BGC0001290 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001290 (Aspergillus nidulans FGSC A4)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	72,532	High	emericellamide A/emericellamide B	NRPS:Type I

BGC0001290 - Region 1 - NRPS,T1PKS

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Location: 1 - 72,532 nt. (total: 72,532 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 72,533
Label		BGC0001290

Tailoring functions

Ligases: ligase synthase

ANIA_02545: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ANIA_02547: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ANIA_02549: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001290.4	1.42	NRPS	emericellamide A, emericellamide B	Aspergillus nidulans FGSC A4
BGC0002191.2	0.90	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001275.3	0.65	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.65	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001268.4	0.61	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.60	PKS	ACT-Toxin II	Alternaria alternata
BGC0000107.3	0.60	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002259.2	0.60	NRPS, PKS	beauveriolide I, beauveriolide III	Cordyceps militaris CM01

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001290.4	0.97	NRPS	emericellamide A, emericellamide B	Aspergillus nidulans FGSC A4
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.71	NRPS	AM-toxin	Alternaria alternata
BGC0001240.4	0.70	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 72532:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Gly - Val - Leu - Ala - Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 60852:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Gly - Val - Leu - Ala - Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 24637 - 72532:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Gly - Val - Leu - Ala - Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ANIA_02545: Gly - Val - Leu - Ala - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (1002..1394): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DIQGVLAMQK
Nearest Stachelhaus code(s):: Gly DIQGVLAMQK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2535..2930): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASQIGGIYK
Nearest Stachelhaus code(s):: Val DASQIGGIYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3619..4018): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIHFVGAIAK
Nearest Stachelhaus code(s):: Leu DIHFVGAIAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4731..5133): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DLLVVAGILK
Nearest Stachelhaus code(s):: Ala DLLVVAGILK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5823..6216): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DIAILVAILK
Nearest Stachelhaus code(s):: Ala DIAILVAILK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ANIA_02547: pk

PKS_AT (586..902): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 58.3%; inactive: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 71.8
Methylmalonyl-CoA: 65.1
Malonyl-CoA: 50.2
Isobutyryl-CoA: 45.6
Ethylmalonyl-CoA: 34.2
fatty_acid: 26.3
Propionyl-CoA: 22.5
2-Methylbutyryl-CoA: 21.2
3-Methylbutyryl-CoA: 20.9
inactive: 17.9
Benzoyl-CoA: 11.0
trans-1,2-CPDA: 10.2
Acetyl-CoA: 9.0
CHC-CoA: 0.0

ANIA_02549: X

AMP-binding (48..465): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GLGYVGAVVK
Nearest Stachelhaus code(s):: nohBza GLGLVGAIIK (41% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor