LOCUS       BGC0001286             16181 bp    DNA     linear   PLN 16-DEC-2015
DEFINITION  Ustilago maydis strain MB215 itaconic acid biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0001286
VERSION     BGC0001286
KEYWORDS    .
SOURCE      Ustilago maydis
  ORGANISM  Ustilago maydis
            Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
            Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 00:23:14
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /db_xref="taxon:5270"
                     /mol_type="genomic DNA"
                     /organism="Ustilago maydis"
                     /strain="MB215"
     misc_feature    1..16181
                     /note="itaconic acid biosynthesis gene cluster"
     subregion       1..16181
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001286"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..16181
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     mRNA            complement(join(<100..716,802..1085,1172..1617,
                     1750..>2025))
                     /gene="CYP3"
                     /locus_tag="UMAG_05074"
                     /product="putative cytochrome P450 monooxygenase"
     gene            complement(<100..>2025)
                     /gene="CYP3"
                     /locus_tag="UMAG_05074"
     CDS             complement(join(100..716,802..1085,1172..1617,1750..2025))
                     /codon_start=1
                     /gene="CYP3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 200.4; E-value: 8.5e-61)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="UMAG_05074"
                     /product="putative cytochrome P450 monooxygenase"
                     /protein_id="ALS30800.1"
                     /transl_table=1
                     /translation="MNTTKLLGTGALSPSFVFDHDSGNAIFGLSSSTLVVLVAMIAVST
                     LTLKSVLPGDRSINLPGPRGWPIVGSWFDLGNNWAEYFRQAAKEYGDVFKVHIGNRTVV
                     VVNSPKAAHILFNEHGSSLISRPWFYTFHGVLSKSSAFTIGTSAWSDSTKNKRKAAATA
                     LNRPAVQSYMPIIVEESLDAVRRILNDGNAGKNGIVPYSYFQRLALNTSFQVNYGFRMG
                     ERDDGLFDEISEVIAKVASVRAVTGSLQDYVPLMRYLPANAKSKAAASYGLRRKKFMSK
                     LYEELEQRVNQGKDESCITGNILKDTESRKKLSRLEIDSICLSMVSAGLDTFANTMIWT
                     IGFLAKHPEIQRKAQAELLAHYPNRELPHVDSEDLVYIHAMAKEASRLFNVFRICLPRT
                     NVSDVTYNNAVIPAGTTFFLNSWACNVDAEKFADPFEFKPERFMDKSASNAHVENKMGG
                     VETYAFGMGRRMCPGVFLALREIYTTLVFLTHFFDIAPDGEYDIDPLTAVEDGRAFSVR
                     PKPFKVRCTPRPGVDLSPVLDKQ"
     mRNA            join(<2966..3276,3369..3376,3466..3536,3611..3724,
                     3796..>3822)
                     /gene="RDO1"
                     /locus_tag="UMAG_12299"
                     /product="putative ring-cleaving dioxygenase 1"
     gene            <2966..>3822
                     /gene="RDO1"
                     /locus_tag="UMAG_12299"
     CDS             join(2966..3276,3369..3376,3466..3536,3611..3724,
                     3796..3822)
                     /codon_start=1
                     /gene="RDO1"
                     /locus_tag="UMAG_12299"
                     /product="putative ring-cleaving dioxygenase 1"
                     /protein_id="ALS30794.1"
                     /transl_table=1
                     /translation="MLRSSQARSVVRSSQWATTARVHQLELPSGWKPSALGVAPWQQRQ
                     QRQLSVRSLDHLVITCHDMDKTIDFYTRLGMSVVQFGQGRKALEFGSQKINLHQKGKEF
                     EPKALVPQPGSQDLCFVIHDSIADAQKHLQEHGIQVVEGPVKRTGAVGPILSIYVRDPD
                     NNLIELSSYQDAK"
     mRNA            complement(<4312..>5793)
                     /gene="TAD1"
                     /locus_tag="UMAG_05076"
                     /product="trans-aconitate decarboxylase 1"
     gene            complement(<4312..>5793)
                     /gene="TAD1"
                     /locus_tag="UMAG_05076"
     CDS             complement(4312..5793)
                     /codon_start=1
                     /gene="TAD1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063:argininosuccinate lyase/adenylosuccinate lyase
                     (Score: 147; E-value: 1.5e-44)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="UMAG_05076"
                     /product="trans-aconitate decarboxylase 1"
                     /protein_id="ALS30796.1"
                     /transl_table=1
                     /translation="MAPALNANPTTKRDELSAPSASHKLGMSSMASRAAGGGLKLTGLP
                     DLSDSAGTLSDIFGTPQMREIWSDQNRVACYLEIEAALAIVQADLGIIPKNAAHEIVEH
                     CRVQEIDWALYKQKTELIGYPVLGIVQQLVANCKDGLGEYCHWGATTQDITDTATVMQI
                     RQSLTLVKQRLDSIVSSLEHLAEQHRNVPMAARSNLKQAVPITFGFKMARFLATFRRHQ
                     QRLVELEKRVYTLEFGGAAGNLSSLGDQGIATHDALAKMLDLAPAEIAWHTEHDRFAEV
                     GTFLGLLTGTLAKLATDIKLMSQTEVGEVGEPFISNRGSSSTMPQKNNPISCVYIHACA
                     ANVRQGAAALLDAMQSDHERGTGPWEIIWVQLPLMMNWTSAALNNADFVLRGLQVFPDA
                     MQHNLDLSKGLIVSEAVMMGLGNTLGRQYAHDAVYECCRTAFVQDRPLLDVLLENHEIA
                     SKLDRTELEKLCDPANYLGQCSQWIDRVLSRPSSA"
     mRNA            complement(join(<6813..7084,7174..8056,8191..8267,
                     8396..>8639))
                     /gene="ITP1"
                     /locus_tag="UMAG_11777"
                     /product="itaconate transport protein 1"
     gene            complement(<6813..>8639)
                     /gene="ITP1"
                     /locus_tag="UMAG_11777"
     CDS             complement(join(6813..7084,7174..8056,8191..8267,
                     8396..8639))
                     /codon_start=1
                     /gene="ITP1"
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 94.4; E-value:
                     8.7e-29)"
                     /gene_kind="transport"
                     /locus_tag="UMAG_11777"
                     /product="itaconate transport protein 1"
                     /protein_id="ALS30797.1"
                     /transl_table=1
                     /translation="MDQADHSGVPDDAALEEAPNTVPIQEKSAQPHDTQPYCAFTKRSK
                     LFIVLTVSLAGFFSPFAINIYIPALPQIAGMLHTSEAATNVTVTVYMIAQGLSPVIWAP
                     LSDVFGRRPIYILTFFIFFIANLGLSFTNVYWLLVVLRMVQAAGACSAIAIGAGTIGDV
                     TERKERGSYMGYYALAQYTGPAIGPVVGGALSQRWDYHATFFFLTAISGPFLLFMLLFL
                     VETLRVIVGNGSAKTSGIYRPLVEPKLQRSIANAPRPGIKNPLHGTLDFGFHRPFLVFA
                     RPETSLAILAFSMVYASYYLSSGSLPYLFKQVYGLDELLIGVCFVPSGVGCAVGTVLAG
                     KILDWDYRRALDKSKLGVKVTRARLQSAWIYLPCYCASLLAYGWCVRAHTHIAAPIVFQ
                     FTLGMFSTMYFTNVNTLIVDLYPGKAASATAAVNVGRCLLGAVAVAVVQPMIDAMGAGW
                     TFTLGALLTLIVGLICQVLIYLYGEMWAARKHS"
     mRNA            join(<9923..10730,10814..>11337)
                     /gene="ADI1"
                     /locus_tag="UMAG_11778"
                     /product="aconitate-delta-isomerase 1"
     gene            <9923..>11337
                     /gene="ADI1"
                     /locus_tag="UMAG_11778"
     CDS             join(9923..10730,10814..11337)
                     /codon_start=1
                     /gene="ADI1"
                     /locus_tag="UMAG_11778"
                     /product="aconitate-delta-isomerase 1"
                     /protein_id="ALS30798.1"
                     /transl_table=1
                     /translation="MLHPIDTTIYRAGTSRGLYFLASDLPAEPSERDAALISIMGSGHP
                     LQIDGMGGGNSLTSKVAIVSASTQRSEFDVDYLFCQVGITERFVDTAPNCGNLMSGVAA
                     FAIERGLVQPHPSDTTCLVRIFNLNSRQASELVIPVYNGRVHYDDIDDMHMQRPSARVG
                     LRFLDTVGSCTGKLLPTGNASDWIDGLKVSIIDSAVPVVFIRQHDVGITGSEAPATLNA
                     NTALLDRLERVRLEAGRRMGLGDVSGSVVPKLSLIGPGTETTTFTARYFTPKACHNAHA
                     VTGAICTAGAAYIDGSVVCEILSSRASACSASQRRISIEHPSGVLEVGLVPPENAAQSL
                     VDVAVVERSVALIAHARVYYTTPDRRRSYDSPLTSPSTPADTHNLFDAAYRPVIQPSDT
                     DVEAPHMLALENKEQCVSRCDTALHHIVASYGASDAHASDRSLS"
     mRNA            join(<12169..12244,12345..12580,12681..12771,12905..>13404)
                     /gene="MTT1"
                     /locus_tag="UMAG_05079"
                     /product="mitochondrial tricarboxylate transporter"
     gene            <12169..>13404
                     /gene="MTT1"
                     /locus_tag="UMAG_05079"
     CDS             join(12169..12244,12345..12580,12681..12771,12905..13404)
                     /codon_start=1
                     /gene="MTT1"
                     /locus_tag="UMAG_05079"
                     /product="mitochondrial tricarboxylate transporter"
                     /protein_id="ALS30799.1"
                     /transl_table=1
                     /translation="MPPSGRKVSPSVSVVAGATAGAVEGVATFPIEYLKTVSQFAPRDV
                     HGNQQRLSPIEVVRSTLQKEGPKGLFRGCTAMVVGNAGKAGVRFFAFENFRSMLKNKST
                     GKLSNSSNYLAGMGAGTLEAIFAVTPSETIKTKLIDDSKRAKPRYEQGLVRGTASIVRQ
                     EGLAGIYQGVVPVVMRQGSASAIRLGTYSALRDWLPKAHGSGSSLINWLATFSIGAASG
                     VVAVYGTMPFDVLKTRMQAIDAARYRSTWHCLTNTLKTEGAAALWRGSVSRSMRLIVSG
                     GVIFSVYEQVVWLLAGPES"
     mRNA            complement(join(<13713..14525,14626..>14958))
                     /gene="RIA1"
                     /locus_tag="UMAG_05080"
                     /product="regulator of itaconic acid biosynthesis 1"
     gene            complement(<13713..>14958)
                     /gene="RIA1"
                     /locus_tag="UMAG_05080"
     CDS             complement(join(13713..14525,14626..14958))
                     /codon_start=1
                     /gene="RIA1"
                     /locus_tag="UMAG_05080"
                     /product="regulator of itaconic acid biosynthesis 1"
                     /protein_id="ALS30795.1"
                     /transl_table=1
                     /translation="MRFAGMSCDDERPANMFDLMAPQLASTSCNEHYFSTADLGASTLY
                     ATTTDAPATIAGILTPQPATLAPMYSTCPVRFDDQRSAPASTSVSGKRKHSDVEKDRRR
                     SISNGFAVLQNVLHNESNAKPISKSILLQQACDEIRELRKKLDASTTIISRFGLENLFV
                     PTPSSTHASPPNASSRIYSPINQASDVLADTRRASISTSATPILYSEEKRKANAKRRHS
                     YDGSWQASDRGSIDDEASASASASASASSSASCSSSSHTHSDDTDCDDTDTDIPAESAL
                     KERTKRHKARSKKERDRTKPRYRPKPSTNRSPTPSSCASSTPNSPPTSSNRNRDLQQAI
                     LSLLLELPSHLEDVKNKRRASQPTELADPSSVKSRSKKRHR"
ORIGIN
        1 caagacccat cataagaggc aagggcattt gaggcgtttg ggagcatcgc tcgatcgagg
       61 aaaagaggct acaaagcagc gagcggagag gcgaggcgac tattgtttgt cgagcacggg
      121 ggaaaggtcg acgccgggtc gaggcgtgca gcgcaccttg aacggtttcg gacgtacgct
      181 gaacgcgcga ccgtcctcta cggctgtgag agggtcgatg tcatactctc cgtcgggagc
      241 aatatcgaaa aagtgggtga ggaagacgag cgtcgtgtag atttcgcgaa gcgccaagaa
      301 cacgccggga cacatgcgtc ttcccatgcc gaaagcgtac gtctcgacgc cgcccatctt
      361 gttctcgacg tgtgcattac tggcgctctt gtccatgaag cgctccggct tgaactcgaa
      421 cgggtcggcg aacttttcag cgtccacgtt gcacgcccat gagttgagga aaaacgtcgt
      481 ccccgctgga atcacggcgt tgttgtaggt gacgtcgctc acgtttgtac gcggcagaca
      541 gatgcggaac acgttgaaca gtcgtgatgc ctccttggcc atggcatgga tatacaccaa
      601 gtcctccgag tcaacatgcg gaagctcccg gttcggatag tgagccaaca gttcggcctg
      661 tgccttgcgc tggatctctg gatgcttggc caggaaaccg atcgtccaga tcattgctgc
      721 caacacgcca atacacagaa aagaactggt cagacacggg atcggtgcac ttgtgttgta
      781 tctctgagtt gagcaactca ctgttggcaa aagtatccag acctgccgag accatcgaga
      841 gacagatcga gtcgatctcc aagcgagaca gcttcttgcg cgattcagtg tctttaagga
      901 tgttgccggt gatgcaactc tcatccttgc cttggttgac gcgttgctcg agttcttcgt
      961 acagtttcga catgaacttt ttacgacgca gaccgtacga agcagccgcc ttgctctttg
