Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001328.1:
Cluster 1NRP / Polyketide138897

BGC0001328, rakicidin A / rakicidin B biosynthetic gene cluster from Micromonospora sp. M42. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Rakicidin A
SMILES string: Copy to clipboard
Molecular formula: C32H54N3O7
Exact molecular mass: 607.4065 Da ([M+H]+)
Molecular activity: Cytotoxic

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Cyclic depsipeptide (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
No gene ID (EWM63002.1)
Module 3
A specificity: Serine
Evidence for specificity: Feeding study
C domain subtype: LCL
No gene ID (EWM63005.1)
Module 5
A specificity: Glycine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
No gene ID (EWM63006.1)
Module 6
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
No gene ID (EWM63007.1)
Module 6
A specificity: Beta-hydroxyasparagine
Evidence for specificity: Structure-based inference
C domain subtype: N/A
Module 7
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
Module is skipped
Evidence for skipping: Unknown
No gene ID (EWM63010.1)
Module 8
A specificity: None
Evidence for specificity: None
C domain subtype: DCL
Module is skipped
Evidence for skipping: Unknown
Polyketide subclass:
Macrolide (cyclic)
Polyketide synthase subclass:
Modular type I
Starter unit:
Other
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactonization

Modular polyketide synthases:
No gene ID (EWM62997.1)
Module 0
AT specificity: None
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None
Module 1
Scaffold-modifying domain: None

No gene ID (EWM63000.1)
Module 2
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None

No gene ID (EWM63003.1)
Module 4
Scaffold-modifying domain: None

Gene cluster description

rakicidin A / rakicidin B (BGC0001328). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank KK037233, positions 194525-233421. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Thomas Torring (Aarhus University)

Literature references

1. Tsakos M et al. (2016) Total Synthesis and Biological Evaluation of Rakicidin A and Discovery of a Simplified Bioactive Analogue. Angew Chem Int Ed Engl 55(3):1030-5. doi: 10.1002/anie.201509926.