BGC0001241 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001241 (Claviceps purpurea (ergot fungus))

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,indole	1	77,498	High	ergotamine/ergocryptine	terpene

BGC0001241 - Region 1 - NRPS,indole

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Location: 1 - 77,498 nt. (total: 77,498 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
indole: (indsynth or dmat or indole_PTase)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 77,499
Label		BGC0001241

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase oxygenase

cloA: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

easA: NADH:flavin oxidoreductase/NADH oxidase

smcogs: SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase

easD: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

easE: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Ligases: ligase

lpsA1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

lpsA2: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

lpsB: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

lpsC: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001241.4	1.15	terpene	ergotamine, ergocryptine	Claviceps fusiformis
BGC0002232.2	1.15	other (other)	ergotamine	Claviceps purpurea 20.1
BGC0001267.3	0.60	terpene	elymoclavine, Agroclavine I	Claviceps fusiformis
BGC0001573.3	0.47	other (other)	dihydrolysergic acid	Claviceps africana
BGC0001249.5	0.43	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000348.4	0.43	NRPS	ergovaline	Epichloe festucae var. lolii
BGC0001261.3	0.43	NRPS	AM-toxin	Alternaria alternata
BGC0002135.2	0.42	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001240.4	0.39	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001166.5	0.39	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001241.4	0.98	terpene	ergotamine, ergocryptine	Claviceps fusiformis
BGC0002232.2	0.98	other (other)	ergotamine	Claviceps purpurea 20.1
BGC0001249.5	0.74	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.74	NRPS	AM-toxin	Alternaria alternata
BGC0002135.2	0.73	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001240.4	0.71	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0002164.2	0.70	NRPS	peramine	Epichloe festucae
BGC0000307.4	0.69	NRPS	AbT1	Aureobasidium pullulans
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 77498:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (Ala - Phe - Pro - Ala) + (X - Pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

lpsC: X

AMP-binding (238..642): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIFLAGIIGK
Nearest Stachelhaus code(s):: Ph-Gly DIFLWGLLGK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

lpsB: X

AMP-binding (255..653): X

nrpys: D-Lya

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSVGLVMK
Nearest Stachelhaus code(s):: D-Lya DVFSVGLVMK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

lpsA1: Ala - Phe - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (309..706): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DAIFCGGPLK
Nearest Stachelhaus code(s):: Ala DLFFCGGPLK (91% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1396..1803): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DLVGMAAVGK
Nearest Stachelhaus code(s):: Phe DLVGMAAVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2507..2906): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DITLVAGLIK
Nearest Stachelhaus code(s):: Pro DITLVAGLIK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

lpsA2: Ala - X - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (309..706): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DAVFCVGPAK
Nearest Stachelhaus code(s):: Ala DLFFCGGPLK (79% 8Å match); Tyr DAQTIVGIAK (50% 8Å match); 6-clTrp DAAMCGGVAK (41% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1396..1803): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLAGMGAMIK
Nearest Stachelhaus code(s):: Fo-OH-Orn DLWGMGAVNK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2506..2906): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DITLVAGLIK
Nearest Stachelhaus code(s):: Pro DITLVAGLIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor