LOCUS       BGC0001249              6120 bp    DNA     linear   PLN 02-MAY-2013
DEFINITION  Alternaria alternata strain EV-MIL31 nonribosomal peptide synthetase
            6 (NPS6) gene, complete cds.
ACCESSION   BGC0001249
VERSION     BGC0001249
KEYWORDS    .
SOURCE      Alternaria alternata
  ORGANISM  Alternaria alternata
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae;
            Pleosporaceae; Alternaria; Alternaria sect. Alternaria; Alternaria
            alternata complex.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:12:20
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /db_xref="taxon:5599"
                     /mol_type="genomic DNA"
                     /organism="Alternaria alternata"
                     /strain="EV-MIL31"
     protocluster    1..6120
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[663:5994]"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      664..5994
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..6120
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..6120
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001249"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6120
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            <664..>5994
                     /gene="NPS6"
     mRNA            <664..>5994
                     /gene="NPS6"
                     /product="nonribosomal peptide synthetase 6"
     CDS             664..5994
                     /NRPS_PKS="Domain: AMP-binding (61-468). E-value: 8.8e-81.
                     Score: 263.4. Matches aSDomain:
                     nrpspksdomains_NPS6_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (605-670). E-value: 3.6e-15. Score:
                     47.8. Matches aSDomain: nrpspksdomains_NPS6_PCP.1"
                     /NRPS_PKS="Domain: Condensation_DCL (712-983). E-value:
                     2.6e-47. Score: 153.2. Matches aSDomain:
                     nrpspksdomains_NPS6_Condensation_DCL.1"
                     /NRPS_PKS="Domain: PCP (1170-1240). E-value: 1.3e-17.
                     Score: 55.7. Matches aSDomain: nrpspksdomains_NPS6_PCP.2"
                     /NRPS_PKS="Domain: PCP (1241-1311). E-value: 1.1e-16.
                     Score: 52.7. Matches aSDomain: nrpspksdomains_NPS6_PCP.3"
                     /NRPS_PKS="Domain: Condensation_DCL (1377-1626). E-value:
                     5.1e-26. Score: 83.3. Matches aSDomain:
                     nrpspksdomains_NPS6_Condensation_DCL.2"
                     /codon_start=1
                     /gene="NPS6"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     256.4; E-value: 6.9e-78)"
                     /gene_kind="biosynthetic"
                     /note="fungal virulence determinant; iron uptake protein"
                     /product="nonribosomal peptide synthetase 6"
                     /protein_id="AFN69082.1"
                     /sec_met_domain="AMP-binding (E-value: 2.4e-91, bitscore:
                     292.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.6e-42, bitscore:
                     130.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.6e-18, bitscore:
                     51.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRHQAVSELHFFVSILDTLFSDPDCLLSAFGNVSEEELDELWSW
                     NSPLQPELRFCMHEKVSEQAALHPEKIAIDAWDGTLTYRQVEDYATDLAQTLRLLDDSP
