BGC0001327: rakicidin A biosynthetic gene cluster from Micromonospora purpureochromogenes
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Location: 164,252 - 202,669 nt. (total: 38,418 nt).
This entry is originally from NCBI GenBank NZ_JOEU01000001.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001327
Short description rakicidin A biosynthetic gene cluster from Micromonospora purpureochromogenes
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide (Macrolide)
Loci NCBI GenBank: NZ_JOEU01000001.1
Compounds
  • rakicidin A
  • rakicidin B
Species Micromonospora purpureochromogenes [taxonomy]
References
Chemical products information
rakicidin A
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C32H54N4O7
Chemical database entries
NPAtlas
rakicidin B
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C33H56N4O7
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • IH31_RS0100575
  • WP_036342114.1
 164252 - 172972 (+) type I polyketide synthase
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  • IH31_RS34685
  • WP_052165465.1
 172969 - 177789 (+) type I polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • IH31_RS0100585
  • WP_030498974.1
 177791 - 181162 (+) tyrocidine synthase 3
  • Scaffold biosynthesis
Sequence-based prediction
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  • IH31_RS0100590
  • WP_030498975.1
 181179 - 186842 (+) type I polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • IH31_RS0100595
  • WP_052165466.1
 186946 - 191502 (+) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • IH31_RS0100600
  • WP_030498977.1
 191499 - 196208 (+) hypothetical protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • IH31_RS0100605
  • WP_030498978.1
 196205 - 197917 (+) hypothetical protein
  • Transport
Sequence-based prediction
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  • IH31_RS0100610
  • WP_084212913.1
 197871 - 198734 (+) alpha/beta fold hydrolase
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  • IH31_RS0100615
  • WP_030498980.1
 198731 - 201598 (+) hypothetical protein
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  • IH31_RS0100620
  • WP_030498981.1
 201680 - 202669 (+) clavaminate synthase
  • Tailoring (Monooxygenation)
Sequence-based prediction
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Polyketide-specific information
Subclass Macrolide
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
IH31_RS0100590
+
IH31_RS34685 		Synthase subclass: Modular type I
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: IH31_RS34685
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 3
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: IH31_RS0100590
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
Thioesterase genes
  • IH31_RS0100610 (Unknown)
NRP-synthases
Gene Modules
IH31_RS0100585
Module 2
Specificity: serine
Evidence for specificity:
  • Feeding study
Condensation domain type: LCL
IH31_RS0100595
Module 4
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Modification domains: Methylation
IH31_RS0100600
Module 5
Specificity: 3S-hydroxyasparagine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 6 [inactive]
Condensation domain type: LCL
Non-canonical activity:
  • Other
Evidence for non-canonical activity:
  • Structure-based inference
IH31_RS0100615
Module 7 [inactive]
Condensation domain type: DCL
Non-canonical activity:
  • Other
Evidence for non-canonical activity:
  • Structure-based inference
Annotation changelog
MIBiG version Submitter Notes
1.4
  • Hidden contributor (ID: DPAKSDGGIIU3JRGIDNMS7RSG, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated reference publication(s) (MIBiG Annotathon)
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed gene names of 'No gene ID'
  • Removed gene annotation with no reference name or ID
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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