BGC0001298 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001298 (Streptomyces calvus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	41,001	4-Z-annimycin/4-E-annimycin	PKS:Type I	100%

BGC0001298 - Region 1 - T1PKS

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Location: 1 - 41,001 nt. (total: 41,001 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 41,002
Label		BGC0001298

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001298	1.00	Polyketide	4-Z-annimycin	Streptomyces calvus
BGC0000041	0.37	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001273	0.32	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001275	0.32	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276	0.32	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244	0.32	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0000343	0.28	NRP	enterobactin	Pseudomonas sp. J465
BGC0000996	0.27	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0001647	0.27	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0000160	0.26	Polyketide	terreic acid	Aspergillus terreus NIH2624

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001298	1.00	Polyketide	4-Z-annimycin	Streptomyces calvus
BGC0000041	0.70	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001273	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001275	0.65	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276	0.65	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244	0.65	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0000343	0.61	NRP	enterobactin	Pseudomonas sp. J465
BGC0000996	0.60	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0001647	0.60	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0000160	0.60	Polyketide	terreic acid	Aspergillus terreus NIH2624

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 41001:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - ccmal - Me-ccmal - ccmal) + (ccmal - ccmal)
Non-colinear ordering:: AGY30676.1 + AGY30677.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AGY30674.1

AMP-binding (23..428): X

nrpys: nHcp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GVMTVGGRIK
Nearest Stachelhaus code(s):: nHcp GVMTVGGRIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGY30675.1

AMP-binding (10..408): X

nrpys: nLev

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GFCMYGGCSK
Nearest Stachelhaus code(s):: nLev GFCMYGGCSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGY30676.1

PKS_AT (534..832): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 224.9
Ethylmalonyl-CoA: 219.3
Methoxymalonyl-CoA: 182.1
2-Methylbutyryl-CoA: 166.0
trans-1,2-CPDA: 126.5
Propionyl-CoA: 124.6
Malonyl-CoA: 107.4
CHC-CoA: 105.6
Isobutyryl-CoA: 86.9
Benzoyl-CoA: 61.0
Acetyl-CoA: 56.5
fatty_acid: 52.3
3-Methylbutyryl-CoA: 45.4
inactive: 32.6

PKS_AT (1519..1809): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.5
inactive: 97.1
Methoxymalonyl-CoA: 67.0
Methylmalonyl-CoA: 61.2
Isobutyryl-CoA: 33.3
Ethylmalonyl-CoA: 31.9
Propionyl-CoA: 28.6
Benzoyl-CoA: 26.2
Acetyl-CoA: 21.6
fatty_acid: 18.1
2-Methylbutyryl-CoA: 15.3
CHC-CoA: 14.8
trans-1,2-CPDA: 10.4
3-Methylbutyryl-CoA: 5.8

PKS_AT (3236..3534): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.6
Ethylmalonyl-CoA: 212.7
Methoxymalonyl-CoA: 183.7
trans-1,2-CPDA: 142.1
2-Methylbutyryl-CoA: 136.1
Propionyl-CoA: 116.7
Malonyl-CoA: 105.4
CHC-CoA: 90.7
Isobutyryl-CoA: 88.4
Benzoyl-CoA: 73.4
3-Methylbutyryl-CoA: 54.8
Acetyl-CoA: 49.3
inactive: 41.1
fatty_acid: 38.5

PKS_AT (4952..5237): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.3
inactive: 106.2
Methoxymalonyl-CoA: 77.4
Methylmalonyl-CoA: 69.0
Propionyl-CoA: 39.1
Ethylmalonyl-CoA: 35.8
Isobutyryl-CoA: 35.6
Acetyl-CoA: 27.3
Benzoyl-CoA: 26.2
2-Methylbutyryl-CoA: 25.7
CHC-CoA: 17.1
trans-1,2-CPDA: 13.9
fatty_acid: 10.5
3-Methylbutyryl-CoA: 0.0

AGY30677.1

PKS_AT (575..875): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.1
inactive: 94.1
Methoxymalonyl-CoA: 78.9
Methylmalonyl-CoA: 66.5
Isobutyryl-CoA: 43.3
Propionyl-CoA: 31.5
Ethylmalonyl-CoA: 25.5
Benzoyl-CoA: 23.4
Acetyl-CoA: 22.9
CHC-CoA: 21.2
fatty_acid: 20.4
trans-1,2-CPDA: 13.9
2-Methylbutyryl-CoA: 4.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (2339..2652): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.9
inactive: 100.1
Methoxymalonyl-CoA: 77.5
Methylmalonyl-CoA: 72.4
Propionyl-CoA: 40.3
Ethylmalonyl-CoA: 32.4
Isobutyryl-CoA: 31.6
fatty_acid: 25.7
Acetyl-CoA: 25.4
CHC-CoA: 18.2
2-Methylbutyryl-CoA: 18.0
Benzoyl-CoA: 14.3
trans-1,2-CPDA: 14.1
3-Methylbutyryl-CoA: 0.0