                     NHIIPVLFEKSRWTSVAVLAVMKAGACFALLDPAQPEGRLRAVVQQVNAKLFVSSKAQA
                     TLAARVAPAATIIPISASKFDKVYSPCAAQQPKTTLPPVSPDQPLYIQFTSGSTGLPKG
                     CILTHSQYTSGAIPRADAVGYRSHSRVLDFASYAFDVCIDSMLCTLAHGATLCTPSDER
                     RMNDMSGAMRDMRVTFAGMTPSVARTLDVDILDHLESIALGGEGVSTSDAMSWGQRTRV
                     VNAYGPSEATVGATINDNVAAKPYITMGKRKGCAIWLTEPEDHNKLVPPGAVGELLIEG
                     PIVGNGYLNNPAKTKEVFIEDPEFLIKGSKSYPGRHGRIYKTGDLVRFDPDGNGEPIFV
                     GRQDQQVKLRGQRIELAEIEFNMQKHLPADTQLAVEVIKPSGGGEQTLVAFLVEQKKNG
                     MRHLDGNVFGSFTTKFQAALKAMTKQLAIDLPGYMVPSAYIPLWKMPLLVSCKTDRKRL
                     REIGTSVTRQDLRRFNSVISEKKEPTTEMELKLRSLWGKLLGGEEDFSANDNFFSMGGD
                     SLRAMRLVAAAREAGLILNVPDIMLNPTLSAMAAKAKPLSEEASNDVRPFSMVGEDWDE
                     QSAKTETARLCGVADDEVEDVYPCTPLQEGLMALSAKFQDAYVAQRIATLPSATAQRLK
                     EAFDTAVEGSPILRTRIVNVAGRGLFQVVLRNGQLLREHGADPAEYLRRDREEAMDLGT
                     ALFRYGLVQEASSDKTHFVITMHHAVYDGWSMPLVFDRINRAFNGLQTERSVSFKHFIK
                     HLTSLDPAEAQDYWKERLSGVSPHQFPPLPQKGYITQADSLLEHYVTVPTTAHNKLTLA
                     TIIRGAWALVSSLYMGHPDVVFGETLTGRSAPVNGIEEIEGPMITTVPIRVRLSLDRPI
                     SEYLQSVHAQTVKQIPHEHLGLQNIRRLSKDARIACDLRTGLVLHPREDDVGEVDMETV
                     PANTFLPADDAEAAREALKFNTYALMLVCTLDENGFLIMASFDSKCISKGAMERVLVVM
                     NRVVTAFLGNPESKLGDVAVLDPAEAQDAEAMRPRDIMSDSGIGTSPVDSPKLDAATKA
                     LSPNEEKLRSILGRILGMPETEIKPSDSFFELGGDSIGAMRLVSDARAQGLTLTVAQVF
                     QSQSLAELAASIGNEKEDKLLDILSRILGMSKSEINGSDSFFELGGDSIGAMRLVSDAR
                     AQGLNITVAQVFQSQSLSELASSAEETSSSQTETKSSAPYAALGKEASLYSPDRIGTYL
                     QDQTWEIADIYPTRPLQQLAVEGTVDLPRYSLRYELIKFAAPVNREKLEQACQELVARN
                     EVLRTVFVKDEDKVLGVVLSSLRVPYTEIAVPDGEDINAFAQANIQKDIEAPKPHGSSF
                     VSFMLFTDAKSGASTLVFRISHAQYDEICLPILYEQLSALYADTEVPETIPFSRHVNHV
                     VLDNMPKAIPYWQNLLSGSQMSIMKPDIPLTHRGPADVYMEFDISARPANITIGSLPTA
                     AWALVLARRLSLKDVVFGEVVSGRNIGASNADRIFGPTWQYIPFRVPFNNSWTYLDLLN
                     FVQHQHITSAAYESMGFTEIVEKCTDWDAKKVNWFDTVVHQAPAWVEEMPFGNGVEAKF
                     ETTYPHGEPLREWKCQAFVKDGGKKLGIEIVTFAEWIDVAKGVLKEVGEALECLTKSRA
                     TEKIFEGQDSGEQSLADFEV"
     aSDomain        847..2067
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NPS6_AMP-binding.1"
                     /evalue="8.80E-81"
                     /label="NPS6_AMP-binding.1"
                     /locus_tag="NPS6"
                     /protein_end="468"
                     /protein_start="61"
                     /score="263.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="SEQAALHPEKIAIDAWDGTLTYRQVEDYATDLAQTLRLLDDSPNH
                     IIPVLFEKSRWTSVAVLAVMKAGACFALLDPAQPEGRLRAVVQQVNAKLFVSSKAQATL
                     AARVAPAATIIPISASKFDKVYSPCAAQQPKTTLPPVSPDQPLYIQFTSGSTGLPKGCI
                     LTHSQYTSGAIPRADAVGYRSHSRVLDFASYAFDVCIDSMLCTLAHGATLCTPSDERRM
                     NDMSGAMRDMRVTFAGMTPSVARTLDVDILDHLESIALGGEGVSTSDAMSWGQRTRVVN
                     AYGPSEATVGATINDNVAAKPYITMGKRKGCAIWLTEPEDHNKLVPPGAVGELLIEGPI
                     VGNGYLNNPAKTKEVFIEDPEFLIKGSKSYPGRHGRIYKTGDLVRFDPDGNGEPIFVGR
                     QDQQVKLR"
     aSModule        847..2673
                     /complete
                     /domains="nrpspksdomains_NPS6_AMP-binding.1"
