BGC0001342 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001342 (Chondromyces crocatus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	81,745	High	Cmc-thuggacin A/Cmc-thuggacin B	NRPS:Type I+PKS

BGC0001342 - Region 1 - NRPS,T1PKS

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Location: 1 - 81,745 nt. (total: 81,745 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 81,746
Label		BGC0001342

Tailoring functions

Transferases: acylase

tugD: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: synthase

tugA: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

tugB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tugC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001342.3	1.71	NRPS, PKS	Cmc-thuggacin A, Cmc-thuggacin B	Chondromyces crocatus
BGC0001276.3	0.90	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001051.5	0.78	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0001275.3	0.65	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.65	PKS	asperlactone	Aspergillus ochraceus
BGC0000041.3	0.65	PKS	oronofacic acid	Pseudomonas syringae
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000067.5	0.55	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001342.3	1.00	NRPS, PKS	Cmc-thuggacin A, Cmc-thuggacin B	Chondromyces crocatus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001051.5	0.72	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0000041.3	0.71	PKS	oronofacic acid	Pseudomonas syringae
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001164.3	0.65	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001244.3	0.65	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.65	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000067.5	0.62	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 81745:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-redmal) + (ohmal - ohmal) + (ohmal - ccmal) + (ohmal - Me-ohmal - Cys - Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

tugA: mmal - pk - pk - mmal

PKS_AT (630..926): mmal

ATSignature: Methylmalonyl-CoA, Benzoyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 66.7%; Benzoyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Benzoyl-CoA

Prediction, score:

Benzoyl-CoA: 163.7
Methylmalonyl-CoA: 127.5
Isobutyryl-CoA: 123.9
Methoxymalonyl-CoA: 104.4
Ethylmalonyl-CoA: 100.2
Malonyl-CoA: 81.8
Propionyl-CoA: 74.9
2-Methylbutyryl-CoA: 71.9
trans-1,2-CPDA: 69.6
CHC-CoA: 55.5
Acetyl-CoA: 53.0
3-Methylbutyryl-CoA: 41.2
inactive: 32.0
fatty_acid: 30.5

PKS_AT (1073..1370): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 207.3
Methylmalonyl-CoA: 206.8
Methoxymalonyl-CoA: 173.6
2-Methylbutyryl-CoA: 141.9
trans-1,2-CPDA: 138.2
CHC-CoA: 111.3
Malonyl-CoA: 107.7
Propionyl-CoA: 105.3
Isobutyryl-CoA: 83.7
Benzoyl-CoA: 77.3
Acetyl-CoA: 48.7
fatty_acid: 43.8
inactive: 33.4
3-Methylbutyryl-CoA: 24.3

PKS_AT (2853..3150): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 207.3
Methylmalonyl-CoA: 206.8
Methoxymalonyl-CoA: 173.6
2-Methylbutyryl-CoA: 141.9
trans-1,2-CPDA: 138.2
CHC-CoA: 111.3
Malonyl-CoA: 107.7
Propionyl-CoA: 105.3
Isobutyryl-CoA: 83.7
Benzoyl-CoA: 77.3
Acetyl-CoA: 48.7
fatty_acid: 43.8
inactive: 33.4
3-Methylbutyryl-CoA: 24.3

PKS_AT (4623..4920): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.7
Ethylmalonyl-CoA: 206.7
Methoxymalonyl-CoA: 176.7
2-Methylbutyryl-CoA: 143.6
trans-1,2-CPDA: 137.5
CHC-CoA: 111.9
Malonyl-CoA: 111.5
Propionyl-CoA: 103.9
Isobutyryl-CoA: 87.1
Benzoyl-CoA: 82.6
Acetyl-CoA: 51.3
fatty_acid: 43.5
inactive: 36.3
3-Methylbutyryl-CoA: 24.5

tugB: mal - mal

PKS_AT (550..849): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.3
inactive: 127.3
Methoxymalonyl-CoA: 71.2
Methylmalonyl-CoA: 50.4
Propionyl-CoA: 32.9
Isobutyryl-CoA: 29.5
Benzoyl-CoA: 18.5
Acetyl-CoA: 17.4
Ethylmalonyl-CoA: 11.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (2079..2380): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.1
inactive: 107.2
Methoxymalonyl-CoA: 55.8
Methylmalonyl-CoA: 54.4
Propionyl-CoA: 30.6
Isobutyryl-CoA: 27.3
Acetyl-CoA: 20.1
2-Methylbutyryl-CoA: 19.1
Benzoyl-CoA: 14.4
Ethylmalonyl-CoA: 13.8
fatty_acid: 8.4
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

tugC: mal - mal

PKS_AT (587..888): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.1
inactive: 101.6
Methoxymalonyl-CoA: 57.0
Methylmalonyl-CoA: 53.2
Propionyl-CoA: 31.3
Isobutyryl-CoA: 28.0
2-Methylbutyryl-CoA: 19.9
Acetyl-CoA: 17.3
Ethylmalonyl-CoA: 15.2
Benzoyl-CoA: 14.4
fatty_acid: 8.1
CHC-CoA: 1.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2156..2457): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.9
inactive: 107.3
Methoxymalonyl-CoA: 56.6
Methylmalonyl-CoA: 54.0
Propionyl-CoA: 31.0
Isobutyryl-CoA: 28.2
2-Methylbutyryl-CoA: 19.9
Acetyl-CoA: 18.2
Ethylmalonyl-CoA: 15.2
Benzoyl-CoA: 14.6
fatty_acid: 9.3
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

tugD: mal - mmal - Cys - mmal

Search NORINE for peptide: strict or relaxed

PKS_AT (561..862): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.9
inactive: 112.6
Methoxymalonyl-CoA: 63.2
Methylmalonyl-CoA: 49.6
Propionyl-CoA: 30.4
Isobutyryl-CoA: 29.6
Acetyl-CoA: 20.7
Benzoyl-CoA: 19.9
2-Methylbutyryl-CoA: 10.7
Ethylmalonyl-CoA: 6.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2123..2420): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.0
Ethylmalonyl-CoA: 192.2
Methoxymalonyl-CoA: 174.6
2-Methylbutyryl-CoA: 130.3
trans-1,2-CPDA: 126.7
CHC-CoA: 101.4
Isobutyryl-CoA: 94.3
Malonyl-CoA: 92.0
Propionyl-CoA: 91.9
Benzoyl-CoA: 70.7
3-Methylbutyryl-CoA: 53.1
Acetyl-CoA: 44.6
fatty_acid: 40.6
inactive: 26.6

AMP-binding (3596..3995): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLWNLSLIWK
Nearest Stachelhaus code(s):: Cys DLWNLSLIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (5081..5374): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 188.2
Ethylmalonyl-CoA: 175.6
Methoxymalonyl-CoA: 165.0
2-Methylbutyryl-CoA: 125.5
trans-1,2-CPDA: 116.4
Malonyl-CoA: 101.6
Isobutyryl-CoA: 84.2
Propionyl-CoA: 83.2
CHC-CoA: 81.6
Benzoyl-CoA: 60.2
Acetyl-CoA: 53.0
inactive: 50.6
fatty_acid: 31.1
3-Methylbutyryl-CoA: 30.9

Link to PARAS substrate predictor