421 agtttgtgct ggagctggat atttgttagc tttcccatag gcctgtaatg aaacgttgct
      481 tcctcaagcg aaggaccgta cctgctcagt ttttccctca acgagtagtt cataggcagc
      541 acgtattgaa gcaagaaagg gaaatcccag ggcagtggta tagatcaggc tgcgagcgta
      601 attgatcagg tagtctctag tatccgcaca acacaatacg attgctagca ggacagtcaa
      661 tatcgacacc caaagacaac gcgcgagact tacctccatg actagcgagt gctttaccga
      721 acgtatgtac ccggacgaac atacgatcct ccagtcccaa ctcttggact accccagaac
      781 cgcgtggtcc aaagacgccg gtcgcatgcg cctcatccac aaggaagtat ccgttaccgt
      841 aagggagaag ctggtccacg atctctacga actcacgaat aggcgcaaca tcgccgtcca
      901 tactgtagac ggattcaaag gcgatgaaaa cgttccggcg gccctgaaga agtcggggat
      961 ctgcagttat ttctgcttgc agtactgctc tcaggccatc gggagagcta tgagggaact
     1021 tgatacgctt ccctgctcgt gagagccgca tgccctcatg cgcgctggca tggatgagtt
     1081 catcgtatac tatcagatct ccgggttgcg ggatactcga gaaaacgcca acattggcat
     1141 catatccgga attgaacagt aggccactcg gggcattgtg gaacgcggca atgaagttct
     1201 ccagctcttc ggcataggct gaattgccgt ctaggaggcg ggaccctccg ctggcaaacg
     1261 ggtgcaacgg cggcgcttgt tgcaaaatat ccaaaaaccg cgctcgatag gctggcgatg
     1321 tggatagtga caaaaagtcg tttgatgaga aatcaaccga agacgaaggt agaatcgtca
     1381 gttttcgacg gcacagcttg tcttcccttc gacgtaacgc ctcgcgcagt gagtcacgaa
     1441 ggcattttgg agaatcgccc atctggacga ctgtggttcc aaggccaacg cttgccaata
     1501 ggcaattcag atagctggag acaagattca agtaaccgta aattcaaagg aggatctatt
     1561 ttttctctgg tgagattttg ccagtattaa tgctccagct caaatcacgc tctccaatct
     1621 ccacgagtct agaacgtctt gaagaccctg ctgtatggac ctgagacttg gggtagagcg
     1681 atcactgatg aagtagtgcc tgagtctcac cacattaaca catcatacgg agtagcacaa
     1741 ttcaattcgg cgggacttat ttcatctgtt gggatcaacc ggtgggggaa tggatccgac
     1801 gaacgtggct gggccgctgc ccggttgtgc cgctgcagtg actcggtgag caacgcagtt
     1861 ccctaggcaa gacaaaaggc ttaactcggg ggcctcggcc tggttcctgt ctcactgtgc
     1921 ctgttcctgc tagaacgact atgaacgcct aatgcaaagg gctcgagagt cgaaccacga
     1981 tcgggtttct ttccgcggta aagcgctgca gccttctgca accgaatctg tcggtcatca
     2041 ttcctgttcc ggtcgcagac gggtgccgaa aactgcttct aaagttcagt tggtgtttcg
     2101 gctctgtgat gcgttagcct ttcacattag cgactggttt ctgctgtttt gaaagtctag
     2161 attcaccatc atggcacctg ttggtgccgc gctctggcgc tccctccgcg cccaccaagt
     2221 atacggggcg aacactgacg tcggcaagac aattgtatca acatttcttt gcaatgctgt
     2281 taatcgtctg aaaaatcaag gaaagtcggc gtttttgaag ccggtctcga cggggccatt
     2341 ggatgaagcg gatgacaggt aaggccggga tgtcggcgag agagtgttac cgactcacgc
     2401 gaacacaggc acctgcaacg ccatgctccc aacactttga caaaatgcct ctatcaattt
     2461 gatgaacccg tcagcccaca tatcgcagct aagacgttcg ctgtcagaaa cccaaatcta
     2521 cttcgtccaa cataagcaca cactaacctc gacagattcc tcgagatgac gagatccttt
     2581 cctctgtcca caggaccctt tccgactggg ccaacgacgg agtcgggttc gctctggtag
