LOCUS       BGC0001257              7549 bp    DNA     linear   PLN 15-DEC-1999
DEFINITION  Nodulisporium sp. ATCC74245 polyketide synthase (pks1) gene,
            complete cds.
ACCESSION   BGC0001257
VERSION     BGC0001257
KEYWORDS    .
SOURCE      Nodulisporium sp. ATCC74245
  ORGANISM  Nodulisporium sp. ATCC74245
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Sordariomycetes; Xylariomycetidae; Xylariales; Xylariaceae;
            Nodulisporium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 15:12:47
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7549
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /db_xref="ATCC:74245"
                     /db_xref="taxon:96454"
                     /mol_type="genomic DNA"
                     /organism="Nodulisporium sp. ATCC74245"
                     /strain="MF5954; ATCC 74245"
     subregion       1..7549
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="PKS"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7549
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     regulatory      213..216
                     /gene="pks1"
                     /regulatory_class="CAAT_signal"
     gene            213..7482
                     /gene="pks1"
     regulatory      315..319
                     /gene="pks1"
                     /regulatory_class="TATA_box"
     mRNA            join(360..695,754..1045,1098..2684,2762..7482)
                     /gene="pks1"
                     /product="polyketide synthase"
     CDS             join(400..695,754..1045,1098..2684,2762..7066)
                     /NRPS_PKS="Domain: SAT (8-246). E-value: 2e-81. Score:
                     265.0. Matches aSDomain: nrpspksdomains_pks1_SAT.1"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (385-814). E-value:
                     4.5e-141. Score: 462.4. Matches aSDomain:
                     nrpspksdomains_pks1_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (912-1212). E-value: 8.9e-91.
                     Score: 296.2. Matches aSDomain:
                     nrpspksdomains_pks1_PKS_AT.1"
                     /NRPS_PKS="Domain: PT (1293-1610). E-value: 1.5e-115.
                     Score: 377.8. Matches aSDomain: nrpspksdomains_pks1_PT.1"
                     /NRPS_PKS="Domain: ACP (1659-1726). E-value: 2.9e-14.
                     Score: 45.0. Matches aSDomain: nrpspksdomains_pks1_ACP.1"
                     /NRPS_PKS="Domain: PP-binding (1784-1849). E-value:
                     1.4e-11. Score: 36.7. Matches aSDomain:
                     nrpspksdomains_pks1_PP-binding.1"
                     /NRPS_PKS="Domain: Thioesterase (1914-2148). E-value:
                     1.3e-44. Score: 145.1. Matches aSDomain:
                     nrpspksdomains_pks1_Thioesterase.1"
                     /codon_start=1
                     /function="involved in 1,3,6,8-tetrahydroxynaphthalene,
                     THN, synthesis"
                     /gene="pks1"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     itr_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 75.3; E-value:
                     6.3e-23)"
                     /gene_kind="biosynthetic"
                     /note="PKS"
                     /product="polyketide synthase"
                     /protein_id="AAD38786.1"
                     /sec_met_domain="itr_KS (E-value: 1.8e-159, bitscore:
                     517.1, seeds: 96, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.6e-92, bitscore: 296.2,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2e-13, bitscore:
                     37.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMADQMAFLLFGDQSLDTHGFLADFYRRGNPSVLSKEFLRLTGDA
                     LRDEIDRLPREERRKIPIFRTLQQLNERYHAQQIKFPGIDSALLCIAQLAHYIDRSEKE
                     YEDVTDHRNTKLSGLCTGLFAATAIASSYSLSTLLPIAIQVVLMAFRTGSHVASLAERL
                     SPSTEKSESWTYVLPAAQEAQAKSILAEFHESEGISLAAQAYVSAVSANNIAISGPPAT
                     LQALFSKDLFESRPTAIPVYGPYHAPHLHGLVDIRKMLRLDDEAVTIALAGSKPRSVVM
                     SCVSGEPFRETDTKSLLTAVVHEILNESLIFHKTLSGNANEARGFKGSRILVIPYGPTQ
                     AASTLANVLRSQTKAEVILRPAPQVSRETTASSIGNHGSSGNCKLAIVGMAGRFPDSAS
                     HEALWELLEKGIDAHRVIPADRFPVDTHYDPTGKAINTSHTPYGNWIENPGHFDPRFFN
                     MSPREAFQTDPMQRLALTTAYEALEMSGYVPNRTPSTRLDRIGTFYGQTSDDWREINAA
                     QEVDTYYITGGVRAFGPGRINYHFGFSGPSLNIDTACSSSAAALQVACTSLRAKECDTA
                     IVGGLSCMTNSDIFSGLSRGQFLSKEHNCNTFDNDADGYCRADGCASVIVKRLDDALAD
                     KDNILAVILGTQTNHSADAISITHPHGPTQSILSSSILDEAGVDPLDVDYVEMHGTGTQ
                     AGDGTEMVSVTDVFAPANRHRPADRPLYLGAVKANVGHGEAASGVTALIKVLMMLKKNA
                     IPPHIGIKKEINKTFPKDLGERGVNIAFHKTPFTRKDGKPRKVFVNNFSAAGGNTGLLL
                     EEGPRYKTAVVDPRSQHVITITAKSKAAMIRNAENLVKWMEENPSTSVSHVAYTTTARK
                     IQHYWRMNVVASSLVEAQEAIKERLKSNFVPVSPEQPKVAFLFTGQGSHYAGLGRDLYA
                     HYSIFRQAINEYNQLALIHGFPSFLPLIDGSEPEVTKLSPVVVQLGLASFEMALARLWI
                     SWGIQPSAVLGHSLGEYAALHVAGVLSASDTIYLVGARAKLLVEKCTAGTHAMLAVQGS
                     VETVTEALGERAATTNIACINGPRETVLSGTSTEVAEIAEQLGNAGFKCTQLKVPFAFH
                     SAQVEPILDDFEKLARSVRFYEPKIPVISPLHGKLLEGEPINPAYLRNHARDAVNFLGG
                     LVSAQSSGVIDEKTAWLEVGPHPVCANMVKAAFGATTIAVPTLRRNEATYKTLSATLCT
                     LHSAGLNIDWNEFHRDFDASVRLLDLPAYSFDLKNYWLQYTGEWCLTKNRAALPAPTKA
                     IEPAKPKLSTTTVQKITKEEVKGEIAILETESDLAEENLRKICLGHRCNGTTLTPSTLY
                     ADMAETICEYGYKLLRPETEDIGMNVAHMEVTKTLIFDAKAASQMLRMEVKVNAAENYA
                     DVTWTSGEGARKTEHAVCKVLFGDKQQWLDEFERVNYLIKGRIDALKAAEERGEASKIG
                     RGLAYKLFTALVDYSHQYKGMESVILSSETCEATAKVVFQTSPEDGTFRTPPYWIDSVC
                     HISGFIVNGTDAVDSREQVYISHGWGSMRFAERLSRTKTYQTYIRMQPVKGTKMMAGDA
                     YVFDGDKLIGIGGEIRFQAIPRKVLNVVLPPPGGAAAAARSAPAAKPAAATKAPVKEKK
                     QVTSSNLPAVNKKLSQTSVVSQVMDIISKETGVSHDELADNIAFADLGVDSLMGLTISG
                     RLREDLELNVDSHAFNDHATIGAFKKFLAQFETAPTTVVESSGSSGSSDSDDDIDSDSE
                     VTTPGESDKGSVKGDAPAEAGSNSELQQIVRETICAEMGVEVEEIIAAPDLAALGMDSL
                     MSLSILGILREKTGLNIPSDLLGHNPSLKDIERTLGIEDKPKRAAPAPKPAAPKVSKKT
                     ESKAATKTETTVTKISSAVDAYPHRKATSVLLQGNHRTATRHLFMIPDGSGSATSYTEI
                     SDLGSDVAVWGMFSPFMRTPEEYKCGVYGMATKFIEEMKRRQPEGPYAVSGWSAGGVIA
                     YEIVNQLTKAGDEVSHLLIIDAPCPITIEPLPAGLHAWFAEIGLLGEGDGEAKKIPSWL
                     LPHFAASVTALSNYTADPIPKDKCPKVTTIWCEDGVCKLPTDPRPDPYPTGHALFLLDN
                     RTDFGPNRWDEYLDIEKMTFHHMPGNHFSMMHGDLAKQLGGFLKEGIHA"
     aSDomain        join(424..695,754..1045,1098..1247)
                     /aSDomain="SAT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_SAT.1"
                     /evalue="2.00E-81"
                     /label="pks1_SAT.1"
                     /locus_tag="pks1"
                     /protein_end="246"
                     /protein_start="8"
                     /score="265.0"
                     /tool="antismash"
                     /translation="LLFGDQSLDTHGFLADFYRRGNPSVLSKEFLRLTGDALRDEIDRL
                     PREERRKIPIFRTLQQLNERYHAQQIKFPGIDSALLCIAQLAHYIDRSEKEYEDVTDHR
                     NTKLSGLCTGLFAATAIASSYSLSTLLPIAIQVVLMAFRTGSHVASLAERLSPSTEKSE
                     SWTYVLPAAQEAQAKSILAEFHESEGISLAAQAYVSAVSANNIAISGPPATLQALFSKD
                     LFESRPTAIPVYGPYH"
     aSDomain        join(1665..2684,2762..3028)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="4.50E-141"
                     /label="pks1_PKS_KS.1"
                     /locus_tag="pks1"
                     /protein_end="814"
                     /protein_start="385"
                     /score="462.4"
                     /tool="antismash"
                     /translation="AIVGMAGRFPDSASHEALWELLEKGIDAHRVIPADRFPVDTHYDP
                     TGKAINTSHTPYGNWIENPGHFDPRFFNMSPREAFQTDPMQRLALTTAYEALEMSGYVP
                     NRTPSTRLDRIGTFYGQTSDDWREINAAQEVDTYYITGGVRAFGPGRINYHFGFSGPSL
                     NIDTACSSSAAALQVACTSLRAKECDTAIVGGLSCMTNSDIFSGLSRGQFLSKEHNCNT
                     FDNDADGYCRADGCASVIVKRLDDALADKDNILAVILGTQTNHSADAISITHPHGPTQS
                     ILSSSILDEAGVDPLDVDYVEMHGTGTQAGDGTEMVSVTDVFAPANRHRPADRPLYLGA
                     VKANVGHGEAASGVTALIKVLMMLKKNAIPPHIGIKKEINKTFPKDLGERGVNIAFHKT
                     PFTRKDGKPRKVFVNNFSAAGGNTGLLLEE"
     aSModule        1665..5764
                     /complete
                     /domains="nrpspksdomains_pks1_PKS_KS.1"
                     /domains="nrpspksdomains_pks1_PKS_AT.1"
                     /domains="nrpspksdomains_pks1_PT.1"
                     /domains="nrpspksdomains_pks1_ACP.1"
                     /iterative
                     /locus_tags="pks1"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       2142..2189
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0001"
                     /evalue="1.90E-05"
                     /label="PKSI-KS_m3"
                     /locus_tag="pks1"
                     /protein_end="560"
                     /protein_start="544"
                     /score="16.8"
                     /tool="antismash"
                     /translation="GPSLNIDTACSSSAAA"
     CDS_motif       2331..2369
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0002"
                     /evalue="6.10E-04"
                     /label="PKSI-KS_m4"
                     /locus_tag="pks1"
                     /protein_end="620"
                     /protein_start="607"
                     /score="12.9"
                     /tool="antismash"
                     /translation="FDNDADGYCRADG"
     CDS_motif       2562..2609
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0003"
                     /evalue="2.10E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="pks1"
                     /protein_end="700"
                     /protein_start="684"
                     /score="10.7"
                     /tool="antismash"
                     /translation="YVEMHGTGTQAGDGTE"
     CDS_motif       join(2679..2684,2762..2800)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0004"
                     /evalue="1.10E-02"
                     /label="PKSI-KS_m6"
                     /locus_tag="pks1"
                     /protein_end="738"
                     /protein_start="723"
                     /score="8.7"
                     /tool="antismash"
                     /translation="GAVKANVGHGEAASG"
     aSDomain        3323..4222
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_PKS_AT.1"
                     /evalue="8.90E-91"
                     /label="pks1_PKS_AT.1"
                     /locus_tag="pks1"
                     /protein_end="1212"
                     /protein_start="912"
                     /score="296.2"
                     /tool="antismash"
                     /translation="LFTGQGSHYAGLGRDLYAHYSIFRQAINEYNQLALIHGFPSFLPL
                     IDGSEPEVTKLSPVVVQLGLASFEMALARLWISWGIQPSAVLGHSLGEYAALHVAGVLS
                     ASDTIYLVGARAKLLVEKCTAGTHAMLAVQGSVETVTEALGERAATTNIACINGPRETV
                     LSGTSTEVAEIAEQLGNAGFKCTQLKVPFAFHSAQVEPILDDFEKLARSVRFYEPKIPV
                     ISPLHGKLLEGEPINPAYLRNHARDAVNFLGGLVSAQSSGVIDEKTAWLEVGPHPVCAN
                     MVKAAFGATTIAVPTLRRN"
     CDS_motif       3326..3373
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0005"
                     /evalue="1.20E-04"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="pks1"
                     /protein_end="929"
                     /protein_start="913"
                     /score="14.8"
                     /tool="antismash"
                     /translation="FTGQGSHYAGLGRDLY"
     CDS_motif       3560..3676
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0006"
                     /evalue="8.80E-17"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="pks1"
                     /protein_end="1030"
                     /protein_start="991"
                     /score="53.2"
                     /tool="antismash"
                     /translation="GIQPSAVLGHSLGEYAALHVAGVLSASDTIYLVGARAKL"
     aSDomain        4466..5416
                     /aSDomain="PT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_PT.1"
                     /evalue="1.50E-115"
                     /label="pks1_PT.1"
                     /locus_tag="pks1"
                     /protein_end="1610"
                     /protein_start="1293"
                     /score="377.8"
                     /tool="antismash"
                     /translation="TTTVQKITKEEVKGEIAILETESDLAEENLRKICLGHRCNGTTLT
                     PSTLYADMAETICEYGYKLLRPETEDIGMNVAHMEVTKTLIFDAKAASQMLRMEVKVNA
                     AENYADVTWTSGEGARKTEHAVCKVLFGDKQQWLDEFERVNYLIKGRIDALKAAEERGE
                     ASKIGRGLAYKLFTALVDYSHQYKGMESVILSSETCEATAKVVFQTSPEDGTFRTPPYW
                     IDSVCHISGFIVNGTDAVDSREQVYISHGWGSMRFAERLSRTKTYQTYIRMQPVKGTKM
                     MAGDAYVFDGDKLIGIGGEIRFQAIPRKVLNVVLPP"
     aSDomain        5564..5764
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_ACP.1"
                     /evalue="2.90E-14"
                     /label="pks1_ACP.1"
                     /locus_tag="pks1"
                     /protein_end="1726"
                     /protein_start="1659"
                     /score="45.0"
                     /tool="antismash"
                     /translation="QVMDIISKETGVSHDELADNIAFADLGVDSLMGLTISGRLREDLE
                     LNVDSHAFNDHATIGAFKKFLA"
     aSDomain        5939..6133
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_PP-binding.1"
                     /evalue="1.40E-11"
                     /label="pks1_PP-binding.1"
                     /locus_tag="pks1"
                     /protein_end="1849"
                     /protein_start="1784"
                     /score="36.7"
                     /tool="antismash"
                     /translation="IVRETICAEMGVEVEEIIAAPDLAALGMDSLMSLSILGILREKTG
                     LNIPSDLLGHNPSLKDIERT"
     aSModule        5939..7030
                     /domains="nrpspksdomains_pks1_PP-binding.1"
                     /domains="nrpspksdomains_pks1_Thioesterase.1"
                     /final_module
                     /incomplete
                     /locus_tags="pks1"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        6329..7030
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_Thioesterase.1"
                     /evalue="1.30E-44"
                     /label="pks1_Thioesterase.1"
                     /locus_tag="pks1"
                     /protein_end="2148"
                     /protein_start="1914"
                     /score="145.1"
                     /tool="antismash"
                     /translation="RHLFMIPDGSGSATSYTEISDLGSDVAVWGMFSPFMRTPEEYKCG
                     VYGMATKFIEEMKRRQPEGPYAVSGWSAGGVIAYEIVNQLTKAGDEVSHLLIIDAPCPI
                     TIEPLPAGLHAWFAEIGLLGEGDGEAKKIPSWLLPHFAASVTALSNYTADPIPKDKCPK
                     VTTIWCEDGVCKLPTDPRPDPYPTGHALFLLDNRTDFGPNRWDEYLDIEKMTFHHMPGN
                     HFSMMHGDLAKQ"
     CDS_motif       6509..6583
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0007"
                     /evalue="9.40E-12"
                     /label="NRPS-te1"
                     /locus_tag="pks1"
                     /protein_end="1999"
                     /protein_start="1974"
                     /score="37.3"
                     /tool="antismash"
                     /translation="QPEGPYAVSGWSAGGVIAYEIVNQL"
     polyA_site      7482
                     /gene="pks1"
ORIGIN
        1 atccacctaa gctaggtggc agccctatca cccaattacc ctctcccaaa acggaaatat
       61 aacgcttatc ttccatgaaa ccttcggtca ccgaggatcg aacgatgccg gactaattgc
      121 agaggtactg tatcgagacc agggagtggg agtgagttca gttttctttc tacacacacc
      181 cccccttgct cttagtctcg acgcctgagg tacaatgtct agatatcaca cacatgggac
      241 tatgtaggca ctgccttcgt gttatattta gttcagacta gcttcattga gtggtgttgt
      301 gttgatctga tatatataag tgaaagacac ctcgaccgcc cccttaggct tggaatacca
      361 cacaaacaac gaacaacacc agcctcaccc atcacagaca tgatggcgga ccagatggca
      421 tttctcctct tcggagacca gagtctcgac acacacggct tcttggccga cttctataga
      481 cgagggaacc cgagtgttct atcaaaagaa ttcctccggc taaccggcga tgctctgaga
      541 gacgagatag accgactacc tcgagaagaa agacgaaaga taccgatatt ccgaacacta
      601 cagcaactca acgagagata tcacgcacaa cagatcaagt tccccgggat cgacagtgcc
      661 ctactatgca tagcgcagct ggcacactac atcgagtatg agaattcacc aagctcatga
      721 cggtcatgag tcttcactaa cacgatgcta tagccggtcc gagaaggagt atgaagatgt
      781 gaccgatcac aggaacacga agctctctgg gctttgcact ggcctcttcg ccgcaacggc
      841 cattgcgtcg agctattcgc tctcgaccct tctcccgatc gccattcaag tcgtgttgat
      901 ggcatttaga acaggatctc atgtagcttc gttagcggaa aggctcagcc cctcaactga
      961 aaagtcggaa agctggacct atgttctccc tgccgctcag gaagcgcagg cgaagtccat
     1021 tctcgcagag tttcacgagt cagaggtaac gtagtttgaa cgcctgattt gccgtatcct
     1081 atctaacatt ttacaaggga atctcactgg ctgcgcaagc ttatgttagc gctgtctccg
     1141 cgaataacat tgcgatatct ggtccgccgg ccacactgca ggctctattt agcaaggatt
     1201 tgtttgaatc gagacctaca gccattccag tttatggccc atatcatgcc ccacatttac
     1261 atgggctagt agacatcagg aagatgcttc gacttgatga cgaggctgtc actatagccc
     1321 ttgcgggatc aaagccccga agtgttgtta tgtcctgcgt aagtggtgaa ccgttccgcg
     1381 agacggacac caaatccctt ctcacggccg tggttcatga gattctcaat gaatcactca
     1441 tattccataa gaccctcagc ggtaacgcca atgaggcacg gggtttcaag ggatcccgca
     1501 tcctggtgat cccatatggc ccaacccagg ccgcgagcac gttagccaac gtcttgcgct
     1561 cacagacgaa ggcagaggtg atacttagac cagcgcctca agtttctcgt gagaccacgg
     1621 catcctcaat cgggaaccat ggatcatcag gcaactgcaa actcgctatc gtcggtatgg
     1681 caggccgctt cccagattca gctagtcatg aagcactctg ggagcttctc gagaagggca
     1741 tagatgctca ccgtgtcatt ccggccgatc gattcccggt tgatactcat tacgatccga
     1801 ccggaaaggc aatcaacacc agtcacacgc cctacggcaa ttggattgag aaccctggcc
     1861 actttgaccc gcggttcttc aacatgtctc ctcgcgaggc tttccagact gatccaatgc
     1921 agcgtctagc cttgacgact gcttacgagg ctctcgagat gtccggctac gtgccgaacc
     1981 ggacaccttc tacgagacta gaccgcattg gtacattcta cggccagacg tctgacgact
     2041 ggcgtgaaat caacgccgca caggaggttg atacatacta catcactggc ggtgtccgcg
     2101 cttttggtcc aggtcggatc aattaccact tcggtttcag tggtcccagt ctaaatatcg
     2161 acactgcttg ctcgtcaagc gccgccgcac ttcaagttgc ctgcacgtcc ttgagggcaa
     2221 aggagtgcga caccgccatc gttggcggtc tgtcctgcat gacgaactca gatattttct
     2281 ctgggctgag tcgtggccag ttcctttcca aggagcacaa ctgtaacacg ttcgacaatg
     2341 atgcagatgg ttattgccga gctgatggtt gtgcttcggt gattgtcaag cgccttgatg
     2401 acgccctagc tgacaaggac aatatccttg cggtcattct cggaactcag acgaaccatt
     2461 ctgctgacgc catttctatt actcaccctc acggaccaac gcaatctatt ctgtcttcct
     2521 ccatcttgga tgaggccggt gtggatcccc ttgatgttga ctatgttgaa atgcacggaa
     2581 ctggcaccca ggccggcgat ggtaccgaga tggtgtccgt taccgacgtt ttcgccccag
     2641 ctaaccgaca taggcctgcg gacaggcctc tctaccttgg cgcggtaagt aaccatagac
     2701 tggcacccct ttcaccccgg aaatagaagt cgaaatagct aacgtttatt cccacatata
     2761 ggtgaaagca aatgtcggcc acggtgaagc tgcttctggc gtcactgccc tcatcaaggt
     2821 tttgatgatg ctcaagaaga atgcgatccc accgcatatc ggtatcaaga aggagatcaa
     2881 caagaccttc cccaaagatc tcggcgaacg tggcgttaat attgccttcc acaagactcc
     2941 ctttaccaga aaggacggaa agcctagaaa ggttttcgtc aacaacttca gtgctgctgg
     3001 tggtaacact ggtcttctac tcgaggaagg accccgttac aagacggctg ttgttgaccc
     3061 acgcagccaa catgtcatta ctatcactgc gaaatcaaag gccgccatga tccgaaacgc
     3121 tgagaacctt gtcaaatgga tggaagagaa cccctctact tctgtttctc atgtggcata
     3181 cactacgaca gctcgcaaga ttcagcacta ctggcgcatg aacgtggttg cctcaagttt
     3241 ggttgaagcc caagaagcaa tcaaggagcg tcttaagtct aacttcgtac ccgtctctcc
     3301 tgagcagcct aaggttgcct tcctgttcac cggccagggt tctcactacg ctggccttgg
     3361 cagggacctt tacgctcact actctatctt ccgccaggct atcaatgagt acaaccaact
     3421 tgctctgatt cacggattcc catcattcct ccctctcatt gacggcagcg agcccgaggt
     3481 tacaaagctc tctcctgtcg ttgtccaact tggcctcgct tcttttgaga tggctcttgc
     3541 cagactttgg atctcctggg gcattcaacc cagtgccgtc ctaggtcaca gtttgggcga
     3601 gtatgccgct cttcacgttg ctggtgttct atctgctagc gatactatct acctcgtcgg
     3661 cgctcgtgct aagttgctcg ttgaaaaatg cactgctggc actcacgcaa tgttggctgt
     3721 ccagggctct gtcgagactg tcacggaggc tcttggtgag cgtgccgcca cgaccaacat
     3781 tgcttgcatc aatggcccac gagagaccgt tctcagtggt acctccaccg aggtagccga
     3841 gatcgctgag cagcttggca atgctggctt caagtgcacc caattgaagg tgccttttgc
     3901 cttccactct gctcaggttg agcctatctt ggatgacttt gagaagctgg cccgctcggt
     3961 ccgcttctac gaacctaaga tccctgtcat ttctcctctt catggtaaac tcctcgaggg
     4021 cgagcctatt aaccctgctt accttcgtaa ccatgcccga gacgctgtca acttccttgg
     4081 cggtcttgtc tctgctcaat cctctggcgt catcgatgag aagactgcct ggctcgaggt
     4141 cggccctcat ccagtctgtg ccaatatggt caaggcagcc tttggtgcga ccacaattgc
     4201 tgtcccaacc ctgcgccgca atgaagctac ctacaagact ctcagtgcga cgctatgcac
     4261 tcttcactcc gctggtttga acatcgactg gaacgaattc caccgtgact ttgatgcatc
     4321 agttcgtctt ctagacctgc ctgcttactc gttcgacttg aagaactact ggctgcagta
     4381 cactggtgaa tggtgtctta ccaagaaccg cgctgctttg cccgctccca ccaaggcaat
     4441 tgagccagct aagccgaagt tatctactac taccgtccag aagattacca aggaggaagt
     4501 caagggtgaa atcgccatcc ttgagactga atcggactta gccgaagaga accttcgaaa
     4561 gatctgcttg ggtcaccgtt gcaatggcac tacactcacc ccatcgactc tatatgctga
     4621 catggccgag actatctgtg agtatggtta taagctcttg cgacccgaga cggaagatat
     4681 tggcatgaac gtcgcccaca tggaggtgac caagaccctc attttcgacg ccaaagctgc
     4741 aagccaaatg ttgcgcatgg aggttaaggt caacgctgct gagaattacg ccgacgtcac
     4801 ctggacaagt ggtgaaggcg ccagaaagac cgaacacgct gtctgcaagg tcctattcgg
     4861 tgacaagcag caatggctag acgaatttga gcgcgtcaac tacctgatta agggacgcat
     4921 cgatgccctc aaggctgccg aggagcgtgg tgaagcctca aagatcggtc gtggccttgc
     4981 atacaagctg tttaccgcac tcgttgacta ctcccatcag tacaagggca tggagtctgt
     5041 catcctaagc agcgagactt gcgaagcaac tgccaaagtt gtcttccaaa cttcgcccga
