LOCUS       BGC0001289             10643 bp    DNA     linear   BCT 04-JAN-2016
DEFINITION  Paenibacillus ehimensis strain A3 lantibiotic gene cluster, complete
            sequence.
ACCESSION   BGC0001289
VERSION     BGC0001289
KEYWORDS    .
SOURCE      Paenibacillus ehimensis
  ORGANISM  Paenibacillus ehimensis
            Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 00:27:44
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10643
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..10643
                     /country="India"
                     /db_xref="taxon:79264"
                     /isolation_source="Rhizopsheric soil"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus ehimensis"
                     /strain="A3"
     protocluster    1..10643
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[670:7066]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      671..7066
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..10643
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..10643
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001289"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10643
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            17..211
                     /gene="penA1"
     CDS             17..211
                     /codon_start=1
                     /gene="penA1"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="putative lantibiotic peptide"
                     /protein_id="ALR96377.1"
                     /transl_table=11
                     /translation="MANNNFDLDVQVKSVNSVNANFGLYTSQCYSSQCYSSKCYSDSCY
                     SSDCYTGRHMCGYTHGYSC"
     CDS_motif       17..79
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MANNNFDLDVQVKSVNSVNAN"
                     /locus_tag="penA1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       80..211
                     /prepeptide="core"
                     /locus_tag="penA1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="3.10"
                     /molecular_weight="4650.0"
                     /monoisotopic_mass="4646.8"
                     /core_sequence="FGLYTSQCYSSQCYSSKCYSDSCYSSDCYTGRHMCGYTHGYSC
                     "
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MANNNFDLDVQVKSVNSVNAN"
                     /leader_location="[16:79](+)"
                     /alternative_weights="4668.0"
                     /alternative_weights="4686.1"
                     /alternative_weights="4704.1"
                     /alternative_weights="4722.1"
                     /alternative_weights="4740.1"
                     /alternative_weights="4758.1"
     misc_feature    17..10643
                     /note="lantibiotic gene cluster"
     gene            293..484
                     /gene="penA2"
     CDS             293..484
                     /codon_start=1
                     /gene="penA2"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="putative lantibiotic peptide"
                     /protein_id="ALR96369.1"
                     /transl_table=11
                     /translation="MANNVFDLDVEVKSVNSVNQNIGLFTSTCFSSQCFSSKCFTDTCF
                     SSNCFTGRHQCGYTHGSC"
     CDS_motif       293..373
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MANNVFDLDVEVKSVNSVNQNIGLFTS"
                     /locus_tag="penA2_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       374..484
                     /prepeptide="core"
                     /locus_tag="penA2_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="1.80"
                     /molecular_weight="3753.1"
                     /monoisotopic_mass="3750.5"
                     /core_sequence="TCFSSQCFSSKCFTDTCFSSNCFTGRHQCGYTHGSC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MANNVFDLDVEVKSVNSVNQNIGLFTS"
                     /leader_location="[292:373](+)"
                     /alternative_weights="3771.1"
                     /alternative_weights="3789.2"
                     /alternative_weights="3807.2"
                     /alternative_weights="3825.2"
                     /alternative_weights="3843.2"
     gene            671..3793
                     /gene="penB"
     CDS             671..3793
                     /codon_start=1
                     /gene="penB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     954.9; E-value: 7.2e-289)"
                     /gene_kind="biosynthetic"
                     /product="lanthionine biosynthesis protein"
                     /protein_id="ALR96370.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.2e-186,
                     bitscore: 610.8, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.8e-40, bitscore:
                     128.6, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 9.7e-18,
                     bitscore: 54.1, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVELFQNLSVDPNEDEAVSRKPWFEKLAAYAELPIIREAIAVSS
                     LPLLQALPNLTNQEHPRKQDQAVKGFLRYLLRMTSRPTPFGLFSGVTYGNFGERYAFGL
                     QPVTAHTKRARPDMEWLLKVLSDLESRFDVVKQLKVSLNCLAYPVGSRLYLPYLTKYGV
                     KQKSKSTMESTSVRLTPAVKQVMELTRQPLPFCELALKLHSQFSDTSLEQINQFLWQLV
                     QQEIIITDLRPPLMTASPFDYVLQRLADVTGVEDERTKLETAAALIASYNRTEIGQGEP
                     LYHELVEHMKSVAEADNLLQVDLALSMETLTLPKSVAQEIERVGEALWRISGERLRLSH
                     LESYRADFMEKYGAYREVPLLELLDADIGLGAPAGYEYPRGNRKEDNTPSFAQQKKKML
                     LTQWVMTCLQQGRQELELTDNMVMQLEPVPMEEKYAPRSLELVFGLAARSGQAVEEGDF
                     LLIAGGTTGTYGAGKSVGRFVDLFDSSFVDKLKQIDRLEQKLQPEAILAELVYFPGDGR
                     AANVALSTNIREYEIDIGTNSAKDPEHTIPLSDLVVGISASGFYLRSLAKNRRVIPVTN
                     HMLNTSNSPNVYRFLREISMENQRNIDFFEWNELRTLPFLPRVRYSKTIIAAACWKFNG
                     DLPPFRGNMTETQWRQAFEAYRKQWNVPRFVYLTQSDNRLLLDLDSPLHRDEVYRDYTK
                     LQPGGQIVLTETGFAFEHLPESADGHYVTEFVFPLVKRDLPQASTQPDLGGTLASPSIR
                     LAERLKMPGSEWLYLKWYGADHRADAFIGGPLLEFCRQAEEQGWAAGSFFMRYADPDPH
                     LRWRFCGHPEQLHTVLLPQLYRFTRQCVEDGLLSRLVIDTYDPEIERYGGRTLIGTAER
                     LFCVDSRVVAEWIHARQSGHLSLDPHLLAVISVIDMMERFGLSFFEQFDWLNTMVQHKE
                     HLELFRQHRPFLMKFGDARGNFAQLRHLPDGAVILPMLQARGEAIARYVEGIRLCQAEG
                     TLTNTQEDLMQSVIHMHLNRLFGIQREEEQKVLTLVRHTLYSLQHVRRNQP"
     gene            3790..5667
                     /gene="penT"
     CDS             3790..5667
                     /codon_start=1
                     /gene="penT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 208.3; E-value:
                     2.1e-63)"
                     /gene_kind="transport"
                     /product="lantibiotic ABC transporter"
                     /protein_id="ALR96371.1"
                     /transl_table=11
                     /translation="MSSAAEEASLTVNLTNIVQSFRYWPRVFQLLWQTHKPYFLLILVI
                     NILRGASPVISLLLLQTLINSIGTSWDTGFQTVLWPFIWLTTFTAFNEALSLAQNFISK
                     LFETLLSNRINMLIMEKSISLSLSDFENSQVQDALKRAQNEAGHRPYQILQQILSIVTA
                     SITLFSTAAMLISWKWWAAFLLILIPLTSFYSFLRIGQQEFMLQWNRAPKMRHTWYLSV
                     LLTRDLSFKEIKLFQLGGYLLGKYHELLRQFLAEDKRMIKKRLGMGAIFQAIQQGVNAF
                     MFLMVLWSAFKREILIGHVVSYTQAITLTLVSSQNLTQGILSLCQHNLFLQQLFAFLDL
                     QSSDPAARRIGASEASRATNAPVIQSVEFRNVSFRYPGSSYPALRNVSFTLQRGETLAI
                     VGRNGSGKTTLMKLLTQLYDSFEGEILINGVSIRDMDCEAFRRKVGVVFQDFVQYEMQV
                     RQNIGFGNVAKMEDDAELWKAADRAGIAELVRSMPAGLATQLGRLFDEGQQLSGGQWQR
                     IAIARAFMREAELYVLDEPSSMLDPETELQVFQAFRELVRDRLGIFISHRYSAIRYADH
                     ILMMDQGSVVEQGNHQQLMQQNGMYAELYMMQANAYLDADHERKARSAV"
     gene            5702..7066
                     /gene="penC"
     CDS             5702..7066
                     /codon_start=1
                     /gene="penC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     407.4; E-value: 1.1e-123)"
                     /gene_kind="biosynthetic"
                     /product="lanthionine biosynthesis cyclase"
                     /protein_id="ALR96372.1"
                     /sec_met_domain="LANC_like (E-value: 5.1e-64, bitscore:
                     205.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPKETYEAKWLPLEGAERIERVRSIVMETARRLKDPGDIEQFAL
                     AEENKRIEQGKFYVKWNGLDLARGYSGLCLLFGELDRLEPDGQWDVAGHRYVILMQQAL
                     EQQGIESLSLWSGMAGVVTGIQALSRQGTRYANMLRSLNDFFLRMFPEYMYVVRRNIKE
                     GVLMSDYDVMQGLSGIGRYLLHFKEHPEMRIALTQVLEYMVELCGYKEMQGKQVPGWYI
                     SPDRLISSSDQELYPEGYFNCGVSHGIPGPLALLSLAHLQGVEVEGGQEAIRSIAEWLM
                     RFTIKDEYGLYWPGHVSWQEQTAGAASGSPSREAWCYGGPGVARTLWLAGQALGEPAWR
                     QAAIESFLGTARRPERLWNLHSATFCHGYAGLLQMTQRMIADTGTTELIPLRDRLVDRI
                     VQSYDASAPYGFAELEEGEKLHFAGVLDGAAGVALTLLAVIQDQEPEWDQVFLLK"
     gene            complement(7067..8953)
                     /gene="penD"
     CDS             complement(7147..8832)
                     /codon_start=1
                     /gene="penD"
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="ALR96373.1"
                     /transl_table=11
                     /translation="MSKGKMRSDMIKKGFDRAPHRSLLRAAGVKEEDFDKPFIAVCNSY
                     IDIVPGHVHLQEFGKLVKEAIREAGGVPFEFNTIGVDDGIAMGHIGMRYSLPSREIIAD
                     SLETVVSAHWFDGMVCIPNCDKITPGMMMGALRVNIPTVFVSGGPMKAGKTSDGRSISL
                     SSVFEGVGAYQAGKIDDKGLLELEQYGCPTCGSCSGMFTANSMNCLAEALGLALPGNGT
                     ILAISDDRKEFVKRSAKQLMELIQKDIKPRDIVTMEAIDNAFALDMAMGGSTNTVLHTL
                     ALAHEAGFEYPIERINEVAKRIPHLAKIAPASDYHIEDVHNAGGVSAVLNELFKKPGAL
                     YGDRITVTGKTLRENVAGCEILNTDVIRTLDNPHSEQGGLAVLFGNLAPNGSIIKVGAV
                     DKSVGGYHKGPAICFDSQEAALEGIANGKVKEGHVVVIRYEGPKGGPGMPEMLAPTSQI
                     VGMGLGAKVGLITDGRFSGASRGISIGHISPEAAEGGPIAFVEDGDIIELDLVNRKIEL
                     LVSDEEFERRRANWKGFEPKVKTGYLARYSKLVTSASTGGVMKI"
     CDS             9043..9336
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ALR96374.1"
                     /transl_table=11
                     /translation="MTEFDYLYDGTEETKTRFVCVVGETLRRFDLAITSTNRFYGKKLV
                     TDMQTGRTAVIGPDDLEEEGYLEHAYKLNEEEANELRAFLSEVVGPISFSDI"
     CDS             complement(9408..9725)
                     /codon_start=1
                     /product="pectin degrading protein"
                     /protein_id="ALR96375.1"
                     /transl_table=11
                     /translation="MSNINVWENAEPGVKRKIFEPGAGIMMMEVHFEEGAEGYVHSHPH
                     EQLSYCLKGRLEFTVDGDTKVIAAGETIYIPSGAKHGCRALEASALLDSFTPVREDLIK
                     K"
     CDS             complement(9834..10643)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287:AraC family
                     transcriptional regulator (Score: 88.2; E-value: 8.9e-27)"
                     /gene_kind="regulatory"
                     /product="transcriptional regulator"
                     /protein_id="ALR96376.1"
                     /transl_table=11
                     /translation="MQFKLTTPNMELWRIENTFENIPHAHADFYQITIPVTGTCRLTQE
                     RRVYSISGGHALIQHPGEEHHFSLGPDGGVYIVQVRKQLLVEGWSEQEPELDSRPLFNP
                     EDIKKQMKAWTSELLLRDGPDPLAVEETEDRVLTYIRRVVGKNDSRVLNGRHVDGALAP
                     GMTDILEFIHAHYTERLTMERLASLVYQSKFHFIRSFTQTVGVTPYQYILRLRIEQAKE
                     QLVRTRASVTEISYSLGFSSPSQLYRAFAKATGVTPEQYRKGAAPRR"
ORIGIN
        1 aagggagaag atatttatgg caaacaacaa tttcgatctg gatgtccaag tgaaatcggt
       61 taattcggta aacgcgaatt ttgggcttta cacgtcgcaa tgctatagtt ctcaatgcta
      121 ttcttccaaa tgttattccg attcttgcta ctcctccgac tgctacaccg gcagacatat
      181 gtgcggctac acgcacggct acagctgcta atttcgtcca ttccttccac tttcgccggc
      241 tgccgcaaca atcttcggag aaatgttctc cgttcgaagg gagaaaatct gtatggcaaa
      301 caacgttttc gacctggatg ttgaagtgaa atcggttaat tcggtaaacc aaaacatcgg
      361 cctcttcacg tccacttgct ttagttctca atgcttctct tccaaatgct tcacggatac
      421 ttgcttcagc tcgaactgct ttaccggcag acatcagtgc ggctacaccc acggcagctg
      481 ctaaactttc gcgtgttcct acagcctact tcggaggaac gttcgcgttc ttccgaagta
      541 tctcttacga ctcgataacg gaaggtgaga cgacatgtca gttcaagtgg ccccctctca
      601 accaacgcaa gctcatgcac atccaagcgt ttttcaggcg cttgattttt ttattttgag
      661 gatgccttta ttgccggtgg aactattcca gaacctttct gtcgatccaa atgaggatga
      721 ggcggttagc cgcaagccat ggttcgagaa attggccgcc tacgccgaat tgcctatcat
      781 ccgggaagcc attgccgtat ccagcttgcc gctgcttcag gctttgccca atctaaccaa
      841 ccaggagcat ccgcgcaaac aagatcaggc cgtaaaaggc tttctgcgct atttgctgcg
      901 tatgacctcg cgtccgacgc cttttggttt attttctggg gttacatacg gaaatttcgg
      961 ggagcggtat gcgttcgggc ttcagcccgt aacggcccac accaaacgag cccgccctga
     1021 tatggagtgg ctgctgaaag tgctgtccga cttggagagc cggtttgacg tcgtcaagca
     1081 gttaaaggtc agtctcaatt gtttggctta tcccgtaggc tcccgccttt acctgcccta
     1141 cctgaccaag tacggcgtca agcagaaaag caaaagcact atggagagca cctccgttcg
     1201 ccttactccg gccgtgaagc aggtgatgga gcttacccgt cagcccctcc ccttttgcga
     1261 gttggcactg aagctgcaca gccaattttc cgatacgtca ctcgagcaaa taaaccaatt
     1321 tctgtggcag ctggttcagc aggaaatcat catcacggac ctgcgcccgc ctctcatgac
     1381 cgcttcgcca ttcgattacg tactgcagcg tttagccgat gtcaccggtg tggaagacga
     1441 gaggacgaag ctagaaaccg ccgcagccct catcgcgagc tataaccgta cggaaatcgg
     1501 gcaaggcgag ccattatatc atgagcttgt tgagcatatg aaatccgttg ccgaagcgga
     1561 taatctcctg caagtggatc tggccctctc gatggagacg ttaaccctcc ccaaatccgt
     1621 tgcgcaggag atcgagcgtg tcggcgaagc gctttggcga atctccgggg aacggctgag
     1681 actttcacat ctggaaagct accgcgccga tttcatggag aaatacgggg cttaccgcga
     1741 ggttcccctg ctggagctgc tggacgcaga catcgggctt ggcgctccgg ccggatatga
     1801 atatccccgg ggcaaccgca aggaagacaa caccccttcc ttcgcccagc aaaagaaaaa
     1861 gatgctgctg acccaatggg tcatgacatg cctccagcag gggcggcagg aattggagct
     1921 gaccgacaac atggtgatgc agctcgaacc tgtgccgatg gaggaaaagt acgcgccacg
     1981 gtcgttggaa ttggtttttg gccttgccgc ccgatccggc caagcggtgg aggagggtga
     2041 ctttctcctg attgccggag gaacgacggg cacctacggt gcaggcaaat ccgtcggccg
     2101 gtttgtcgat ttatttgatt cctcctttgt ggacaagctc aagcaaattg accgattgga
     2161 gcaaaagctt cagcccgaag cgatcctcgc cgagctcgtt tatttcccag gagacggccg
     2221 cgcggctaat gtcgctctga gcacgaacat tcgcgaatac gaaatcgaca tcggcaccaa
     2281 ttcggccaaa gatccggagc atacgattcc gctctccgat ctggtcgtcg gtatttctgc
     2341 gagcggtttt tatttgcggt ctctggcaaa aaaccgaaga gttattccgg tgacaaacca
     2401 catgctgaat acgtcgaatt cgcctaacgt ctaccggttt ttgcgtgaaa tatcgatgga
     2461 aaaccagcgc aacattgatt tttttgagtg gaacgagtta agaactcttc ctttcttgcc
     2521 gcgggtaagg tacagcaaaa cgatcattgc ggcggcctgc tggaagttta acggggacct
     2581 gccacctttt cgcgggaaca tgacggagac gcagtggcgc caagcgttcg aggcgtaccg
     2641 gaagcaatgg aatgttccgc gctttgttta tttgacccaa tcggataacc gcttgctgct
     2701 ggatctcgac tccccgcttc atcgggacga agtgtatcgg gattatacga agcttcagcc
     2761 tggggggcaa atcgtcttaa ccgaaaccgg ctttgcgttt gagcatctgc ccgagagcgc
     2821 agacgggcac tacgtgaccg agtttgtctt tcctctcgtc aaacgggatc tgccgcaagc
     2881 ttccacgcag ccggatctcg gcggaactct cgcctccccc tccatccgcc tcgcggagag
     2941 attgaaaatg ccgggcagcg aatggctgta tctgaaatgg tacggtgcgg atcatcgcgc
     3001 cgacgctttt atcggaggac ctctgctgga gttttgccgc caggcggagg agcaaggatg
     3061 ggctgccggc agcttcttta tgcgctatgc cgatcccgat ccgcatcttc gctggcgttt
     3121 ctgcggccat cccgagcagc ttcacaccgt gctgctgccg cagctgtacc ggtttacgcg
     3181 gcagtgcgtg gaagacggac tcttgtcccg cctggtgatc gatacttacg atccggaaat
     3241 cgagcggtac gggggccgga cgttgatagg gacggcggaa cggctgttct gtgtcgacag
