Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001330.1:
Cluster 1NRP / Polyketide143587

BGC0001330, BE-43547 A1-C2 biosynthetic gene cluster from Micromonospora sp. RV43. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: BE-43547A1
PubChem ID: 9959574
SMILES string: Copy to clipboard
Molecular formula: C30H49N3O7
Average molecular mass: 563.736 Da
Molecular activity: Cytotoxic

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Cyclic depsipeptide (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
WP_047890612.1
Module 0
A specificity: None
Evidence for specificity: Structure-based inference
C domain subtype: N/A
Module is skipped
Evidence for skipping: Unknown
WP_053065269.1
Module 3
A specificity: Glycine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 4
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
WP_053065270.1
Module 6
A specificity: Glycine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Methylation
Module 7
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
Module is skipped
Evidence for skipping: Unknown
Polyketide subclass:
Macrolide (cyclic)
Polyketide synthase subclass:
Modular type I
Starter unit:
Other
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactonization

Modular polyketide synthases:
WP_053065267.1
Module 0
AT specificity: None
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None
Module 1
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: L-OH
Scaffold-modifying domain: None

WP_053065268.1
Module 2
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None

WP_047890614.1
Module 5
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

Gene cluster description

BE-43547 A1-C2 (BGC0001330). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank NZ_LEKG01000004, positions 20144-63730. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Thomas Torring (Aarhus University)

Literature references

1. Michail Tsakos, Lise L. Clement, Eva S. Schaffert, Frank N. Olsen, Sebastiano Rupiani, Rasmus Djurhuus, Wanwan Yu, Kristian M. Jacobsen, Nikolaj L. Villadsen, Thomas B. Poulsen. (2015) Total Synthesis and Biological Evaluation of Rakicidin A and Discovery of a Simplified Bioactive Analogue. Angewandte Chemie International Edition, vol. 55, no. 3, pp. 1030-1035