LOCUS       BGC0001284              5292 bp    DNA     linear   PLN 18-AUG-2015
DEFINITION  Phaeosphaeria nodorum SN15 polyketide synthase mRNA, complete cds.
ACCESSION   BGC0001284
VERSION     BGC0001284
KEYWORDS    .
SOURCE      Parastagonospora nodorum SN15
  ORGANISM  Parastagonospora nodorum SN15
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae;
            Phaeosphaeriaceae; Parastagonospora.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 00:22:55
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /db_xref="taxon:321614"
                     /mol_type="mRNA"
                     /organism="Parastagonospora nodorum SN15"
                     /strain="SN15"
     protocluster    1..5292
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[0:5292]"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..5292
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..5292
                     /SMILES="CC(=O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..5292
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001284"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5292
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             1..5292
                     /EC_number="2.3.1.-"
                     /NRPS_PKS="Domain: SAT (19-261). E-value: 3.2e-48. Score:
                     156.2. Matches aSDomain: nrpspksdomains_AKN45693.1_SAT.1"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (392-823). E-value:
                     3.2e-148. Score: 486.0. Matches aSDomain:
                     nrpspksdomains_AKN45693.1_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (923-1220). E-value: 6.7e-76.
                     Score: 247.3. Matches aSDomain:
                     nrpspksdomains_AKN45693.1_PKS_AT.1"
                     /NRPS_PKS="Domain: PT (1302-1613). E-value: 3.3e-92. Score:
                     301.0. Matches aSDomain: nrpspksdomains_AKN45693.1_PT.1"
                     /NRPS_PKS="Domain: ACP (1699-1762). E-value: 1.1e-12.
                     Score: 40.0. Matches aSDomain:
                     nrpspksdomains_AKN45693.1_ACP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     itr_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 72.4; E-value:
                     4.9e-22)"
                     /gene_kind="biosynthetic"
                     /note="produces alternariol; similar to Alternaria
                     alternata PksI and Penicillium aethiopicum gsfA; SnPKS19"
                     /product="polyketide synthase"
                     /protein_id="AKN45693.1"
                     /sec_met_domain="PP-binding (E-value: 7.8e-13, bitscore:
                     35.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.2e-77, bitscore: 247.3,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="itr_KS (E-value: 4.3e-162, bitscore:
                     525.7, seeds: 96, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MTRAKVIYFSGEIPQGDPEGDQRNLFRKLHLLSKERDYPILASFL
                     ETVTWAMKEEHRRLVRAQRDLLPTFESILDLTDHVVELRKTSLGGAIERVLVLAFQLGS
                     FIAYHEAHPLEYNFQASDTFLIARGPGMLSAAAVALCPSLPMISSISADITRVAFRFGL
                     VVDQVCRSLEVSPDEINSAGAWIYCVYGVDPQKAHEAVTRFNAEKAYAETSMASVFNDD
                     GKSVSIGGPPSTLKTLFTECDFFKRTKNVPMKKVQGMWHTGKVYGTEHVHQIIPKIHQK
                     HQLYLPLFSPVKGQPLEAASATDLLEQIMEEMLTQRIRWDRTIQNVTERLKQASPESTQ
                     LIAIQPSQYVESLIGQWRADMPTTTHTTEDMMSAVMDLPLGNSRAKDAKSSKIAVVGMA
                     CRFPGGADNAEKFWELLAQGRDVHSPIPSDRFNIETHVDPAGKVPNTSKTPYGCFVDNP
                     GLFDAMFFGMSPREAEQTDPMQRLALVTAYEALEHSGYVHGRGIHARRVGTFYGQASDD
                     YREVNSGQDVGTYFIPGGCRAFGPGRINYFFKFWGPSFSVDTACSSSLAAIQAACTSLW
                     SGDVDMAITGGMNIITNSDVYAGLSNGHFLSPTGGCKTWDEGADGYCRADGVGSVVLKR
                     LEDAEADNDNILGVVLAAATDHSAEAVSITHPHDVAQAHLYNQVARRAGIDPLAVGYVE
                     MHGTGTQAGDSNEMKSVTNVFAPSTGHIRDRDSPLHIGTVKSNMGHGEAAAGIMAFVKT
                     MLVFQKGIIPPHIGIKTRFNPALPEDLAKRNVVIPVTAAIWVRNSDRKRLAMVNNFGAA
                     GGNTSIIIEEAAPRPRTSEDVRKAQVITVSAKTANSLQENLKALVDYIEARSELSVADL
                     AYTLSARRNHYNYRVSVLATSTAEAASLLRPHIKTSLSQTPHSGKQVPIAFAFTGQGTF
                     YIGIGAQLYRDSHEFRKHIDQLDSLVRRQNFASFLPVVSGNVQPEDVSIVTMNLAIVCV
                     EIALARLWESFGIKPSMVIGHSLGEYAALAVAGVLSDSAAIFLVGTRARLLTSKCTSRT
                     HGMLSVRASAADIKHAGGDIPFEVSCINGPNETVLGGTLSNMEALSATLAAAGYRTFKL
                     DLPHAYHTYQMDTIVDELVKQTETVVCKKTTIPIISPRFSRVMTSEDSIDVSYLIGATR
                     ETVDFAGALDDAWQSGLVNESTIWLEMGHHPTCSGFISRTLSSTRMALPSLQRDTDNWL
                     TLAKTLCSLYSAGIPIDWNEYHRPFEQALRLIDAPTYAWTNKNYWIQYRGDWNLTKGRA
                     MPKLDPTTTVVPVSKRFQTSSIHRLISEQYTDGKAILSAESSITDPSLQGVVDGHAMNG
                     YGVASSFLHAEMAFTLAKRVSDKSFASAVSFGINVADFEYHEPVVKLINTSEPQPILVS
                     AEADLEKMEVHVKWFNPAKEIWYCHATVFYEDPSSWLSTWSRSTKLITSRIDALNDMAV
                     TGKASKLTTDLAYSLFGKLVGYSKLYQTMQSVILNEDEAMAEVQFPEDTGGSWTVPPHF
                     IDGLISLSGFILNGGTHFDNTNNFFITPSWKSMRFARPLTPGGRYTAYVRMVPSDNHSF
                     VGDVYVLQGSEIVGVVEAILFLQWPRVMLNRFFRPADVTAKPAAKVPGKSEPSTRPHFK
                     PHHVSRHKPTLTPRSPDEGSENSDSSGVIISRPGGYSSSDQDMEELPSPPAGMNDDMEK
                     ALALVAEELAVDIGLLTDDALIADLGLDSLMSLVMSQRLREELGLEIRDAFFLEITTIQ
                     DLKALLR"
     aSDomain        58..783
                     /aSDomain="SAT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AKN45693.1_SAT.1"
                     /evalue="3.20E-48"
                     /label="AKN45693.1_SAT.1"
                     /locus_tag="AKN45693.1"
                     /protein_end="261"
                     /protein_start="19"
                     /score="156.2"
                     /tool="antismash"
                     /translation="GDQRNLFRKLHLLSKERDYPILASFLETVTWAMKEEHRRLVRAQR
                     DLLPTFESILDLTDHVVELRKTSLGGAIERVLVLAFQLGSFIAYHEAHPLEYNFQASDT
                     FLIARGPGMLSAAAVALCPSLPMISSISADITRVAFRFGLVVDQVCRSLEVSPDEINSA
                     GAWIYCVYGVDPQKAHEAVTRFNAEKAYAETSMASVFNDDGKSVSIGGPPSTLKTLFTE
                     CDFFKRTKNVPMKKVQGMWH"
     aSDomain        1177..2469
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AKN45693.1_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="3.20E-148"
                     /label="AKN45693.1_PKS_KS.1"
                     /locus_tag="AKN45693.1"
                     /protein_end="823"
                     /protein_start="392"
                     /score="486.0"
                     /tool="antismash"
                     /translation="IAVVGMACRFPGGADNAEKFWELLAQGRDVHSPIPSDRFNIETHV
                     DPAGKVPNTSKTPYGCFVDNPGLFDAMFFGMSPREAEQTDPMQRLALVTAYEALEHSGY
                     VHGRGIHARRVGTFYGQASDDYREVNSGQDVGTYFIPGGCRAFGPGRINYFFKFWGPSF
                     SVDTACSSSLAAIQAACTSLWSGDVDMAITGGMNIITNSDVYAGLSNGHFLSPTGGCKT
                     WDEGADGYCRADGVGSVVLKRLEDAEADNDNILGVVLAAATDHSAEAVSITHPHDVAQA
                     HLYNQVARRAGIDPLAVGYVEMHGTGTQAGDSNEMKSVTNVFAPSTGHIRDRDSPLHIG
                     TVKSNMGHGEAAAGIMAFVKTMLVFQKGIIPPHIGIKTRFNPALPEDLAKRNVVIPVTA
                     AIWVRNSDRKRLAMVNNFGAAGGNTSIIIEEA"
     aSModule        1177..5286
                     /complete
                     /domains="nrpspksdomains_AKN45693.1_PKS_KS.1"
                     /domains="nrpspksdomains_AKN45693.1_PKS_AT.1"
                     /domains="nrpspksdomains_AKN45693.1_PT.1"
                     /domains="nrpspksdomains_AKN45693.1_ACP.1"
                     /iterative
                     /locus_tags="AKN45693.1"
                     /monomer_pairings="mal -> mal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1480..1503
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AKN45693.1_0001"
                     /evalue="9.90E+01"
                     /label="PKSI-KS_m4"
                     /locus_tag="AKN45693.1"
                     /protein_end="501"
                     /protein_start="493"
                     /score="-2.9"
                     /tool="antismash"
                     /translation="SGYVHGRG"
     CDS_motif       1654..1701
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AKN45693.1_0002"
                     /evalue="2.00E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="AKN45693.1"
                     /protein_end="567"
                     /protein_start="551"
                     /score="19.7"
                     /tool="antismash"
                     /translation="GPSFSVDTACSSSLAA"
     CDS_motif       1843..1881
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AKN45693.1_0003"
                     /evalue="3.60E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="AKN45693.1"
                     /protein_end="627"
                     /protein_start="614"
                     /score="10.7"
                     /tool="antismash"
                     /translation="WDEGADGYCRADG"
     CDS_motif       2074..2121
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AKN45693.1_0004"
                     /evalue="9.20E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="AKN45693.1"
                     /protein_end="707"
                     /protein_start="691"
                     /score="11.6"
                     /tool="antismash"
                     /translation="YVEMHGTGTQAGDSNE"
     CDS_motif       2194..2238
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AKN45693.1_0005"
                     /evalue="8.20E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="AKN45693.1"
                     /protein_end="746"
                     /protein_start="731"
                     /score="9.1"
                     /tool="antismash"
                     /translation="GTVKSNMGHGEAAAG"
     CDS_motif       2770..2817
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AKN45693.1_0006"
                     /evalue="4.70E-03"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="AKN45693.1"
                     /protein_end="939"
                     /protein_start="923"
                     /score="10.1"
                     /tool="antismash"
                     /translation="FTGQGTFYIGIGAQLY"
     aSDomain        2770..3660
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AKN45693.1_PKS_AT.1"
                     /evalue="6.70E-76"
                     /label="AKN45693.1_PKS_AT.1"
                     /locus_tag="AKN45693.1"
                     /protein_end="1220"
                     /protein_start="923"
                     /score="247.3"
                     /specificity="consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="FTGQGTFYIGIGAQLYRDSHEFRKHIDQLDSLVRRQNFASFLPVV
                     SGNVQPEDVSIVTMNLAIVCVEIALARLWESFGIKPSMVIGHSLGEYAALAVAGVLSDS
                     AAIFLVGTRARLLTSKCTSRTHGMLSVRASAADIKHAGGDIPFEVSCINGPNETVLGGT
                     LSNMEALSATLAAAGYRTFKLDLPHAYHTYQMDTIVDELVKQTETVVCKKTTIPIISPR
                     FSRVMTSEDSIDVSYLIGATRETVDFAGALDDAWQSGLVNESTIWLEMGHHPTCSGFIS
                     RTLSSTRMALPSLQRD"
     CDS_motif       3001..3120
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AKN45693.1_0007"
                     /evalue="1.10E-15"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="AKN45693.1"
                     /protein_end="1040"
                     /protein_start="1000"
                     /score="49.6"
                     /tool="antismash"
                     /translation="GIKPSMVIGHSLGEYAALAVAGVLSDSAAIFLVGTRARLL"
     aSDomain        3907..4839
                     /aSDomain="PT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AKN45693.1_PT.1"
                     /evalue="3.30E-92"
                     /label="AKN45693.1_PT.1"
                     /locus_tag="AKN45693.1"
                     /protein_end="1613"
                     /protein_start="1302"
                     /score="301.0"
                     /tool="antismash"
                     /translation="TSSIHRLISEQYTDGKAILSAESSITDPSLQGVVDGHAMNGYGVA
                     SSFLHAEMAFTLAKRVSDKSFASAVSFGINVADFEYHEPVVKLINTSEPQPILVSAEAD
                     LEKMEVHVKWFNPAKEIWYCHATVFYEDPSSWLSTWSRSTKLITSRIDALNDMAVTGKA
                     SKLTTDLAYSLFGKLVGYSKLYQTMQSVILNEDEAMAEVQFPEDTGGSWTVPPHFIDGL
                     ISLSGFILNGGTHFDNTNNFFITPSWKSMRFARPLTPGGRYTAYVRMVPSDNHSFVGDV
                     YVLQGSEIVGVVEAILFLQWPRVMLNRFFR"
     aSDomain        5098..5286
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AKN45693.1_ACP.1"
                     /evalue="1.10E-12"
                     /label="AKN45693.1_ACP.1"
                     /locus_tag="AKN45693.1"
                     /protein_end="1762"
                     /protein_start="1699"
                     /score="40.0"
                     /tool="antismash"
                     /translation="ALVAEELAVDIGLLTDDALIADLGLDSLMSLVMSQRLREELGLEI
                     RDAFFLEITTIQDLKALL"
ORIGIN
        1 atgacacggg ccaaagttat ctatttctct ggggaaatcc cccaaggaga cccagagggt
       61 gaccagcgta accttttcag gaaattacac ctacttagca aggagaggga ctatccaatt
      121 ctagcctcct ttcttgaaac agtgacgtgg gctatgaaag aggaacatcg tcgattggtc
      181 cgggcccaaa gggatttatt accaaccttc gagagcatct tggacttgac cgatcatgta
      241 gtcgagctca gaaaaacgtc tcttggtgga gcgattgagc gagtgctggt ccttgctttt
      301 cagcttggaa gctttattgc ataccacgaa gctcatcctc tcgaatacaa cttccaggcg
      361 tctgatacct tcctcattgc gagaggccca ggcatgctat cagcggcagc tgtcgcactc
      421 tgtccaagtt tgccaatgat ttcaagcatt tctgcagaca tcacaagggt agcgttccga
      481 tttggactag ttgttgacca agtctgccga tcacttgagg tttcgccgga tgaaataaac
      541 tccgctggag cttggatata ttgtgtatac ggtgttgatc cacaaaaagc ccatgaagcg
      601 gttacccgtt tcaacgcaga gaaggcatac gctgagacga gcatggcatc tgtcttcaat
      661 gacgatggaa aatctgtcag cattggtggc ccaccgtcga ccctcaagac actcttcaca
      721 gaatgcgatt tcttcaaacg taccaagaat gtacccatga agaaagtcca aggcatgtgg
      781 catacgggta aggtctatgg tacggaacat gttcaccaga tcatcccaaa gatccatcag
      841 aagcatcagt tgtatcttcc actattctcc cctgtgaagg gacaaccgtt ggaagcagca
      901 agcgccacag atcttctcga gcagatcatg gaggagatgc taacgcaaag aattcggtgg
      961 gatcgaacga ttcagaacgt tacagaacgg ttgaagcaag cctcgccaga gtcgacccag
     1021 ttgatcgcca tccaaccatc acaatatgtt gaaagcttaa ttggacagtg gagggctgac
     1081 atgcccacta ccacacatac aaccgaagac atgatgtcag cagtcatgga ccttcctctc
     1141 ggaaacagcc gtgctaagga tgcaaagtca tccaaaatcg ccgtcgtggg tatggcatgc
     1201 cgcttcccag gaggtgccga taacgcagaa aagttctggg agttattggc tcaaggtcga
     1261 gacgtccaca gtcccattcc ctcggatcgc ttcaacattg agactcacgt tgacccagcg
     1321 gggaaagttc caaacacgtc caaaactcct tacggttgtt tcgtagacaa tcctggtctg
     1381 tttgacgcca tgtttttcgg catgtctccc cgagaggcag aacagaccga tccgatgcag
     1441 agactggccc tcgttaccgc ctatgaggcg ttggagcact caggatacgt gcatggtcgc
     1501 ggtatccacg cgcgacgcgt gggcaccttc tacggacaag ctagtgacga ctaccgcgag
     1561 gtgaactccg gacaagacgt cggtacttac ttcattcccg gagggtgtag agccttcggt
     1621 cctggccgca tcaactactt cttcaaattt tggggtccga gtttcagtgt cgacacagca
     1681 tgttcttcca gtcttgcggc gatacaagct gcatgcacct ccctatggag tggagatgtc
     1741 gatatggcaa tcactggggg catgaacatc attaccaact ctgatgtata tgctggcctc
     1801 agcaacggcc attttctctc cccgactggg ggctgcaaga cgtgggatga gggagctgat
     1861 ggatattgtc gcgccgatgg tgtgggtagc gtggtcttga aaagactaga agatgctgag
     1921 gctgataatg acaacatcct tggagttgtg ctcgctgcag ccactgatca ttctgctgag
     1981 gcagtgtcga ttacacaccc ccacgatgta gcacaggctc atctatacaa ccaagttgct
     2041 agaagagctg ggatcgaccc gttagcggtt ggctatgttg agatgcatgg aacaggcacc
     2101 caggcaggtg acagtaacga gatgaagtca gtcacaaacg tctttgctcc atctactggc
     2161 cacattcgag accgggactc acctctccac attggtaccg tcaagtccaa catgggccat
     2221 ggagaggcgg ctgctggtat catggccttt gtcaagacaa tgttggtgtt tcagaaaggt
     2281 atcatacctc ctcacattgg gatcaagacc cgattcaacc cagcactgcc agaagacttg
     2341 gctaaaagga atgtggtcat cccagtaaca gcagccatct gggttcggaa tagcgatcgg
     2401 aagcggttag ccatggtcaa caacttcgga gccgctggag ggaatacatc tatcatcatt
     2461 gaagaggcgg caccacggcc gaggactagc gaggacgtac gaaaagcgca agtcatcaca
     2521 gtgtctgcaa aaactgccaa ctctctgcaa gaaaatctca aagctctcgt tgactacatt
     2581 gaggcgagat ccgaactctc tgttgctgat ttggcttaca ctctgtcagc tcggaggaat
     2641 cactacaact atcgtgtttc tgttcttgca acttccacgg ctgaagcagc ttctttactt
     2701 cgccctcaca taaagacctc cctgtctcag acaccacact cgggcaaaca ggtccccata
     2761 gcttttgcat ttactggcca gggtacgttc tacattggca tcggcgctca attgtaccga
     2821 gactcccatg agtttcgcaa acacatcgat cagctggaca gcctcgtacg acgacaaaat
     2881 ttcgcctcct tcttgcctgt tgtgtccggg aatgttcagc cagaggatgt ttctattgtt
     2941 accatgaacc ttgccatagt ttgtgtggaa attgctctgg cgcgactttg ggagtctttt
     3001 ggcatcaagc caagcatggt cattggccac agcttgggcg aatatgctgc tttggccgtc
     3061 gctggtgtct tgtccgacag tgctgcaata tttcttgtgg gtactcgagc taggttgttg
     3121 acttctaagt gcacttctcg cacacatgga atgttgtcag tacgagcatc tgcagcagac
     3181 atcaaacatg caggagggga tattcctttc gaggtctctt gcatcaatgg tcccaacgaa
     3241 acggttcttg gaggcacgct atcaaacatg gaggctctct cggccacttt ggcagctgcg
     3301 ggctatcgaa ccttcaaatt ggatcttcct cacgcctatc acacgtatca gatggacacc
     3361 attgtggacg aactcgtcaa acaaacagag actgtagtct gtaagaaaac tacaattccc
     3421 atcatctctc ctcgtttttc aagagtcatg acatcagaag acagcatcga tgtttcatac
     3481 ttgatcggcg cgactcggga aacagtcgac tttgctggtg ctcttgatga cgcgtggcag
     3541 tctggtctgg tgaacgaatc aacgatctgg ctagagatgg gccaccatcc tacttgctct
     3601 ggattcataa gccgtacact gtcatcgact cgtatggctt tgccaagctt acagcgtgac
     3661 acggataact ggctgaccct agcaaaaaca ctttgcagcc tgtatagtgc aggcatacct
     3721 attgactgga acgaatatca tcgtcccttc gagcaggctc ttcgactgat cgatgctccc
     3781 acatacgcgt ggaccaacaa gaactactgg atccagtatc gtggagattg gaatttgaca
     3841 aaaggccgcg ctatgcctaa gcttgatcct acaactactg ttgtaccagt gtcaaagagg
     3901 tttcagacct ctagcattca ccgacttata tcagagcagt acaccgacgg aaaggcaatt
     3961 ctgtctgcag agtctagtat caccgaccct tcgctccaag gcgtagtcga tggacatgcg
     4021 atgaatggat acggagttgc ttcgtctttc cttcacgcag agatggcgtt cacgctggcg
     4081 aaacgtgtta gcgataagag cttcgcaagc gcagtttcat ttggtatcaa cgtcgctgac
     4141 tttgagtatc acgagcccgt cgtcaaactc atcaatacga gtgaaccaca gcccatcttg
     4201 gtctctgctg aggctgattt ggaaaagatg gaagtccacg tcaaatggtt caatcccgcc
     4261 aaagaaattt ggtactgtca tgcgacagtg ttctacgagg atccctcttc ctggctttcc
     4321 acatggtctc gctcaaccaa actgatcact agccgcattg acgcgcttaa cgatatggcc
     4381 gttactggta aagctagcaa actcacaacg gacctcgcct acagcctatt tggcaaactt
     4441 gttggatatt ccaagctgta tcaaacaatg cagtcggtta tcctgaatga ggatgaggcc
     4501 atggctgaag tgcagttccc cgaagatact ggcggcagtt ggaccgtccc gcctcatttc
     4561 atcgatggtc tcatctcgct gtcgggtttc atattgaacg gtggcactca tttcgataat
     4621 acgaacaact tcttcatcac cccatcttgg aagtcgatgc gttttgcgcg gccgctaact
     4681 cccggtggac gctatactgc ctacgtcaga atggtacctt ccgacaatca ctcatttgtc
     4741 ggagacgtat atgttctaca gggctcggag attgttggcg tcgtcgaagc gatactgttc
     4801 ttacagtggc caagagtaat gctcaatcgc ttcttcaggc cggctgacgt cacggcaaag
     4861 cccgcagcga aggttcctgg aaagagcgaa ccctcaactc gtccccactt caaaccacac
     4921 cacgtgtcac gccacaagcc tactctcaca ccacgcagcc cagatgaggg ctctgagaac
     4981 tctgactcct caggagtgat aatctcacgt cccgggggct attcgtcatc agaccaagat
     5041 atggaggaat tgcccagtcc tcctgctggc atgaatgacg acatggagaa agctttagca
     5101 ctggtggcag aagagctcgc cgttgacatt ggtctgctca cagatgacgc tttgatcgct
     5161 gacttgggac ttgactctct tatgtcgttg gtgatgtccc agagattgcg cgaggagcta
     5221 ggcttagaga tccgagatgc gttcttcttg gaaattacta ctatacagga tctcaaggcg
     5281 ttgcttcgat ag
//
