BGC0001298 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001298 (Streptomyces calvus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	41,001	High	4-Z-annimycin/4-E-annimycin	PKS:Type I

BGC0001298 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 41,001 nt. (total: 41,001 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 41,002
Label		BGC0001298

Tailoring functions

Ligases: ligase synthase

AGY30674.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AGY30675.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AGY30676.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AGY30677.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AGY30678.1: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001298.4	0.99	PKS	4-Z-annimycin, 4-E-annimycin	Streptomyces calvus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.70	PKS	oronofacic acid	Pseudomonas syringae
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata
BGC0002274.2	0.63	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002277.2	0.62	NRPS	atromentin	Suillus grevillei
BGC0002193.2	0.62	other (other)	phenguignardic acid	Aspergillus terreus NIH2624

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001298.4	0.99	PKS	4-Z-annimycin, 4-E-annimycin	Streptomyces calvus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.70	PKS	oronofacic acid	Pseudomonas syringae
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata
BGC0002274.2	0.63	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002277.2	0.62	NRPS	atromentin	Suillus grevillei
BGC0002193.2	0.62	other (other)	phenguignardic acid	Aspergillus terreus NIH2624

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 41001:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - ccmal - Me-ccmal - ccmal) + (ccmal - ccmal)
Non-colinear ordering:: AGY30676.1 + AGY30677.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AGY30674.1: X

AMP-binding (23..428): X

nrpys: nHcp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GVMTVGGRIK
Nearest Stachelhaus code(s):: nHcp GVMTVGGRIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGY30675.1: X

AMP-binding (10..408): X

nrpys: nLev

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GFCMYGGCSK
Nearest Stachelhaus code(s):: nLev GFCMYGGCSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGY30676.1: mmal - mal - mmal - mal

PKS_AT (534..832): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 224.9
Ethylmalonyl-CoA: 219.3
Methoxymalonyl-CoA: 182.1
2-Methylbutyryl-CoA: 166.0
trans-1,2-CPDA: 126.5
Propionyl-CoA: 124.6
Malonyl-CoA: 107.4
CHC-CoA: 105.6
Isobutyryl-CoA: 86.9
Benzoyl-CoA: 61.0
Acetyl-CoA: 56.5
fatty_acid: 52.3
3-Methylbutyryl-CoA: 45.4
inactive: 32.6

PKS_AT (1519..1809): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.5
inactive: 97.1
Methoxymalonyl-CoA: 67.0
Methylmalonyl-CoA: 61.2
Isobutyryl-CoA: 33.3
Ethylmalonyl-CoA: 31.9
Propionyl-CoA: 28.6
Benzoyl-CoA: 26.2
Acetyl-CoA: 21.6
fatty_acid: 18.1
2-Methylbutyryl-CoA: 15.3
CHC-CoA: 14.8
trans-1,2-CPDA: 10.4
3-Methylbutyryl-CoA: 5.8

PKS_AT (3236..3534): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.6
Ethylmalonyl-CoA: 212.7
Methoxymalonyl-CoA: 183.7
trans-1,2-CPDA: 142.1
2-Methylbutyryl-CoA: 136.1
Propionyl-CoA: 116.7
Malonyl-CoA: 105.4
CHC-CoA: 90.7
Isobutyryl-CoA: 88.4
Benzoyl-CoA: 73.4
3-Methylbutyryl-CoA: 54.8
Acetyl-CoA: 49.3
inactive: 41.1
fatty_acid: 38.5

PKS_AT (4952..5237): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.3
inactive: 106.2
Methoxymalonyl-CoA: 77.4
Methylmalonyl-CoA: 69.0
Propionyl-CoA: 39.1
Ethylmalonyl-CoA: 35.8
Isobutyryl-CoA: 35.6
Acetyl-CoA: 27.3
Benzoyl-CoA: 26.2
2-Methylbutyryl-CoA: 25.7
CHC-CoA: 17.1
trans-1,2-CPDA: 13.9
fatty_acid: 10.5
3-Methylbutyryl-CoA: 0.0

AGY30677.1: mal - mal

PKS_AT (575..875): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.1
inactive: 94.1
Methoxymalonyl-CoA: 78.9
Methylmalonyl-CoA: 66.5
Isobutyryl-CoA: 43.3
Propionyl-CoA: 31.5
Ethylmalonyl-CoA: 25.5
Benzoyl-CoA: 23.4
Acetyl-CoA: 22.9
CHC-CoA: 21.2
fatty_acid: 20.4
trans-1,2-CPDA: 13.9
2-Methylbutyryl-CoA: 4.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (2339..2652): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.9
inactive: 100.1
Methoxymalonyl-CoA: 77.5
Methylmalonyl-CoA: 72.4
Propionyl-CoA: 40.3
Ethylmalonyl-CoA: 32.4
Isobutyryl-CoA: 31.6
fatty_acid: 25.7
Acetyl-CoA: 25.4
CHC-CoA: 18.2
2-Methylbutyryl-CoA: 18.0
Benzoyl-CoA: 14.3
trans-1,2-CPDA: 14.1
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor