LOCUS       BGC0001291              9016 bp    DNA     linear   BCT 03-MAY-2018
DEFINITION  Enterococcus faecium DNA contains enkB, enkB1, enkB2, enkB3, enkB4
            genes for enterocin NKR-5-3B precursor peptide, proteins involved in
            secretion and immunity/maturation, immunity protein, strain:
            NKR-5-3.
ACCESSION   BGC0001291
VERSION     BGC0001291
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:16:00
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9016
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..711
                     /codon_start=1
                     /note="arsenic-resistance protein: orf7"
                     /product="hypothetical protein"
                     /protein_id="BAT21389.1"
                     /transl_table=11
                     /translation="MIGIARCIAMVIVWSELAQGDSEYTAGLVAFNSIFQIVFYSLFTY
                     IFVTVLPTWLGLETHSVNITMGEIAKSVFIYLGIPFIAGISSRFLLLKFKGKTWFETKF
                     IPKISPITLIALLLTIVIMFSVKGEKVIELPLDVVRIAIPLFLYFIIMFFVSFFISNKM
                     GTSYPVAASLSFTAASNNFELAIAVAVDVFGINSGEAFAAVIGPLVEVPVLIALVNVAL
                     KFKQKYFTKEGVSH"
     source          1..9016
                     /db_xref="taxon:1352"
                     /mol_type="genomic DNA"
                     /organism="Enterococcus faecium"
                     /strain="NKR-5-3"
     protocluster    1..9016
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[8075:8648]"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      8076..8648
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     cand_cluster    1..9016
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="neighbouring"
                     /product="cyclic-lactone-autoinducer"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..9016
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001291"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9016
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /contig_edge="True"
                     /product="cyclic-lactone-autoinducer"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    1..8767
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3503:3767]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      3504..3767
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
     cand_cluster    1..8767
                     /candidate_cluster_number="2"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="2"
                     /tool="antismash"
     CDS             721..1803
                     /codon_start=1
                     /note="arsenic-resistance protein complex: orf8"
                     /product="hypothetical protein"
                     /protein_id="BAT21390.1"
                     /transl_table=11
                     /translation="MSAENKKQGLGIFEKYLTLWVILCMAVGVLIGKFIPAVADTLSKF
                     EYYQVSIPTAILIWLMIYPMMLKIDFTSIARAAKKPKGLIITCVTNWLIKPFTMYAISA
                     FFFYVVFKNLIPVDLAKEYVAGAVILGAAPCTAMVFVWSYLTKGDAGYTLVQVAINDLI
                     ILFLFAPIVAFLLGVDNVAVPMGTLLLSTLLFVVVPLLLGFFTRVIIIKNKGLAYFENV
                     FLKKFDSVTIMGLLLTLIIIFSFQGEKILANPMHILLIAIPLIIQTFLIFTIAYGWAKL
                     WKLPHDIASPAGMIGASNFFELAVAVAISLFGLQSGATLATVVGVLVEVPVMLLLVKIS
                     NRIKNWFIQNLSELSDIGGK"
     CDS             1806..2213
                     /codon_start=1
                     /note="arsenate-reductase: orf9"
                     /product="hypothetical protein"
                     /protein_id="BAT21391.1"
                     /transl_table=11
                     /translation="MRKIYFLCTGNSCRSQIAEGYGKAILPTAEFEIKSAGIEQHGLNP
                     RAVQVMAEEGIDISTQTSDLIDMDYFNQADLIITLCGDAKDKCPVIPKGIEHAHWDLED
                     PAKATGTEEEIINKFREIRDDIKSRVANLLD"
     gene            3504..3767
                     /gene="enkB"
     CDS             3504..3767
                     /codon_start=1
                     /gene="enkB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /product="enterocin NKR-5-3B precursor peptide"
                     /protein_id="BAT21392.1"
                     /sec_met_domain="TIGR03651 (E-value: 6.6e-17, bitscore:
                     51.1, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKNLLLVLPIVGIVGLFVGAPMLTANLGISSYAAKKVIDIINTG
                     SAVATIIALVTAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
     misc_feature    3504..6527
                     /note="genes involved in the biosynthesis of a circular
                     bacteriocin, enterocin NKR-5-3B"
     gene            3739..4923
                     /gene="enkB1"
     CDS             3739..4923
                     /codon_start=1
                     /gene="enkB1"
                     /note="membrane protein"
                     /product="protein involved in secretion and immunity"
                     /protein_id="BAT21393.1"
                     /transl_table=11
                     /translation="MELNMQQLGKNVLKATIKSPLIFPKKLLLAQYRKRINWPWSTKSL
                     FFVASSVYILSALLLGFFDSVFSNVIFSFSVFVLLLNYNLPLTSDVFNMKKFRVVLANN
                     HGKKRFLLWLILYYNPFILVGVLFFASSVCASFIQGNFIHSIDLFFLFILSIQVLIAEI
                     VMSRRGKAVTTLQLVFFLATTILQMNLIIKYVLYLLLSIIGYLFFRNPNDFYFQKVTKS
                     KNSTSRSVLKTMLYNVIFSDKKQKIFMLVPGVIAPLIAHKLGASFSPAITFILMLLVQY
                     EIVIDNQLEDFEVPLAKIKALKSAKISFFKRFVSTLYFKLSMFTIFIILIANYFYSRNF
                     IEFIIPSIVETIYIPLMSMYYLFFLERELMTRSHINSVLREFLPITIIFTFFIF"
     gene            4925..5443
                     /gene="enkB2"
     CDS             4925..5443
                     /codon_start=1
                     /gene="enkB2"
                     /note="membrane protein"
                     /product="protein involved in maturation"
                     /protein_id="BAT21394.1"
                     /transl_table=11
                     /translation="MGGILIDTLDVNINIKKRVILFFVVSIFIFLILFLFYQYSGILQT
                     EELVSTPPVKGIEYVEAIFVNNLLNYLQYLFFPVAPALIIKDDILLSVPIAQSAINFGV
                     IQTLKNLFPHGFLEIPNILCFQFLSITMFYQLFFKGWKTLVPTFMKLRKVYLASLLVIL
                     IAAIVEGVF"
     gene            5443..6033
                     /gene="enkB3"
     CDS             5443..6033
                     /codon_start=1
                     /gene="enkB3"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 84.5; E-value:
                     1.6e-25)"
                     /gene_kind="transport"
                     /note="ATP-binding protein, soluble protein"
                     /product="protein involved in secretion and immunity"
                     /protein_id="BAT21395.1"
                     /transl_table=11
                     /translation="MIEIKSLKKKYTNNQILNELNYTLKDEIYVLYGKNGVGKTTLMNL
                     LTGIESPDSGEIKNESGLSSLYLEAVGIGMSDMSIRDNIKLLYWLYGERCTDEILELLS
                     ETLYSKEQLDTEYALASLGMKLKVGLSLLYAAKEWGLIVLDETLSGIDPQSKKVILEQL
                     KTKKCPVIIVSHNFDNINSYKKIELTKEGIVNQ"
     gene            6030..6527
                     /gene="enkB4"
     CDS             6030..6527
                     /codon_start=1
                     /gene="enkB4"
                     /note="membrane protein"
                     /product="immunity protein"
                     /protein_id="BAT21396.1"
                     /transl_table=11
                     /translation="MKDNIFFKVIMSVFYSIVLMLMLIELITKPIFKPVFDIAVHETGI
                     PASKLLIICGIAIFLFGFVLLFFQTGIYFMGLKLFNTSKENLTKIFPSVTLSMSIVGVI
                     FLFVSMFIDVTNSLVLLTTPAIVLLLNSIIYYILSKDRKGFLIILVISMVMYAMNGLVN
                     RI"
     CDS             complement(6614..7924)
                     /codon_start=1
                     /note="histidine kinases: orf5"
                     /product="hypothetical protein"
                     /protein_id="BAT21397.1"
                     /transl_table=11
                     /translation="MIIFYLIGAFLSSYFVTSLYFFLLGSSQTKFKHKAISAILIFIFL
                     FVIGSSSFPQFQYIFVIAILVLIGFYLKKQEKKTLVIFLSIFTVLISAFLNFTEQSINT
                     IIFRNYSKEELIVSVLNIIFLTFNIFIIRVAKPDKVASMRDYLRPNKYLIPMILINIFL
                     LVLFSELTTSYAPIEFIQDLFLNDKGLMYLFFSLGLFTLLVLIQFGKHHTENEQNILLL
                     RNLSDYNNEIERLNDDLAMFRHDYLNILHTLRIAIANEDLAQIKDTYEHVLAPTEKFLL
                     NISKQNTALSKIKNIEIKSLVSYKYEYAKRRNLSVFLSINQPIFFDQTVDLVMLVRILS
                     ILLDNAIENTIPTEDKSLWISINKDINSTNFIIENSFEGTFPEMNKKKERYTTKSDKNN
                     HGLGLLYVNQITKENDNYDLETTIENQIFSQKLTIKI"
     CDS             complement(8076..8648)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /note="response regulator: orf6"
                     /product="hypothetical protein"
                     /protein_id="BAT21398.1"
                     /sec_met_domain="AgrB (E-value: 9.9e-27, bitscore: 82.9,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVNYIAQKIFLISVDDNDERSIYTRYYIECYLVGLQKLCEIIILA
                     ALFKTFKETIIVGFLMTVTRKYSKGWHSKSKLGCSVLNALFYVVIPYFIGKMNFQISYF
                     ALAIIGIVVLVLTAIYAPQFNAAEMKTTKEKNLNTKVKAISVNLGIFIISIFCPNEIQN
                     LLVYTLVLQSLMIYPPLKKFITGDAYK"
ORIGIN
        1 atgattggta ttgctcgatg tattgcgatg gtcattgtct ggagtgaact tgctcaaggc
       61 gatagtgagt acacggcagg cttagttgcc tttaactcga tcttccaaat tgttttctat
      121 tcgctattta cctatatatt tgtgactgtt ttgccgacat ggttaggttt agaaacacat
      181 tcggtaaata taactatggg tgaaattgcc aaaagtgtct ttatttatct aggaattcct
      241 tttattgctg ggattagttc aagatttctt ttacttaaat tcaaagggaa aacgtggttt
      301 gaaacaaaat tcattcctaa aattagtcca attacattaa ttgctttgtt acttacaatc
      361 gtgattatgt tttctgttaa aggggaaaaa gtcattgaat tacctcttga tgttgtaaga
      421 attgccattc cattattttt atactttatt attatgtttt tcgtttcatt cttcatttct
      481 aataaaatgg gcacgagtta tccagtcgca gcttcgctct cttttaccgc agcaagtaat
      541 aactttgaat tagcgattgc tgtggcagta gacgtctttg ggattaattc gggggaagct
      601 tttgcagccg ttatcggtcc actagttgaa gtgcccgtac tcatcgcatt agtaaatgtt
      661 gctttgaaat ttaagcaaaa atattttacg aaagaaggcg taagccacta ggaggaaaaa
      721 atgagcgctg aaaataaaaa acaaggatta gggatttttg aaaagtatct gactttatgg
      781 gttattttat gtatggcagt aggtgtgttg attgggaagt ttattccagc tgtggcagac
      841 acattaagta agtttgagta ttatcaagtc tctattccaa ccgcgatatt aatctggtta
      901 atgatttatc cgatgatgtt gaaaattgat tttactagca ttgctcgtgc agcaaagaaa
      961 cccaaaggac taattatcac ctgtgtaacc aattggttga ttaaaccatt tactatgtat
     1021 gccatttcag ccttcttctt ttatgttgtc ttcaaaaatc taattcccgt ggatctagct
     1081 aaggaatatg ttgccggagc agtcattctc ggcgcagcac catgtaccgc tatggttttt
     1141 gtctggagtt atttaacaaa gggagacgca ggttatacct tggttcaagt agctatcaat
     1201 gatttgatta ttctatttct attcgcacct atcgtagcgt tcttactagg tgttgacaat
     1261 gtggccgttc caatggggac actcttacta tcaacgttgc tctttgtggt tgtaccgcta
     1321 ttattaggct tcttcacacg cgtgataatt ataaaaaata aaggcttagc gtattttgaa
     1381 aatgtcttct taaagaaatt tgattcggtt accattatgg ggttattatt aacattgatt
     1441 attatcttct ctttccaagg agaaaagatt ttagctaacc ctatgcatat tttacttatc
     1501 gcgattccat taattatcca aacctttctt atttttacaa tcgcttatgg atgggctaag
     1561 ctttggaagc taccgcatga tattgcatca ccagcaggta tgatcggcgc aagtaacttt
     1621 tttgaattag ccgttgcagt agctatttca ttatttgggt tacaatccgg cgctacgtta
     1681 gcgactgtag ttggcgtttt agtcgaagta ccagtcatgt tactgttagt taaaatatct
     1741 aatcgcataa aaaattggtt cattcaaaat ctatcagaat tgtctgatat cggagggaaa
     1801 taacaatgag aaaaatttac tttttatgta caggcaattc ttgtcgtagt caaattgcag
     1861 aaggttatgg aaaagctatt ttgccaactg ctgaatttga aattaaaagt gccggcattg
     1921 aacaacatgg gttaaatcct agagctgttc aagtaatggc tgaggaaggg atagatatct
     1981 ctacgcaaac atctgatttg atcgatatgg attattttaa tcaggcagac ctaattatta
     2041 cgctatgtgg tgatgccaaa gacaaatgtc cagtaattcc aaaagggatt gagcatgctc
     2101 attgggattt ggaagatcca gccaaagcaa ctggaacaga agaagaaatc attaataaat
     2161 tcagagaaat tagagatgat ataaaaagtc gtgtagcgaa tttgctagat taagtagcaa
     2221 ctgtcatcta tctcggtaaa agataaaaag acgtaaaatg tccttttgat aaattggaaa
     2281 attaaagtaa aagccgtgcg aagtgaaaca taaatcttcg atggctctta ctattttaag
     2341 agaaaagaat ctgcttttgg cgtaaaattg gatttatcta tctgacatac tgacgagtat
     2401 tttttgaccc tatatttgac cttctcaatc acatttaagt aggtgtgacc ggcttataca
     2461 attattcgag aatagctaat tctggccttg aaacccctat tagacagcta tccagccatc
     2521 aatacggtct caaataaata agaggggagg aataactcta aaaaagattg ataaactagt
     2581 aagaaaattg tattcaacta ccattcatct ttggttcaaa ttggttcaag taatatcaat
     2641 attaatccta ttttatccat atcaaactgt tttttagatg tttgtaatgt tgatataatt
     2701 gtatttctat tgatagaagt acttgtaata agtacgagtc acatctcaaa acgttaatat
     2761 atcttatata tcaacatttc agacactttc caatttttgg aaggtgtctt ttttttattt
     2821 attacgactt cgaatctata ggttagaggc aacttgcttc taattatcat ctttttcgtc
     2881 taatcagtga aaaactagtt ctgctcgtcc aacaaaaatt gaggatctaa caaatgagat
     2941 acgttatttc acgaaaattg cagaacagtg agcctctttc caaaaacttt catcttcgta
     3001 gactgaactg tgcaattctc actacacggt ttttgtacat ttttgaacta acttgagaaa
     3061 ttttccatct ttagaatttt gtaaagtctt gttgagccaa gcgtgatctg tttttcataa
     3121 aaaagtcttt ttttaaagaa aacaaataat agtttatgga taagtacaca attaagcatg
     3181 agatcatcca atgagaaagg atgagattga gtctaaaagc acctagtacg tgctaatagg
     3241 ctatcgtgtt ttttagtacg gctgtagatt gctagaaatt aggatacttt accaattagg
     3301 cactttaaac tgttatttag gtaatttcta aaacatggaa cttttcaacg aataaactaa
     3361 aaatatctta ttaattgatc aatataagaa aatagcttca aggagatgat gaacctgctt
     3421 tctcacctat ttatggatag ctaggtgatt tattcagtgg tggtagtaac agtttttaaa
     3481 caataaaaca tggaggaagt attatgaaaa agaatttatt attagtttta ccaattgtag
     3541 gtatcgtagg attatttgtg ggtgcaccaa tgctaactgc taaccttggg atctcttctt
     3601 atgcagcaaa aaaagttatt gacataatta atactggtag cgcagtcgct acaatcattg
     3661 ctcttgtcac tgctgttgtt ggaggcggct tgattacagc tgggattgtt gctacagcga
     3721 aatcattgat taaaaaatat ggagctaaat atgcagcagc ttggtaaaaa cgtattaaag
     3781 gcgactatca agtcgccttt aattttccca aaaaaacttc ttttagcgca atatcggaaa
     3841 cgaataaatt ggccatggtc aacaaaatca ttattttttg tcgcttcgag tgtttacatt
     3901 ctttcagcac tactattggg attttttgat tccgtttttt ctaatgtcat attttcattc
     3961 agtgttttcg tcttgctact aaattataat ttgcctttga ctagtgatgt cttcaacatg
     4021 aaaaaattca gggtagtatt agcgaataat catggaaaaa agagatttct tttatggtta
     4081 atcctttatt ataatccttt tattttagta ggcgttctgt tttttgcttc ttctgtatgt
     4141 gctagtttta ttcaaggtaa ttttattcat tcgattgatc tattttttct attcatttta
     4201 tcaattcaag tgttgattgc agaaatagtt atgtcaagaa gaggaaaggc agtcacaaca
     4261 cttcaattag tctttttttt agctacaact attttgcaga tgaatcttat tattaaatat
     4321 gtgctttatc ttttattgtc aataattggt tatttgtttt ttaggaatcc taacgatttt
     4381 tattttcaaa aagtaactaa atctaaaaat agtactagtc gttctgtctt aaaaacgatg
     4441 ttatacaacg taatattcag tgataagaag caaaagatat tcatgctggt gccaggggtg
     4501 attgcgccat taatcgctca taaattaggt gcttcgtttt cccctgcaat tacttttatt
     4561 ttaatgttac ttgtccaata cgaaattgta attgataatc agctagaaga tttcgaagta
     4621 ccattagcaa aaattaaagc tctaaaatct gcaaaaattt cattttttaa aagatttgtt
     4681 tcaactctat attttaaact gtcaatgttc actattttta ttattttgat tgcaaattat
     4741 ttttactctc gaaattttat tgagtttatt attccgagta tcgttgagac tatctacata
     4801 ccgttaatgt ccatgtatta tttgttcttt ttggaaagag aattgatgac aagatctcat
     4861 ataaattcag ttttgcgtga atttttgccg attacgatta tcttcacttt ctttattttt
     4921 taggatggga ggaattctta tagatacttt agatgtaaac attaatataa agaaacgggt
     4981 aatactattt tttgtggttt caatttttat ttttctaatt ctattcttgt tttaccaata
     5041 tagtggaatt ttacaaactg aagagttagt ctcaacccct cctgtaaaag ggatagaata
     5101 cgttgaggcc atttttgtca acaatctact aaattactta cagtatttat tttttccagt
     5161 tgctccggca ttaattatta aagacgatat ccttctttct gtacctattg cgcaatctgc
     5221 aatcaatttt ggggtgattc aaacattaaa aaatttattt cctcatggat ttttggagat
     5281 accaaatatt ctttgttttc aatttttgtc tataaccatg ttttatcaat tattttttaa
     5341 aggctggaaa actttagtac caacttttat gaagcttcga aaagtatatt tagcttcatt
     5401 attagttatt ttaattgctg caatagtaga aggggtattc taatgataga aataaaaagc
     5461 ttgaaaaaaa aatatacaaa taatcaaatt ctcaatgagc tgaattatac tttaaaggat
     5521 gaaatttacg tgctatacgg aaaaaacgga gtaggaaaaa ctacgttaat gaatttatta
     5581 acagggatag aatccccaga ttcaggagaa ataaaaaacg agtcaggatt atcttcctta
     5641 tatttagaag cggtaggaat aggaatgtct gatatgtcaa tcagagataa cattaaattg
     5701 ctttactggt tatatgggga gcgatgcaca gatgaaattc ttgaattgct ttcagaaact
     5761 ttatatagta aagaacagct tgatactgag tatgctttag cttctttagg aatgaaatta
     5821 aaggttggtt tatcactact atatgcagcc aaagagtggg gattgatcgt acttgatgaa
     5881 acattatctg gaattgatcc acaatctaag aaagttatac ttgagcaact aaaaactaaa
     5941 aaatgtccag taattatagt atctcataac tttgataata taaattcata caaaaaaatt
     6001 gagctaacga aagaaggaat agtaaatcaa tgaaagacaa tatatttttt aaagtgatta
     6061 tgtcagtttt ctactcaatt gtattgatgt tgatgttaat agagctaatt acgaaaccga
     6121 tattcaaacc tgtatttgat atagctgttc acgaaacggg aatacctgct agtaaattgc
     6181 taataatttg tggaattgca atctttttgt ttggttttgt tttacttttt tttcaaactg
     6241 gaatttattt tatgggactt aaacttttta acacttcaaa agagaatcta actaaaatct
     6301 ttccttcagt gacgctttcg atgagtattg ttggagtgat atttttattt gtttcaatgt
     6361 ttattgacgt tactaactca ctagttttat tgactacccc agcaatcgtt ttacttttaa
     6421 atagtattat ttattacatt ctttcaaaag atagaaaagg atttttaatc atattggtta
     6481 tctcaatggt aatgtatgct atgaatggtt tagtaaatag aatataaaaa agaggctgtg
     6541 aatattctgc atccttcaaa aaagttaggt tttagtctaa cttttagggg cgttgcattc
     6601 acaatctctt tttttatatt tttatggtta atttttgaga gaaaatttgg ttttcaatgg
     6661 ttgtttctaa atcgtagtta tcgttttctt tagttatttg atttacataa agtagtccta
     6721 gtccatgatt gtttttatcg ctttttgtag tatatcgttc tttctttttg ttcatttcag
     6781 ggaaggttcc ttcaaaagag ttttcaataa taaaattagt gctattgata tctttattga
     6841 tagatatcca aagtgattta tcttcagtag gtatcgtatt ttcaatcgca ttatctaata
     6901 gaatagataa aatacgtact aacatgacta agtcaactgt ttgatcaaaa aatattggct
     6961 gattgattga gagaaaaacg gataggttcc ttctttttgc atactcatat ttataactaa
     7021 ctaagctttt tatttcaata tttttaattt ttgaaagagc tgtattttgt ttacttatat
     7081 ttaaaaggaa cttttctgta ggtgccagca catgctcata agtatcctta atttgcgcca
     7141 gatcctcatt agcaatagct attctaagtg tgtggagtat atttaaataa tcatgccgaa
     7201 acattgctaa atcatcattt aaacgctcaa tctcgttatt gtagtcactt agatttctca
     7261 aaagtaagat attctgttcg ttctctgtgt gatgttttcc aaattggata agtaccaaaa
     7321 gtgtaaataa tccaagtgaa aagaacaagt acattagtcc cttgtcattc aaaaataaat
     7381 cttggataaa ttctattgga gcataacttg ttgtcaattc agaaaacaat acgagcaaga
     7441 atatatttat aagtatcatt gggataagat acttattggg tcgtaaataa tctcgcattg
     7501 aagcgacttt gtctggcttt gctaccctga ttataaatat attaaaagta agaaaaatga
     7561 tgtttaaaac agagactatt agttcttctt ttgaataatt cctaaagatg attgtgttga
     7621 tagattgttc ggtaaaattc aaaaaggccg aaatcaatac agtaaatata cttaaaaata
     7681 tgactaaagt tttcttttcc tgttttttta aataaaaacc aatcagaact aaaattgcaa
     7741 taacaaaaat atactgaaat tgagggaaag aggaggagcc aattacaaaa agaaaaataa
     7801 agatcagtat agctgaaatt gccttatgtt taaatttagt ttgagaactt cctaacagaa
     7861 aaaagtataa agaggtaaca aagtaactgg ataaaaatgc cccaattaag tagaaaataa
     7921 tcattttttt attttaggtt tttctacttc ataaaaccat tgccccataa atttaggtga
     7981 tgtaatagca gtaacatcaa tcgctccctt ttcagctaac acagctgcat ccactttctt
     8041 ttcaattttt tctttgaagt aagattttaa acttttcatt tgtaagcatc tcctgtaata
     8101 aattttttta atggaggata aatcattaac gattgtaata ctaatgtgta aacaagaaga
     8161 ttttgaattt cattaggaca aaagatggaa atgatgaaaa tgcctaaatt tacactgatc
     8221 gctttaactt ttgtattcaa atttttttct tttgtcgttt tcatttctgc agcgttaaac
     8281 tgtggggcat aaatagcagt tagcactaag actacaatcc ctattattgc taaagcaaaa
     8341 taagaaattt ggaaattcat ttttccaata aaatagggaa taactacata aaaaagtgca
     8401 tttaaaacgg agcaacccag tttagatttt gaatgccaac cttttgaata tttgcgcgtt
     8461 actgtcatta aaaagcctac aataatggtt tctttgaaag tcttaaataa agcagccaat
     8521 atgattattt cgcaaagctt ctgtaaccct actaaatagc attcaatata atatcgagtg
     8581 taaattgatc tttcgtcgtt atcatctact gaaataagga aaattttttg tgcgatgtag
     8641 tttaccatta aaacttaatc cctccatata aaaaatataa taacccaaat tttccccatt
     8701 ttaagatgat cgtcctattt gaccattaaa cactcctaac atcacagtat taactgtgtg
     8761 atctctttat aatattttct agaaatagga atttggttcc tacttttggt tttaataagt
     8821 ttatttttac gatcgatatc agtaatatta tttatgttaa caatataaga atgatgtact
     8881 cttttacact ttgatgtaac ggtaatatct cacttaacga accataaaac tccttataga
     8941 aagatatacc gtagatagca attttgtgca ttccgatact ttcaaaatag catataagag
     9001 aaggatcgag tctaaa
//