                     /domains="nrpspksdomains_NPS6_PCP.1"
                     /locus_tags="NPS6"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1288..1344
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0001"
                     /evalue="5.20E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="NPS6"
                     /protein_end="227"
                     /protein_start="208"
                     /score="22.1"
                     /tool="antismash"
                     /translation="YIQFTSGSTGLPKGCILTH"
     CDS_motif       1825..1914
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0002"
                     /evalue="4.10E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="NPS6"
                     /protein_end="417"
                     /protein_start="387"
                     /score="44.7"
                     /tool="antismash"
                     /translation="PPGAVGELLIEGPIVGNGYLNNPAKTKEVF"
     CDS_motif       2032..2097
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0003"
                     /evalue="5.00E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="NPS6"
                     /protein_end="478"
                     /protein_start="456"
                     /score="34.6"
                     /tool="antismash"
                     /translation="FVGRQDQQVKLRGQRIELAEIE"
     aSDomain        2479..2673
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NPS6_PCP.1"
                     /evalue="3.60E-15"
                     /label="NPS6_PCP.1"
                     /locus_tag="NPS6"
                     /protein_end="670"
                     /protein_start="605"
                     /score="47.8"
                     /tool="antismash"
                     /translation="ELKLRSLWGKLLGGEEDFSANDNFFSMGGDSLRAMRLVAAAREAG
                     LILNVPDIMLNPTLSAMAAK"
     CDS_motif       2545..2574
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0004"
                     /evalue="4.00E-01"
                     /label="PCP_mE"
                     /locus_tag="NPS6"
                     /protein_end="637"
                     /protein_start="627"
                     /score="4.4"
                     /tool="antismash"
                     /translation="NFFSMGGDSL"
     aSDomain        2800..3612
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NPS6_Condensation_DCL.1"
                     /domain_subtypes="Condensation_DCL"
                     /evalue="2.60E-47"
                     /label="NPS6_Condensation_DCL.1"
                     /locus_tag="NPS6"
                     /protein_end="983"
                     /protein_start="712"
                     /score="153.2"
                     /tool="antismash"
                     /translation="EDVYPCTPLQEGLMALSAKFQDAYVAQRIATLPSATAQRLKEAFD
                     TAVEGSPILRTRIVNVAGRGLFQVVLRNGQLLREHGADPAEYLRRDREEAMDLGTALFR
                     YGLVQEASSDKTHFVITMHHAVYDGWSMPLVFDRINRAFNGLQTERSVSFKHFIKHLTS
                     LDPAEAQDYWKERLSGVSPHQFPPLPQKGYITQADSLLEHYVTVPTTAHNKLTLATIIR
                     GAWALVSSLYMGHPDVVFGETLTGRSAPVNGIEEIEGPMITTVPIRVRL"
     aSModule        2800..4383
                     /domains="nrpspksdomains_NPS6_Condensation_DCL.1"
                     /domains="nrpspksdomains_NPS6_PCP.2"
                     /incomplete
                     /locus_tags="NPS6"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       2866..2976
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0005"
                     /evalue="5.30E-02"
                     /label="C2_DCL_024-062"
                     /locus_tag="NPS6"
                     /protein_end="771"
                     /protein_start="734"
                     /score="5.9"
                     /tool="antismash"
                     /translation="AYVAQRIATLPSATAQRLKEAFDTAVEGSPILRTRIV"
     CDS_motif       3163..3213
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0006"
                     /evalue="1.40E-07"
                     /label="C3_DCL_135-156"
                     /locus_tag="NPS6"
                     /protein_end="850"
                     /protein_start="833"
                     /score="24.1"
                     /tool="antismash"
                     /translation="MHHAVYDGWSMPLVFDR"
     CDS_motif       3508..3603
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0007"
                     /evalue="1.50E-10"
                     /label="C5_DCL_263-294"
                     /locus_tag="NPS6"
                     /protein_end="980"
                     /protein_start="948"
                     /score="33.5"
                     /tool="antismash"
                     /translation="DVVFGETLTGRSAPVNGIEEIEGPMITTVPIR"
     CDS_motif       3652..3717
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0008"
                     /evalue="1.00E-03"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="NPS6"
                     /protein_end="1018"
                     /protein_start="996"
                     /score="11.4"
                     /tool="antismash"
                     /translation="HAQTVKQIPHEHLGLQNIRRLS"
     aSDomain        4174..4383
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NPS6_PCP.2"
                     /evalue="1.30E-17"
                     /label="NPS6_PCP.2"
                     /locus_tag="NPS6"
                     /protein_end="1240"
                     /protein_start="1170"
                     /score="55.7"
                     /tool="antismash"
                     /translation="EEKLRSILGRILGMPETEIKPSDSFFELGGDSIGAMRLVSDARAQ
                     GLTLTVAQVFQSQSLAELAASIGNE"
     CDS_motif       4243..4272
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0009"
                     /evalue="9.30E-02"
                     /label="PCP_mE"
                     /locus_tag="NPS6"
                     /protein_end="1203"
                     /protein_start="1193"
                     /score="6.0"
                     /tool="antismash"
                     /translation="SFFELGGDSI"
     aSDomain        4387..4596
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NPS6_PCP.3"
                     /evalue="1.10E-16"
                     /label="NPS6_PCP.3"
                     /locus_tag="NPS6"
                     /protein_end="1311"
                     /protein_start="1241"
                     /score="52.7"
                     /tool="antismash"
                     /translation="EDKLLDILSRILGMSKSEINGSDSFFELGGDSIGAMRLVSDARAQ
                     GLNITVAQVFQSQSLSELASSAEET"
     CDS_motif       4456..4485
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0010"
                     /evalue="9.30E-02"
                     /label="PCP_mE"
                     /locus_tag="NPS6"
