LOCUS       BGC0001258              7560 bp    DNA     linear   PLN 14-APR-2003
DEFINITION  Glarea lozoyensis polyketide synthase 1 (pks1) and gsp genes,
            complete cds.
ACCESSION   BGC0001258
VERSION     BGC0001258
KEYWORDS    .
SOURCE      Glarea lozoyensis
  ORGANISM  Glarea lozoyensis
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Leotiomycetes; Helotiales; Helotiaceae; Glarea.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:13:04
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7560
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     5'UTR           1..43
                     /gene="pks1"
     gene            1..7560
                     /gene="pks1"
     mRNA            join(1..345,402..690,1091..2813,2864..2959,3301..7560)
                     /gene="pks1"
                     /product="polyketide synthase 1"
     protocluster    1..7560
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[43:7265]"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      44..7265
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..7560
                     /SMILES="CC(=O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7560
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001258"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7560
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             join(44..345,402..690,1091..2813,2864..2959,3301..7265)
                     /NRPS_PKS="Domain: SAT (10-246). E-value: 5.7e-76. Score:
                     247.1. Matches aSDomain: nrpspksdomains_pks1_SAT.1"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (380-809). E-value:
                     1.1e-139. Score: 457.9. Matches aSDomain:
                     nrpspksdomains_pks1_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (908-1208). E-value: 1.4e-87.
                     Score: 285.6. Matches aSDomain:
                     nrpspksdomains_pks1_PKS_AT.1"
                     /NRPS_PKS="Domain: PT (1288-1604). E-value: 2.5e-127.
                     Score: 416.5. Matches aSDomain: nrpspksdomains_pks1_PT.1"
                     /NRPS_PKS="Domain: ACP (1650-1718). E-value: 2.2e-11.
                     Score: 35.8. Matches aSDomain: nrpspksdomains_pks1_ACP.1"
                     /NRPS_PKS="Domain: PP-binding (1773-1835). E-value:
                     2.4e-12. Score: 39.1. Matches aSDomain:
                     nrpspksdomains_pks1_PP-binding.1"
                     /NRPS_PKS="Domain: Thioesterase (1885-2110). E-value:
                     6.7e-37. Score: 119.9. Matches aSDomain:
                     nrpspksdomains_pks1_Thioesterase.1"
                     /codon_start=1
                     /gene="pks1"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     itr_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 187.4; E-value:
                     7.5e-57)"
                     /gene_kind="biosynthetic"
                     /product="polyketide synthase 1"
                     /protein_id="AAN59953.1"
                     /sec_met_domain="itr_KS (E-value: 3.5e-159, bitscore:
                     516.1, seeds: 96, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.6e-89, bitscore: 285.6,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.2e-14, bitscore:
                     39.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGTSNQKVFFLFGDQSLDTYGFLANFLHRSSHSLLSKTFIEQAS
                     DALCREVNALSNLERNKIPRFSSLEDLNERYHISGIKHSGIESALLCATQIAHYLDRAE
                     KVPEDSASPNNTTLVGLSTGLFAAAAISCSASLSALIQVGVQATVLAFRTGSYVAALAD
                     QLGGANDNANPWAYSISADEAQAASKLDEFHAEKAISHASRAYISATTPDSVAISGPPS
                     TLEALWASDAFSVTPTPLPLYGPYQASHLHPELQIEKVLGLKNSRISDVLESSRPNSRI
                     LSSATGQSFEEQSALQLFSAVAENVLTKSSHLQNVLDGCKSESTGRTLVIPVGPTSFAT
                     TVVDSLRSASGSDVVLRRAPFGNNEPAPFAGSGSSSTGKLAIVGMAGRFPDAASHELLW
                     ELLEKGLAVHREVPPDRFPVKTHVDPSGKTVNTSHTPFGCWIENPGLFDPRFFNMSPRE
                     AFQTDPMQRMALSTAFEALEMSGYTPNRTPSTRLDRVGTFYGQTSDDWREINAAQEVDT
                     YFITGGVRAFGPGRINYHFGFSGPSFNVDTACSSSAAALQLAYTSLCAKDCDTAIVGGL
                     SCMTNSDIFAGLSRGQFLSKTGPCATFDNDADGYCRADGVGTIIVKRLEDAISDKDNVL
                     AVILGSATNHSADAVSITHPHGGTQEILYRSILRKAGVDPLDIDYVEMHGTGTQAGDGT
                     EMKSVTNVFAPADRKRTAEQPLYLGAVKANVGHGEAASGITALIKVLMMLQKNAIPPHV
                     GIKNTINVGFPKDLAERNVNIAFHKTPLRARAGMPRRVFLNNFSAAGGNTGILLEDGPT
                     YKNATVDPRSMHVVSVTAKSKSAMFRNASRLVDYLEANPKTTISDLAYTTTARRIQHNW
                     RMTFSASDIQQAKSIIKSKMNDTFVPVHSVPPKVAYTFTGQGSHYAGLGKDLYNNSSVF
                     RENILEFDRIARIQGFPSFLPLIDGSVTDVSTLSPVVVQIGMVCFEVAMARLWASWGVN
                     PSVLVGHSLGEYSALCVSGVLSASDTIYLVGARAQLLVEKCTANTHAMLAVQGSVDALN
                     EALPEMAGSLSVACINGPRETVLGGEAGAMADLAEKLTQSGFKCTKLQVPFAFHTAQVD
                     AILDDFEKLAASVRFGSANIPLISPLLGRPLSEGESIDPTYLRNHAREAVNFLAGLTSS
                     QELGIIDEKTVWIEVGPHSVCSGMVKSTFGATTITAPSLRRNEEAYKTISASLCALQNA
                     GMNIDWNEYHRDFSDSVRLLSLPTYSYDDKNYWIQYEGDWSLNKGRKAAGALPAPEIVK
                     SSLSTTSVHEVVKETISDHVATVVTETDIARPDLRPLVTGHVVNGSYLCPSSLYGDMAM
                     TVCEYGYKLIDPDVKDLVMDITHMEVPKSLIADPAGKSQILTLTATIDINRRSASLVFS
                     SGSDKSKVEHAHCRVLFGTREAYLSEFQRQSYLIQSRVDWLKKAESEGEAHKIGRGLAY
                     KLFAALVDYDKRYRGMEEVILHSENMEATSRVVFQTTPEDGTFNCSPYWIDSVAHISGF
                     IVNGSDAIDSKENVYISHGWESFRIAEPLSATKTYRSYVRMQPVEKKVLQGDVYIFDGD
                     RIIGMVGGLKFQCIQRRVLNMLMPPVGAAAASKPAARAAPPQVTTKKPVKTAQVTKDSI
                     SKVNQRLTSVTSAVMNILATEIGVGLDELVDNLAWNDLGCDSLMSLTVSGKLREDLEID
                     VPSSAFTDNATIGEFKAFLSKYERKESVVTTTSDSSSSHDTATPELEDSEDTEVTTPSD
                     TSEEEKEPSNDSELGNIIRTTIAGEMGVDIDEIIDLSDLTEIGMDSLMSLSILGILRER
                     TGMNLPADLLANNPSIKAIEKSLNIGGTKAAKPAKAPAKKPSAPAKSISATTERLATSV
                     LLQGNSKKATKHLWMVPDGSGSATSYTEISEIGPNVAVWGLNSPYMKVPEEYKCGVIGM
                     ASSFISEIKRRQPVGPYLLAGWSAGGVIAFEAVNQLIKNGDEVEQLIIIDAPCPDIIEP
                     LPASLHRWFGEIGLLGDGDLSKLPKWLLPHFAASVTALSNYSAELIDPKKSPFVTAIWC
                     EDGVCHLPTDPRPDPFPYGHAQFLLDNRTDFGPNLWDKYLNGERFVTKHMPGNHFSMMK
                     GDLVSSYPSHNSLQ"
     aSDomain        join(74..345,402..690,1091..1237)
                     /aSDomain="SAT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_SAT.1"
                     /evalue="5.70E-76"
                     /label="pks1_SAT.1"
                     /locus_tag="pks1"
                     /protein_end="246"
                     /protein_start="10"
                     /score="247.1"
                     /tool="antismash"
                     /translation="FLFGDQSLDTYGFLANFLHRSSHSLLSKTFIEQASDALCREVNAL
                     SNLERNKIPRFSSLEDLNERYHISGIKHSGIESALLCATQIAHYLDRAEKVPEDSASPN
                     NTTLVGLSTGLFAAAAISCSASLSALIQVGVQATVLAFRTGSYVAALADQLGGANDNAN
                     PWAYSISADEAQAASKLDEFHAEKAISHASRAYISATTPDSVAISGPPSTLEALWASDA
                     FSVTPTPLPLYGPY"
     CDS_motif       448..513
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0001"
                     /evalue="3.40E+00"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="pks1"
                     /protein_end="138"
                     /protein_start="116"
                     /score="0.9"
                     /tool="antismash"
                     /translation="TLVGLSTGLFAAAAISCSASLS"
     aSDomain        join(1640..2813,2864..2959,3301..3317)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="1.10E-139"
                     /label="pks1_PKS_KS.1"
                     /locus_tag="pks1"
                     /protein_end="809"
                     /protein_start="380"
                     /score="457.9"
                     /tool="antismash"
                     /translation="AIVGMAGRFPDAASHELLWELLEKGLAVHREVPPDRFPVKTHVDP
                     SGKTVNTSHTPFGCWIENPGLFDPRFFNMSPREAFQTDPMQRMALSTAFEALEMSGYTP
                     NRTPSTRLDRVGTFYGQTSDDWREINAAQEVDTYFITGGVRAFGPGRINYHFGFSGPSF
                     NVDTACSSSAAALQLAYTSLCAKDCDTAIVGGLSCMTNSDIFAGLSRGQFLSKTGPCAT
                     FDNDADGYCRADGVGTIIVKRLEDAISDKDNVLAVILGSATNHSADAVSITHPHGGTQE
                     ILYRSILRKAGVDPLDIDYVEMHGTGTQAGDGTEMKSVTNVFAPADRKRTAEQPLYLGA
                     VKANVGHGEAASGITALIKVLMMLQKNAIPPHVGIKNTINVGFPKDLAERNVNIAFHKT
                     PLRARAGMPRRVFLNNFSAAGGNTGILLED"
     aSModule        1640..6044
                     /complete
                     /domains="nrpspksdomains_pks1_PKS_KS.1"
                     /domains="nrpspksdomains_pks1_PKS_AT.1"
                     /domains="nrpspksdomains_pks1_PT.1"
                     /domains="nrpspksdomains_pks1_ACP.1"
                     /iterative
                     /locus_tags="pks1"
                     /monomer_pairings="mal -> mal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       2117..2164
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0002"
                     /evalue="1.10E-05"
                     /label="PKSI-KS_m3"
                     /locus_tag="pks1"
                     /protein_end="555"
                     /protein_start="539"
                     /score="17.5"
                     /tool="antismash"
                     /translation="GPSFNVDTACSSSAAA"
     CDS_motif       2306..2344
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0003"
                     /evalue="6.00E-04"
                     /label="PKSI-KS_m4"
                     /locus_tag="pks1"
                     /protein_end="615"
                     /protein_start="602"
                     /score="12.9"
                     /tool="antismash"
                     /translation="FDNDADGYCRADG"
     CDS_motif       2537..2584
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0004"
                     /evalue="2.10E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="pks1"
                     /protein_end="695"
                     /protein_start="679"
                     /score="10.7"
                     /tool="antismash"
                     /translation="YVEMHGTGTQAGDGTE"
     CDS_motif       2654..2698
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0005"
                     /evalue="1.10E-02"
                     /label="PKSI-KS_m6"
                     /locus_tag="pks1"
                     /protein_end="733"
                     /protein_start="718"
                     /score="8.8"
                     /tool="antismash"
                     /translation="GAVKANVGHGEAASG"
     CDS_motif       3615..3662
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0006"
                     /evalue="2.10E-04"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="pks1"
                     /protein_end="924"
                     /protein_start="908"
                     /score="14.1"
                     /tool="antismash"
                     /translation="FTGQGSHYAGLGKDLY"
     aSDomain        3615..4514
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_PKS_AT.1"
                     /evalue="1.40E-87"
                     /label="pks1_PKS_AT.1"
                     /locus_tag="pks1"
                     /protein_end="1208"
                     /protein_start="908"
                     /score="285.6"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="FTGQGSHYAGLGKDLYNNSSVFRENILEFDRIARIQGFPSFLPLI
                     DGSVTDVSTLSPVVVQIGMVCFEVAMARLWASWGVNPSVLVGHSLGEYSALCVSGVLSA
                     SDTIYLVGARAQLLVEKCTANTHAMLAVQGSVDALNEALPEMAGSLSVACINGPRETVL
                     GGEAGAMADLAEKLTQSGFKCTKLQVPFAFHTAQVDAILDDFEKLAASVRFGSANIPLI
                     SPLLGRPLSEGESIDPTYLRNHAREAVNFLAGLTSSQELGIIDEKTVWIEVGPHSVCSG
                     MVKSTFGATTITAPSLRRN"
     CDS_motif       3849..3965
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0007"
                     /evalue="3.30E-15"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="pks1"
                     /protein_end="1025"
                     /protein_start="986"
                     /score="48.1"
                     /tool="antismash"
                     /translation="GVNPSVLVGHSLGEYSALCVSGVLSASDTIYLVGARAQL"
     aSDomain        4755..5702
                     /aSDomain="PT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_PT.1"
                     /evalue="2.50E-127"
                     /label="pks1_PT.1"
                     /locus_tag="pks1"
                     /protein_end="1604"
                     /protein_start="1288"
                     /score="416.5"
                     /tool="antismash"
                     /translation="TTSVHEVVKETISDHVATVVTETDIARPDLRPLVTGHVVNGSYLC
                     PSSLYGDMAMTVCEYGYKLIDPDVKDLVMDITHMEVPKSLIADPAGKSQILTLTATIDI
                     NRRSASLVFSSGSDKSKVEHAHCRVLFGTREAYLSEFQRQSYLIQSRVDWLKKAESEGE
                     AHKIGRGLAYKLFAALVDYDKRYRGMEEVILHSENMEATSRVVFQTTPEDGTFNCSPYW
                     IDSVAHISGFIVNGSDAIDSKENVYISHGWESFRIAEPLSATKTYRSYVRMQPVEKKVL
                     QGDVYIFDGDRIIGMVGGLKFQCIQRRVLNMLMPP"
     aSDomain        5841..6044
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_ACP.1"
                     /evalue="2.20E-11"
                     /label="pks1_ACP.1"
                     /locus_tag="pks1"
                     /protein_end="1718"
                     /protein_start="1650"
                     /score="35.8"
                     /tool="antismash"
                     /translation="AVMNILATEIGVGLDELVDNLAWNDLGCDSLMSLTVSGKLREDLE
                     IDVPSSAFTDNATIGEFKAFLSK"
     aSDomain        6210..6395
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_PP-binding.1"
                     /evalue="2.40E-12"
                     /label="pks1_PP-binding.1"
                     /locus_tag="pks1"
                     /protein_end="1835"
                     /protein_start="1773"
                     /score="39.1"
                     /tool="antismash"
                     /translation="IRTTIAGEMGVDIDEIIDLSDLTEIGMDSLMSLSILGILRERTGM
                     NLPADLLANNPSIKAIE"
     aSModule        6210..7220
                     /domains="nrpspksdomains_pks1_PP-binding.1"
                     /domains="nrpspksdomains_pks1_Thioesterase.1"
                     /final_module
                     /incomplete
                     /locus_tags="pks1"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        6546..7220
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks1_Thioesterase.1"
                     /evalue="6.70E-37"
                     /label="pks1_Thioesterase.1"
                     /locus_tag="pks1"
                     /protein_end="2110"
                     /protein_start="1885"
                     /score="119.9"
                     /tool="antismash"
                     /translation="HLWMVPDGSGSATSYTEISEIGPNVAVWGLNSPYMKVPEEYKCGV
                     IGMASSFISEIKRRQPVGPYLLAGWSAGGVIAFEAVNQLIKNGDEVEQLIIIDAPCPDI
                     IEPLPASLHRWFGEIGLLGDGDLSKLPKWLLPHFAASVTALSNYSAELIDPKKSPFVTA
                     IWCEDGVCHLPTDPRPDPFPYGHAQFLLDNRTDFGPNLWDKYLNGERFVTKHMPGNHFS
                     MMK"
     CDS_motif       6723..6797
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks1_0008"
                     /evalue="6.50E-12"
                     /label="NRPS-te1"
                     /locus_tag="pks1"
                     /protein_end="1969"
                     /protein_start="1944"
                     /score="37.8"
                     /tool="antismash"
                     /translation="QPVGPYLLAGWSAGGVIAFEAVNQL"
     3'UTR           7266..7560
                     /gene="pks1"
ORIGIN
        1 atcaaccttt ccaattcaca actggaatac acagtccttg aagatgtcgg gaacctctaa
       61 ccagaaggtt ttttttctct tcggggacca atcacttgac acatatggct tccttgcgaa
      121 tttccttcat cgcagctcgc atagtctact gtcgaagacc ttcattgagc aggcctccga
      181 tgccctttgc cgcgaagtaa atgctttatc gaacttggaa cgaaataaga tacccagatt
      241 ttccagcctt gaggatctca acgaacgata tcatattagt gggatcaagc actccggtat
      301 cgagagtgcg ttattatgtg ccactcaaat tgcacactac cttgagtaag tctcccaacc
      361 ccatgcaatg gcaagtgtaa ttgctaacat ggcgcctata gccgtgcaga aaaggtgcca
      421 gaggactctg cttctcccaa caacacaacc ctggtaggct taagtacagg actatttgcc
      481 gcggccgcca tatcttgctc ggcttcattg tctgcactta ttcaggttgg agttcaagcg
      541 acagtcctcg cattcagaac tggctcctat gttgctgcgt tggctgatca acttggtgga
      601 gccaacgaca atgccaatcc gtgggcatac tccatatcag cagatgaagc gcaggccgca
      661 tcgaagcttg atgagtttca tgctgaaaag gtaagcttaa ctctctccta aatctaatcc
      721 taataaattt ccttcaagca attgacgaaa tatcaatatt tcatcaatcg ttttggagaa
      781 acctcttacg gacctgtgac ttgcatcggc cactttgagt gtgctctata ttggcatatt
      841 cctctggatc aatcgatgct caattcttca aagtgattga cgaaatatcg atattttgtc
      901 aatcgttttg gagaagccta atgctttgac ctatcacttg tgtcggccac tatgagcgtg
      961 ctctaaatgg gcatattccg gtgagcctat tcaaagcagt ggacgaaata tcgatatttc
     1021 gtcaattgct ctgagaaatc atcatcgtca atcacttatc tatcacgaga gctatactaa
     1081 cattgctcag gcgatctccc atgctagtag ggcttacatt agcgctacca caccagactc
     1141 tgtagccatt agcggaccac cctccaccct tgaggctcta tgggctagtg atgcattctc
     1201 ggtaacacca acgccattgc cgctatatgg cccatatcag gcgtcacatc ttcacccaga
     1261 gctccaaatc gaaaaggtgc taggtcttaa gaactctcgt atttccgatg tattggagtc
     1321 ctccagacct aattctcgaa tcttgtcatc agcaacagga cagtcatttg aggagcagtc
     1381 cgctctacag ctattctcgg cggttgctga gaatgttctc accaagtcgt ctcacctaca
     1441 gaatgttctt gatggatgca aatctgagag cactggccga actttggtca tacctgtagg
     1501 cccgacatct ttcgcgacta cagtcgtgga ttcgttgagg tctgcaagcg ggtcggatgt
     1561 ggttctccga agagcgccat tcggcaacaa cgaaccagcc ccttttgctg gcagtggatc
     1621 atctagcacc ggaaagcttg ccattgttgg catggctgga aggtttccag atgccgcaag
     1681 ccatgagttg ctatgggagc tcctagagaa gggactcgct gtacacagag aagttccccc
     1741 agacagattc ccagtcaaga cgcacgtcga cccttctgga aagactgtga acacaagtca
     1801 cactcctttt ggttgctgga ttgagaaccc gggccttttc gaccctcgat tcttcaacat
     1861 gtctcctaga gaggccttcc agacagaccc catgcagagg atggctcttt ccacggcttt
     1921 cgaggctttg gagatgagcg gatatacacc aaaccgtaca ccttccacca gacttgatcg
     1981 agttggtacc ttttacgggc aaacctcgga cgactggcgt gagattaatg ccgctcagga
     2041 ggtcgatacc tactttatta ccggtggagt aagagcgttc gggccaggta gaatcaatta
     2101 ccactttggg tttagtgggc ctagtttcaa tgtcgatact gcctgttcat ctagcgcggc
     2161 agcacttcag cttgcctata cctccctctg cgctaaggac tgcgacactg ctatagttgg
     2221 cggtctttcc tgtatgacga acagcgatat ttttgctggt ctgagtcgtg ggcaattcct
     2281 ttcaaaaaca ggaccttgcg ctacatttga caacgacgca gacggatatt gccgagctga
     2341 tggcgtaggg acgattatcg tcaaacgtct tgaagacgcc atttccgata aagacaacgt
     2401 tctagctgtc atcttaggtt cggcaacgaa tcattcagct gatgcagtct caattactca
     2461 cccacacgga ggaactcaag aaatactcta tcgctctatc ctccgcaaag ccggtgttga
     2521 tcctcttgac atcgactacg ttgaaatgca cggcaccggc acacaggcgg gcgacggcac
     2581 agaaatgaag tctgtcacga acgtctttgc accagcagac cgtaagagga cggcagagca
     2641 acctctgtac cttggtgctg tcaaagcaaa tgtcggccat ggtgaagcag cttctggtat
     2701 cactgcactg atcaaagtcc tcatgatgct gcagaagaac gccatcccgc ctcatgtagg
     2761 tattaaaaac actataaacg tcggtttccc caaggatttg gccgagcgca atggtgagtt
     2821 tctcaacttt gaggggatta aagtcataac taacattcga cagttaacat cgcattccat
     2881 aaaactcctc ttcgggctcg agctggcatg cccagacgtg tgtttttgaa taatttcagc
     2941 gctgctggag gcaatactgg tgggcatttt cttttatctt atgtggttct tttaaaatca
     3001 tttttttggt gttttgggag ttgaagagaa gggaggttag gggacccaca ccgtggtggc
     3061 agaatcactc gccacccctg agcggccccc accgcctttt cgcgatggaa cagaatctga
     3121 ctgccctggc aatcgaaaca cgataatccc caaaacaatt gacgaaatct cacatgtttc
     3181 gtgaattggt tacaggacat atgcgagatt tggtcaattg ttttgggcaa atataacacc
     3241 ctaagagcca aattcgtagg gtactatcta acgagaggac gaagtactga cctgttacag
     3301 gaattctact agaggatggc ccaacataca agaatgccac agttgatcct cgatcaatgc
     3361 atgttgtatc ggttacagca aagtccaagt ctgcgatgtt tcgaaacgct tcccgcttgg
     3421 tcgactacct cgaagccaac ccaaaaacta ccatttccga cctcgcatac accactaccg
     3481 ctcgaaggat ccagcacaac tggcgaatga ccttttcagc atctgatatt cagcaggcga
     3541 agagtatcat caagagcaag atgaatgaca ctttcgtccc cgttcactcc gtgcctccta
     3601 aggttgccta caccttcact ggccaaggct ctcattacgc tggtctcggc aaagatttgt
     3661 acaacaactc atctgtcttc cgagaaaaca ttctagagtt cgacagaatc gccagaattc
     3721 agggcttccc atcattcctt cccctcatcg acggcagcgt tactgatgtt tctacgctgt
     3781 caccagtcgt tgtccagatt ggtatggtat gcttcgaagt cgccatggct cgtttgtggg
     3841 catcctgggg agtcaaccct agcgttcttg tcggccacag cttgggtgaa tattcagcct
     3901 tgtgcgtgtc tggcgtgctc tctgccagtg acactatcta tttggtcggt gctcgggctc
     3961 agctacttgt cgagaaatgc acagccaaca ctcacgccat gttggccgtt cagggctccg
     4021 tggatgcttt gaacgaagct ctccctgaga tggcaggcag cttgagcgtt gcctgtatca
     4081 acggcccacg cgagacggtg cttggtggtg aagcaggtgc tatggcagat cttgctgaaa
     4141 agctcacgca atcgggcttt aagtgcacaa agcttcaggt accattcgct ttccacaccg
     4201 cacaagtgga tgctattctt gatgatttcg aaaaacttgc tgcctctgtt cgctttggct
     4261 ctgcaaacat tcctctgatc tctccgctcc tgggtcgtcc actttccgaa ggagaatcga
     4321 tcgaccccac ctacctccga aaccacgctc gtgaagctgt caacttcctt gctggcttga
     4381 cttcttctca agagctaggt atcatcgacg aaaagacggt ttggatcgaa gttggtcctc
     4441 attccgtctg ctccggcatg gtgaaatcca cctttggcgc aaccactatt actgcacctt
     4501 ctcttcgacg caacgaggaa gcctacaaga caatctccgc tagcttgtgc gctcttcaaa
     4561 atgccggtat gaacatcgat tggaacgaat accatcgcga cttttccgac tccgtcagac
     4621 tcctcagtct tcccacatac tcgtatgatg ataagaatta ttggattcag tacgagggag
     4681 actggagctt gaacaaagga cgcaaagctg ccggtgctct tcctgcacca gagattgtca
     4741 aatcttcatt gagcacgacc agcgttcacg aggtcgttaa agaaacgatc agcgaccatg
     4801 ttgctacagt cgtcaccgaa actgatattg cgagaccaga tctacgtccg ttggtgactg
     4861 gccacgtggt caatggctct tacctctgcc catcatctct ctatggtgat atggcaatga
     4921 cggtctgcga atatggctat aaattgattg atcctgatgt caaggatctg gtcatggata
     4981 tcacccatat ggaagttcca aaatctctga ttgcggatcc tgctggaaag tcgcagatat
     5041 tgaccctcac tgcgacaatc gatatcaatc gtcgatcagc aagtcttgtc ttttctagtg
     5101 gatcagacaa atccaaggtc gagcatgcac actgtcgtgt gctattcggt actcgcgaag
     5161 cctatctttc cgagtttcaa cgacaatctt acctcatcca gtcccgtgtg gactggctca
     5221 agaaagctga gagtgaagga gaagctcaca agattgggcg tggtctggca tacaagctct
     5281 tcgctgctct ggtcgattac gacaagcggt acagaggtat ggaggaagtt atccttcaca
     5341 gtgagaatat ggaagcgaca tcacgcgttg tcttccagac aacgccggaa gatggcacat
     5401 tcaattgcag cccatactgg atcgacagcg ttgctcacat ctcgggcttc attgtcaacg
     5461 gctctgatgc cattgactcc aaagagaatg tctacatatc tcacgggtgg gaatccttta
     5521 gaatcgccga accactctcg gctacaaaga cgtaccgctc ctacgtaaga atgcaaccag
     5581 tagagaagaa ggttcttcaa ggtgatgtct acatcttcga tggtgacagg atcattggta
     5641 tggtcggtgg tctcaagttc cagtgcattc aacgaagagt tctcaacatg cttatgccac
     5701 ctgttggagc cgctgctgct tcgaagccag ctgcccgtgc tgcaccacct caagttacca
     5761 caaagaagcc agtgaagact gctcaggtga ccaaggatag catttccaaa gtcaaccaga
     5821 ggcttacatc tgtcacctct gccgtcatga acatccttgc cacggaaatt ggagttggcc
     5881 ttgacgagct tgtcgacaac ctggcttgga acgatttggg ctgcgacagt ttaatgtccc
     5941 ttaccgttag cggaaaattg cgagaagacc ttgagattga tgttccttcc agcgccttta
     6001 cagataatgc gacaatcgga gagttcaagg ccttcctctc aaagtacgaa cgcaaggaat
     6061 ctgtagtaac gaccacttca gactcttcct ctagccatga cactgccaca ccggaattgg
     6121 aagacagcga ggacactgag gtcaccactc caagtgacac atctgaggag gagaaggaac
     6181 caagcaatga ttctgaactt ggaaacatta ttcggactac cattgcaggt gaaatgggtg
     6241 tcgacatcga tgaaataatt gatctctcgg acctcacaga aatcggcatg gatagtttaa
     6301 tgtcattgag cattctaggc atacttcgtg agcggactgg catgaatctt cctgcggacc
     6361 ttttggcgaa caatccttcg atcaaggcca tcgagaagtc gctcaatatc ggaggcacca
     6421 aagcagcaaa acccgctaag gcacctgcca agaaaccttc tgcacctgca aagtcaataa
     6481 gcgcaaccac tgagcgtcta gctacatctg tgctgctcca gggcaactcg aagaaggcaa
     6541 caaaacacct ctggatggta cctgatggaa gtggctccgc tacatcatac accgaaattt
     6601 cagagattgg acccaatgtt gccgtgtggg gcctcaattc accatacatg aaagtgccag
     6661 aagaatacaa gtgtggtgtt atcggcatgg cgtcgtcttt catctcggag atcaagcgcc
     6721 gtcagcctgt gggaccatac ttgcttgcag gatggagtgc aggtggtgtc attgctttcg
     6781 aggctgttaa tcagttgatc aagaacggtg acgaagttga acagctcatt attatcgatg
     6841 cgccatgtcc cgatatcatt gagcctctac cagcttcact tcacagatgg ttcggagaga
     6901 ttggactcct cggcgacggc gacctctcca agctaccaaa atggctctta cctcatttcg
     6961 ccgcgtctgt cacagctctg tcaaactatt ctgctgagct cattgatcca aagaagtcgc
     7021 cattcgtcac cgcaatctgg tgtgaagacg gtgtttgcca tttaccaaca gatccacgac
     7081 cagatccatt cccatacggt catgcccagt tccttcttga taatcgaact gactttggcc
     7141 cgaatctttg ggacaaatat ttgaatgggg agagatttgt taccaagcac atgccaggaa
     7201 accacttcag tatgatgaag ggagacttgg taagttccta tccttcgcac aactccttac
     7261 aatgactaac atttcttagg caaaacagct tggtaacatt atccgaggag cagtagctca
     7321 gacggcttga tattatcacc acaaatatgt aattgagctt ttttggggtg ttcaagattt
     7381 gcagcataag atggatggcg ttccggaaac tgtgttcttt gcaggcattt tgctgtcttg
     7441 ataatgttat acgagacggt atggatgctt ttggcttgct agatttattt ctgtactaat
     7501 tgcgtgttga catttcctgt atcttctaaa ttgcaatata atgtacaaat ttccgtttag
//
