BGC0001348 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001348 (Streptomyces varsoviensis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	70,801	bafilomycin B1	PKS:Type I	83%

BGC0001348 - Region 1 - T1PKS

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Location: 1 - 70,801 nt. (total: 70,801 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 70,802
Label		BGC0001348

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001348	1.00	Polyketide	JBIR-100	Streptomyces varsoviensis
BGC0000016	0.40	Other	amphotericin B	Streptomyces nodosus
BGC0000018	0.38	Polyketide	angolamycin	Streptomyces eurythermus
BGC0000028	0.37	Polyketide	bafilomycin B1	Streptomyces lohii
BGC0000041	0.33	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001273	0.31	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001163	0.31	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000122	0.31	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001164	0.31	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000996	0.31	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001348	1.00	Polyketide	JBIR-100	Streptomyces varsoviensis
BGC0000016	0.72	Other	amphotericin B	Streptomyces nodosus
BGC0000018	0.71	Polyketide	angolamycin	Streptomyces eurythermus
BGC0000028	0.69	Polyketide	bafilomycin B1	Streptomyces lohii
BGC0000041	0.67	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001273	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001163	0.65	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000122	0.64	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001164	0.64	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000996	0.64	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 70801:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (emal) + (Me-ohmal - ohmal - Me-mal) + (Me-ohmal - ohemal - ccmal) + (pk - pk) + (Me-ohmal - pk) + (Me-ohmal) + (pk)
Non-colinear ordering:: IF55_RS40995 + IF55_RS32365 + IF55_RS32370 + IF55_RS32375 + IF55_RS41000 + IF55_RS36525 + IF55_RS32385

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IF55_RS40995

PKS_AT (0..286): emal

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 75.0%; Methoxymalonyl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 137.8
2-Methylbutyryl-CoA: 135.3
Methylmalonyl-CoA: 133.7
Methoxymalonyl-CoA: 112.5
Propionyl-CoA: 110.1
CHC-CoA: 78.2
trans-1,2-CPDA: 71.7
Malonyl-CoA: 69.4
Isobutyryl-CoA: 64.1
Benzoyl-CoA: 35.7
3-Methylbutyryl-CoA: 26.5
Acetyl-CoA: 25.3
inactive: 16.7
fatty_acid: 11.5

IF55_RS32365

PKS_AT (0..196): mmal

ATSignature: (unknown)

No matches above 50%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 115.0
Ethylmalonyl-CoA: 113.2
Methoxymalonyl-CoA: 84.2
2-Methylbutyryl-CoA: 62.2
trans-1,2-CPDA: 54.6
Malonyl-CoA: 53.3
Propionyl-CoA: 46.2
Isobutyryl-CoA: 41.9
Benzoyl-CoA: 39.8
CHC-CoA: 31.9
3-Methylbutyryl-CoA: 22.6
Acetyl-CoA: 19.7
fatty_acid: 15.5
inactive: 13.2

PKS_AT (1512..1815): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.5
inactive: 114.3
Methoxymalonyl-CoA: 66.9
Methylmalonyl-CoA: 56.5
Propionyl-CoA: 44.2
Isobutyryl-CoA: 32.8
Ethylmalonyl-CoA: 18.4
2-Methylbutyryl-CoA: 16.9
Acetyl-CoA: 16.4
CHC-CoA: 14.4
Benzoyl-CoA: 14.3
fatty_acid: 9.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3230..3526): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 83.3%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.1
Ethylmalonyl-CoA: 196.6
Methoxymalonyl-CoA: 157.6
2-Methylbutyryl-CoA: 139.9
trans-1,2-CPDA: 129.5
CHC-CoA: 111.0
Propionyl-CoA: 103.0
Malonyl-CoA: 77.8
Benzoyl-CoA: 73.0
Isobutyryl-CoA: 67.6
3-Methylbutyryl-CoA: 42.3
Acetyl-CoA: 37.7
fatty_acid: 26.4
inactive: 25.6

IF55_RS32370

PKS_AT (611..858): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 167.4
Methoxymalonyl-CoA: 142.8
Propionyl-CoA: 96.9
CHC-CoA: 82.7
Ethylmalonyl-CoA: 79.5
2-Methylbutyryl-CoA: 78.1
Isobutyryl-CoA: 77.3
trans-1,2-CPDA: 54.4
Benzoyl-CoA: 44.5
Malonyl-CoA: 41.9
Acetyl-CoA: 22.1
3-Methylbutyryl-CoA: 17.0
fatty_acid: 14.1
inactive: 0.0

PKS_AT (2166..2463): emal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 95.8%; Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 95.8%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 221.1
Methylmalonyl-CoA: 207.0
Methoxymalonyl-CoA: 183.2
2-Methylbutyryl-CoA: 136.3
trans-1,2-CPDA: 128.1
CHC-CoA: 111.5
Propionyl-CoA: 99.5
Malonyl-CoA: 92.8
Benzoyl-CoA: 74.6
Isobutyryl-CoA: 70.3
3-Methylbutyryl-CoA: 38.1
Acetyl-CoA: 30.9
fatty_acid: 25.5
inactive: 25.0

PKS_AT (3754..4054): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.8
inactive: 93.4
Methoxymalonyl-CoA: 60.4
Methylmalonyl-CoA: 49.2
Propionyl-CoA: 29.7
Ethylmalonyl-CoA: 25.6
Acetyl-CoA: 20.8
2-Methylbutyryl-CoA: 19.5
CHC-CoA: 17.6
Isobutyryl-CoA: 14.5
Benzoyl-CoA: 13.3
fatty_acid: 8.5
trans-1,2-CPDA: 8.3
3-Methylbutyryl-CoA: 0.0

IF55_RS32375

PKS_AT (566..864): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 230.1
Methylmalonyl-CoA: 229.7
Methoxymalonyl-CoA: 188.4
2-Methylbutyryl-CoA: 172.0
trans-1,2-CPDA: 144.2
CHC-CoA: 132.3
Propionyl-CoA: 126.0
Malonyl-CoA: 115.6
Isobutyryl-CoA: 103.2
Benzoyl-CoA: 101.1
Acetyl-CoA: 56.9
3-Methylbutyryl-CoA: 43.9
inactive: 40.2
fatty_acid: 35.9

PKS_AT (2328..2588): pk

ATSignature: (unknown)

No matches above 50%

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 28.5
fatty_acid: 18.9
Ethylmalonyl-CoA: 13.0
2-Methylbutyryl-CoA: 12.5
Methylmalonyl-CoA: 12.2
Methoxymalonyl-CoA: 9.5
Propionyl-CoA: 8.3
trans-1,2-CPDA: 4.8
CHC-CoA: 3.8
Isobutyryl-CoA: 2.8
Malonyl-CoA: 2.4
Benzoyl-CoA: 2.0
inactive: 0.0
3-Methylbutyryl-CoA: -3.0

IF55_RS41000

PKS_AT (566..869): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 223.2
Ethylmalonyl-CoA: 222.6
Methoxymalonyl-CoA: 182.4
2-Methylbutyryl-CoA: 153.6
Malonyl-CoA: 114.4
trans-1,2-CPDA: 112.5
Propionyl-CoA: 111.7
Isobutyryl-CoA: 107.2
CHC-CoA: 95.9
Benzoyl-CoA: 81.8
Acetyl-CoA: 66.8
inactive: 46.9
3-Methylbutyryl-CoA: 42.8
fatty_acid: 30.9

PKS_AT (2171..2468): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 224.9
Methylmalonyl-CoA: 218.5
Methoxymalonyl-CoA: 177.6
2-Methylbutyryl-CoA: 153.4
trans-1,2-CPDA: 115.3
CHC-CoA: 107.7
Propionyl-CoA: 107.6
Malonyl-CoA: 86.1
Isobutyryl-CoA: 81.8
Benzoyl-CoA: 66.0
Acetyl-CoA: 56.6
3-Methylbutyryl-CoA: 31.0
fatty_acid: 26.2
inactive: 21.2

IF55_RS36525

PKS_AT (561..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 223.2
Ethylmalonyl-CoA: 222.6
Methoxymalonyl-CoA: 182.4
2-Methylbutyryl-CoA: 153.6
Malonyl-CoA: 114.4
trans-1,2-CPDA: 112.5
Propionyl-CoA: 111.7
Isobutyryl-CoA: 107.2
CHC-CoA: 95.9
Benzoyl-CoA: 81.8
Acetyl-CoA: 66.8
inactive: 46.9
3-Methylbutyryl-CoA: 42.8
fatty_acid: 30.9

IF55_RS32385

PKS_AT (566..862): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; Ethylmalonyl-CoA: 54.2%; Methoxymalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 134.8
Methoxymalonyl-CoA: 134.3
Ethylmalonyl-CoA: 117.3
2-Methylbutyryl-CoA: 94.3
Malonyl-CoA: 93.3
Propionyl-CoA: 89.4
trans-1,2-CPDA: 87.5
CHC-CoA: 68.9
Isobutyryl-CoA: 49.7
fatty_acid: 44.8
inactive: 26.4
Acetyl-CoA: 25.3
Benzoyl-CoA: 25.1
3-Methylbutyryl-CoA: 14.3

IF55_RS32430

PKS_AT (15..276): mxmal

ATSignature: (unknown)

No matches above 50%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 13.0
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

IF55_RS32435

AMP-binding (103..316): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IHWNRS-FGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)