BGC0001332 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001332 (Streptomyces sp. MspMP-M5)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	62,086	High	microtermolide A	NRPS:Type I+PKS

BGC0001332 - Region 1 - NRPS,T1PKS

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Location: 1 - 62,086 nt. (total: 62,086 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 62,087
Label		BGC0001332

Tailoring functions

Oxidoreductases: dehydrogenase

B073_RS0123840: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

B073_RS0123870: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

B073_RS0123875: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

B073_RS0123895: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acylase aminotransferase

B073_RS0123845: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

B073_RS0123885: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

B073_RS0123855: thioesterase

smcogs: SMCOG1004: thioesterase

B073_RS0123860: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

B073_RS0123880: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

B073_RS0123900: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

B073_RS40860: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001332.5	1.71	NRPS, PKS	microtermolide A	Streptomyces sp. MspMP-M5
BGC0001050.5	0.83	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0001273.3	0.81	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.80	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002135.2	0.80	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001244.3	0.80	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001833.4	0.77	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.76	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001332.5	0.98	NRPS, PKS	microtermolide A	Streptomyces sp. MspMP-M5
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001050.5	0.73	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 62086:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - D-Val) + (mal) + (Ser) + (ccmal) + (Me-mal - ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

B073_RS40860: mmal - mal

PKS_AT (559..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.9
Ethylmalonyl-CoA: 194.0
Methoxymalonyl-CoA: 165.7
2-Methylbutyryl-CoA: 126.5
trans-1,2-CPDA: 114.9
Propionyl-CoA: 106.8
Malonyl-CoA: 94.3
CHC-CoA: 81.5
Isobutyryl-CoA: 75.1
Benzoyl-CoA: 55.8
Acetyl-CoA: 32.3
3-Methylbutyryl-CoA: 21.7
inactive: 21.4
fatty_acid: 18.8

PKS_AT (1574..1868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.8
inactive: 99.9
Methoxymalonyl-CoA: 76.0
Methylmalonyl-CoA: 74.8
Propionyl-CoA: 39.4
Isobutyryl-CoA: 33.5
Ethylmalonyl-CoA: 33.0
Benzoyl-CoA: 21.1
fatty_acid: 20.9
2-Methylbutyryl-CoA: 19.7
3-Methylbutyryl-CoA: 18.0
Acetyl-CoA: 17.8
CHC-CoA: 14.7
trans-1,2-CPDA: 12.9

B073_RS0123875: mal

PKS_AT (550..844): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 166.7
inactive: 96.1
Methoxymalonyl-CoA: 77.9
Methylmalonyl-CoA: 76.8
Propionyl-CoA: 65.0
Ethylmalonyl-CoA: 46.2
CHC-CoA: 40.4
2-Methylbutyryl-CoA: 38.3
Isobutyryl-CoA: 37.4
Benzoyl-CoA: 35.6
trans-1,2-CPDA: 28.3
Acetyl-CoA: 28.3
fatty_acid: 21.4
3-Methylbutyryl-CoA: 11.3

B073_RS0123880: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (494..890): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

B073_RS0123885: mal

PKS_AT (556..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 161.3
inactive: 109.6
Methoxymalonyl-CoA: 82.4
Methylmalonyl-CoA: 69.8
Propionyl-CoA: 45.3
Ethylmalonyl-CoA: 39.4
CHC-CoA: 38.3
2-Methylbutyryl-CoA: 35.2
Isobutyryl-CoA: 34.2
fatty_acid: 23.4
Benzoyl-CoA: 23.2
trans-1,2-CPDA: 21.3
Acetyl-CoA: 20.8
3-Methylbutyryl-CoA: 4.8

B073_RS0123900: Ala - Val

Search NORINE for peptide: strict or relaxed

AMP-binding (501..893): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLCMIYK
Nearest Stachelhaus code(s):: Ala DILQLCMIYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1559..1959): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYFLGGTFK
Nearest Stachelhaus code(s):: Val DAYFLGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor