LOCUS       BGC0001275              9951 bp    DNA     linear   PLN 27-APR-2005
DEFINITION  Glarea lozoyensis polyketide syntase 2 (pks2) gene, complete cds.
ACCESSION   BGC0001275
VERSION     BGC0001275
KEYWORDS    .
SOURCE      Glarea lozoyensis
  ORGANISM  Glarea lozoyensis
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Leotiomycetes; Helotiales; Helotiaceae; Glarea.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:14:00
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9951
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /db_xref="ATCC:20868"
                     /db_xref="taxon:101852"
                     /mol_type="genomic DNA"
                     /organism="Glarea lozoyensis"
                     /strain="ATCC 20868"
                     /type_material="culture from holotype of Glarea lozoyensis"
     protocluster    1..9951
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[1063:6614]"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1064..6614
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..9951
                     /SMILES="C([*])C(-O)"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..9951
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001275"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9951
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /subregion_numbers="1"
                     /tool="antismash"
     5'UTR           689..1063
                     /gene="pks2"
     gene            689..6858
                     /gene="pks2"
     mRNA            join(689..1082,1134..1216,1276..1443,1509..6858)
                     /gene="pks2"
                     /product="polyketide syntase 2"
     CDS             join(1064..1082,1134..1216,1276..1443,1509..6614)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (39-461). E-value:
                     1.9e-158. Score: 519.7. Matches aSDomain:
                     nrpspksdomains_pks2_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (572-865). E-value: 7.5e-72.
                     Score: 234.0. Matches aSDomain:
                     nrpspksdomains_pks2_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (932-1058). E-value: 1.8e-12.
                     Score: 39.8. Matches aSDomain:
                     nrpspksdomains_pks2_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (1425-1617). E-value: 5.8e-53.
                     Score: 171.4. Matches aSDomain:
                     nrpspksdomains_pks2_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (1708-1789). E-value: 3.4e-20.
                     Score: 64.1. Matches aSDomain:
                     nrpspksdomains_pks2_PKS_PP.1"
                     /codon_start=1
                     /gene="pks2"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 223.4; E-value:
                     8.5e-68)"
                     /gene_kind="biosynthetic"
                     /note="PKS2"
                     /product="polyketide syntase 2"
                     /protein_id="AAX35547.1"
                     /sec_met_domain="PKS_KS (E-value: 3.4e-160, bitscore:
                     519.7, seeds: 2284, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.3e-73, bitscore: 234.0,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 2.2e-50, bitscore:
                     157.2, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-15, bitscore:
                     44.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPFLEAEPTEHESSDMSVLSKWTASSSPSVSGKSEIDEIVVVGM
                     ACRVAGGNDTPEKFWESIMNKTVSSGEIPAMRWEPYYRRDSRNAKILDDTTSKGYFLKN
                     LEDFDSTFFGISPKEALLMDPQQRISLEVTWEALEDAGIPPQSLAGTNAAVYMGVNSDD
                     YSKLLLEDLPGVEAWMGIGTAYCGVPNRISYLLDLRGPSTAVDAACASSLVAIHHGRQS
                     LLTKETDVAIVGGVNALCGPGLTRVLDKAGAISKDGTCRSFDNDANGYGRGEGASVIIL
                     KRMSDALRNHDRILAVVKGSAVGQDGRTNGIMAPNGLAQEAVARSALVNVDPKTIQYVE
                     AHATSTSVGDPVEITAMSHIYGANREGNAPCYIGSVKPNVGHLEAGAGAVGFMKAVLAL
                     NKGVIPPQANLQKLNEKIDWATSGIRVPFEATSWPEVSQPRRAAICSYGYGGSVSHAVI
                     EAYQAGARDVEEADIDDDGPKILLISAPQEKRLSGYAKALGEWMASSQGTKFSVSSIAS
                     TLAVRRGHHDCRAGFLVSSHEETLDLAGRLEKGLTSPDIATGRVLGKDENNGAVWLFSG
                     HGAQWLEMGVELLAKESLFRETIQDLESVIQDEAGFSAIHALQTSDFETSDKIQVLTYV
                     MQIGLATLLRSKGARPSAVIGHSVGEIAASVITGALTPYEGALVVCRRSVLYREVMGKG
                     AMVLVNIPFAEMLTTLEARSDITASINSSPSSCVVSGSIEAINRWSAIWEERGIKIFKV
                     KSDVPFHSPLLNCLATSLYTSLVTALAPRTPFIPLYSTATENPREPVPRDASYWVRNMV
                     RPVLLTSAVEAAAKDGYRVYLEVSTHPVITHSVNETLMDMDIEDAVVIPTLLRNKPTRK
                     ALLKSLMMMWLKGVTINWKLHFGGVAWAHEVPKTEWKHQSFWKPIGTGSTDTSTMVHEV
                     TSHTLLGQRTSVAGEKITVFTSKLDNDTKPFPGNHPLHGTEIIPAAVLFNTFLHATGTK
                     SLTNVNLRVPVAISAPRDIQVLVQGTEVRLMSRLVQEGEEKEGDRLASWVTHTTASTVA
                     EPASDIPAYGADINIPSIRARIGTELKTSFSIDYLSGVGVPAMGFPWAVTRHVGNTKEM
                     LVLVDVSPETANDATLPWSATSWAPIFDAATSIGSTLFYETPRLRMPAQVGSTSVRDGA
                     VPPKKAYIYVQEASSTSTLAVDVTITNEAGMALAKFTSMRFSEIEGTPGARKDNIDGLV
                     HQLAWPVARLSEVPSLLKHVVLVGDEDHAFMEPYLRQLSKRRVKTTCVGSAQSLSVESN
                     SEGTVILYLPSRVASIDDVPEAANRFCKDLLDIVKYAAANETNTRVFAVTDEALHGTSC
                     SSLAHAPLLGLSRIIAAEQPSLWGALIDVDCETFPFHVVKNMSGADVVKVEDSIPRVGR
                     LRAMPQNKSYPSNQSAKLQPRPDGTYIISGGLGALGLEVASFLVERGARRIVLLSRRAL
                     PPRKDWNVAIEPEKGIIGNIQALEMVGATVYCLALDLSAANASVILSEKLDLLSLPPVV
                     GVVHAAGVVGDELVLSTTPTAFNRVLAPKVAGAITLHKAFPPTSVDFFMLFSSCGQLFG
                     FPGQASYASGNAFLDGLAEHRRARGDNAIALQWTSWRGLGMAATTQASADFIEAELEGK
                     GITSISRDEAFLAWDHVAKFDIAHAVILRTRTLDATEVLPADILTEIAVRRAPSTPSPV
                     ESVPAPAPALPPPGPDRTVYLTAQIAGCVATVLQLPDVSDVDPRVALPELGMDSVMTVA
                     LRKQLQMTLGVRVPPTLVWGHPTVGHLVRWFAGKV"
     aSDomain        join(1291..1443,1509..2621)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks2_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="1.90E-158"
                     /label="pks2_PKS_KS.1"
                     /locus_tag="pks2"
                     /protein_end="461"
                     /protein_start="39"
                     /score="519.7"
                     /tool="antismash"
                     /translation="IVVVGMACRVAGGNDTPEKFWESIMNKTVSSGEIPAMRWEPYYRR
                     DSRNAKILDDTTSKGYFLKNLEDFDSTFFGISPKEALLMDPQQRISLEVTWEALEDAGI
                     PPQSLAGTNAAVYMGVNSDDYSKLLLEDLPGVEAWMGIGTAYCGVPNRISYLLDLRGPS
                     TAVDAACASSLVAIHHGRQSLLTKETDVAIVGGVNALCGPGLTRVLDKAGAISKDGTCR
                     SFDNDANGYGRGEGASVIILKRMSDALRNHDRILAVVKGSAVGQDGRTNGIMAPNGLAQ
                     EAVARSALVNVDPKTIQYVEAHATSTSVGDPVEITAMSHIYGANREGNAPCYIGSVKPN
                     VGHLEAGAGAVGFMKAVLALNKGVIPPQANLQKLNEKIDWATSGIRVPFEATSWPEVSQ
                     PRRAAICSYGYGGSVSHAVIEAY"
     aSModule        1291..6605
                     /complete
                     /domains="nrpspksdomains_pks2_PKS_KS.1"
                     /domains="nrpspksdomains_pks2_PKS_AT.1"
                     /domains="nrpspksdomains_pks2_PKS_DH.1"
                     /domains="nrpspksdomains_pks2_PKS_KR.1"
                     /domains="nrpspksdomains_pks2_PKS_PP.1"
                     /iterative
                     /locus_tags="pks2"
                     /monomer_pairings="pk -> pk"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1836..1883
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks2_0001"
                     /evalue="8.50E-05"
                     /label="PKSI-KS_m3"
                     /locus_tag="pks2"
                     /protein_end="215"
                     /protein_start="199"
                     /score="14.9"
                     /tool="antismash"
                     /translation="GPSTAVDAACASSLVA"
     CDS_motif       2007..2063
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks2_0002"
                     /evalue="4.10E-04"
                     /label="PKSI-KS_m4"
                     /locus_tag="pks2"
                     /protein_end="275"
                     /protein_start="256"
                     /score="13.4"
                     /tool="antismash"
                     /translation="DGTCRSFDNDANGYGRGEG"
     CDS_motif       2250..2297
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks2_0003"
                     /evalue="2.30E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="pks2"
                     /protein_end="353"
                     /protein_start="337"
                     /score="10.6"
                     /tool="antismash"
                     /translation="YVEAHATSTSVGDPVE"
     CDS_motif       2358..2402
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks2_0004"
                     /evalue="5.90E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="pks2"
                     /protein_end="388"
                     /protein_start="373"
                     /score="9.5"
                     /tool="antismash"
                     /translation="GSVKPNVGHLEAGAG"
     CDS_motif       2949..3005
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks2_0005"
                     /evalue="1.10E-04"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="pks2"
                     /protein_end="589"
                     /protein_start="570"
                     /score="14.8"
                     /tool="antismash"
                     /translation="VWLFSGHGAQWLEMGVELL"
     aSDomain        2955..3833
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks2_PKS_AT.1"
                     /evalue="7.50E-72"
                     /label="pks2_PKS_AT.1"
                     /locus_tag="pks2"
                     /protein_end="865"
                     /protein_start="572"
                     /score="234.0"
                     /specificity="substrate consensus: pk"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="LFSGHGAQWLEMGVELLAKESLFRETIQDLESVIQDEAGFSAIHA
                     LQTSDFETSDKIQVLTYVMQIGLATLLRSKGARPSAVIGHSVGEIAASVITGALTPYEG
                     ALVVCRRSVLYREVMGKGAMVLVNIPFAEMLTTLEARSDITASINSSPSSCVVSGSIEA
                     INRWSAIWEERGIKIFKVKSDVPFHSPLLNCLATSLYTSLVTALAPRTPFIPLYSTATE
                     NPREPVPRDASYWVRNMVRPVLLTSAVEAAAKDGYRVYLEVSTHPVITHSVNETLMDMD
                     IEDAVVIPTLLR"
     CDS_motif       3180..3290
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks2_0006"
                     /evalue="3.00E-12"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="pks2"
                     /protein_end="684"
                     /protein_start="647"
                     /score="38.8"
                     /tool="antismash"
                     /translation="GARPSAVIGHSVGEIAASVITGALTPYEGALVVCRRS"
     aSDomain        4035..4412
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks2_PKS_DH.1"
                     /evalue="1.80E-12"
                     /label="pks2_PKS_DH.1"
                     /locus_tag="pks2"
                     /protein_end="1058"
                     /protein_start="932"
                     /score="39.8"
                     /tool="antismash"
                     /translation="HTLLGQRTSVAGEKITVFTSKLDNDTKPFPGNHPLHGTEIIPAAV
                     LFNTFLHATGTKSLTNVNLRVPVAISAPRDIQVLVQGTEVRLMSRLVQEGEEKEGDRLA
                     SWVTHTTASTVAEPASDIPAYG"
     CDS_motif       5040..5066
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks2_0007"
                     /evalue="9.90E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="pks2"
                     /protein_end="1276"
                     /protein_start="1267"
                     /score="-3.4"
                     /tool="antismash"
                     /translation="VKTTCVGSA"
     aSDomain        5514..6089
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks2_PKS_KR.1"
                     /evalue="5.80E-53"
                     /label="pks2_PKS_KR.1"
                     /locus_tag="pks2"
                     /protein_end="1617"
                     /protein_start="1425"
                     /score="171.4"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="GTYIISGGLGALGLEVASFLVERGARRIVLLSRRALPPRKDWNVA
                     IEPEKGIIGNIQALEMVGATVYCLALDLSAANASVILSEKLDLLSLPPVVGVVHAAGVV
                     GDELVLSTTPTAFNRVLAPKVAGAITLHKAFPPTSVDFFMLFSSCGQLFGFPGQASYAS
                     GNAFLDGLAEHRRARGDNAIALQWTSWRG"
     CDS_motif       5517..5615
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks2_0008"
                     /evalue="1.30E-15"
                     /label="PKSI-KR_m1"
                     /locus_tag="pks2"
                     /protein_end="1459"
                     /protein_start="1426"
                     /score="49.9"
                     /tool="antismash"
                     /translation="TYIISGGLGALGLEVASFLVERGARRIVLLSRR"
     CDS_motif       5931..6041
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pks2_0009"
                     /evalue="7.50E-20"
                     /label="PKSI-KR_m4"
                     /locus_tag="pks2"
                     /protein_end="1601"
                     /protein_start="1564"
                     /score="63.5"
                     /tool="antismash"
                     /translation="VDFFMLFSSCGQLFGFPGQASYASGNAFLDGLAEHRR"
     aSDomain        6363..6605
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pks2_PKS_PP.1"
                     /evalue="3.40E-20"
                     /label="pks2_PKS_PP.1"
                     /locus_tag="pks2"
                     /protein_end="1789"
                     /protein_start="1708"
                     /score="64.1"
                     /tool="antismash"
                     /translation="PPGPDRTVYLTAQIAGCVATVLQLPDVSDVDPRVALPELGMDSVM
                     TVALRKQLQMTLGVRVPPTLVWGHPTVGHLVRWFAG"
     3'UTR           6615..6858
                     /gene="pks2"
ORIGIN
        1 cggtgcagct tcgtagactt ggacggggac atgctgatgc cggagtgcaa tcgcaagtgc
       61 gatacccccg attccaccgc cgactatggc gatggtaaat ggcttttgag tggagttttc
      121 ggtcgacatt tctcttgata cgatgtgtaa atgcggtgat ttgaatctgc gaggattgag
      181 aagtctgatg tatttcgagt gattatgcgg actttgacaa tgagcagtcg agaagcaata
      241 tcaaaaagct aagaatgtga tgagatcaac agattggcac aagaaaattg atacgtagag
      301 tgtagaaatg gtcttgccac cgagtggtgg tagatgacag cagaagtttc agagctccta
      361 gaatcggggc tgaggagcac atttcttcgg cctttcagtt catgtgagaa gtgagctagc
      421 tgaagcatag cgagacaaaa ctgaggctgg ctgttcaaac ttggcaaatg cagcatttct
      481 gcaagtctcc gggtcacgat agcactgccg taagcctaca ctctcacatc ctttttatag
      541 acacatgggc tgctaatatg cccaacacag ccctacgcct atctttcatg gctcttggtc
      601 atgtccttct tcgagctttt cgaggaatta ggtgggcctt gattcatact cctccatagc
      661 ggcagagatg ttcggccgca gccgtggtgg tcagaacagg cagtcatgtt tcggtgcgtt
      721 cgagctatct atgcctttca gcttgacacg agacaatctt ggcgaaggtt gtttgctatt
      781 ccctctcgtc cacgtgtttg tgagaatgag atatggagta tctataagaa tgaaggtaaa
      841 agctgcaagt atgttgtaga tcagatcctc gcaaacatct ttctccttca acttgagcaa
      901 gtacctgctc gaaatactcg aacctctagc gtccaggcta catttcagga actcaatcca
      961 ttctttctag aaacttactc ctgggctttt gtctttaatt cccccataga tccttctctt
     1021 ttgtgttctc tatttgaacc atctagtctg tctctcaatc accatgtctc cctttcttga
     1081 aggtattacc acaatccgcc ttgatttttt ccctatactg ataaatttag tagctgagcc
     1141 tacggaacat gagtcctctg atatgtccgt gctttctaaa tggaccgcct ctagctctcc
     1201 ctcagtatct ggaaaggtca gtcgagccac tcccctgccg atgattttac atttgctaac
     1261 ttggtaacac tacagagcga gattgatgaa atagttgttg ttggtatggc atgccgcgtg
     1321 gccggaggaa atgatacccc tgaaaagttc tgggagtcca tcatgaacaa gactgtctcg
     1381 tctggagaga tcccagctat gcgatgggaa ccatactacc gccgtgactc tcggaatgct
     1441 aaggtatgtc gtcatctatc ccctctttgt ctctcgtttc gtgtttctca ctgactttta
     1501 ctcccaagat cctcgatgac acaacaagca aggggtattt tctgaagaat ctcgaagatt
     1561 tcgacagcac attctttgga atttctccca aggaagcgct actgatggac cctcagcaga
     1621 ggatctctct ggaagtgaca tgggaagcac tggaagacgc aggaatccca ccccagagtc
     1681 tcgccggtac caatgcggca gtatatatgg gcgtcaactc cgacgattat tcgaaactcc
     1741 ttctcgaaga tctcccaggc gtggaagcct ggatgggaat tggaacagcc tactgcggcg
     1801 tgccgaaccg aatttcgtac ttgctcgacc tcagaggtcc ttcaactgca gtcgatgcgg
     1861 cctgtgcttc atccttggtt gccattcatc acggtagaca atcgctcttg acgaaggaga
     1921 ccgatgttgc cattgtagga ggagtcaatg cactttgtgg ccctggtctg acccgtgttc
     1981 tcgacaaagc cggagcgatc tctaaggatg ggacatgtag atcgttcgac aatgacgcga
     2041 acggctacgg tcgtggtgag ggagcgtcgg ttattattct gaagcgcatg tcagatgcac
     2101 ttcgtaatca cgacaggatt ttggcagtcg tcaagggctc cgctgttggt caggatggcc
     2161 gtaccaatgg cattatggca cctaacggac ttgctcagga ggcggttgct cggtccgcac
     2221 tagtcaatgt cgatccaaag accattcaat acgtcgaagc gcatgcgacg tccacatctg
     2281 ttggtgaccc cgtcgaaatc accgccatgt cacacatata cggcgccaac agagagggaa
     2341 atgctccttg ctacatcgga agcgtgaagc ccaacgtcgg ccacctagaa gctggtgcag
     2401 gtgcggtagg tttcatgaag gcagtcttag cactcaacaa aggtgttatt cccccgcaag
     2461 caaatcttca aaagctgaac gaaaaaattg actgggccac ttccggtatc cgagtcccat
     2521 tcgaagctac cagctggcca gaggtttccc aaccacgaag agcggccata tgctcttatg
     2581 gatatggtgg cagcgtatct cacgcggtca ttgaagcata tcaagcaggc gctcgtgatg
     2641 ttgaggaggc ggatattgat gacgacggcc ccaagatctt gctcatatcg gcaccgcagg
     2701 aaaagcgact ttcaggatat gcaaaagctc tgggtgaatg gatggccagc agccaaggca
     2761 ccaaattctc agtttcctcc attgcgtcca ctctcgcggt tcgacgaggt catcatgact
     2821 gtagggctgg atttcttgtc agcagccatg aagagacgct cgatctggct ggaagattag
     2881 agaaaggatt gacatctcct gatatcgcca ctggccgagt gcttggcaag gatgagaaca
     2941 atggagctgt ttggctcttc tcaggacacg gtgcgcaatg gctggaaatg ggcgtggaat
     3001 tgttggcaaa ggaatcactc ttccgtgaga ccattcaaga cctcgaatca gtcattcaag
     3061 atgaagctgg gttttcagcc attcatgcac tgcagactag tgactttgag acctcggata
     3121 agatccaggt tctcacctat gtcatgcaga ttggtcttgc aactctcctt aggtccaaag
     3181 gtgcccgtcc tagtgcggtg attgggcatt ctgttggtga gattgcagca tcggttatta
     3241 cgggggcttt gactccctac gaaggcgctt tggttgtttg cagacggtct gtcctttacc
     3301 gcgaagtaat gggcaagggg gccatggttc tcgtcaatat tccctttgcc gagatgctaa
     3361 cgacacttga agcccgttcg gatatcacgg cttccatcaa ctcatcgccg tcatcctgtg
     3421 tcgtctctgg ttcgattgag gcaatcaata ggtggtctgc catctgggaa gagcgtggaa
     3481 tcaaaatatt caaggtcaag tcggacgtgc cattccacag cccgcttctc aattgtctcg
     3541 cgacatctct atatacatca ctcgttacgg ctcttgcacc aaggactcca ttcattcccc
     3601 tctattcaac agctaccgaa aatcctcgtg aaccggttcc tcgcgatgct agctactggg
     3661 tccgtaacat ggtgcggcca gttttgctca ccagcgccgt cgaggctgca gcaaaggatg
     3721 gatacagagt ctatttggaa gtctccactc acccggtcat cacacattcg gtcaatgaga
     3781 ccctgatgga tatggatatc gaggacgctg ttgttattcc aacacttctg cgtaacaaac
     3841 ctacccgaaa ggctctcctc aagagtttga tgatgatgtg gttgaaaggt gtgacgataa
     3901 attggaagct gcacttcgga ggcgtagctt gggcccatga agtcccaaag acggaatgga
     3961 aacatcagag cttctggaaa cccattggca cgggatctac cgatacaagc acgatggttc
     4021 atgaagttac ttctcacact cttctcggtc aacgcacgtc tgttgctgga gaaaaaatca
     4081 ccgtcttcac ttcgaagctt gataacgaca caaagccatt cccgggaaat cacccgctcc
     4141 atggaacgga gatcatacca gcagctgtac tgtttaatac cttcttgcac gcaactggga
     4201 ccaagtcgct gacgaatgtc aaccttcgag ttccagtcgc catcagcgca ccgcgggaca
     4261 tccaagttct cgtacaagga actgaagtac gccttatgtc tcgccttgtc caagagggtg
     4321 aagagaaaga aggcgatcgc ttggcgtcgt gggttaccca caccaccgca agcaccgttg
     4381 ctgaaccagc tagcgacata ccagcctacg gggctgatat caatattcct agcattcggg
     4441 ctcgcatcgg aactgagctg aagaccagct tctctattga ttacctttct ggtgtgggtg
     4501 ttcccgcaat ggggttccct tgggcagtca cccgccatgt tggaaatacc aaggagatgc
     4561 ttgtacttgt agatgtctct ccagaaacag caaacgatgc gactctccca tggagtgcca
     4621 catcttgggc tccaattttc gatgctgcga cttccattgg ttccacgctc ttctacgaga
     4681 caccacgttt gcggatgcct gctcaggttg gtagcacaag tgtgcgtgac ggtgcagttc
     4741 ccccaaagaa agcctatatc tatgtgcaag aagctagcag tacctctaca cttgctgtcg
     4801 atgtcacgat tacgaatgaa gccggcatgg cacttgctaa gtttacctcg atgcgctttt
     4861 cggaaattga aggtaccccc ggtgccagaa aggacaacat agatggcctt gttcaccaac
     4921 tcgcttggcc agtcgctcgt ctctctgaag ttccttcgct tttgaaacat gtcgtacttg
     4981 ttggcgatga ggatcatgct ttcatggagc catatctccg acaactctcg aagcgccgcg
     5041 ttaagactac ctgtgttggt tcagcacagt cattgtcagt tgaaagcaat tcggaaggca
     5101 ctgtcatctt gtatctccca agtagggtag catcaatcga tgatgtaccc gaggcagcca
     5161 acaggttttg caaggatctt ctcgacattg tgaaatatgc tgctgcaaac gagacgaaca
     5221 ctcgtgtatt cgcagtcaca gatgaagctc ttcatggtac cagctgcagt tctctcgcac
     5281 atgctccatt gttaggacta agtcgcatca tcgcagctga gcagcctagt ctctggggcg
     5341 cgctcattga tgtggactgt gaaacctttc ctttccacgt cgtcaaaaac atgtccggag
     5401 cagatgtggt caaggtagaa gactccattc ctcgagttgg tcgcctgaga gccatgccac
     5461 agaataaatc gtatccctca aaccagtctg caaagcttca gcctcggcct gatgggactt
     5521 atatcatctc tggcggtctc ggcgctcttg gtctcgaagt cgcttctttt ttggtggaac
     5581 gcggagcacg cagaatcgtc ctcctctctc gtcgtgccct cccacctcgc aaagattgga
     5641 atgtcgcgat agaaccggag aagggaatta ttggaaatat ccaagcactt gaaatggtcg
     5701 gagcaacggt atattgtctc gctctcgacc tgagtgcagc caacgcctcc gtcattcttt
     5761 ctgaaaagct agacttgtta tccttaccgc ccgttgtggg agttgtacac gccgccggcg
     5821 tcgtaggaga tgagctggtt ctctccacca ccccaactgc cttcaatcgc gttctcgcac
     5881 ccaaggtagc cggggccata actctgcaca aagcattccc acccacctca gtcgacttct
     5941 tcatgctctt ctcttcctgc ggccaactct tcgggttccc aggccaagct tcctacgcaa
     6001 gcggcaacgc cttcctagac ggtctcgcgg aacaccgtcg cgcacgcggc gacaacgcaa
     6061 tcgccttgca gtggaccagc tggcgcggtc taggcatggc agctaccacg caagccagcg
     6121 cagatttcat cgaagccgag ctcgaaggca aaggaatcac cagcatcagc cgcgacgagg
     6181 ctttcctcgc ctgggaccac gtcgccaaat tcgacatcgc ccacgccgtc atcctccgca
     6241 cccgcaccct cgacgcaacc gaagtccttc ccgcagacat cctgaccgaa atcgccgtcc
     6301 gtcgcgcacc atccacgccc tcccccgtcg aatctgttcc tgctcctgcc ccagcgctcc
     6361 cgccgccagg gccagaccgc accgtctacc tcacggccca gatcgccggg tgcgtcgcta
     6421 ccgtcctgca gctccctgat gtcagcgacg tggatccgag ggttgcgctg ccggagctgg
     6481 gtatggattc ggtcatgacg gtcgcgctgc ggaaacagtt gcagatgacg ttgggggtga
     6541 gggtgccgcc tacgttggtg tgggggcatc cgacggtggg gcatttggtg aggtggtttg
     6601 ctgggaaggt gtgagtgtgg gtttgtgagg gtgttggggg gtaaggagtc gatttgggta
     6661 gtgaggaatt gatttgggga tgagttgtgt tttgtgtttg ttgcttctgc gatggcgtta
     6721 tgtttttggg gtttgatagg tttcatgtgc gtggagttta ggtatttttg aatgagagaa
     6781 gtttttggaa aggagtcagt ggtatgatat ttagtctcga tgtttctcct agattggtaa
     6841 aaaaaaatgc ctagatgagt ttaatctgag tgatatgtga tgtgatcatt gcgagtgagt
     6901 tctcgcttaa taagtagaag accgccgagt ccgggatttt ggagggtggg gagggacagg
     6961 tgatgggttt atttagagga gagggtggga tggcgattgg ggtattatgt tcttcaagct
     7021 gattaaggca tttgtcgaaa atagcttcga aggtttggtg tgatgcgttt taggggttaa
     7081 tcgcgttatc tctatctttg aggttgatga ctacgcgaat ggcttggtga gatgtaggtg
     7141 tctctacacc taacgctttt tcaagtaagg aaaagaagta tagtaagtct ctggtactag
     7201 gactcgttta gatgctgata aatcattttt tagattggta caaaagacat gaggtctgga
     7261 tcttctgtga tatctggctg aagtatatag cgccaggggg tggaatatgg aatgtgcaat
     7321 atcagtagtc ctaccgtttc gacttgtgcg attcacactt aacgtatctt acgcgaggtg
     7381 agttctatct tttgtataag aacttgggag ttgtgtgtaa cataccccgc atttaggctc
     7441 gtgtaaccta tatgtctcgt gcaaacggac catgctcact tttgacgatt aacgcactcc
     7501 caaaccgaaa taagaattag cacataggga tactgaaccg ttgagacaaa ccatcgattt
     7561 aagtctcaca acatctaacc ccaccgtccc ctccacaaat cccttcgcac aaaatcaaca
     7621 acacagctac cttcaaaccc acctccctcg tttccaaaca tggccgaatc aagcacttca
     7681 aaccaagaga aatcccaata ccaccactac atcccccgat tcatcctccg caactacgcc
     7741 catccagcct cccccccaaa aaccacctca aaatccaaaa agcgcggcaa gcgaaaatcc
     7801 acagcacggc aaccacccct gatgctttct agcatcgatc tctcgggcga ggtcgctcag
     7861 atcaaagaat caacagtcga taagacattc gggataacag acatgtatcg agatttcagc
     7921 cgctctacca agcaacatgc gattgaggaa caactttccg tgctcgaatc tcgtgcggga
     7981 gaggtgataa gcgggattcg cagggcgttt gaagcagggc gagcaagcgc tgagataaaa
     8041 cgcccgcaga gggatgtgtt gcggaagttt ttgttcatca tgaaataccg ggggaggagg
     8101 tttcacaaac ggtatttcca cgaaaccgcc gaggagtatg atgaagatga taaggagcgc
     8161 atgttagcgt atatgaccca aagagggttt aagaagccga ttgacgtctg gttcgacaac
     8221 atcaagggga ttctggagtt gaagatggat tatgagatga actggatgga gacgatccaa
     8281 gagaggatct atccggatga tgcgaaatgg tttatcaatc atacgcagca gttttatatg
     8341 gcgttgtgta cgccggcagc cggggacgag gagtttctgc tcagtcagaa tgcgtatggg
     8401 gttcatgagg gggcgtcgag tagtggggtt gatccgggat ccggggaagt tataccaggt
     8461 gtatataccg agtttcatgt cttcgcgccg atcgctccga gacttgttat tgtgctgagg
     8521 acgttcttgc ttcctgttcc ggaggaggat acggatccgg acatgaaggc gttgagggag
     8581 ggctggtatg aggcggttgt ctcacagcac ctacatcctg agagtgtggg ctcggttctt
     8641 agggatctgc cgattatgaa agcgcggaac tcgtatacga agattgttga tgggagttat
     8701 gtgacggtga atggcggccc aaagggtgtg agcgatgtgt tttactttcc gttttgcgct
     8761 gttgagacgg ggtttgtgga taagattaat gggatcatgt tgaatgagag tcatcggatt
     8821 gatgggattg tgtttgctaa taaggcagct gcgtgcaaga ctatcaggag ttacgtgaat
     8881 gattgcacgg atttcggtct tgatgaggag cgctttcaga atgttaagaa attgcgacaa
     8941 gcgcttaagt tgctagattc aagttcatca gaccagactg cgagcggtca acccgaattt
     9001 gacgcaattg gtgtggaggc acttattgga tcgtctgata aggtgtttga actattcaag
     9061 caaaacaaaa aagcagcgga attgtattcc gaaataagta agtcctccaa ctccagatcc
     9121 gtcacctcct aacacatcca gccaacaatt caacactcga ccgcaaagac ttcactcaag
     9181 cacgaggact actcttcaca cgcatcaaac tcgacacccg cacccgcggc ctccctccag
     9241 caaagcgaga ggaagctcga accaaattcc tcgaagtcta cgacactgtc ccattccaag
     9301 tcatatggat ctatctcaaa cggatccgct ttatgaaaca cggcggtacg atgataatgc
     9361 cggagaatgt tttggaggat actgagttgg atgttggctt gctttttggg atggaagatg
     9421 ttgttgcgag gggtatgtgt tgtgcttctc aatgacgagg gtcagggatg ctgatgattt
     9481 tatagcgaag gattgttttg aggatgggaa ggcgacgagg gttatgcatt atgcgtcgtt
     9541 caatgaggcg ttgattgcga agccggattg gggactaatt gagatattga ctttggacga
     9601 tgatgggatg agaaggttgc atgaggagaa ggagttggtg ttgggcgctc aaggtaacca
     9661 agaagaaccc atttgttaac tatccactaa cagctgtttt ttacaggtag tatttgcgat
     9721 tgcggtaagt cctaccaaaa tcctggtttc cccaatgctg aaattatcca ggtatccccg
     9781 tattggaagg catcgcgtca gcagcacggg ttatgactac aaccgaacaa cttcataacg
     9841 caagacaatc tccattcgac actatattca cgaaagatga gaggatccga acaacttcat
     9901 aacgcaagac aatctccatt cgacactata ttcacgaaag atgagaggat c
//
