BGC0001344 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001344 (Cystobacter sp. SBCb004)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	51,513	tubulysin A/tubulysin B/tubulysin C/tubulysin D/tubulysin E/tubulysin F/tubulysin G/tubulysin H/tubulysin I	NRPS:Type I+PKS	100%

BGC0001344 - Region 1 - NRPS,T1PKS

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Location: 1 - 51,513 nt. (total: 51,513 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 51,514
Label		BGC0001344

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001344	2.00	NRP, Polyketide	tubulysin A, tubulysin B, tubulysin C, tubulysin D, tubulysin E, tubulysin F, tubulysin G, tubulysin H, tubulysin I	Cystobacter sp. SBCb004
BGC0000465	0.80	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0001434	0.79	NRP	nematophin	Xenorhabdus budapestensis
BGC0000416	0.72	NRP	rhabdopeptide 1, rhabdopeptide 2, rhabdopeptide 3, rhabdopeptide 4	Xenorhabdus nematophila ATCC 19061
BGC0002071	0.71	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001133	0.70	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001053	0.70	NRP, Polyketide	tubulysin A	Archangium disciforme
BGC0000426	0.67	NRP	sevadicin	Paenibacillus larvae
BGC0000313	0.65	NRP	beauvericin	Beauveria bassiana
BGC0000342	0.64	NRP	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001344	1.00	NRP, Polyketide	tubulysin A, tubulysin B, tubulysin C, tubulysin D, tubulysin E, tubulysin F, tubulysin G, tubulysin H, tubulysin I	Cystobacter sp. SBCb004
BGC0000465	0.72	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0001434	0.72	NRP	nematophin	Xenorhabdus budapestensis
BGC0000416	0.69	NRP	rhabdopeptide 1, rhabdopeptide 2, rhabdopeptide 3, rhabdopeptide 4	Xenorhabdus nematophila ATCC 19061
BGC0002071	0.68	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001133	0.68	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001053	0.67	NRP, Polyketide	tubulysin A	Archangium disciforme
BGC0000426	0.66	NRP	sevadicin	Paenibacillus larvae
BGC0000313	0.66	NRP	beauvericin	Beauveria bassiana
BGC0000342	0.65	NRP	enniatin	Fusarium equiseti

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 51513:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-Hpr) + (Ile - NMe-Val) + (redmal - Cys) + (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

tubB

Search NORINE for peptide: strict or relaxed

AMP-binding (518..921): Hpr

nrpys: Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DIQYIAQVVK
Nearest Stachelhaus code(s):: Hpr DIQYIAQVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

tubC

Search NORINE for peptide: strict or relaxed

AMP-binding (540..936): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGVTFK
Nearest Stachelhaus code(s):: Ile DAFFLGVTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1590..1988): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

tubD

Search NORINE for peptide: strict or relaxed

PKS_AT (536..825): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 150.1
inactive: 80.7
Methoxymalonyl-CoA: 65.4
Methylmalonyl-CoA: 57.9
Isobutyryl-CoA: 51.0
Propionyl-CoA: 37.3
Benzoyl-CoA: 35.4
Ethylmalonyl-CoA: 31.6
CHC-CoA: 29.5
fatty_acid: 28.3
2-Methylbutyryl-CoA: 27.4
Acetyl-CoA: 16.1
trans-1,2-CPDA: 14.3
3-Methylbutyryl-CoA: 6.2

AMP-binding (2653..3044): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLQNMSLIWK
Nearest Stachelhaus code(s):: Cys DLQNMSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

tubE

CAL_domain (532..959): pk

minowa: NH2

Prediction, score:

NH2: 12.8
AHBA: 12.6
fatty_acid: 4.0
shikimic_acid: 0.0
Acetyl-CoA: 0.0

tubF

PKS_AT (532..812): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 138.8
inactive: 79.9
Methylmalonyl-CoA: 63.2
Methoxymalonyl-CoA: 58.4
Isobutyryl-CoA: 40.5
Propionyl-CoA: 33.0
Ethylmalonyl-CoA: 31.1
2-Methylbutyryl-CoA: 21.4
fatty_acid: 19.3
trans-1,2-CPDA: 18.4
Benzoyl-CoA: 13.3
CHC-CoA: 8.2
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0