                     /protein_end="1274"
                     /protein_start="1264"
                     /score="6.0"
                     /tool="antismash"
                     /translation="SFFELGGDSI"
     aSDomain        4795..5541
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NPS6_Condensation_DCL.2"
                     /domain_subtypes="Condensation_DCL"
                     /evalue="5.10E-26"
                     /label="NPS6_Condensation_DCL.2"
                     /locus_tag="NPS6"
                     /protein_end="1626"
                     /protein_start="1377"
                     /score="83.3"
                     /tool="antismash"
                     /translation="ELIKFAAPVNREKLEQACQELVARNEVLRTVFVKDEDKVLGVVLS
                     SLRVPYTEIAVPDGEDINAFAQANIQKDIEAPKPHGSSFVSFMLFTDAKSGASTLVFRI
                     SHAQYDEICLPILYEQLSALYADTEVPETIPFSRHVNHVVLDNMPKAIPYWQNLLSGSQ
                     MSIMKPDIPLTHRGPADVYMEFDISARPANITIGSLPTAAWALVLARRLSLKDVVFGEV
                     VSGRNIGASNADRIFGPTWQYIPFRVP"
     CDS_motif       4807..4893
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0011"
                     /evalue="1.30E-09"
                     /label="C2_LCL_024-062"
                     /locus_tag="NPS6"
                     /protein_end="1410"
                     /protein_start="1381"
                     /score="30.4"
                     /tool="antismash"
                     /translation="FAAPVNREKLEQACQELVARNEVLRTVFV"
     CDS_motif       5119..5169
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0012"
                     /evalue="3.20E-01"
                     /label="C3_LCL_132-143"
                     /locus_tag="NPS6"
                     /protein_end="1502"
                     /protein_start="1485"
                     /score="3.9"
                     /tool="antismash"
                     /translation="YDEICLPILYEQLSALY"
     CDS_motif       5440..5493
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NPS6_0013"
                     /evalue="1.60E-04"
                     /label="C5_DCL_263-294"
                     /locus_tag="NPS6"
                     /protein_end="1610"
                     /protein_start="1592"
                     /score="14.2"
                     /tool="antismash"
                     /translation="DVVFGEVVSGRNIGASNA"
ORIGIN
        1 ggatggatga gttgttcctt acaattgatc accgcctctc cttagaaaaa ccagatatta
       61 actttgaaat gaacagacca cacagcagct tgaacggcgg caagatgcac ctacgtaacg
      121 gcgagcatgc atcgcacttt tagcgggcgc tccagacgag catatcgcaa agaaagtgca
      181 aagcaattgc gcatcaacca agaatggcaa tcatgaagcc ctaaacacga acggccacag
      241 ctccaagaca aacgaggctc gagcaaaaat cccaaataat ctaacaccgg atgctttact
      301 ggacgccgtt cttgctgctt gggcggcact catgcacaga taccaaagag atgtcttcca
      361 tcaattctct tggggcgtgg tcaatgccaa cggtcgagcg gcacaaacga tcaacacagc
      421 tgatctcaac ttgacaagcc acaagacggc cgcgagtttg aggtcacaga tcagcaatgt
      481 gcgattgaag gagtacgatg catataatgc cactctcttt ttgaacgatg gttcgacaca
      541 cgaggtatta gttcactcat ccatctttac gacaagactg acctgaatca gtggactttt
      601 gaggtatctc tcaacaggga aggagactct ctccatgcta cctcaagatg gcagacgaat
      661 accatgtctc gccatcaagc cgtctctgaa cttcatttct ttgtttcaat tcttgatacc
      721 ctatttagcg accccgattg cctgctgtcc gcattcggca atgtttccga agaagagctc
