BGC0001358 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001358 (Purpureocillium lilacinum)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	99,575	leucinostatin A/leucinostatin B	NRPS:Type I+PKS	100%

BGC0001358 - Region 1 - NRPS,T1PKS

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Location: 1 - 99,575 nt. (total: 99,575 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 99,576
Label		BGC0001358

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001358	1.09	NRP, Polyketide	leucinostatin A, leucinostatin B	Purpureocillium lilacinum
BGC0001137	0.47	Alkaloid	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0001825	0.42	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0001831	0.42	Polyketide	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0001636	0.41	NRP	KK-1	Curvularia clavata
BGC0002186	0.41	NRP, Polyketide	fusaristatin A	Fusarium graminearum PH-1
BGC0002474	0.40	NRP	agrobactin	Agrobacterium tumefaciens
BGC0001399	0.40	NRP	fellutamide B	Aspergillus nidulans FGSC A4
BGC0000397	0.40	NRP	nostocyclopeptide A2	Nostoc sp. ATCC 53789
BGC0001168	0.38	NRP	livipeptin	Streptomyces lividans 1326

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001358	1.00	NRP, Polyketide	leucinostatin A, leucinostatin B	Purpureocillium lilacinum
BGC0002525	0.69	Polyketide	fusarubin, 1233A, 1233B, NG-391, lucilactaene	Fusarium sp.
BGC0001262	0.68	Polyketide	AK-toxin I	Alternaria alternata
BGC0001893	0.67	Other	abscisic acid	Botrytis cinerea B05.10
BGC0001528	0.66	Terpene	betaestacin	Phoma betae
BGC0002194	0.66	Polyketide	epipyrone A	Epicoccum nigrum
BGC0002670	0.66	Other	fosfonochlorin	Fusarium oxysporum f. sp. lycopersici 4287
BGC0002515	0.66	Polyketide	solanapyrone A	Ascochyta rabiei
BGC0002245	0.65	Terpene	terpestacin, preterpestacin 3, preterpestacin 2, preterpestacin 1	Bipolaris maydis C5
BGC0002191	0.65	Polyketide	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 958 - 99575:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Me-pk) + (X - X - X - X - Leu - Leu - X - X - bAla - Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 958 - 68967:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Me-pk) + (X - X - X - X - Leu - Leu - X - X - bAla - Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 43669 - 99575:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (X - X - X - X - Leu - Leu - X - X - bAla - Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

VFPBJ_02527

PKS_AT (581..887): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 54.2%; Propionyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 66.3
Methoxymalonyl-CoA: 45.3
Propionyl-CoA: 45.1
Isobutyryl-CoA: 36.5
Malonyl-CoA: 33.6
2-Methylbutyryl-CoA: 24.4
trans-1,2-CPDA: 22.2
fatty_acid: 20.6
Benzoyl-CoA: 10.4
inactive: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.2

VFPBJ_02532

PKS_AT (446..708): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 62.5%; Methoxymalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 77.9
Methoxymalonyl-CoA: 65.7
Malonyl-CoA: 59.9
Ethylmalonyl-CoA: 50.7
fatty_acid: 32.6
Isobutyryl-CoA: 28.0
Propionyl-CoA: 26.4
2-Methylbutyryl-CoA: 25.9
CHC-CoA: 18.8
trans-1,2-CPDA: 18.7
Benzoyl-CoA: 18.3
inactive: 16.2
3-Methylbutyryl-CoA: 10.9
Acetyl-CoA: 9.1

VFPBJ_02533

AMP-binding (0..243): X

nrpys: meHex

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SLVWAGGGVK
Nearest Stachelhaus code(s):: meHex SLVWAGGGVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

VFPBJ_02539

Search NORINE for peptide: strict or relaxed

AMP-binding (708..1115): X

nrpys: 4Me-Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVFYVMAAAK
Nearest Stachelhaus code(s):: 4Me-Pro DVFYVMAAAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1809..2212): X

nrpys: nohme-oxoDec

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAIGIGGVIK
Nearest Stachelhaus code(s):: nohme-oxoDec DAIGIGGVIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2913..3316): X

nrpys: 3OH-Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAILVGAIVK
Nearest Stachelhaus code(s):: 3OH-Leu DAILVGAIVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4043..4447): X

nrpys: Aib

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: iva (Ival)

Stachelhaus prediction details:

Stachelhaus sequence:: DLGFLAGLFK
Nearest Stachelhaus code(s):: Aib DLGFLAGLFK (100% 8Å match); Abu or Ival DLGFLAGLFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5157..5571): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASLVGAVLK
Nearest Stachelhaus code(s):: Leu DASLVGAVLK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6253..6657): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALLVGAVAK
Nearest Stachelhaus code(s):: Leu DALLVGAVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7417..7828): X

nrpys: Aib

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: iva (Ival)

Stachelhaus prediction details:

Stachelhaus sequence:: DLGFLAGVFK
Nearest Stachelhaus code(s):: Aib DLGFLAGVFK (100% 8Å match); Abu or Ival DLGFLAGVFK (97% 8Å match); Leu DLGFLAGVFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (8558..8938): X

nrpys: Aib

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EISILIAFAK
Nearest Stachelhaus code(s):: Aib EISILIAFAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (9662..10068): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVAMVVAMFK
Nearest Stachelhaus code(s):: bAla DVAMVVAMFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (10799..11198): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVGMVGGIYK
Nearest Stachelhaus code(s):: Ala DVGMVGGIYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)