LOCUS       BGC0001254              7794 bp    DNA     linear   PLN 06-FEB-2015
DEFINITION  Alternaria alternata ACTTS3 gene for polyketide synthase, complete
            cds.
ACCESSION   BGC0001254
VERSION     BGC0001254
KEYWORDS    .
SOURCE      Alternaria alternata
  ORGANISM  Alternaria alternata
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae;
            Pleosporaceae; Alternaria; Alternaria sect. Alternaria; Alternaria
            alternata complex.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 00:17:20
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7794
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /collected_by="Peever,T.L. and Timmer,L.W."
                     /country="USA"
                     /db_xref="taxon:5599"
                     /isolate="SH20"
                     /mol_type="genomic DNA"
                     /organism="Alternaria alternata"
                     /strain="Tangerine pathotype"
     protocluster    1..7794
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[82:7456]"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      83..7456
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..7794
                     /SMILES="C([*])C(-O)"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7794
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001254"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7794
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            83..7456
                     /gene="ACTTS3"
     CDS             83..7456
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (7-436). E-value:
                     1.1e-171. Score: 563.2. Matches aSDomain:
                     nrpspksdomains_ACTTS3_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (548-865). E-value: 2.4e-91.
                     Score: 298.0. Matches aSDomain:
                     nrpspksdomains_ACTTS3_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (937-1119). E-value: 5.5e-24.
                     Score: 77.2. Matches aSDomain:
                     nrpspksdomains_ACTTS3_PKS_DH.1"
                     /NRPS_PKS="Domain: cMT (1381-1594). E-value: 1.3e-79.
                     Score: 259.2. Matches aSDomain:
                     nrpspksdomains_ACTTS3_cMT.1"
                     /NRPS_PKS="Domain: PKS_KR (2088-2264). E-value: 1.1e-52.
                     Score: 170.5. Matches aSDomain:
                     nrpspksdomains_ACTTS3_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (2382-2443). E-value: 3.2e-12.
                     Score: 38.5. Matches aSDomain:
                     nrpspksdomains_ACTTS3_PKS_PP.1"
                     /codon_start=1
                     /gene="ACTTS3"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     itr_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 220.6; E-value:
                     6.3e-67)"
                     /gene_kind="biosynthetic"
                     /product="polyketide synthase"
                     /protein_id="BAJ14522.1"
                     /sec_met_domain="PP-binding (E-value: 2.4e-11, bitscore:
                     30.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 4.3e-93, bitscore: 298.0,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="itr_KS (E-value: 4.7e-174, bitscore:
                     565.2, seeds: 96, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 5.3e-46, bitscore:
                     143.0, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MTPLREPIAVIGSACRFPGGANSPHKLWELLRDPRDILREFPDDR
                     LVLSKFYNGNANHHGSTNVRNRSYLLSEDIRAFDAPFFHINPREADGMDPAQRILLEAV
                     YEALEAAGYTMEQMQGTHTSVFVGVMNSDWWDLQMRDTETIATHAATGTARSIVSNRIS
                     YVFDLKGVSMTIDTACSSSLVALHQAVQSLRSGESTAAIVGGANILLDPAMYIAESTLQ
                     MLSPESRSRMWDKSANGYARGEGCAAVFLKPLTRAIADGDHIECVIRETGVSSDGRTQG
                     ITMPSAAAQAALIKSTYRSAGLDPLADRCQYFECHGTGTPAGDPIEAQAIAEAFFSHSG
                     EDAEIYVGSIKTVIGHLEGCAGLAGLLKASLAIQNRTIPANMLFNDLNPLIGPYYRNLK
                     ILQAAKPWPQDIHGPRRASVNSFGFGGTNAHVILESYEPEMQGTHVLQERSFHGPLTFS
                     ACSKSSLLATISNFTSYIKTNPAVDLQNLAWVLQRKRTEFPVKQHFSGSTHARLIESME
                     AYLQNAGSSGLHNTTIDTKLLYPSEIPRVLGIFTGQGAQWATMGKEFIQNSYLFRESID
                     RSEAALVALPDPPSWSLISELFATVETSRLNEAELSQPLCTAIQIAIVDLMFAAGVKLD
                     AVVGHSSGEIAAAYASGIISAADAMAIAYYRGFHAKRSHGTGGKRGGMVAAELSYEAAL
                     QFCEKVEWAGRLVLAASNSPSSITLSGDLDAVQEAHAYFEKENIFSRLLRVDTAYHSHH
                     MIPCAEPYLTSLKACNIQVSQARSDCIWISSVSGDVQSSSEEQGALTGEYWVDNMVKPV
                     LFSQAVQCSIWNSGPFESIVEIGPHFALKGPTTQVMEAVLESSPPYLSFMRRGHGIETF
                     SDGLGRLWSKLGPSSVDLTGYWKACSSSHIKFQMLKGLPAYAWDHDKVYWKEGRISRNH
                     RLRKDVPHELLGRRTADDSDYELRWRNVLRLTEIPWIRGHKFQGQVLFPAAGYVTMALE
                     ASKALVGDRHVRLFELRDICIRKALVLEEDQSSLETVFSVKRLNADFGIHDENMDLLEA
                     EFSCYVCADETVGTLEKTVSGRIIIHLGSGVDVKLPPAAHFCTDLSPVDLDRFYSTVEE
                     LGLSYQGLFKGLDHAERMLNHSHALAVWENHSMGAGSMVHPALLDVMFQAIFVASISPA
                     APSTLWTPYLPVSIDRIIVDPDHIPVYSHPEVRAHIQAYVTKSSASSIVGDIHLLDSNG
                     IHNGIQVEGLSLKSVAEPTEENDRSIFSQTVWDTDIASGTDFLRDRKEDAQESKLIHAI
                     ERVALFHFRSLVEAITVEKAKTLAWHHQLFLKAVKANIETIRTGGNPVVRQEWLCDTRE
                     TIEDLRAQHPGQIDLNLMHAVSEKLISIVCGETQILEVMLQDDMLNDFYMRGRGFETMN
                     NCIARAVQQIAHKHPRAKYLEIGAGTGGTTHRILDTIESAYTSYEYTDISSAFFEKASH
                     KFDKHASKMVFKVLDIEKDVVDQGFENGAYDVVIAANVLHATRTLSGTMGHIRSLLKPG
                     GYLIFMEVTGDQLRLLFLFGALPGWWLGAGEGRSLGPGVSTIAWDNILRNTGFSGVDDV
                     FYDFPDRSRHTCSVMISQAVDDQLRLLRDPLAATGMPISEQVLIIGGDTSSVSQLAYDT
                     KRLISPWASCVAINNIDGLDSRRLPSRFSVICLTELDKPLFSEIMSEQRLSNLQNLFAT
                     ANVVFWITSGCNEGTPVANMMVGIGRALATELPHLTLQFLDVKTVERLKPSIVAQSFFR
                     LVLAKPLVMAEKSMLWTTEPELVFDGDDILIPRVLPDKEMNNRFNAARRPISENLWKES
                     TCIELSNADNSSAPALFEIKNTIRPGETTIDVKYSVCLGKRCTFVLGVVSGTSDTVLAI
                     SDTNASSVRISKEHVFFLPHDFSGNSATLLLDTANHVLAAKLLRCISPNSIALIYEPGV
                     RLAAAIRHHAHENTVDVFPATSNREKCGEGWAFIHPHATERDIRTIIPRNTGCFINLSF
                     KPPGALSRALLQQTIIHGPDCLSQIVSSADGFLLEAAFNWATTGLLSLDSVETVSVQSY
                     VGTTRPSRDFPLVFDWTAPRLPVTVKPLEPKGLFLPDKTYLMIGMTGDLGRSLCRWMAE
                     HGARYVVLTSRNAEVDSAWIESMAAIGATVKVYKMDVSNRKSVLGVYTTIKNSLPTIAG
                     VCNAAMVLEDRLFANMTVGALSKVFEPKVEGSKVLDEIFHELNLDFFILFSSLTSILGN
                     GGQSNYHAANLFMTSMCAQRRARGLAASVMHIGMVADIGYVARSDRHIENHLRKLQYHP
                     MSETDMHYLFAEAVMSSRADHPGNWNIVSGIETFVDAPGVKLRPPHYHNPRFAHYVREE
                     NARKEDLRTDKTEKSVKELLEDAISEEDVTTVFQQAFLIKLERLTQLESHRIDANKSLL
                     NLGVDSLSAVEIRNWFLQTVGVDIPVLKLLRGDTVSEISIDATKKYLAQRTS"
     aSDomain        104..1390
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ACTTS3_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="1.10E-171"
                     /label="ACTTS3_PKS_KS.1"
                     /locus_tag="ACTTS3"
                     /protein_end="436"
                     /protein_start="7"
                     /score="563.2"
                     /tool="antismash"
                     /translation="IAVIGSACRFPGGANSPHKLWELLRDPRDILREFPDDRLVLSKFY
                     NGNANHHGSTNVRNRSYLLSEDIRAFDAPFFHINPREADGMDPAQRILLEAVYEALEAA
                     GYTMEQMQGTHTSVFVGVMNSDWWDLQMRDTETIATHAATGTARSIVSNRISYVFDLKG
                     VSMTIDTACSSSLVALHQAVQSLRSGESTAAIVGGANILLDPAMYIAESTLQMLSPESR
                     SRMWDKSANGYARGEGCAAVFLKPLTRAIADGDHIECVIRETGVSSDGRTQGITMPSAA
                     AQAALIKSTYRSAGLDPLADRCQYFECHGTGTPAGDPIEAQAIAEAFFSHSGEDAEIYV
                     GSIKTVIGHLEGCAGLAGLLKASLAIQNRTIPANMLFNDLNPLIGPYYRNLKILQAAKP
                     WPQDIHGPRRASVNSFGFGGTNAHVILESY"
     aSModule        104..7411
                     /complete
                     /domains="nrpspksdomains_ACTTS3_PKS_KS.1"
                     /domains="nrpspksdomains_ACTTS3_PKS_AT.1"
                     /domains="nrpspksdomains_ACTTS3_PKS_DH.1"
                     /domains="nrpspksdomains_ACTTS3_cMT.1"
                     /domains="nrpspksdomains_ACTTS3_PKS_KR.1"
                     /domains="nrpspksdomains_ACTTS3_PKS_PP.1"
                     /iterative
                     /locus_tags="ACTTS3"
                     /monomer_pairings="pk -> Me-pk"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       590..637
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0001"
                     /evalue="7.90E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="ACTTS3"
                     /protein_end="185"
                     /protein_start="169"
                     /score="18.0"
                     /tool="antismash"
                     /translation="GVSMTIDTACSSSLVA"
     CDS_motif       662..733
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0002"
                     /evalue="9.20E-01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="ACTTS3"
                     /protein_end="217"
                     /protein_start="193"
                     /score="2.7"
                     /tool="antismash"
                     /translation="RSGESTAAIVGGANILLDPAMYIA"
     CDS_motif       779..817
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0003"
                     /evalue="9.90E-04"
                     /label="PKSI-KS_m4"
                     /locus_tag="ACTTS3"
                     /protein_end="245"
                     /protein_start="232"
                     /score="12.3"
                     /tool="antismash"
                     /translation="WDKSANGYARGEG"
     CDS_motif       1016..1063
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0004"
                     /evalue="5.30E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="ACTTS3"
                     /protein_end="327"
                     /protein_start="311"
                     /score="12.3"
                     /tool="antismash"
                     /translation="YFECHGTGTPAGDPIE"
     CDS_motif       1454..1480
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0005"
                     /evalue="9.90E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="ACTTS3"
                     /protein_end="466"
                     /protein_start="457"
                     /score="-4.4"
                     /tool="antismash"
                     /translation="SACSKSSLL"
     CDS_motif       1727..1774
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0006"
                     /evalue="1.80E-04"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="ACTTS3"
                     /protein_end="564"
                     /protein_start="548"
                     /score="14.2"
                     /tool="antismash"
                     /translation="IFTGQGAQWATMGKEF"
     aSDomain        1727..2677
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ACTTS3_PKS_AT.1"
                     /evalue="2.40E-91"
                     /label="ACTTS3_PKS_AT.1"
                     /locus_tag="ACTTS3"
                     /protein_end="865"
                     /protein_start="548"
                     /score="298.0"
                     /specificity="consensus: pk"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="IFTGQGAQWATMGKEFIQNSYLFRESIDRSEAALVALPDPPSWSL
                     ISELFATVETSRLNEAELSQPLCTAIQIAIVDLMFAAGVKLDAVVGHSSGEIAAAYASG
                     IISAADAMAIAYYRGFHAKRSHGTGGKRGGMVAAELSYEAALQFCEKVEWAGRLVLAAS
                     NSPSSITLSGDLDAVQEAHAYFEKENIFSRLLRVDTAYHSHHMIPCAEPYLTSLKACNI
                     QVSQARSDCIWISSVSGDVQSSSEEQGALTGEYWVDNMVKPVLFSQAVQCSIWNSGPFE
                     SIVEIGPHFALKGPTTQVMEAVLESSPPYLSFMRRG"
     CDS_motif       1973..2074
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0007"
                     /evalue="4.20E-10"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="ACTTS3"
                     /protein_end="664"
                     /protein_start="630"
                     /score="32.1"
                     /tool="antismash"
                     /translation="GVKLDAVVGHSSGEIAAAYASGIISAADAMAIAY"
     aSDomain        2894..3439
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ACTTS3_PKS_DH.1"
                     /evalue="5.50E-24"
                     /label="ACTTS3_PKS_DH.1"
                     /locus_tag="ACTTS3"
                     /protein_end="1119"
                     /protein_start="937"
                     /score="77.2"
                     /tool="antismash"
                     /translation="HELLGRRTADDSDYELRWRNVLRLTEIPWIRGHKFQGQVLFPAAG
                     YVTMALEASKALVGDRHVRLFELRDICIRKALVLEEDQSSLETVFSVKRLNADFGIHDE
                     NMDLLEAEFSCYVCADETVGTLEKTVSGRIIIHLGSGVDVKLPPAAHFCTDLSPVDLDR
                     FYSTVEELGLSYQGLFKGL"
     CDS_motif       2978..3052
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0008"
                     /evalue="7.90E-12"
                     /label="PKSI-DH_m2"
                     /locus_tag="ACTTS3"
                     /protein_end="990"
                     /protein_start="965"
                     /score="37.5"
                     /tool="antismash"
                     /translation="WIRGHKFQGQVLFPAAGYVTMALEA"
     aSDomain        4226..4864
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ACTTS3_cMT.1"
                     /domain_subtype="cMT"
                     /evalue="1.30E-79"
                     /label="ACTTS3_cMT.1"
                     /locus_tag="ACTTS3"
                     /protein_end="1594"
                     /protein_start="1381"
                     /score="259.2"
                     /tool="antismash"
                     /translation="MLQDDMLNDFYMRGRGFETMNNCIARAVQQIAHKHPRAKYLEIGA
                     GTGGTTHRILDTIESAYTSYEYTDISSAFFEKASHKFDKHASKMVFKVLDIEKDVVDQG
                     FENGAYDVVIAANVLHATRTLSGTMGHIRSLLKPGGYLIFMEVTGDQLRLLFLFGALPG
                     WWLGAGEGRSLGPGVSTIAWDNILRNTGFSGVDDVFYDFPDRSRHTCSVM"
     aSDomain        6347..6874
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ACTTS3_PKS_KR.1"
                     /evalue="1.10E-52"
                     /label="ACTTS3_PKS_KR.1"
                     /locus_tag="ACTTS3"
                     /protein_end="2264"
                     /protein_start="2088"
                     /score="170.5"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="KTYLMIGMTGDLGRSLCRWMAEHGARYVVLTSRNAEVDSAWIESM
                     AAIGATVKVYKMDVSNRKSVLGVYTTIKNSLPTIAGVCNAAMVLEDRLFANMTVGALSK
                     VFEPKVEGSKVLDEIFHELNLDFFILFSSLTSILGNGGQSNYHAANLFMTSMCAQRRAR
                     GLAASVMHIGMVA"
     CDS_motif       6350..6448
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0009"
                     /evalue="2.80E-17"
                     /label="PKSI-KR_m1"
                     /locus_tag="ACTTS3"
                     /protein_end="2122"
                     /protein_start="2089"
                     /score="55.2"
                     /tool="antismash"
                     /translation="TYLMIGMTGDLGRSLCRWMAEHGARYVVLTSRN"
     CDS_motif       6719..6829
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ACTTS3_0010"
                     /evalue="3.40E-19"
                     /label="PKSI-KR_m4"
                     /locus_tag="ACTTS3"
                     /protein_end="2249"
                     /protein_start="2212"
                     /score="61.4"
                     /tool="antismash"
                     /translation="LDFFILFSSLTSILGNGGQSNYHAANLFMTSMCAQRR"
     aSDomain        7229..7411
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ACTTS3_PKS_PP.1"
                     /evalue="3.20E-12"
                     /label="ACTTS3_PKS_PP.1"
                     /locus_tag="ACTTS3"
                     /protein_end="2443"
                     /protein_start="2382"
                     /score="38.5"
                     /tool="antismash"
                     /translation="FLIKLERLTQLESHRIDANKSLLNLGVDSLSAVEIRNWFLQTVGV
                     DIPVLKLLRGDTVSEI"
ORIGIN
        1 ctgagcaata ataacctcgc ccctggacat tcgatagcac gatacgtttt tcgattcaac
       61 cacgcaatcc cttgactgga caatgacgcc tcttagagaa ccaattgctg ttatcgggtc
      121 cgcatgtcgc tttccaggtg gtgcaaactc gcctcataaa ctctgggagt tgttgagaga
      181 tcctagagac atattgcgtg agtttcctga tgacagattg gtcttgtcga agttctacaa
      241 tggtaatgcg aaccaccacg gcagtacgaa tgtcagaaac cgatcgtacc ttctttccga
      301 agatatacga gcatttgatg cacccttttt ccacatcaac cctcgagaag ccgatggtat
      361 ggatcctgct caacgtattc tcctcgaggc tgtttatgaa gcacttgaag ctgctggata
      421 tacaatggag caaatgcagg gtacccacac gtctgtgttt gttggtgtca tgaattccga
      481 ttggtgggat ctgcagatgc gcgataccga gacgattgcc acccatgcgg caactggaac
      541 agctcgcagc attgtttcta accgtatttc ctacgtgttt gatctcaaag gcgtatccat
      601 gaccattgac acagcttgtt ctagttcctt ggttgcgcta catcaagcag tgcaaagctt
      661 acggagcggt gaatctacag ctgccattgt ggggggtgcc aatattcttc ttgatcctgc
      721 aatgtatatc gccgagtcaa cattacaaat gctgtcacca gaatctcgct cacgtatgtg
      781 ggacaaatcg gcaaatggtt atgcaagagg cgagggatgt gcagcggtct tcctgaagcc
      841 attaacgagg gccatagccg atggtgatca tatcgagtgt gtcatcaggg aaacaggagt
      901 cagttctgat gggcgcacac agggaatcac catgcccagt gctgcagcac aggcagcatt
      961 gatcaaatcg acataccgca gcgctggatt ggacccgttg gcagatcgct gccagtattt
     1021 tgaatgtcat ggaaccggaa ccccagcagg tgaccccatc gaggcccagg ccatcgcaga
     1081 ggcatttttt tcgcattctg gagaggatgc agaaatctac gttgggtcca tcaaaacagt
     1141 gattggacac ctggagggat gtgcaggcct agcaggtctg ctaaaagcct ctctggcaat
     1201 ccaaaatcgc accatacccg ccaatatgct tttcaacgac ttgaatccat tgattgggcc
     1261 atattacaga aacttaaaga tacttcaagc ggcaaaaccg tggccgcagg acatacacgg
     1321 cccccgccga gctagcgtca atagctttgg ttttggtggg acaaacgcgc atgttatcct
     1381 ggagagctat gagcctgaaa tgcagggaac gcatgtttta caggaaaggt ctttccatgg
     1441 cccgttgaca ttctcggcct gctcgaaatc gtctttattg gcaacgatca gcaacttcac
     1501 cagctacatc aaaacgaatc ctgcagttga tctgcaaaac ctagcctggg ttctacaaag
     1561 aaaacgcacc gaatttccgg tcaagcagca tttctcgggc tccacgcacg ctaggttgat
     1621 cgagtccatg gaagcctatc tccagaatgc aggttccagt ggccttcata acacaaccat
     1681 tgacacaaag ctgttgtacc caagtgaaat tcctagggtt cttgggattt tcactggtca
     1741 aggcgcacaa tgggctacta tgggaaaaga gtttattcaa aattcatatc tcttccgaga
     1801 gtcaatcgac cgaagcgagg cagctcttgt agctttgcct gatccccctt cttggtcttt
     1861 gatatctgaa ctcttcgcca cagttgaaac atctcgactc aatgaagcgg aacttagcca
     1921 accactgtgt actgctatac agattgccat cgtggatctt atgttcgcag caggtgttaa
     1981 gctagatgct gtggtgggcc actcttctgg cgaaatcgct gctgcctacg catcagggat
     2041 catttctgca gcggatgcta tggctattgc ctattacagg ggctttcatg ctaaaaggtc
     2101 gcatggaact ggtgggaaga ggggtggaat ggtagctgct gagctttcgt atgaagctgc
     2161 ccttcagttc tgcgagaagg ttgaatgggc tgggcgttta gtgcttgctg ccagcaactc
     2221 tccgtctagc atcacattgt cgggagacct tgacgctgtt caagaagccc atgcatactt
     2281 tgagaaggag aacatatttt ccaggttgtt acgagtcgac actgcttacc actctcacca
     2341 tatgattccc tgcgccgaac cttatctaac atctctgaag gcttgtaaca tccaagtaag
     2401 ccaagctcgg tcggactgca tctggatatc tagcgtatcc ggggatgttc aatcgtcaag
     2461 cgaggagcaa ggagctttga caggagaata ctgggttgac aacatggtga agccagtttt
     2521 gttttcccag gccgtccaat gttcaatttg gaacagcggc cccttcgaat caatcgtaga
     2581 gataggaccg cacttcgcac tgaaggggcc caccacccaa gtcatggaag cggtgcttga
     2641 atcatcaccg ccgtatctga gcttcatgcg ccgtggacat ggaatcgaaa cattttctga
     2701 tggattaggg cgtctatggt ccaagcttgg tccatcttct gttgacttga cggggtactg
     2761 gaaagcttgc agcagtagcc acatcaagtt tcagatgctg aaagggttgc cagcatacgc
     2821 atgggaccat gataaggttt actggaagga aggacgcatc tcccgtaacc accgccttcg
     2881 taaggacgtt ccacatgagc tcttgggtcg ccgtactgca gatgactctg attatgagct
     2941 gcggtggcgc aatgtgctac gattgaccga aatcccttgg atccggggcc acaaattcca
     3001 gggtcaagtt ctgtttcctg ctgctggcta cgtgacaatg gccttggagg cctccaaggc
     3061 tttggtaggg gatcggcacg ttaggctgtt tgagcttcga gatatttgca tccgaaaggc
     3121 tttggtctta gaagaggatc aatctagcct tgagactgtg ttttctgtca aacgactaaa
     3181 tgcagacttt gggatacatg atgagaatat ggacctcctc gaggctgagt tctcttgcta
     3241 tgtttgtgct gacgagactg ttggcactct tgagaagact gtgtcaggta ggatcataat
     3301 tcaccttgga agcggagttg atgtgaagtt gccgccagct gcacacttct gtactgatct
     3361 ctcacctgtt gatctagaca gattttactc cactgtcgaa gaactcggct taagctacca
     3421 aggccttttt aaaggtctag atcatgctga gcggatgctg aaccactccc atgctcttgc
     3481 ggtatgggaa aaccatagca tgggagcagg atctatggtt caccctgcac tattggacgt
     3541 catgtttcag gcgatctttg tagcttccat ttcaccggcc gctcccagta ctctctggac
     3601 accctatctt cccgtctcta tcgatcgcat catcgtggat cctgatcata tacctgtata
     3661 ttctcaccct gaagtgaggg ctcatataca ggcgtacgtt acaaagtcat cggcatcttc
     3721 aattgttggt gacattcacc ttctggattc gaatggcatt cataatggca tccaggttga
     3781 gggattgtct ctaaagtcag tcgccgaacc tactgaagag aacgatcgct cgattttctc
     3841 ccagactgtc tgggatacag acattgccag cggtacagat tttcttagag accgaaagga
     3901 ggatgcgcaa gaaagtaagc tcatccacgc catagagcga gtcgcgttat ttcactttcg
     3961 aagccttgtt gaggcaataa ccgtggagaa agccaaaacc cttgcgtggc accaccagct
     4021 cttcctcaaa gccgtaaaag caaacataga gacaatcaga actggcggga acccagtagt
     4081 tcggcaagaa tggctatgcg atactcgaga aacgattgaa gaccttcgag ctcaacatcc
     4141 aggtcagatt gacctcaacc taatgcacgc agttagtgaa aagctcatat caattgtctg
     4201 tggggaaaca cagattttgg aagtcatgct ccaggatgac atgctcaacg acttttatat
     4261 gcgaggtcgc ggtttcgaga cgatgaataa ctgtattgcc cgtgctgtgc agcagattgc
     4321 acacaagcac ccccgtgcta agtatttgga gatcggtgca gggaccggag gcacgactca
     4381 ccgcatcctg gacaccattg agtcagctta cacttcgtac gagtacaccg atatatcctc
     4441 tgcgtttttc gaaaaagcct cccacaaatt cgacaaacat gcgagtaaga tggtgttcaa
     4501 ggtgctcgat attgagaagg atgtggttga ccaagggttc gagaacggcg cgtatgatgt
     4561 tgtcatcgct gcgaatgttc ttcatgctac acgcacgttg tctggaacaa tgggccatat
     4621 aaggtctcta ttgaaacctg gtggctactt gatcttcatg gaggtcactg gagatcaact
     4681 caggcttttg tttctttttg gcgccctacc cggatggtgg ctcggagctg gggagggtcg
     4741 cagcctaggc cctggagtct cgaccatcgc ttgggacaac atcctccgta acacggggtt
     4801 ttcaggcgta gatgacgtat tctacgattt tcccgatcgc tcgaggcata catgctctgt
     4861 gatgattagt caggcggtcg acgaccagct cagacttctc cgtgatccac tagccgccac
     4921 aggcatgcct attagcgagc aagtcctcat tatcggcgga gacacatcat cggtttccca
     4981 actggcatac gatacaaagc gcctcatttc gccatgggca agttgtgtgg cgattaataa
     5041 cattgatggt ctagactctc gtcggctacc atctagattt tcagtaattt gcttgacaga
     5101 actggacaag ccgttattct cggaaatcat gagcgagcag cgactgagta atttacaaaa
     5161 cctatttgcg accgcaaatg tcgttttctg gattaccagc ggctgtaatg agggaactcc