      781 gatgagttgt ggagctggaa tagtcctcta cagccagagt tgagattctg catgcatgag
      841 aaggtctccg agcaagctgc gctacatccg gaaaagatcg ccatagatgc atgggacggc
      901 actttgacat atcgtcaagt ggaagactac gctaccgatc tggctcaaac tttacgacta
      961 ctggacgatt cgccgaacca tatcattccg gtgcttttcg agaaatcacg atggacttcg
     1021 gtggcggtgc tcgcagttat gaaggccgga gcatgcttcg cgctgcttga tccagcacaa
     1081 cctgaaggcc gactacgagc agtagtacag caagtcaacg ctaagctctt tgtttcatcc
     1141 aaagctcaag ctacgctagc tgcacgtgtc gctcccgcgg caacaatcat tccaatctca
     1201 gcatccaagt tcgacaaggt gtactctcca tgcgctgcgc agcaaccaaa gacaactctc
     1261 ccgccagtct caccagatca gcccctgtac atccagttca ctagtggcag tactggtctt
     1321 ccaaaaggat gcattcttac gcacagtcag tatacgtctg gtgcgatacc acgagctgat
     1381 gctgtgggct atcgatcgca ttctcgcgtg ctggactttg cgagttatgc ctttgacgta
     1441 tgcattgact ctatgttgtg tacgctcgct cacggcgcga cgctctgtac accttccgat
     1501 gagcgccgta tgaacgacat gagtggcgcg atgcgcgata tgcgcgtgac cttcgctggc
     1561 atgacccctt ctgttgctcg gacgctggat gtagacatcc tagatcatct agaatctatc
     1621 gctctaggtg gtgaaggggt gtcaactagc gatgccatgt cgtggggtca aaggacaagg
     1681 gtcgttaacg cctatggacc atctgaagct acagttggcg cgaccatcaa tgataatgtg
     1741 gctgccaaac catacattac catgggtaag cgcaagggtt gtgcgatctg gcttactgag
     1801 cctgaagatc acaacaagct tgtacctcct ggtgcagttg gcgaactgct cattgagggc
     1861 cctatagttg gtaatggtta tctcaacaac ccggcgaaga ccaaggaagt tttcattgaa
     1921 gatcctgaat tcttgattaa aggaagtaag agctacccag gtcgacatgg acgcatctac
     1981 aagactggtg atctagtacg tttcgatccc gacggcaacg gcgagcccat ctttgtcgga
     2041 cggcaggacc agcaagtcaa gctccgcggc caacgtatcg agctggccga aatcgagttc
     2101 aatatgcaga agcatcttcc ggcagatact cagctcgctg ttgaagtcat caagcccagc
     2161 ggcggtggtg agcagacgct agtcgctttc ctcgtggaac agaaaaagaa cggcatgcga
     2221 catttggatg gaaacgtctt cggcagcttc accaccaagt tccaagcagc tctcaaagct
     2281 atgactaaac agctcgctat tgaccttcct ggatacatgg taccgagcgc ttacatcccg
     2341 ctttggaaga tgccgctact tgtatcttgc aagaccgaca gaaagcgtct ccgcgaaatc
     2401 ggcacttcag tcacacgcca ggatctcaga cgcttcaact cggtcatctc ggagaagaag
     2461 gagcctacaa ctgagatgga gctcaagttg cgatcgctat ggggaaagct gcttggtggt
     2521 gaggaagact tcagtgccaa tgacaacttc tttagcatgg gtggagattc actaagggct
     2581 atgaggctgg tagctgcggc aagagaggct ggactcatat tgaacgtccc ggatatcatg
     2641 ctgaatccaa cactgtctgc catggctgcc aaggcgaagc ctttgtcgga ggaagcatcg
     2701 aatgatgtac gacctttctc tatggtcggc gaagattggg atgaacagtc ggccaagacc
     2761 gagactgcca ggctctgtgg tgttgctgat gacgaagttg aggatgttta cccttgcaca
     2821 ccgctacaag aaggacttat ggctctctca gccaagtttc aggatgcata tgtcgctcag
     2881 cgaatcgcaa cactaccgtc ggcgaccgct cagcgtctga aggaagcttt tgataccgcc
     2941 gtagagggat cgccaatcct acgtaccaga atcgtcaacg tggctggtcg gggcttgttc
     3001 caggtcgtcc tcagaaacgg ccagctgttg cgcgagcatg gcgctgaccc cgcagaatat
     3061 cttcgacgcg accgcgaaga agccatggat ctgggaaccg ctctgttccg ctatggcttg
     3121 gttcaagaag ctagcagcga caagacccac tttgtcatca cgatgcacca tgctgtttac
     3181 gacggttggt caatgcctct ggtctttgat cgcatcaacc gtgcttttaa tggtcttcag
     3241 actgaaaggt ctgtatcttt caagcacttt attaagcacc tcactagcct ggatccagcg
     3301 gaggctcaag attattggaa ggagcggtta agtggtgtca gcccgcatca gttccctcct
     3361 ctgccgcaga aggggtacat cactcaagcc gattcgttgc tagagcacta cgttaccgtc
     3421 ccaacaacgg cacacaacaa acttactctc gcaactatta tccgtggagc ctgggccctg
     3481 gtatcgtcat tgtatatggg ccatccagat gtcgtcttcg gtgagacact tacaggtcgc
     3541 tccgcgcctg tgaacggaat cgaggagatt gagggtccta tgatcacgac tgttccaatc
     3601 cgcgttcgat tgagtcttga ccggccaatt tctgaatacc tccaatccgt ccacgcgcaa
     3661 accgtgaagc agattcctca cgagcacttg gggttgcaaa acatccgccg cctgagtaag
     3721 gacgcccgta tagcgtgcga cctgcgtact ggtcttgtct tgcatcctag ggaggacgat
     3781 gtcggcgaag tcgatatgga gactgtacca gctaacacgt ttctccctgc tgatgatgcg
     3841 gaagctgcac gcgaagcact caagttcaac acctacgcac tcatgctcgt gtgcacacta
     3901 gatgagaatg gtttcttgat tatggccagt ttcgattcga agtgcattag caagggtgcc
     3961 atggagaggg tgctcgttgt gatgaaccgt gtcgtcacag cgttcttggg taaccctgaa
     4021 agcaagctag gcgacgtcgc tgtacttgat cctgccgaag cgcaagatgc ggaggcgatg
     4081 agaccgcgag atatcatgag tgacagtggc attggtacgt cgccggtcga tagcccgaag
     4141 ctggatgctg cgacgaaggc gttgtcgcca aacgaagaga agctgcgtag catcctcggc
     4201 cgcatcttgg gcatgcctga gacggagatc aagcctagcg acagcttctt cgaactaggt
     4261 ggcgactcga ttggtgccat gcgacttgtt tctgatgcac gcgcccaagg cttgaccctt
     4321 accgtggctc aagttttcca aagccaatct ttggctgagc tggcggcttc aatcggaaac
     4381 gagaaagagg acaagctgtt ggacatcttg agtcgcattc ttggtatgtc caagagcgag
     4441 atcaacggca gtgacagctt cttcgaacta ggaggagatt ccatcggcgc catgcgcctt
     4501 gtttctgatg cacgcgctca aggcctcaac atcaccgtcg cgcaggtctt ccaaagccaa
     4561 tcactttcgg aactcgcctc gtcagccgaa gagacaagct cctcgcagac tgagaccaaa
     4621 tctagcgcac cttacgctgc gcttggcaag gaagcgtcac tgtacagtcc cgaccgcatc
     4681 ggcacgtatc ttcaggatca gacttgggag attgcggata tctatccgac aagacctctt
     4741 cagcagcttg ctgttgaggg tactgttgac ctaccacgct actcactgcg ttacgagctc
     4801 atcaagttcg cggcaccagt caaccgcgaa aagctggagc aagcctgcca agagcttgtt
     4861 gcacgcaacg aagtcttgcg cactgtcttc gtgaaagacg aggacaaggt gcttggtgtg
     4921 gttctgtcct ctctacgagt cccctacaca gagatcgcag tacctgatgg tgaagacatc
     4981 aatgcattcg cccaagcaaa catacaaaag gacatcgaag caccgaagcc acatggttca
     5041 tcctttgtct cgtttatgct cttcactgat gccaagtccg gcgcttcaac ccttgtcttc
     5101 cgcatctccc acgcccaata tgacgaaatc tgcttaccca tcctgtacga gcagctttcg
     5161 gcgctatacg cagataccga ggtaccagag accataccat tcagcagaca cgtaaaccac
     5221 gtcgtgctag acaacatgcc aaaagccatt ccatactggc aaaacctcct ctccggctcc
     5281 caaatgtcta tcatgaagcc tgacattccg ctcacacatc gcggaccggc agatgtatac
     5341 atggaatttg acatctcagc gcgtccagca aacatcacga ttggttcact gccaacagct
     5401 gcatgggcac ttgttctcgc gcgccgtctc agcctcaagg acgtagtctt tggcgaagtc
     5461 gtttcaggca ggaacattgg cgcttcgaac gctgatcgta tctttggacc tacgtggcag
     5521 tacattccct tccgcgtgcc tttcaacaac agctggacat acctcgactt gttaaacttt
     5581 gtgcagcacc agcatattac ttctgcggct tacgaatcca tgggcttcac tgagatcgta
     5641 gagaaatgca cagattggga tgcgaaaaag gtcaactggt ttgacactgt tgttcatcag
     5701 gcgcctgctt gggtcgagga gatgccgttt ggtaatggtg ttgaggcgaa gttcgagaca
     5761 acgtatccac atggtgagcc actgagggag tggaagtgtc aggcttttgt caaggatgga
     5821 ggaaagaagc ttgggattga gattgttact tttgcggagt ggatcgacgt ggccaagggg
     5881 gtgctgaagg aggttggtga ggcgcttgag tgcttgacga agagcagggc gacggagaag
     5941 atattcgaag gtcaggattc gggagagcag agtctggcgg attttgaagt gtaatgtagg
     6001 tcgatcgtat catggtgttt ggtctatagg cgttggagct agggaaatca tatcactttt
     6061 catgacattt ggaacttcga ctgtgtgaga caggtcaatt tacaatgctg cgccataatg
//
