BGC0001330: BE-43547A1 biosynthetic gene cluster from Micromonospora sp. RV43
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Location: 20,144 - 63,730 nt. (total: 43,587 nt).
This entry is originally from NCBI GenBank NZ_LEKG01000004.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001330
Short description BE-43547A1 biosynthetic gene cluster from Micromonospora sp. RV43
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide (Macrolide)
Loci NCBI GenBank: NZ_LEKG01000004.1
Compounds
  • BE-43547A1
  • BE-43547A2
  • BE-43547B1
  • BE-43547B2
  • BE-43547B3
  • BE-43547C1
  • BE-43547C2
Species Micromonospora sp. RV43 [taxonomy]
References
Chemical products information
BE-43547A1
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C30H49N3O7
Chemical database entries
PubCHEM
BE-43547A2
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C30H49N3O7
BE-43547B1
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C31H51N3O7
BE-43547B2
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C31H51N3O7
BE-43547B3
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C31H51N3O7
BE-43547C1
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C32H53N3O7
BE-43547C2
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C32H53N3O7
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ABD52_RS02340
  • WP_047890608.1
 20144 - 21163 (-) NAD(P)-binding domain-containing protein
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  • ABD52_RS02345
  • WP_200905152.1
 21765 - 22760 (+) polyprenyl synthetase family protein
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  • ABD52_RS02350
  • WP_161801596.1
 22966 - 23646 (+) hypothetical protein
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  • ABD52_RS02355
  • WP_053065262.1
 23681 - 24787 (+) hypothetical protein
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  • ABD52_RS02360
  • WP_082171176.1
 24777 - 25730 (+) UbiA family prenyltransferase
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  • ABD52_RS02365
  • WP_047890612.1
 25777 - 27393 (+) acyl--CoA ligase
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  • ABD52_RS02370
  • WP_053065263.1
 27435 - 28205 (+) hypothetical protein
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  • ABD52_RS02375
  • WP_053065264.1
 28270 - 30507 (+) MMPL family transporter
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  • ABD52_RS02380
  • WP_152748170.1
 30624 - 30869 (-) hypothetical protein
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  • ABD52_RS37315
  • WP_053065266.1
 31472 - 31960 (+) hypothetical protein
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  • ABD52_RS02390
  • WP_047890613.1
 31957 - 32433 (+) flavodoxin/nitric oxide synthase
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  • ABD52_RS02395
  • WP_053065267.1
 32475 - 39212 (+) type I polyketide synthase
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  • ABD52_RS02400
  • WP_053065268.1
 39214 - 42207 (+) type I polyketide synthase
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  • ABD52_RS02405
  • WP_053065269.1
 42204 - 48635 (+) non-ribosomal peptide synthetase
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  • ABD52_RS02410
  • WP_047890614.1
 48638 - 54103 (+) type I polyketide synthase
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  • ABD52_RS02415
  • WP_053065270.1
 54100 - 59772 (+) non-ribosomal peptide synthetase
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  • ABD52_RS02420
  • WP_047890615.1
 59769 - 60518 (+) alpha/beta fold hydrolase
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  • ABD52_RS02425
  • WP_047890616.1
 60570 - 61157 (-) TetR/AcrR family transcriptional regulator
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  • ABD52_RS02430
  • WP_047890617.1
 61248 - 62174 (+) ABC transporter ATP-binding protein
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  • ABD52_RS02435
  • WP_047890618.1
 62171 - 63730 (+) hypothetical protein
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Polyketide-specific information
Subclass Macrolide
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
ABD52_RS02395
+
ABD52_RS02400
+
ABD52_RS02410 		Synthase subclass: Modular type I
Module 0
Genes: ABD52_RS02395
Core domains: CoA-ligase, Thiolation (ACP/PCP)
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: ABD52_RS02395
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 2
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: ABD52_RS02400
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
Module 5
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: ABD52_RS02410
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
Thioesterase genes
  • ABD52_RS02420 (Unknown)
NRP-synthases
Gene Modules
ABD52_RS02365
Module 0 [inactive]
Non-canonical activity:
  • Other
Evidence for non-canonical activity:
  • Structure-based inference
ABD52_RS02405
Module 3
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 4
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
ABD52_RS02415
Module 6
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Modification domains: Methylation
Module 7 [inactive]
Condensation domain type: LCL
Non-canonical activity:
  • Other
Evidence for non-canonical activity:
  • Structure-based inference
Annotation changelog
MIBiG version Submitter Notes
1.4
  • Hidden contributor (ID: DPAKSDGGIIU3JRGIDNMS7RSG, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated reference publication(s) (MIBiG Annotathon)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Changed amino acid substrates to lower case
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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