     5221 ggtcgctaat atgatggtag gaatcggccg cgctcttgcc accgagttac cccatcttac
     5281 gctgcaattc ctggatgtaa aaacagttga aaggctcaaa ccctctattg ttgcacaatc
     5341 attctttcgc ttagttctgg ccaaaccatt agtcatggca gagaaaagta tgctctggac
     5401 aactgagccc gaacttgtgt ttgatggaga tgacatattg atcccacgtg tcctaccaga
     5461 taaagagatg aataataggt tcaatgcggc tcgccgtcct atcagcgaga acctctggaa
     5521 ggagagcacc tgcatcgagc tcagcaacgc cgacaatagc agcgcaccag ctctattcga
     5581 gataaagaac acaataaggc ctggcgaaac gaccatcgat gtcaagtact cagtatgtct
     5641 aggaaagaga tgcaccttcg ttcttggtgt ggtttcgggt acttcagata cggttcttgc
     5701 tatttctgat acgaatgcct cttctgttcg tatctcgaag gagcacgtct tctttctgcc
     5761 ccatgatttc agtggtaatt ccgcaacgct gctgttggac accgcgaatc atgttcttgc
     5821 tgcaaagctt ttgagatgta tctcgcctaa cagtattgct cttatttatg agcccggagt
     5881 ccgacttgct gcagcaatac gacatcatgc gcacgaaaat acagttgatg tcttcccggc
     5941 aacctctaat cgtgaaaaat gtggagaagg ttgggctttt atacatccac acgccacaga
     6001 acgagatata cgtactatta tccctcggaa cactggttgc ttcatcaatc ttagctttaa
     6061 accaccaggt gcactctccc gtgctctcct gcaacaaacc atcatccatg gacctgattg
     6121 tctcagccaa attgtctctt cagccgatgg cttcctccta gaagctgcat ttaactgggc
     6181 aactacgggt ctgttaagct tggatagtgt agagacggtc agtgtccaga gttatgtagg
     6241 tacaaccaga cctagcaggg acttcccgct tgtatttgac tggacagcgc ctcgactgcc
     6301 tgttaccgtc aagcctctgg agcccaaagg cttgttcctt cctgataaga cttatctcat
     6361 gattggtatg acaggagatc tggggcgctc tctctgcaga tggatggccg aacatggtgc
     6421 tcgatatgta gtgctcacca gccgtaatgc tgaggttgac agtgcttgga ttgagagcat
     6481 ggccgcgata ggcgctaccg tcaaggtcta caagatggat gtgtccaatc gaaaatcggt
     6541 ccttggcgtc tacaccacca tcaaaaattc gcttccaacg atagcaggcg tttgcaatgc
     6601 agccatggtg ctcgaggata ggctctttgc taatatgacc gttggtgcct tgagcaaagt
     6661 ttttgaacca aaggttgagg ggtcaaaagt cctggatgag attttccacg aactaaattt
     6721 ggattttttc atactttttt cttcactgac tagtatcctt ggcaatggtg gtcaatccaa
     6781 ttaccacgcc gctaatctat tcatgactag tatgtgcgct caacgccgtg ctcgaggtct
     6841 tgctgcatcg gtcatgcata tcggcatggt tgcggatatt gggtacgttg ccaggagtga
     6901 ccggcatatt gaaaaccacc ttcgtaagct tcaatatcac cccatgtcag agaccgatat
     6961 gcattacctc ttcgctgagg ccgttatgag cagccgtgca gatcatccgg gcaattggaa
     7021 cattgtatct ggcatcgaaa catttgtcga cgcccctggc gtaaagctcc gcccacctca
     7081 ctaccacaac ccgcgcttcg ctcactatgt ccgcgaagaa aatgctagga aggaggattt
     7141 gcgaaccgac aagacagaaa agagtgtaaa agaactcctt gaggatgcaa tttcagaaga
     7201 ggatgttacg acagtatttc aacaggcctt tctgataaag ctggagaggc tgacacagct
     7261 ggagtcccac agaatcgatg ccaataagtc gcttcttaat ctaggtgtgg actctctgtc
     7321 cgccgtagag atccgcaatt ggttcttgca gactgttggt gtggatattc cagttttgaa
     7381 actacttcgc ggcgatacag tctctgaaat cagcattgat gccaccaaaa agtatcttgc
     7441 acaaaggact tcctaagagc gtttacacga tcaagtatta atttctcttc ttacgctgcg
     7501 aatgacccgg tgagccggaa gcagccaatg agccactgcg taaggttgga agatctgcta
     7561 tccttacgct ctcattggtt gttttcggcc taccgggttg ttcgctgcgt agggagggag
     7621 gttatattca caatcagata acgtgaacgg caagcgaacc ggccaggcaa gcgagccgac
     7681 caccccacca agattgcgca cctttgtata taaattagat gacctaacac cacaacgagc
     7741 taccatattg tacaagacat caaactactg ctcagaaaaa aaaaaaaaaa aaaa
//