     3301 ccgggtcgtg gccgaatgga ttcacgccag acaatcggga cacctcagcc tggacccgca
     3361 cttgctggct gtcatcagcg tcattgacat gatggaacga ttcgggctgt ccttcttcga
     3421 gcaattcgac tggcttaaca cgatggtaca gcataaggag catttggagc tgttccgtca
     3481 gcaccggccg tttcttatga aattcgggga tgccagagga aactttgccc agcttcgcca
     3541 tctccctgac ggagcggtca tcctgccgat gctccaagcc cgcggcgagg cgatagcccg
     3601 gtacgtcgag ggaatccggc tgtgccaagc ggaaggaacc ttgaccaata ctcaagagga
     3661 tctgatgcaa agcgtgatcc atatgcatct aaaccggctg ttcggcatcc aacgagaaga
     3721 agagcagaag gtgttgactc tggtcagaca tacgctatac agcttgcagc atgtgaggag
     3781 gaatcagcca tgagctcggc tgcggaagaa gcatccctaa ccgtaaatct aacgaatatt
     3841 gtgcaatcct ttcgctattg gccccgcgtg ttccaattgc tgtggcagac gcacaaaccg
     3901 tatttcttgc ttattctagt gattaacatt ctgcgcggcg cctcgccggt catctcgctg
     3961 ctgctgctcc aaaccctgat caacagcata ggcacctctt gggatacggg ttttcagacc
     4021 gtgctttggc cctttatttg gctcactacg tttaccgcat ttaatgaagc gctgtcgctg
     4081 gctcaaaatt tcatcagcaa attatttgaa acgctgctca gcaatcggat caacatgctc
     4141 attatggaga agtcgatttc cctttcgctt agcgattttg agaactccca ggtgcaggac
     4201 gccctgaaac gcgcccagaa cgaggccggt catcggcctt atcaaatcct gcagcagatt
     4261 ttgtccattg ttacggcttc cattacgtta ttctcgaccg cggctatgct catttcgtgg
     4321 aaatggtggg cggcttttct cctgatcctc attcccttga cctccttcta ttcgtttctg
     4381 aggatcgggc agcaggagtt tatgcttcaa tggaaccggg ccccgaaaat gcggcatacc
     4441 tggtatttgt ccgtgctgct gacaagggat ctctctttca aggagatcaa gctgtttcaa
     4501 ctgggcggtt atttgctcgg caaatatcac gagctgctgc gtcaattttt ggcggaagac
     4561 aaacgcatga tcaaaaagcg gctcggcatg ggagcgattt ttcaagcgat ccagcaaggg
     4621 gtcaacgctt tcatgtttct catggtctta tggtccgcct ttaagcgcga aattttgatc
     4681 ggccatgtgg tcagctatac ccaagcgatt acgttaaccc tcgtttcgtc gcaaaatctg
     4741 acgcagggca tcctttcctt atgccagcac aatttatttt tacagcagtt gttcgctttc
     4801 ctggatctgc agtcctccga tcctgccgcc agaagaatcg gagcttcgga ggcttcccgt
     4861 gcgacgaatg cgcctgtcat ccaatcggtg gaattccgca atgtgtcttt ccgttatccc
     4921 ggcagcagtt atccggctct gcgtaatgtc agcttcaccc tgcagcgcgg agaaacactc
     4981 gcgattgtag ggcgcaacgg gtccgggaag acaacgctga tgaagctgct gacgcagctg
     5041 tacgactcct ttgaaggaga gattctcatc aacggagtct cgatccgcga tatggactgc
     5101 gaagcgtttc gccgcaaggt cggcgtcgtg ttccaggatt ttgtccaata tgagatgcag
     5161 gtccggcaaa atatcggttt tggcaacgtc gccaaaatgg aggacgacgc cgaattgtgg
     5221 aaggcggcgg atcgggcggg catcgccgag ttggtccgat cgatgcccgc aggccttgcc
     5281 acgcagctcg gacgcttgtt tgacgaaggg cagcagctct ccggtggaca gtggcagcgg
     5341 attgccatcg cccgagcctt catgcgcgaa gccgagctct atgtgctgga cgagcccagc
     5401 tccatgctcg acccggaaac cgagctgcag gtgttccagg cgtttcggga actggtccgc
     5461 gatcggttgg gcatcttcat ttctcaccgc tactccgcca tccggtacgc cgaccatatc
     5521 ctcatgatgg atcaaggctc cgtggtcgag cagggcaatc atcaacagct catgcagcag
     5581 aacgggatgt atgcggagct ctatatgatg caagcgaatg cttatttgga cgccgatcat
     5641 gagcgcaaag cgcgttcggc cgtttgattg cggcaaccat gatccgaagg gagaaatcgg
     5701 aatgaacccg aaagaaactt acgaggccaa gtggctgccg cttgaaggcg ccgaacgaat
     5761 cgaacgggtc cgctcgattg tgatggagac ggcccggcga ttgaaggacc cgggagacat
     5821 cgagcaattc gctctcgcag aagaaaataa acggatagag caaggaaaat tttacgtgaa
     5881 gtggaacggg ctggaccttg cgcgggggta ttcgggttta tgcctgttgt tcggcgagct
     5941 ggatcggcta gagcccgatg gacaatggga cgtcgcaggt caccgctatg ttattctcat
     6001 gcagcaagct ttggaacagc aaggaatcga gtccttatcc ctatggagcg gtatggccgg
     6061 agtcgttacg ggcatccagg ctttgtcgcg gcagggcacc cggtacgcga atatgcttcg
     6121 gtccctcaac gactttttcc tgcgcatgtt ccccgagtac atgtatgttg tccggagaaa
     6181 tataaaagaa ggcgtgctca tgtccgatta cgacgtgatg cagggcttaa gcggcatagg
     6241 ccgttatttg ctgcacttta aagagcatcc cgagatgcgc attgcgctta cgcaggtgct
     6301 tgagtatatg gttgagctgt gcgggtacaa ggagatgcaa ggtaaacaag ttccgggatg
     6361 gtatatttcg cccgaccggc ttatttcctc ctccgatcag gagttatacc ccgagggtta
     6421 cttcaactgc ggagtttccc acggcattcc gggtcctttg gccctgctct cccttgcgca
     6481 cctgcaaggc gtagaagtcg agggcgggca ggaagccatc cgctccatcg ccgagtggtt
     6541 gatgcggttt acgatcaagg atgagtacgg cctctattgg cccggtcatg tatcgtggca
     6601 agagcagaca gcgggcgcgg caagcggctc gccgtcccgc gaagcttggt gctatggcgg
     6661 gcccggcgtc gcacgcacgc tatggctcgc ggggcaagcg ctcggagaac cggcgtggag
     6721 gcaagcggcg attgaatcgt tccttggaac ggcccggcgg cccgaacggc tgtggaactt
     6781 gcattccgcc accttttgcc acggctatgc cggtctcctg caaatgacgc agcggatgat
     6841 cgcagacacg gggaccacgg agctcattcc tttacgcgac cgtctcgttg atcggattgt
     6901 tcagtcctat gatgcgagtg cgccttacgg ctttgcagag ctggaagaag gcgaaaagct
     6961 gcatttcgcc ggagtgctgg acggggcagc aggcgttgcg ctcacgctgc ttgcggtcat
     7021 tcaagatcag gagccggagt gggatcaggt tttcctattg aagtaaggtt agttgttacg
     7081 taaaaaagcc ttcggtgccg gatcgtttcg ttccggacgc gaaggctttt ttttgcaggg
     7141 gaattactag attttcatga ctccaccggt gctggccgag gttacgagct tggaatagcg
     7201 ggccagataa cccgttttca ccttcggctc gaagcctttc cagttcgcac ggcggcgttc
     7261 gaattcctcg tcgctgacca gcagctcgat tttacggtta acgagatcga gctcgatgat
     7321 atcgccgtcc tcgacgaatg cgatcggacc gccttccgcc gcttccggcg agatgtggcc
     7381 gatgctgata ccgcgtgatg cgccggagaa acggccgtcc gtaatgagac cgactttcgc
     7441 gccgaggccc attccgacga tctgcgaggt tggggcgagc atttccggca tgccgggacc
     7501 gcctttcgga ccttcgtaac ggataacgac gacatggcct tcttttactt tgccgttggc
     7561 gatgccttcc agcgccgctt cctgcgaatc gaagcagatg gccggccctt tgtgatagcc
     7621 gcccacggac ttgtccaccg cgccgacctt gatgatcgag ccgttcggtg ccaggttgcc
     7681 gaacagcacc gccaagccgc cttgctccga atgcgggtta tccagcgtac ggatgacgtc
     7741 cgtgttcaaa atctcgcagc cggcgacgtt ttcgcgcagc gttttaccgg tcacggtgat
     7801 gcggtcgccg tacagcgcgc ccggcttttt gaacagttcg ttcaggacgg cgctcacgcc
     7861 tccggcgttg tgaacgtcct cgatatgata atcggaggcc ggagcgattt tggccaagtg
     7921 cgggatgcgc ttcgccactt cattgatccg ctcgatcggg tattcgaagc ccgcttcgtg
     7981 cgccagagcg agtgtgtgca gcacagtatt ggtcgagccg cccatcgcca tatccagcgc
     8041 gaacgcattg tcgatcgctt ccatcgtcac gatatcgcgc ggcttgatat ccttttgaat
     8101 caactccatg agctgcttcg cggaacgctt cacgaattcc ttacggtcat cggaaatcgc
     8161 caaaatggtg ccgttgcccg ggagcgccag accgagagct tccgccagac agttcatcga
     8221 gttggccgtg aacatgccgg agcaggagcc gcaggtcgga cagccgtatt gctcaagctc
     8281 aagcagacct ttatcgtcga ttttgccggc ttggtaagcg cccacgcctt cgaatacgga
     8341 agacagtgaa atcgaacgtc cgtcgctcgt ttttccggct ttcatcggac cgccgctgac
     8401 gaataccgtc ggaatgttga cgcgcagcgc gcccatcatc atgcccggcg taattttgtc
     8461 gcagttcggg atgcagacca tgccgtcgaa ccagtgcgcg gacacaaccg tttcgagaga
     8521 atccgcaata atctcgcggc tcggcaagga atagcgcatg ccgatatgcc ccatggcaat
     8581 gccgtcgtct acgccgatcg tgttaaattc aaacggcacg ccgcccgctt cgcgaatcgc
     8641 ttctttcacg agcttgccga attcctgcag gtgaacgtga cccggaacga tgtcgatata
     8701 cgagttgcaa accgcgataa acggcttgtc gaagtcctcc tctttgacgc cggcggcgcg
     8761 cagcaagctg cggtgcggag cgcggtcaaa gccctttttg atcatgtcgc ttctcatttt
     8821 tcccttggac attcgcttaa agcctccgtt tccatcccta tgaaaataga attctacgat
     8881 ttaacgcttc actcgattgt atcacgtttc cccggaattg aaaacgtgaa ccggattgga
     8941 aaaaaggcgg tttccccggc tgacgcgccg tttttgcggc ctggtcggca aatctgttat
     9001 aattcggtta gcttcaggaa tttaagtatc ggaggatgcg tcatgacgga gtttgattat
     9061 ttgtacgacg gtacggagga aacgaaaacc cgcttcgtat gcgtagtcgg cgaaacgctg
     9121 cggcggttcg atctggcgat tacgagtacg aatcgttttt atggaaaaaa attagtcacc
     9181 gacatgcaga cgggacggac ggcggtcatc ggaccggacg atctggagga agaaggatat
     9241 ttggagcatg cctataagct taacgaagaa gaggccaacg agctgcgggc gtttctgagc
     9301 gaggttgtcg ggccgatcag cttctcggac atttaatgca cttggaacct gcagataaaa
     9361 aaagccgccg gcaccgcaca atacggcatc cgggcggctt ttccccgtta tttcttaatc
     9421 agatcctcac gcaccggcgt gaacgagtcg agcagcgcgc tcgcctccag cgcccggcag
     9481 ccgtgcttcg ccccgctcgg gatatagatc gtctctcccg ccgcgatcac tttcgtatcc
     9541 ccgtccaccg taaattcaag ccggcccttc aagcagtacg acagctgctc gtgcgggtgg
     9601 ctgtgcacgt atccttcggc cccttcctcg aaatgcactt ccatcatcat gatgccggct
     9661 cccggctcga atattttccg tttcacgccg ggctcggcgt tttcccatac gttgatgttg
     9721 ctcatgcgtc gtcccctctt cccgatcctg atctttcgat tatatcgcag gggcgtacga
     9781 attcgaaggg agcttccccg gatgaagtag gctttttttc aggtgcggac gtcttagcgc
     9841 cggggggcag ctcctttgcg gtactgctcc ggagtgacgc ctgtcgcttt ggcaaacgcg
     9901 cggtacagtt gacttgggct cgaaaatccc agactgtagc tgatctccgt gacgctggct
     9961 cgcgtcctca ctaactgctc cttcgcctgt tcgatccgca ggcgcaaaat atattgataa
    10021 ggcgtcacgc ccaccgtctg tgtaaaagag cggataaagt gaaacttgct ctgataaacg
    10081 agcgaagcga gcctctccat cgtgagccgt tccgtataat gcgcatgaat aaactccagg
    10141 atatctgtca tccccggcgc gagcgcccca tcgacatgtc gtccgttcag tacacgtgaa
    10201 tcgtttttcc ccaccacgcg ccgtatataa gtcagcaccc ggtcttcggt ttcttctacc
    10261 gcgagcggat cgggaccgtc gcgaagcagc agctcggaag tccaggcctt catttgtttt
    10321 ttaatgtctt ccgggttaaa aagcggccgc gaatccagct ccggctcctg ttccgaccag
    10381 ccttcaacaa gcagctgctt ccggacctgc acgatatata cgcccccatc cgggccgagc
    10441 gaaaaatgat gctcctcccc cggatgctgg atcaaagcgt ggcccccgct gatcgaatac
    10501 actctccgct cctgcgtcaa acggcaagtg ccggtgacgg gaatggtgat ttggtaaaaa
    10561 tcggcatggg cgtgagggat attctcaaac gtattctcga tgcgccatag ttccatattc
    10621 ggcgtcgtta gtttaaattg cat
//