     5101 ggatggaact ttccgcactc ctccctactg gattgactct gtctgccaca tctctggttt
     5161 catcgtgaac ggcaccgatg ctgttgactc ccgtgagcaa gtctacatat cccatggctg
     5221 gggcagcatg agatttgccg agcgcctcag caggacgaag acttaccaga cctacatccg
     5281 catgcagccc gttaagggca ccaagatgat ggctggtgac gcctatgttt tcgatggtga
     5341 taaactcatt ggtatcggtg gcgaaatccg cttccaggcc atccctcgca aggttctcaa
     5401 cgtcgtccta cctcctcccg gcggtgctgc tgctgccgcc cgctccgccc cggctgctaa
     5461 acctgctgct gctaccaagg cacctgtaaa ggagaagaag caggttacca gctccaacct
     5521 ccccgccgtc aacaagaaac tctctcaaac ctcagttgtc tcccaagtca tggacatcat
     5581 ttcgaaagag actggcgtta gccatgatga gttggcagat aacattgcct tcgctgatct
     5641 cggtgtcgac tcgttgatgg gacttacaat cagtggtcgt cttcgtgagg acctcgaact
     5701 caatgtcgac tctcacgcgt tcaatgacca tgctacaatt ggtgccttca agaagttcct
     5761 cgcccaattt gagactgcac cgacaaccgt cgttgagtcc agcggctcaa gtggcagctc
     5821 cgatagcgat gatgacattg actccgactc tgaagttacc accccgggcg agagtgataa
     5881 gggctcggta aagggcgatg cacccgccga ggccggaagc aacagcgaac tccaacagat
     5941 tgtccgcgag acgatctgtg ctgagatggg tgtagaggtt gaggagatca tcgccgctcc
     6001 cgacttagct gcgcttggca tggactctct tatgagcttg tccatcctgg gtatccttcg
     6061 tgaaaagacc ggcctcaaca tcccatccga cctcttaggc cacaaccctt ctctgaagga
     6121 tatcgagcga acccttggta ttgaagacaa gcccaagcgt gcagcaccag ccccgaagcc
     6181 tgctgccccc aaggtatcta agaagaccga atcaaaggct gcaactaaga ccgagactac
     6241 tgtaacaaag atctcgtcgg ccgttgatgc ttaccctcac aggaaggcaa catcggtcct
     6301 tcttcagggc aaccaccgta ccgctactag acacctcttc atgatccctg acggaagtgg
     6361 tagtgctacc tcctacaccg agatctcgga tctcggctcc gatgttgccg tctggggtat
     6421 gttctcgcct ttcatgagga cccccgagga gtacaagtgt ggtgtatacg ggatggccac
     6481 gaagttcatc gaggagatga agcgtcgcca gccggagggt ccctacgctg tttcgggttg
     6541 gtctgcgggc ggcgtgatcg cctatgaaat cgtcaaccag ctcaccaagg ccggcgatga
     6601 agtctctcac cttcttatca tcgatgcgcc ttgccccatc accattgagc ccttgcctgc
     6661 tggtctccac gcctggttcg cggagatcgg tctccttgga gaaggtgatg gtgaagctaa
     6721 gaagattcct tcttggctcc tccctcactt tgctgcatcg gtcaccgcct tatccaacta
     6781 taccgccgac ccgatcccca aggataagtg ccccaaggtc actaccatct ggtgtgaaga
     6841 tggtgtctgc aagttgccca ccgacccacg gcctgacccc taccctaccg gccacgccct
     6901 cttcctcctt gataaccgaa ccgacttcgg gcctaaccgc tgggatgagt accttgacat
     6961 cgagaagatg accttccacc acatgcctgg caaccacttc tcaatgatgc acggcgacct
     7021 tgccaagcaa cttggtggtt tcttgaagga aggcattcac gcctaatgaa ggaaggcatt
     7081 cacgcctaag ctggttgtcg gccccaagga tcccattcac tcttgtcttc tattactttt
     7141 gtaagaccac acatacctca tcctgtacat aataactatt cttcatgttc acgctccttt
     7201 ggttctctct tgtttgggca tggctgatga cgatccgggc ttataataat gtcggactct
     7261 cattcagagg agtcggcgtt atatgtggaa tatgaaagat cgggagaact tatccataga
     7321 catgttgtgt gtttcgtagc tgcggatatc gttgatacat acacactatc gggctaccat
     7381 gatgtattca atcgcagcct tctcttagat gagaagttca acgcacactc aaagccgagt
     7441 attaaagaag cttatatata gaattccttc acgttctcta gtactcatcc tctagtatcg
     7501 actaaacttt ctgattctga cagtgatgag tcctcttggt ggtaacagt
//