     2641 aaacggccgg tggtgttcac tcgccgggcc ccaatgggaa ctcccaggcc gacctctacc
     2701 gacctcttcg gttgcccatc atcttggtcg ctgactcccg tcttggggga atctcatcct
     2761 ctatatccgc ctacgagtct ctcttgctcc gcggatacga tgtccactcc gtccttctat
     2821 ttaaggatga ctactatcag aatcatgagt acttggggaa ctacttccgg ggcaagagca
     2881 taccgcttgt cccggtgcct gcaccgcctc ggcgtccgca agagcaagac cccgactcgc
     2941 gagctcgcga tctggaagcg ttggacaagt attacagctc agttacaaaa agcacggatg
     3001 tggtctcgct cctagatgag cttgttttga aaaacaagca gcgtgtcgaa tacctcgacg
     3061 agatggcaag ccgagcacag aagacaatct ggtatccatt tacgcagcac cacggcatgg
     3121 cggcaaagga tatcactccc atcgattccg catacgatga tttcttccag acttacgtga
     3181 ctgcggatcg ttcggcacaa caaggcagac tccaagccac gttcgacgga tccgcatcgt
     3241 ggtggacaca gggactcggt cacggaaatc ccggccttgc tctatctgct gcgtatgccg
     3301 caggacgtta cgggcatgta atgttccccg ggaacattca tgagccggct ttggcgttgg
     3361 cggaatcgct cttgaagact gtggataacc ctcgcctaca gaaggtcttc tatacagaca
     3421 atggcagcac aggtatggag gtagctctca agatgggcct ccgcgctgcg tgcgaccggt
     3481 atggctggga tgcaagcaaa gaacagatca acattctcgg cctcaaaggg agctaccatg
     3541 gcgacacgat cggggttatg gactgctcag agccgtcgac ctacaatcaa agagttgagt
     3601 ggtacagagg ccggggtcac tggtttgatt tccctctggt aaagatgtcc caaggggtct
     3661 ggcaggtcga ggtccccgca acactgcaag cgtcgcttgg cgggaaccag cagttctcat
     3721 cactggatgc agtatttgat gtagaaagca gggtccgctc ggatgctgga cagcgctaca
     3781 ggaagtatat actagagacc attgagcgcc tggttactca agaagggaag aagtttggcg
     3841 ccctaattat ggaacctatc attctaggag ccggcggaat gctcttctgg tcagtataga
     3901 tcctttttta caggaaagtt actgacggac gaccgttata gtgatcccct gttccagcga
     3961 tgcctcgccg atgtcgtccg cggtaacccc cagctcttca accgaggccg gttgacagaa
     4021 ccgcagccgc agaccgacct ctcgtggtcc ggtctccccg tcatctttga cgaggttttc
     4081 acgggcctct accgtcttgg ccgtaaatcc tctgcgtcat ttctaggtgt caacccagac
     4141 atcgctgtca acgcaaagct ccttaccgga ggcctggtgc cgctctgcac taccctagcc
     4201 agcaacgaaa tcttcaacgc gtttacaagc ccggagaagc gagatgcgct cttgcatggg
     4261 cacagctaca ccgcgcatgc ggtcgggtgc caagtagccc ttgattccct tcggacaatg
     4321 aacaatatgg acgaggacgg atcctggaac gacttcaaga acgactggaa acaaccccat
     4381 gccggcgaca ccgcccgggt ctggagcgtc tggtcccaca agctgctcca caacctttcc
     4441 cacgccgaat ccgtcgatgg cgtcttcgct attggtagcg ttctcagcat ctctctgaaa
     4501 gatgcagagg gtgcaggtga gttcccgact ctctctacaa caattctctc gccgattcta
     4561 cccgctaaca tgtgacacct ccactcatta ggctatacat ccactgcggc taagggcctt
     4621 cagacaagat tagcggctgg tgggccgcag tttaacgtcc actctcgggt cctcgggaat
     4681 gtgctttact tgatgtcaag cgtgacgtcc aagcaggaaa cactgcggac aattgagggc
     4741 atcctgcgcg aggcgctgct gtaacttaca acctaactag catgtacatg actaagctgt
     4801 actctctgtg taaacaagcg tcgtctcacc ggcaaccgta agaaaaaagt gtggacaccg
     4861 gaccgccgga taggctggtg cgatcgtttg aacccttgta gaatccagct caccgagtca
     4921 ccgggtctcg atatggagcc aagatttact attgatgccc gctctcgctg gctgagtctg
     4981 gatcttacag agacacacta gctcaggttc actgagctcc ggcctcgcag gcccggcgac
     5041 aatctacccc gctattcgcc aggagcaccg acttccgagg ttattctaaa ttggctctta
     5101 gtgagtctcc cgcagatctg cagtttagca ccttgctttt tgctgtcgaa tcagacccaa
     5161 ggggataaga tcaggcactg ggctcgctga ttggtcggac ccgttctcca tctggaaata
     5221 cgcgatccac ttccgtcagt cgttcactcg aaatacgtaa gacctcagga cttgcgttga
     5281 accagcgttg gggtcaacac tcctgcaaag gaagagtccg tttgcagcga tagcaagcga
     5341 atcaagctaa tcaagccaat caagcgatag gaagcgatag caaataattg tagttgaggt
     5401 caggcaattt gctcagaatt attggaccaa tttggaccaa gttgggccaa tttaggccca
     5461 taataaatta caaagttaca agagttcact ctgtagcggt tttccaccga catttcactc
     5521 ctctccaggc caatgacgag ttacccttct tcaggttcct attgtggccc atgctcctct
     5581 cacctgcatt ttcccctccc gccgcatact tttgatcacc cctcgctgtt tcccgcagtg
     5641 tcaccttgat acactcctcc aaccacccgt catcactatg tctgtctcgt ttacgcggtc
     5701 ctttcctagg gccttcataa ggtcatatgg caccgtccag tcgtcgccca cggccgcttc
     5761 ctttgcgagc agaatccccc cggctctcca ggaggctgtt gcagccactg ccccgcgcac
     5821 caattggact cgcgatgaag tccagcagat ttacgagacc ccgttgaatc aattaaccta
     5881 cgctgctgta tgtttccgat ttgaccgctg cttataaatt attctgtaac gcggatattt
     5941 gaatgaattg ttcgttggac cgaatgttga ctcatgctga ataggccgct gtccaccgcc
     6001 gctttcatga cccgtccgca atccaaatgt gcacgttgat gaacatcaag accggtggat
     6061 gcagtgaaga ttgctcctac tgtgcacagt cttctcggta cagcactggc ctcaaggcca
     6121 ccaaaatgag ccccgtcgac gacgtcctcg agaaggcgag gattgccaaa gcgaacggga
     6181 gcacgcgttt ctgtatggga gcggcgtggc gtgatatgcg gggtcgtaag acgagtttga
     6241 agaatgtcaa gcagatggta tctggcgttc gggaaatggg aatggaagtc tgcgtcacac
     6301 taggcatgat tgatgctgat caggctaagg agctgaaaga tgccggcctg acagcctaca
     6361 accacaacct cgatacttcg cgcgaattct accccacaat catcacaacc cgatcgtacg
     6421 acgaacgact aaagaccttg tctcatgtcc gtgatgcggg cattaacgtc tgctctggtg
     6481 gtattctagg tcttggtgag gctgactctg accgcatcgg cctcatccac acggtttcgt
     6541 cacttccctc gcacccggag tcttttcccg tcaacgcctt ggttccaatc aagggtaccc
     6601 cgttgggtga caggaaaatg atctctttcg ataagctcct ccgcactgtc gcgactgcac
     6661 ggatcgtcct tcccgcaacc atcgtccgcc tcgccgccgg ccgcatttct ctcacagagg
     6721 agcagcaggt tgcctgcttc atggctggtg caaacgctgt cttcactgga gagaagatgt
     6781 tgactactga ctgcaacggc tgggacgagg accgcgccat gtttgaccga tggggcttct
     6841 accccatgcg cagctttgag aaagagacta acgctgccac cccccagcag catgttgact
     6901 ctgttgctca cgagtccgag aagaaccccg ctgcgccggc cgcagaagcc ctatgatagg
     6961 gctctaaaac taccccaccc ccccagcctg atacgctttt ctccctgtcc gtgattggta
     7021 gggaagcg
//
