LOCUS       BGC0001307              4083 bp    DNA     linear   BCT 21-DEC-2015
DEFINITION  Streptomyces leeuwenhoekii strain C58 chaxapeptin biosynthesis gene
            cluster, partial sequence.
ACCESSION   BGC0001307
VERSION     BGC0001307
KEYWORDS    .
SOURCE      Streptomyces leeuwenhoekii
  ORGANISM  Streptomyces leeuwenhoekii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 15:18:22
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4083
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             <1..399
                     /codon_start=1
                     /note="orf-1"
                     /product="putative membrane protein"
                     /protein_id="ALT06551.1"
                     /transl_table=11
                     /translation="MGVIGGLGWLVDTLTFNFFLNILGLASVRSGLLASTIAIAFNYLG
                     HRYWTYRSSATVSRSREVGLFALFSVIGMAIQNGVLAISHYGFGYTSAWADNIAKNMVG
                     LGAATVFRFWAYRNLVFHVTHQSPADAR"
     source          1..4083
                     /db_xref="taxon:1437453"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces leeuwenhoekii"
                     /strain="C58"
     misc_feature    <1..>4083
                     /note="chaxapeptin biosynthesis gene cluster"
     subregion       1..4083
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4083
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            630..764
                     /gene="cptA"
     CDS             630..764
                     /codon_start=1
                     /gene="cptA"
                     /product="precursor peptide"
                     /protein_id="ALT06552.1"
                     /transl_table=11
                     /translation="MEPQMTELQPEAYEAPSLIEVGEFSEDTLGFGSKPLDSFGLNFF"
     gene            772..2586
                     /gene="cptC"
     CDS             772..2586
                     /codon_start=1
                     /gene="cptC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 113.2; E-value: 2.2e-34)"
                     /gene_kind="biosynthetic"
                     /product="lasso peptide cyclase"
                     /protein_id="ALT06553.1"
                     /sec_met_domain="Asn_synthase (E-value: 1.2e-39, bitscore:
                     125.8, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTGFLILPDSVGTDALRDRLPLLALRTLRYPSGRPWIVGRWDAE
                     EIMSARVGPVHVVVIGFCPVTTTELTSLISRIHTVSDVNRLVASLPGSFHLVAAVGRQL
                     RVQGSITGLRRIAWAKSDGHTFVSDRTDILADLTNAKIDDSLLATRVACGGMVPPPLRE
                     RSMWLGVNSLPPDRYLLVEGGQRTREVKWWQPPAPETTLAEGACRVREALVSALYSRNP
                     VHGRLSADLSGGLDSTSLAFLAARTTPHLLTHRWAEAEAGNDDGKYAAIAARELPNAEH
                     LVIPQSDIPPVFSDPHLNGDPEQPYLFARTAARAVYTARMLVAHSSTRHIAGHGGDELF
                     YKFPGYLHPLLRRRPLTAIRHIRAHLALSRWDRKATVAQLLDTSGFPDWWKRQADQLTA
                     PPPGGRFPPLGWGFMPLHAQPWVTGEALHAARATLREAAEEAHPLAPDRGQHQYLLALR
                     TTAPAYAQLGRLFERAGVRLDLPYLDDRVVEAALSVRLEERAHPWRYKPLLAEAMRGVV
                     PESIATRVTKGEFSADLSLGLRRNRASVLDFLADSAIAKRGLIDVDTLRRLLLAPQADH
                     SRNIALEHLVGCETWLRTTPKRQDAPAP"
     gene            2570..2827
                     /gene="cptB1"
     CDS             2570..2827
                     /codon_start=1
                     /gene="cptB1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic-additional"
                     /product="hypothetical protein"
                     /protein_id="ALT06554.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 2.7e-27,
                     bitscore: 83.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 5.9e-18, bitscore: 54.0,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLHPDIVIAETEDGAVLLHQRTGRYWQLNTTGMAVIESLTSGET
                     LAEITHRMAKRYGLPAEQVRQDVEAVVHQLHAAQIVESTS"
     gene            2824..3240
                     /gene="cptB2"
     CDS             2824..3240
                     /codon_start=1
                     /gene="cptB2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /product="hypothetical protein"
                     /protein_id="ALT06555.1"
                     /sec_met_domain="PF13471 (E-value: 3e-30, bitscore: 93.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTPAALVKRRKLPFHRRVLPLVAVGVARLLSRTKPARLRAILSY
                     ARRGAAPATVEQAMAARQAVVAVSQRCAGQACLQRSIATALLCRARGSWPTWCTGVRTS
                     PFEAHAWIEAEGRLIGEPYPDGHYRPLLTVPPVS"
     CDS             complement(3448..3831)
                     /codon_start=1
                     /note="orf1"
                     /product="hypothetical protein"
                     /protein_id="ALT06556.1"
                     /transl_table=11
                     /translation="MRAAAVAALALTPLTAPASASSEPHAARAAIDCPSGYVCIYPEIN
                     FGGQPWVRRASDGSVKDLPSAIRDRGSSIRNNSDRTARIYEKRNHAGRWVCVTRSGGSI
                     HDLRGYNLNDQTRSLKINRNDCG"
     CDS             3921..>4083
                     /codon_start=1
                     /note="orf2"
                     /product="hypothetical protein"
                     /protein_id="ALT06557.1"
                     /transl_table=11
                     /translation="MASAMPGSTRCSGTTARCAANVRRQPPEYGRPVGAQYRGYGYVPV
                     PCLSGGSLM"
ORIGIN
        1 ttcggcgtga ttggcggcct agggtggctt gtcgacacgc tgacattcaa cttcttcctg
       61 aatattttgg gactagcctc tgtccggtcg ggacttctgg cgagcaccat cgccatagcg
      121 ttcaactatt tagggcatcg gtactggacc tatcggtcgt ccgccacggt aagccgttca
      181 cgcgaggtcg gacttttcgc cctctttagt gtcattggca tggcgatcca gaacggcgtg
      241 ctcgctattt ctcattacgg attcggatat acttcagctt gggctgacaa tatcgcgaag
      301 aacatggttg ggctgggagc ggccaccgtc ttccggttct gggcgtaccg caacttagta
      361 tttcacgtta cgcatcaatc accggccgac gcgcgatgac gtgaccagca taggaggcga
      421 accttcgttg cgccgcactt catgatcagc cagtggctcg cggcgaaatc taccttttgg
      481 tgccggtccg ccgacgtccg accctcactg aacagcgaag ctcggcccga ctccgagcaa
      541 gatcgctctg gctggaaacc ttctggatcg agcgcgtgag cgaatgtagg tttcgtgttg
      601 atcgcaaccc ctgagcatgg aggatcgtca tggaacccca gatgactgag cttcagccgg
      661 aggcctacga ggcgccgtcc ctcatcgagg tgggcgagtt ctcggaggac acgctcggct
      721 tcggaagcaa gccgctggac agcttcggcc tgaacttctt ttaggccaac agtgagcacc
      781 gggtttctta tcctgccgga ctctgtcggt actgatgccc tccgcgacag gcttccgttg
      841 ctggcgttac gcacgcttcg gtacccatcg gggcggccgt ggatcgtcgg cagatgggac
      901 gctgaggaga tcatgtccgc gcgtgtcggc ccggtgcacg tggtggtgat cgggttttgc
      961 ccggtcacca ccaccgaact gacctcgttg atcagtcgga tccacaccgt ctctgacgtg
     1021 aatcgtttgg ttgccagtct gcctggaagc ttccacctcg tcgccgcggt cgggcggcag
     1081 ttacgggttc agggcagcat cacgggtcta cgcagaatcg cttgggcaaa gagcgacggc
     1141 cataccttcg tctccgaccg caccgacata ctcgccgacc tgacgaacgc aaaaatcgac
     1201 gattctctcc tcgccacgag agtggcatgc ggcgggatgg tccccccacc gctgcgtgaa
     1261 cgcagtatgt ggcttggcgt caactctctg ccgcccgacc gctatctctt ggtcgagggc
     1321 gggcagcgga cgcgcgaggt gaaatggtgg cagccgccag caccagaaac gactcttgct
     1381 gagggcgcct gcagagtcag ggaagctctc gtatcggccc tgtacagcag gaaccccgtc
     1441 cacggccgac tcagcgcgga cctgtcaggc gggcttgact ctacctcgct ggcctttctc
     1501 gccgcccgga ccacgcctca tctactcaca caccgctggg cggaggccga ggcgggcaat
     1561 gacgacggca agtatgcggc gatcgcggcg cgggagttgc ccaatgctga acaccttgtc
     1621 attccgcaat cagatatacc gcctgtcttc tccgacccgc acctgaacgg cgaccccgag
     1681 caaccctatc ttttcgcgcg gacggctgcc cgagccgtgt atacggctcg tatgctagtc
     1741 gcgcatagtt ccacgagaca tatagccggc catgggggag acgaactctt ctacaagttc
     1801 cctggatatc ttcaccctct gctgcgtcgg cgtccactca ccgccatccg gcatattcgc
     1861 gcccatctgg cgctcagccg gtgggacagg aaagccaccg tcgcacaatt gctggacacc
     1921 agtggtttcc cggattggtg gaagcggcag gcggaccaac ttactgcacc tccaccaggc
     1981 ggacgttttc cgcctttggg ctggggattt atgcctctgc acgcccagcc ctgggtgaca
     2041 ggggaggcgc tgcacgcggc acgcgccaca ctccgcgagg cggcggaaga ggcccacccc
     2101 ctcgccccgg accgggggca acaccagtac ctgctggccc tgcgtaccac cgcgccggca
     2161 tacgcacagc tcggtcgcct attcgagcgg gcgggtgtcc ggctggactt gccatacctg
     2221 gatgaccggg tcgtagaagc ggccctgtct gtccggctgg aggagcgggc ccacccctgg
     2281 cgttacaaac cacttctcgc ggaggccatg cggggtgtcg tcccggagag catcgctacc
     2341 cgcgtcacca agggagagtt cagtgccgat ttgagtctcg ggttacgccg gaaccgcgcg
     2401 agcgtgctgg actttctcgc cgattctgcc atcgccaaga gggggctgat cgacgtcgac
     2461 accctgcgtc gcctgcttct cgcgccccag gctgatcact cccgaaatat agctctcgaa
     2521 cacctcgtgg gctgtgagac ctggctccgc accaccccaa agagacaaga tgcgcctgca
     2581 ccctgatatc gtcatcgcgg aaaccgaaga cggtgcggtc ctactacacc aaaggaccgg
     2641 ccgctactgg cagctgaaca ccacgggcat ggcggtcatc gaaagcctga cttccggtga
     2701 gaccctcgcc gagatcacgc accgtatggc caagcgttac ggcttgcccg ctgagcaggt
     2761 ccggcaggac gtcgaagccg tcgtccatca actacacgcg gcccagatag tggagagcac
     2821 ctcgtgagta cccccgccgc cctggtcaag cgcagaaaac tgcccttcca tcgcagagtg
     2881 ctgccgctcg ttgctgtcgg tgttgcccgc ctcctcagca ggacaaagcc tgcacggctg
     2941 cgagccatcc tgagctacgc acgacggggt gccgcgccgg ccacggtgga acaggcgatg
     3001 gccgcgcgac aagcggtcgt cgccgtcagc caacggtgcg ccggacaggc ctgccttcag
     3061 cgttccatcg cgacggcact gctctgccgg gcgcgtggat cctggccgac ctggtgcacc
     3121 ggggtacgga ccagcccgtt cgaagcccat gcctggatcg aggccgaagg tcggctcatc
     3181 ggcgagccgt acccggacgg tcactatcgg cctttgctga ccgttcctcc ggtttcctga
     3241 caccggtcga cacgaccgac gacggtcggc tgccgactca ccgtgcacct ccccggacca
     3301 cgcccgccgc ctgatcgtgg ccagccaatg cgagagccag cgaccagacg gcccggccgc
     3361 ccccaacgcg gagacgcccg cccccgtgga ggcccccggg ccgggactgg ccggacgcac
     3421 cgatcccggc atgcggcggg accgggttca gccgcagtcg ttccggttga tcttcaggga
     3481 gcgggtctga tcgttgaggt tgtagccgcg caggtcgtgg atggatccgc cgctgcgggt
     3541 gacgcacacc cagcggccgg cgtggttgcg cttctcgtag atgcgggcgg tgcggtcgga
     3601 gttgttgcgg atggagctgc cgcggtcccg gatcgcggaa ggcaggtcct tcacgctgcc
     3661 atcggaagcc cggcgtaccc agggctggcc gccgaagttg atctcggggt agatgcagac
     3721 gtagccggac gggcagtcga tcgcggcccg tgccgcatgg ggctcgctgg aagccgaggc
     3781 cggagcggtc agcggggtga gggcgagggc ggcgacggcc gcagcacgca tcaggttcct
     3841 aatcacgtac tacggatact gctcgccgcc cgtgagtagc gtcgcttgcg gccgcgttca
     3901 ctccagcgga gcagttctcc atggcctccg cgatgcccgg gtccacccgc tgctcgggga
     3961 cgacggccag gtgtgcggcc aatgtgcggc ggcagccgcc cgaatatggc agaccagtcg
     4021 gggcccagta ccgtgggtac gggtacgtac ccgtaccctg cttgtctgga gggagcctga
     4081 tga
//