     1021 cattagccgg caggtagcgc atgagcggaa cgtagtcctg caacgaaccg gtcacagcgc
     1081 gaacactgta cgagaagcag agcgccaagt tagcaaatcg cgaatgcaag tcaagcaggt
     1141 aggtgcaaga gtcgagtcga ctgatacgta cgaggccact ttagcaatca cctcggagat
     1201 ttcatcaaac agaccgtcgt cgcgctcgcc catgcggaag ccgtagttga cctgaaagct
     1261 cgtgttgagc gcaagacgct gaaagtagct gtacggaaca atgccgttct tgcccgcgtt
     1321 gccgtcgttg aggatccgac gaacggcgtc caacgactct tcgacaatga tgggcatgta
     1381 cgactggacc gccggacgat tgagcgcagt ggcagctgcc ttgcgcttgt tctttgtgct
     1441 atcgctccag gccgacgtac cgatagtgaa ggcagacgac ttggacagca cgccgtggaa
     1501 cgtatagaac catggtcgtg agatgagcga cgagccgtgt tcgttgaaca gaatgtgcgc
     1561 cgccttgggt gagttgacga ccaccacggt gcgattcccg atgtgtactt tgaatacctg
     1621 gcgtacatac agatgcaaga tacacgtcag aacgtcagaa cgtcagcacg ccagcacgtc
     1681 agcacgtcag ctcgacccat gccaaccaca cgagacatgg atgcactgcg gaaaccacga
     1741 gagacgcaca tcaccgtatt ctttggcagc ttgacggaag tactcggccc agttgttgcc
     1801 gagatcgaac caagagccga cgatcggcca gcctcgaggg cccggaagat tgatgctgcg
     1861 atctccgggc agtacgctct tgagcgtgag cgtcgagacc gcgatcatgg ctacaagaac
     1921 aaccagcgtc gagctcgaaa gtccaaagat ggcgttgcca gaatcgtggt caaacacaaa
     1981 tgacgggctg agcgccccgg taccgagtag tttggtcgtg ttcattgcga catacaacac
     2041 gtgagatgtg aatagcgatg gacccgacac cgatgcagcc gcaagaggat gcaagacggg
     2101 tatatataca tgctgaccaa tccgcgtgcg taacgcgttt cacgttttcg tggtggcatc
     2161 aagtgatcca tgccatgaac agtccgcagc gggatctctc gaggtgacag gtgcagtccg
     2221 gatcggatct gttgggctgt ctgctcgcac gcacgggatt gtacggcttt gtagggggca
     2281 cgagacggcg caggagcgca ccggcgcgca ggggcacagg ggcacagggg cgcacggtga
     2341 tcacgaagcg ccgtctgaca ggtgtgtagg aaagagcgca aagtgtgacg tccaagtggc
     2401 aagagcgcgt ggacatgtag ctcaaacgtc gtaagtacgg gagcgatgtt aagcgtcagc
     2461 aatcgaatca cgaatggatg cgctcgtcgg tgttgcaaaa gcgtgatcag cgcgtgactt
     2521 gcacgagcac agcgtcgacg cggttgcaca ttcatcttgg tgatggacct cgctctcgac
     2581 tcttatccag ccctgagtga tgcgtcatgc gtcatccgcc attcgtccat tcacaagata
     2641 ctgtactcga tcgtggatac actctgagac acggctgctt ctaagcatgc taacacgccc
     2701 aaatcgctcg tgagtcgtga gtgagcaagc cgcttgcgaa attgggcaac cgctcaccgt
     2761 tatggctaag cgagcgacca aacacccaag caagcaatcg cgaatgccgt gcgtgatcca
     2821 tggatcactc acacactttc gtgtttggct cgacgcgtcg agcttgatca atcgttacca
     2881 tcttactacc gacgtttacg tttggtttca cgtttggtga gcttaagctt gtccgactca
     2941 cgacttgatt cacagaccac cacacatgtt gcgttctagc caggccagaa gcgtggtacg
     3001 ctccagtcag tgggccacca ccgcgcgagt tcaccaactg gagctgccaa gcgggtggaa
     3061 gccgagcgca ctcggagtag caccatggca gcagcgccag cagcgccagc tgtcggtcag
     3121 gtcgctcgat cacttggtga tcacctgcca cgatatggac aagaccattg acttttacac
     3181 gcgcctcggc atgagcgtgg tgcagttcgg acaagggcgc aaagcgctcg agtttggctc
     3241 ccagaagatc aacctgcacc agaaaggcaa agagttgtaa gtacagtgtg gtgtgtggtc
     3301 agcaagcagc tcaacaagga cccaggccac tgacaccgac tttgtttgtc tgcttggtct
     3361 cggctcagcg aaccgagtaa gttgcaaggc ggcgcatgtg gtgcggcgaa tgtaggtggt
     3421 tgcgtggctg cgaatgcatg tactgacggt tggctgtgtc ttcagaggcg ttggtcccgc
     3481 aaccgggatc ccaggacttg tgtttcgtca ttcacgacag catcgccgac gcccaggtaa
     3541 gaagcgtgta ctagctgcac ccgtgcatcc gtgcaaggcg cccttaagct gacgcctgag
     3601 ctctcgacag aaacacctgc aagagcacgg catccaagtt gtcgagggcc ccgtcaagcg
     3661 caccggtgct gtcggaccga tcctcagcat ctacgtgcgc gatcccgaca ataacctgat
     3721 cgaggtgagt gccaagcccc gcgattttca gtgcaataca aacaagcctc gctaacacac
     3781 acgtctgctc tccagctctc gtcgtaccaa gacgcaaagt gaacgctcca acaaatgtcc
     3841 tgagttcagc tggttcggga atgcacaaga tccaacaaac gtctgctcgt gttcggaaga
     3901 gatgtgtggc agttgaaata tgagaaagaa gcgatgagat tcctgtcaac cagtgtgatg
     3961 agtacatgag tatatatacg ctggttgctc agggggctac aacgggcaca ttcgtgagtg
     4021 gtatctcatg tgatctggag gcgcgaaaca cggcgcccaa cggcgcccaa cggcgtgttc
     4081 acgaagcgtg caccaacatc cgatcgcggt tgcggactgc tgcgtctttg tgcttgcatc
     4141 ttcaagtgtg gcgtatgtct gacacacttg gagcgctttg atcgtttgcg tggagggaat
     4201 ggaattcagc gattcaagca cacgcgccaa cagaagagtg cggatgagat ggcaagattc
     4261 gtgattggac aggagcagac gtcaacgagg gaggccaaca aggtacacgt atcaggccga
     4321 cgatgggcga gacagcacgc gatcgatcca ctgcgaacac tggccgaggt agttggcggg
     4381 atcacagagt ttttcaagct cggtgcggtc tagtttggag gcgatctcgt gattttcgag
     4441 gaggacgtcg aggagcggtc tgtcttggac gaacgcggtt cgacagcatt cgtagacggc
     4501 gtcgtgtgcg tactgacggc cgagcgtgtt accgagaccc atcatgacgg cttccgagac
     4561 gatgagccct ttcgagaggt ccaggttgtg ttgcattgcg tctgggaaca cctggaggcc
     4621 gcgcaggaca aagtcggcgt tgttgagagc ggccgaggtc cagttcatca tgagtggcag
     4681 ctggacccag atgatctccc agggacccgt gccacgttcg tgatcagact gcatggcatc
     4741 gagtagcgct gcagcgccct gacgcacatt cgccgcacac gcgtgaatgt agacacacga
     4801 gatcggattg ttcttctgtg gcatcgtcga cgacgagccg cggttcgaga taaacggctc
     4861 gcccacctcg cccacctcgg tctgcgacat gagcttgatg tcggtggcga gtttggcaag
     4921 cgttccagtg agcaggccga ggaaggtacc tacctcggcg aagcggtcgt gttccgtgtg
     4981 ccacgcaatc tcggcgggcg ccaggtcgag catcttggca agcgcatcgt gcgtcgcaat
     5041 gccctggtca cccagcgacg acaagttgcc tgcggcaccg ccaaactcga gcgtgtagac
     5101 gcgcttttcg agctcgacga gacgctgctg gtgtcggcgg aacgtggcga ggaagcgcgc
     5161 catcttgaag ccaaacgtga tcggtaccgc ctgcttgagg ttggaacgag ccgccatggg
     5221 cacgttgcga tgctgctcgg cgagatgctc gaggctcgag acgatgctgt cgagtctctg
     5281 cttgacgagc gtgagcgact ggcggatctg catgacggtg gcggtgtcag tgatatcctg
     5341 cgttgtggcg ccccagtgac agtactcgcc gagaccatct ttgcagttgg cgacgagctg
     5401 ctgcacgatg cccagcacgg ggtagccgat cagctcggtc ttttgcttgt acagagccca
     5461 gtcgatctct tgaacgcggc agtgttcgac gatctcgtgt gccgcattct tggggatgat
     5521 cccgagatcc gcctgcacaa tcgcgagcgc cgcttcgatc tcgagatagc acgccacacg
     5581 gttctggtcc gaccagatct cgcgcatctg cggcgtaccg aaaatgtcgc tcagcgttcc
     5641 tgccgagtcc gagagatcgg gcagaccagt gagcttgaga ccgccgcctg ccgccctgct
     5701 cgccatgctc gacatgccga gcttgtgcga tgccgacgga gcgctcagct cgtcgcgttt
     5761 cgtggtaggg tttgcgttga gtgcaggtgc catactggat cgacgtaaaa gccagctggt
     5821 tggacgccag agaagacaag atgtgcgatg gacgtgcagc atggtggttg agcaagctcg
     5881 agtgcacttt atatgtgtcg tatccgtcaa agtgatgctg tcgataacca aaccaacacg
     5941 aaagagcgtg caagccaact ttggtttggg cgcaggttga ggtgatgatc aaggtcatgc
     6001 gtcgcgagcg tgacgaaccg agccaagccg agccgagcgc tgcggagcgc agcggagcgt
     6061 ggagagtcac agagtgtaaa gtctagaatg gccaaaactc acgctcacgc acaccaacga
     6121 cgactcacga ctggaccctg catcacgctt cgtgcttcgc gcttcgtaca tcgtgctgga
     6181 tgctggttgg tcgtcgtcgg ctaagataac gcgatcgtga ctgaaaaggc gcacgaaaag
     6241 ctcagtcacg aattggtggt tgtgaatctg taaattacga atgccgcagc gcgtgcaggt
     6301 cacgcttcca actcgtgagt ggtgcgctaa gcatgcatca tgccccccca cgtccatcac
     6361 gcctgttgca ccttgtctat gcaagctgcg tcgattgtgt ggtgcttgcg agctaagtta
     6421 gtcgctgttt ctagctgatg cacgccaatc gcgaatcgtg atggtgaatg tcaacttgga
     6481 tctgaccaac tcaggactcg gacgtcagcc atgcttgcgc ttcgctctct tgtctgttgg
     6541 tgccgtgcgg aaagcgagat ggacagtctc tggcagacgc tcgctcattg cacgtcccca
     6601 aagttccagg cagtccatac gcaggagagt ctctaagtcg tgcagccgac acagccaatt
     6661 caagaaaccc agaagcaggc gagctcaagc taggcttgcg ttggggagag tccaagtagg
     6721 aaatgtattg gcatcacgta tgtaatgtac acaagtcacc agtcgtgagt gttgccaggc
     6781 gtcaaagcgt ccaggcgtca aggcgtgagt gatcacgagt gtttgcgagc tgcccacatt
     6841 tcgccgtaga ggtagatgag aacttggcag atgagaccga cgatcagtgt gagtagtgcg
     6901 ccgagcgtaa aagtccagcc ggcgcccatt gcgtcgatca tgggctggac cacggcgact
     6961 gctactgcgc cgagcaagca ccgtccgacg ttgaccgctg cggttgcgct ggccgctttg
     7021 ccaggataca ggtcgacaat gagcgtattg acattggtaa agtacatggt cgaaaacatg
     7081 cccactgtag aaatcaccaa atgcgaacag cacacaacca tggtcagcaa tcgcagtaca
     7141 gtatctagcc gagcgagtgg gtctgcaacc tacgtgtaaa ctgaaacacg atcggagcgg
     7201 cgatatgcgt atgcgcacga acacaccatc cgtacgccag aagcgaagcg cagtagcacg
     7261 gcaggtagat ccacgccgac tgcaaccttg cgcgcgtcac cttgacacca agcttgctct
     7321 tgtccaacgc acgacgatag tcccagtcga ggatcttgcc ggcgagcact gtgcccaccg
     7381 cacaccccac accgctcgga acaaagcata cgccgatcag gagctcgtcc agaccgtaca
     7441 cctgcttgaa cagatacggc agcgaaccag atgacaagta gtagctcgca tataccatcg
     7501 aaaaagccag gatggctagg ctggtctcgg ggcgcgcaaa caccaaaaac ggacggtgga
     7561 agccgaaatc gagcgtgcca tgcagtgggt tcttgatgcc aggccgaggc gcattggcga
     7621 tcgagcgttg cagcttgggc tccaccaagg ggcgatagat gcccgacgtt tttgcactgc
     7681 cgttgccaac aatgactcga agcgtttcaa cgagaaagag aagcatgaac aacaaaaacg
     7741 gacccgagat cgccgtcaag aagaaaaacg tagcatggta gtcccatctc tgcgaaagcg
     7801 cgccaccgac aaccggtccg atggcggggc ccgtgtattg cgcaagcgca tagtagccca
     7861 tgtaacttcc tcgttccttg cgctcagtaa cgtcgccaat cgtaccagcg ccaatcgcaa
     7921 tcgcactgca cgcgcctgca gcctgcacca tacgcagcac caccaagagc cagtagacgt
     7981 tggtgaacga cagcccgagg ttggcaatga aaaagatgaa aaacgtcaag atgtagatcg
     8041 gtcggcgccc aaacacctgt gatgcatcaa caagatcgag acaagatcaa gcccaaaaaa
     8101 cgcaagaaac gcttgcatat gtggtcagag agagacacgc caagccaaag ccaaagctaa
     8161 agccaaccga gcgcaatttt ggctactcac atccgagaga ggagcccaga tgaccggtga
     8221 aagtccctgt gcaatcatgt agacggtcac cgtgacgttg gtagcggctg gcaaggtgta
     8281 aggggggaaa gagaagaagc ggaaaggagg acagaggtga atgagaaaac aagttgacgg
     8341 ctcccgaatc ggttgctctg cacgtttcgc gcttcagccg ataggtttca ctcaccttca
     8401 gaggtatgca gcataccggc gatctggggc agggcaggga tgtaaatgtt gatggcaaac
     8461 ggcgaaaaaa agccggcaag agagacggtg aggacgataa acagcttgga ccgcttcgtg
     8521 aaagcacagt acggctgcgt gtcgtgaggc tgtgcgctct tttcttgaat cggtactgta
     8581 ttgggcgcct cctcgagcgc agcgtcgtct gggacgccgg aatgatcggc ttggtccatt
     8641 tcgacttggt ggatactgcg gctgttggcg tcgggtgttg atgctgcgtg agagcatgtc
     8701 aacatgtcag cggagccact catagccaag acgtatgtca aacgtttgtc gattcgagtg
     8761 cagatgtgtg tggttgatga tgatgatgat gatggtggtg gtagcatatc aacgtcgatg
     8821 cttacgtcga gcttgagctt aaagccagca aagctacgct acggcaaata acttggcaag
     8881 cttcggcgac tagaggcacg agcgctgatt tcaccgttgt tgggtcgtga attggcggca
     8941 tcgagtgcat tcacaagagg accactgtga acgagatacg caggccagtc acgattgatt
     9001 cagcatgata gacaagtgcg aagcgcaaag cacaaagcac aaagcacaaa gccgactgag
     9061 ctagatagct aaccaaacga gtgcgataac tgtcaattca ccttcgtgat tcacgattgt
     9121 acgacgcttc cttgtctgcg aaaatacgca atccacactc gtcacatacg ttacactgga
     9181 ctttttacta cggtatcact tgtgcgctat ttcgcaaatc cgaagtgtcc gaagaaatgc
     9241 cattcgtgat tatcggctgt gcgtgagcaa cagtcccaaa gccacagagc gatgcactag
     9301 cagcacctag tagaagcaag tcgtgagtca gtgcgagtgc gagtggacta gcgtacacag
     9361 cacgaatcgc tgaatcacga atccgatcca tccttctatc cttgtatcct cggaggaaat
     9421 cttaataaaa gaaaagaaaa caattgtgga taacttgatc aaaacacgaa aaattgcgct
     9481 attttgattt agattaagcg cctttttgga agcgtttatg ccgcccacgc cgatctgcga
     9541 ctcaggactg tggccaactt gacgcgaata accatgaatc gtgaatgtga ataatcgtga
     9601 ataagtaaca aacgtgaaaa tggggaatat atttcaaaac ccgaacctgt cgtgcgtcag
     9661 cgtgaccggc gcgtgctccg accagtcaag tcgagtgcgt cgagtgcgct gttggacagc
     9721 ctctcacgac tcgcacgctc cgccaaccat actcgagcgc agcacgcgaa aagcacgagt
     9781 cgtcacacgt tggcttgcca gtcacttgaa ccagccagag tcaagacagg agacagtcac
     9841 gagtcgatgg taaatataac ctcaccgtcc aagtgatgga cgcttgtcga cacatgtcgg
     9901 tcttgactcg acccagctca ccatgttgca tccgatcgat accaccatct atcgtgccgg
     9961 caccagccgc gggctctact ttctcgccag cgatctgccc gctgaaccaa gcgagcgaga
    10021 tgcggcgctc atctcgatca tgggctctgg tcatccattg cagatcgatg gcatgggcgg
    10081 tggcaattcg ttgacctcca aggtggccat cgtctctgct agcacacagc gcagcgagtt
    10141 tgacgtcgac tatctcttct gtcaggtcgg catcactgag cgctttgtcg ataccgcccc
    10201 caactgcggc aatctcatgt cgggcgtcgc tgcatttgcc attgaacgag gtctggtgca
    10261 accgcatccg tcggacacga cttgtctggt tcgcatcttt aacctcaact ccagacaggc
    10321 ttccgagctc gtcatcccgg tctacaacgg tcgcgttcac tacgatgaca ttgatgatat
    10381 gcacatgcag cgtccttcgg cgcgcgtcgg gctgcgcttt ctcgacacgg tgggctcgtg
    10441 cactgggaag cttctgccca ccggaaatgc gagcgactgg atcgacggcc tcaaagtgtc
    10501 catcatcgac tcggcagtcc ccgtggtgtt cattcgtcag cacgatgttg gtatcaccgg
    10561 tagcgaggcg cccgccacgc tcaacgccaa cactgctctc cttgatcggc tcgagcgcgt
    10621 tcggctcgag gcgggccgac gcatgggtct cggcgacgtc tctggtagcg tagtccccaa
    10681 gctttcgctc atcggtcccg gtacagagac gaccacgttt accgcacgct gtaagtcgag
    10741 tatttgttcg agatcgcata gcgttgatac ctagtggctg acgaatacga ttcgtgttgg
    10801 agatggtcga cagattttac gccaaaggct tgtcacaacg cacatgcggt gacgggtgcc
    10861 atctgtacgg ccggggcggc gtatatcgac ggaagcgtgg tgtgcgagat tctttcgtcg
    10921 cgtgcttcgg cgtgtagcgc gtctcagcgt cgcatttcga tcgagcatcc gagcggcgtg
    10981 ctcgaggtgg gtctcgtacc gcctgaaaat gcggcgcagt cgctcgtgga tgtggcagtg
    11041 gtagagcggt ccgtcgcgct gatcgcgcat gctcgcgtct actacaccac cccagatagg
    11101 cggcgctcgt acgactcacc gctcacttcg ccctccacgc ccgccgacac gcacaacctg
    11161 ttcgatgcag cgtaccgtcc cgtgatacag cctagtgaca ctgacgtaga ggctccacac
    11221 atgcttgcgc tcgaaaacaa ggagcaatgc gtgtctcggt gcgataccgc gctccaccac
    11281 atcgtagcca gctacggcgc tagcgatgca cacgcatccg accgcagcct ctcttagttg
    11341 ctgtaaatag aaacccacac ccacagttac gcatccctgt tttgcgtctt ggatgcccaa
    11401 gctgttggca gagtgtgaaa atgtaaaggt gttcatgaaa tcacgcctgt cggtcttcag
    11461 acatgctcgt tgttcgttgt gcgttgtgcg tctgtatgtt tgtgcgccaa gcgtgacttg
    11521 ggagagctat gcagccagcg ctgcgttacg ctgtgttgcg caagagcatg tttggcaagt
    11581 cacgcggtgt gcacgaggca tgctcgtgct gagcgtaaaa tccaaaaacc aaaatcgcac
    11641 tgcacgaggc ggtgtggtga cgctgacgac tgcggtcacg ctgaaactcc cccccaccgt
    11701 gaaagtatga tgcgatccac gtcgttcaga catcacaaac gtgcatggct gcatgtcacc
    11761 acgccacata tcatgcacgg aagctggtag aaagtctatg cgcgagttag cgtgaaagca
    11821 atcccatcga tagcggtggc gagcgtgact tggaagcctg acaaatggat cgactcgtag
    11881 taggtgttgg cagtaatttc agcggatgct cggatcagca ctggccgcac ccccgcccag
    11941 ccaacgctgc tcgacggtcg agataagatt tccatgtcct agattgttgg tttggctgcg
    12001 atccagctgg cagaacgtgg tggatgtgcg cgcatcttgg gattcgtctt ggttctgctg
    12061 agctggtgga taccataaaa ctcgcgcttg tcgaatcaca gatgcgtttg tcctcaccag
    12121 ctctgcctcc aagttttgca tacacgctct tgagattcgt catccaccat gccgccgtct
    12181 ggccgtaaag tgtcgcctag cgtctcagta gtagcaggag ccacagccgg cgctgttgaa
    12241 ggaggtgagt ttattggcgc caacacgaaa tgacgaatca ccacgatctg accgagagcc
    12301 ggaaccgaat gcttgtgttg tcgcttggct tgttggtgga tcagtcgcta cgtttccgat
    12361 cgagtatctc aagacagttt cgcagtttgc accgcgcgat gtgcacggga accagcagcg
    12421 gctatctccg atcgaggtgg tccgatcgac gctccagaag gaaggtccca aggggctctt
    12481 tcgaggctgt accgctatgg ttgtaggcaa tgccggtaag gctggtgtac gatttttcgc
    12541 ttttgaaaac ttccgcagta tgctcaagaa caagtctaca gtaagtcggc gcatctttct
    12601 gctctacagt gcgagctaga gcgtgttttg ccattccacg acgctgacgt ttgccccgtt
    12661 ttttggcggt tgacctttag ggaaaattgt ccaactcgag caactacctt gccggtatgg
    12721 gcgccgggac gttagaggcc atctttgccg tcactccgag cgagacgatc agtgagtggc
    12781 ttcagcggta gatcaaacca cgcaattgcg cgcacccgaa cgccaactga ccggctggtg
    12841 cggatgtttg tcttgttttg tttgatcatt tttcttctta tggctattgt gcggtacgca
    12901 acagagacca agctgatcga tgatagcaag cgagccaagc cacgctacga gcaagggctt
    12961 gtgcgtggta cggcgtcgat tgtacgacag gaagggttgg caggcatcta ccagggggtt
    13021 gtaccggtag tgatgcggca ggggtctgca tctgcgattc ggctggggac gtactctgcg
    13081 ctgcgagatt ggcttccgaa agcgcacggt agtggatcgt cattgatcaa ctggctggct
    13141 acgttttcga tcggcgcggc atctggcgta gtcgcggtgt atggaacgat gccattcgac
    13201 gtgctgaaaa cgcgcatgca ggccatagac gctgcacgct accgctccac ctggcactgt
    13261 ctcaccaaca ccctgaaaac agaaggcgca gctgccctgt ggcgtggctc ggtatcacgc
    13321 agtatgcgtc tcatcgtcag cggaggcgtc atcttctcgg tctacgaaca ggtcgtctgg
    13381 ctcctagcag gtcccgagtc gtagttagct gtgccgcctc gcgtgtttcc ttccgccttg
    13441 ctgtatatat ctttgtcgca tctttgggtc caacctcgca accagcctgg aactgcattc
    13501 acgatcgcgc aatctagact ctgtgctctc ttcttgcttg gctcttggct cttggctctt
    13561 gactctcctg agctgcaatc gtcactcgtc gctgtgaatg cctgcatttc tatggtgtgc
    13621 aacaaaacaa aaacaaaaac aaaaacaaac caaagcgcgt ggcggactaa ctaagaatgg
    13681 tgaattgaga atcacggatt cgacttgaca cgttatcggt gacgtttctt ggacctggac
    13741 tttacgctgg aaggatcggc gagttcggtt ggttgcgaag cacgtctttt gtttttgacg
    13801 tcttcgagat gcgaggggag ttcgagaagg agagagagga tggcctgttg gaggtcgcgg
    13861 ttgcggttgg atgatgtggg cggcgagttg ggcgtactgg aagcgcagga ggacggagtt
    13921 ggtgaacggt tggttgatgg tttcggtctg taacgcggtt tggtgcgatc tcgttctttc
    13981 ttggatctgg ccttgtggcg tttggttctc tccttgagtg cggattcggc tgggatgtcg
    14041 gtgtcggtgt cgtcgcagtc ggtatcgtcg gaatgcgtgt ggctactact gctacagcta
    14101 gcgctgctac tggcactggc actggcactg gcactggcac tggcttcgtc gtcgatcgag
    14161 ccacgatccg atgcttgcca cgacccatcg tacgagtgtc tgcgtttcgc attcgccttg
    14221 cgcttctctt cgctatacag gatcggcgta gcactcgtcg atatggaagc acgacgtgta
    14281 tcagccagta catcggaagc ctggttgatg ggcgagtaga ttcgactaga cgcattcggc
    14341 ggcgacgcat gcgtggatga gggtgtgggt acgaacaggt tttccagacc aaaccgcgag
    14401 atgatggtag tactagcgtc gagtttcttg cgaagttcgc ggatctcgtc gcacgcctgc
    14461 tggagcagaa tcgacttgga gatcggttta gcgttggact cgttgtgaag cacattctgc
    14521 agcacctgat caagtcgacg cacaggttca tgcaccattc aacgcattcg gtcagcttga
    14581 gcttcggctg gcgcgtgatc caacgaggcc aagtagccta cttaccgcaa acccatttga
    14641 aatgcttctt cgacggtcct tttccacgtc cgagtgtttg cgtttgcccg acacggatgt
    14701 ggatgccggt gcagagcgct ggtcgtcaaa acgcaccgga cacgtgctgt acatcggagc
    14761 caacgtcgct ggctgtggcg taagtatgcc agctatcgtg gctggtgcat ccgtcgtcgt
    14821 ggcgtacaac gtcgaagctc ccaagtccgc agtggaaaag tagtgctcgt tgcacgatgt
    14881 cgaggccaac tgtggcgcca tcagatcaaa catgttggca ggacgctcgt cgtcgcaact
    14941 catccctgca aagcgcatct ggtcgctgca agagtgggcg aatccgaacc aaaccaagcc
    15001 aaaccaaacc aaagcgcaca gagccgaacc gaaccgcaaa ccaagcgttc caaaatgggc
    15061 aagtatagat cccagagagg ggaggagcgg agagcagaga gcggagagtg gaaagcagag
    15121 agcggagagc gcaaggcgca gagacaagac agattaagta ggattgatca tcttggacga
    15181 gtctgcgcct ggctgagcgg cgtgacgagc accactgaca gactcatggc gagtgcatgt
    15241 ggtgcacact gcgctttttg acatggatag cgccggcaga gccagagcca gagccagaga
    15301 gcttcatgat acggatcgcg gacatcacaa ttcacgatcc acgattcatg attcatgatt
    15361 cgtgattcgt gattcgtgat tgacgaatgg aatgattaaa tctccgcctt gttagtgcgt
    15421 atagcgtagc ggcaagctag cgagctgcgc agtgaagcgg gtggcgcaaa gaaacacgcc
    15481 caccgacggc tcgaacagcc acagctccag tggcaaccag ccagaacatg ggcgtatgcg
    15541 aagtgctggc cgccgattcg gtgattcatg attcgcaagc tcgaaccgag cgacaacaag
    15601 aacgagagcg agagccagaa cgagagcgag aacgagaacg agaatcagaa cgaggccgaa
    15661 tgtaaattgc gtatagatgc aatcacgaat gagcggtttc tgaacaatgt caagccaggt
    15721 gtgagtgtcg cgctgcaccg ggtaaacgca tctcggatgg attcacggat gcctaaagta
    15781 agagtgtatg catgcatgtg catgcatact gtgcatgtgc agtgtcagtc agtgctttcg
    15841 gtctttggca gagacgtgat ggatgcacgt cgcagccatc agcgtgggcg cacaagcaaa
    15901 accgtgcgtg acgcagtgct gcgtgagtat ctccagcaga cccgccaaca caagcgagac
    15961 gagctaggtg ggcatattcg tgattcacac ttgtgagtca cgattcaccc aaccccagtg
    16021 cgagtccgca gtccgcaatc cgcagtccgc agtccgcagt ccgcagtcaa caatactcac
    16081 ggatcgcgaa tccatccatc ccgctctgac tctcgagacg ttgccaagtt tcgccccacc
    16141 gagcgacact atccatgtgc aactgcaacc aagcgcagca c
//
